Experiment: MoYLS4 with DETA/NO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0123 and DVU0124 are separated by 46 nucleotides DVU0124 and DVU0125 are separated by 202 nucleotides DVU0125 and b3486 overlap by 4 nucleotides
DVU0123: DVU0123 - membrane protein, putative (TIGR), at 156,693 to 157,775
DVU0123
DVU0124: DVU0124 - hypothetical protein (TIGR), at 157,822 to 158,010
DVU0124
DVU0125: DVU0125 - hypothetical protein (TIGR), at 158,213 to 159,346
DVU0125
DVU0126: b3486 - ABC transporter, ATP-binding protein (TIGR), at 159,343 to 162,858
b3486
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 157.254 kb on + strand, within DVU0123 at 157.357 kb on + strand, within DVU0123 at 157.365 kb on - strand, within DVU0123 at 157.405 kb on + strand, within DVU0123 at 157.423 kb on + strand, within DVU0123 at 157.433 kb on - strand, within DVU0123 at 157.455 kb on - strand, within DVU0123 at 157.501 kb on + strand, within DVU0123 at 157.529 kb on + strand, within DVU0123 at 157.537 kb on - strand, within DVU0123 at 157.569 kb on - strand, within DVU0123 at 157.621 kb on + strand, within DVU0123 at 157.629 kb on - strand, within DVU0123 at 157.629 kb on - strand, within DVU0123 at 157.640 kb on + strand, within DVU0123 at 157.653 kb on - strand, within DVU0123 at 157.694 kb on - strand at 157.731 kb on + strand at 157.782 kb on + strand at 158.000 kb on - strand at 158.007 kb on - strand at 158.022 kb on + strand at 158.022 kb on + strand at 158.023 kb on - strand at 158.156 kb on + strand at 158.159 kb on - strand at 158.164 kb on - strand at 158.169 kb on + strand at 158.169 kb on + strand at 158.177 kb on - strand at 158.184 kb on + strand at 158.254 kb on + strand at 158.262 kb on - strand at 158.357 kb on - strand, within DVU0125 at 158.386 kb on - strand, within DVU0125 at 158.428 kb on + strand, within DVU0125 at 158.443 kb on + strand, within DVU0125 at 158.486 kb on + strand, within DVU0125 at 158.503 kb on - strand, within DVU0125 at 158.525 kb on - strand, within DVU0125 at 158.579 kb on + strand, within DVU0125 at 158.698 kb on + strand, within DVU0125 at 158.706 kb on - strand, within DVU0125 at 158.706 kb on - strand, within DVU0125 at 158.706 kb on - strand, within DVU0125 at 158.816 kb on - strand, within DVU0125 at 158.908 kb on - strand, within DVU0125 at 158.972 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.084 kb on + strand, within DVU0125 at 159.092 kb on - strand, within DVU0125 at 159.092 kb on - strand, within DVU0125 at 159.092 kb on - strand, within DVU0125 at 159.099 kb on + strand, within DVU0125 at 159.101 kb on + strand, within DVU0125 at 159.135 kb on + strand, within DVU0125 at 159.136 kb on + strand, within DVU0125 at 159.171 kb on + strand, within DVU0125 at 159.194 kb on + strand, within DVU0125 at 159.220 kb on + strand, within DVU0125 at 159.228 kb on - strand, within DVU0125 at 159.279 kb on + strand at 159.290 kb on + strand at 159.290 kb on + strand at 159.408 kb on - strand at 159.431 kb on - strand at 159.469 kb on - strand at 159.549 kb on + strand at 159.572 kb on - strand at 159.584 kb on + strand at 159.649 kb on - strand at 159.772 kb on + strand, within b3486 at 159.780 kb on - strand, within b3486 at 159.810 kb on - strand, within b3486 at 159.810 kb on - strand, within b3486 at 159.817 kb on + strand, within b3486 at 159.833 kb on - strand, within b3486 at 159.836 kb on + strand, within b3486 at 159.836 kb on + strand, within b3486 at 159.866 kb on - strand, within b3486 at 159.940 kb on + strand, within b3486 at 159.985 kb on - strand, within b3486 at 159.990 kb on + strand, within b3486 at 159.990 kb on + strand, within b3486 at 159.998 kb on - strand, within b3486 at 160.008 kb on - strand, within b3486 at 160.021 kb on - strand, within b3486 at 160.057 kb on - strand, within b3486 at 160.057 kb on - strand, within b3486 at 160.057 kb on - strand, within b3486 at 160.062 kb on + strand, within b3486 at 160.129 kb on - strand, within b3486 at 160.142 kb on - strand, within b3486 at 160.155 kb on - strand, within b3486 at 160.155 kb on - strand, within b3486 at 160.156 kb on + strand, within b3486 at 160.158 kb on + strand, within b3486 at 160.159 kb on + strand, within b3486 at 160.192 kb on + strand, within b3486 at 160.227 kb on + strand, within b3486 at 160.227 kb on + strand, within b3486 at 160.235 kb on - strand, within b3486 at 160.235 kb on - strand, within b3486 at 160.235 kb on - strand, within b3486 at 160.237 kb on + strand, within b3486 at 160.245 kb on - strand, within b3486 at 160.245 kb on - strand, within b3486
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DETA/NO 100uM remove 157,254 + DVU0123 0.52 -0.1 157,357 + DVU0123 0.61 -0.4 157,365 - DVU0123 0.62 +0.1 157,405 + DVU0123 0.66 +0.2 157,423 + DVU0123 0.67 -0.3 157,433 - DVU0123 0.68 +0.0 157,455 - DVU0123 0.70 -0.4 157,501 + DVU0123 0.75 -0.0 157,529 + DVU0123 0.77 +0.0 157,537 - DVU0123 0.78 +1.2 157,569 - DVU0123 0.81 +0.1 157,621 + DVU0123 0.86 -2.0 157,629 - DVU0123 0.86 +0.3 157,629 - DVU0123 0.86 -0.2 157,640 + DVU0123 0.87 -0.3 157,653 - DVU0123 0.89 +0.6 157,694 - -0.0 157,731 + +0.8 157,782 + -0.4 158,000 - -0.1 158,007 - +0.2 158,022 + +0.2 158,022 + +0.9 158,023 - -0.1 158,156 + -1.1 158,159 - -0.2 158,164 - +0.0 158,169 + +1.4 158,169 + +0.1 158,177 - +0.3 158,184 + +1.7 158,254 + -0.4 158,262 - +1.6 158,357 - DVU0125 0.13 -0.3 158,386 - DVU0125 0.15 +0.4 158,428 + DVU0125 0.19 -0.9 158,443 + DVU0125 0.20 +0.1 158,486 + DVU0125 0.24 +1.2 158,503 - DVU0125 0.26 -0.2 158,525 - DVU0125 0.28 -0.6 158,579 + DVU0125 0.32 +0.2 158,698 + DVU0125 0.43 -0.1 158,706 - DVU0125 0.43 -1.9 158,706 - DVU0125 0.43 +0.9 158,706 - DVU0125 0.43 -0.2 158,816 - DVU0125 0.53 -0.1 158,908 - DVU0125 0.61 -0.1 158,972 + DVU0125 0.67 -0.2 159,084 + DVU0125 0.77 -0.9 159,084 + DVU0125 0.77 -1.3 159,084 + DVU0125 0.77 -0.1 159,084 + DVU0125 0.77 -0.6 159,092 - DVU0125 0.78 +0.2 159,092 - DVU0125 0.78 -0.4 159,092 - DVU0125 0.78 -0.6 159,099 + DVU0125 0.78 -0.6 159,101 + DVU0125 0.78 -0.2 159,135 + DVU0125 0.81 -0.1 159,136 + DVU0125 0.81 -0.6 159,171 + DVU0125 0.84 +0.0 159,194 + DVU0125 0.87 -0.0 159,220 + DVU0125 0.89 -0.1 159,228 - DVU0125 0.90 +0.0 159,279 + +1.1 159,290 + +0.0 159,290 + -1.3 159,408 - -0.5 159,431 - -0.1 159,469 - +0.3 159,549 + -0.3 159,572 - -0.1 159,584 + -0.8 159,649 - -0.9 159,772 + b3486 DVU0126 0.12 +0.0 159,780 - b3486 DVU0126 0.12 -1.2 159,810 - b3486 DVU0126 0.13 -0.5 159,810 - b3486 DVU0126 0.13 -0.9 159,817 + b3486 DVU0126 0.13 +2.8 159,833 - b3486 DVU0126 0.14 +0.4 159,836 + b3486 DVU0126 0.14 +0.0 159,836 + b3486 DVU0126 0.14 -0.6 159,866 - b3486 DVU0126 0.15 -0.6 159,940 + b3486 DVU0126 0.17 +0.1 159,985 - b3486 DVU0126 0.18 -0.2 159,990 + b3486 DVU0126 0.18 -0.2 159,990 + b3486 DVU0126 0.18 -0.6 159,998 - b3486 DVU0126 0.19 -0.2 160,008 - b3486 DVU0126 0.19 -0.1 160,021 - b3486 DVU0126 0.19 +1.4 160,057 - b3486 DVU0126 0.20 -0.4 160,057 - b3486 DVU0126 0.20 +0.6 160,057 - b3486 DVU0126 0.20 -0.1 160,062 + b3486 DVU0126 0.20 -0.0 160,129 - b3486 DVU0126 0.22 -0.5 160,142 - b3486 DVU0126 0.23 -0.7 160,155 - b3486 DVU0126 0.23 -0.5 160,155 - b3486 DVU0126 0.23 -0.4 160,156 + b3486 DVU0126 0.23 -0.6 160,158 + b3486 DVU0126 0.23 -1.1 160,159 + b3486 DVU0126 0.23 -0.9 160,192 + b3486 DVU0126 0.24 -2.4 160,227 + b3486 DVU0126 0.25 +0.4 160,227 + b3486 DVU0126 0.25 -0.5 160,235 - b3486 DVU0126 0.25 -0.5 160,235 - b3486 DVU0126 0.25 +0.9 160,235 - b3486 DVU0126 0.25 +1.6 160,237 + b3486 DVU0126 0.25 -0.0 160,245 - b3486 DVU0126 0.26 -1.0 160,245 - b3486 DVU0126 0.26 -0.1
Or see this region's nucleotide sequence