Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3634 and Rru_A3635 overlap by 4 nucleotides Rru_A3635 and Rru_A3636 are separated by 25 nucleotides Rru_A3636 and Rru_A3637 are separated by 264 nucleotides
Rru_A3634: Rru_A3634 - Protein of unknown function UPF0001 (NCBI), at 4,175,262 to 4,175,990
_A3634
Rru_A3635: Rru_A3635 - hypothetical protein (NCBI), at 4,175,987 to 4,176,490
_A3635
Rru_A3636: Rru_A3636 - hypothetical protein (NCBI), at 4,176,516 to 4,177,910
_A3636
Rru_A3637: Rru_A3637 - GTP cyclohydrolase II (NCBI), at 4,178,175 to 4,179,329
_A3637
Position (kb) 4176
4177
4178 Strain fitness (log2 ratio) -1
0
1
2 at 4175.684 kb on + strand, within Rru_A3634 at 4176.149 kb on - strand, within Rru_A3635 at 4176.239 kb on - strand, within Rru_A3635 at 4176.239 kb on - strand, within Rru_A3635 at 4176.240 kb on + strand, within Rru_A3635 at 4176.241 kb on - strand, within Rru_A3635 at 4176.514 kb on + strand at 4176.515 kb on - strand at 4176.515 kb on - strand at 4176.568 kb on + strand at 4176.828 kb on - strand, within Rru_A3636 at 4176.828 kb on - strand, within Rru_A3636 at 4176.828 kb on - strand, within Rru_A3636 at 4176.967 kb on + strand, within Rru_A3636 at 4176.967 kb on + strand, within Rru_A3636 at 4177.231 kb on + strand, within Rru_A3636 at 4177.232 kb on - strand, within Rru_A3636 at 4177.379 kb on - strand, within Rru_A3636 at 4177.379 kb on - strand, within Rru_A3636 at 4177.561 kb on + strand, within Rru_A3636 at 4178.100 kb on + strand at 4178.100 kb on + strand at 4178.101 kb on - strand at 4178.163 kb on + strand at 4178.164 kb on - strand at 4178.164 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 4,175,684 + Rru_A3634 0.58 -0.3 4,176,149 - Rru_A3635 0.32 +0.2 4,176,239 - Rru_A3635 0.50 +0.9 4,176,239 - Rru_A3635 0.50 -0.3 4,176,240 + Rru_A3635 0.50 -0.0 4,176,241 - Rru_A3635 0.50 -0.3 4,176,514 + +0.3 4,176,515 - +2.3 4,176,515 - +0.4 4,176,568 + -0.1 4,176,828 - Rru_A3636 0.22 +0.1 4,176,828 - Rru_A3636 0.22 -0.8 4,176,828 - Rru_A3636 0.22 -0.8 4,176,967 + Rru_A3636 0.32 +0.9 4,176,967 + Rru_A3636 0.32 -0.4 4,177,231 + Rru_A3636 0.51 -1.0 4,177,232 - Rru_A3636 0.51 +0.2 4,177,379 - Rru_A3636 0.62 -1.1 4,177,379 - Rru_A3636 0.62 -0.1 4,177,561 + Rru_A3636 0.75 -0.4 4,178,100 + +0.2 4,178,100 + +1.0 4,178,101 - -0.1 4,178,163 + -0.0 4,178,164 - -0.1 4,178,164 - -0.9
Or see this region's nucleotide sequence