Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A3065 and Rru_A3066 are separated by 163 nucleotides Rru_A3066 and Rru_A3067 are separated by 11 nucleotides Rru_A3067 and Rru_A3068 are separated by 75 nucleotides Rru_A3068 and Rru_A3069 are separated by 4 nucleotides Rru_A3069 and Rru_A3070 are separated by 309 nucleotides
Rru_A3065: Rru_A3065 - Transcriptional Regulator, XRE family (NCBI), at 3,531,969 to 3,532,265
_A3065
Rru_A3066: Rru_A3066 - NAD(P)H dehydrogenase (quinone) (NCBI), at 3,532,429 to 3,533,025
_A3066
Rru_A3067: Rru_A3067 - Transcriptional Regulator, TetR family (NCBI), at 3,533,037 to 3,533,636
_A3067
Rru_A3068: Rru_A3068 - Plasmid maintenance system antidote protein (NCBI), at 3,533,712 to 3,533,999
_A3068
Rru_A3069: Rru_A3069 - Plasmid maintenance system killer (NCBI), at 3,534,004 to 3,534,282
_A3069
Rru_A3070: Rru_A3070 - Transcriptional Regulator, AsnC family (NCBI), at 3,534,592 to 3,535,068
_A3070
Position (kb)
3533
3534 Strain fitness (log2 ratio)
-2
-1
0
1 at 3532.206 kb on + strand, within Rru_A3065 at 3532.206 kb on + strand, within Rru_A3065 at 3532.207 kb on - strand, within Rru_A3065 at 3532.269 kb on + strand at 3532.271 kb on + strand at 3532.271 kb on + strand at 3532.283 kb on + strand at 3532.284 kb on - strand at 3532.284 kb on - strand at 3532.681 kb on + strand, within Rru_A3066 at 3532.681 kb on + strand, within Rru_A3066 at 3532.682 kb on - strand, within Rru_A3066 at 3532.690 kb on + strand, within Rru_A3066 at 3532.691 kb on - strand, within Rru_A3066 at 3532.691 kb on - strand, within Rru_A3066 at 3532.756 kb on + strand, within Rru_A3066 at 3532.861 kb on + strand, within Rru_A3066 at 3532.861 kb on + strand, within Rru_A3066 at 3532.862 kb on - strand, within Rru_A3066 at 3532.896 kb on + strand, within Rru_A3066 at 3532.896 kb on + strand, within Rru_A3066 at 3532.897 kb on - strand, within Rru_A3066 at 3532.897 kb on - strand, within Rru_A3066 at 3532.897 kb on - strand, within Rru_A3066 at 3532.898 kb on + strand, within Rru_A3066 at 3532.899 kb on - strand, within Rru_A3066 at 3532.925 kb on + strand, within Rru_A3066 at 3533.199 kb on + strand, within Rru_A3067 at 3533.199 kb on + strand, within Rru_A3067 at 3533.199 kb on + strand, within Rru_A3067 at 3533.199 kb on + strand, within Rru_A3067 at 3533.199 kb on + strand, within Rru_A3067 at 3533.199 kb on + strand, within Rru_A3067 at 3533.200 kb on - strand, within Rru_A3067 at 3533.200 kb on - strand, within Rru_A3067 at 3533.200 kb on - strand, within Rru_A3067 at 3533.200 kb on - strand, within Rru_A3067 at 3533.200 kb on - strand, within Rru_A3067 at 3533.200 kb on - strand, within Rru_A3067 at 3533.241 kb on + strand, within Rru_A3067 at 3533.242 kb on - strand, within Rru_A3067 at 3533.250 kb on + strand, within Rru_A3067 at 3533.251 kb on - strand, within Rru_A3067 at 3533.251 kb on - strand, within Rru_A3067 at 3533.251 kb on - strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.340 kb on + strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.341 kb on - strand, within Rru_A3067 at 3533.423 kb on - strand, within Rru_A3067 at 3533.434 kb on - strand, within Rru_A3067 at 3533.606 kb on + strand at 3533.607 kb on - strand at 3533.607 kb on - strand at 3533.634 kb on + strand at 3533.634 kb on + strand at 3533.635 kb on - strand at 3533.688 kb on - strand at 3534.011 kb on + strand at 3534.032 kb on + strand, within Rru_A3069 at 3534.032 kb on + strand, within Rru_A3069 at 3534.033 kb on - strand, within Rru_A3069 at 3534.033 kb on - strand, within Rru_A3069 at 3534.033 kb on - strand, within Rru_A3069 at 3534.033 kb on - strand, within Rru_A3069 at 3534.033 kb on - strand, within Rru_A3069 at 3534.346 kb on - strand at 3534.346 kb on - strand at 3534.395 kb on + strand at 3534.395 kb on + strand at 3534.454 kb on + strand at 3534.454 kb on + strand at 3534.454 kb on + strand at 3534.454 kb on + strand at 3534.492 kb on + strand at 3534.493 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 3,532,206 + Rru_A3065 0.80 +0.9 3,532,206 + Rru_A3065 0.80 -0.4 3,532,207 - Rru_A3065 0.80 -0.2 3,532,269 + +0.4 3,532,271 + -0.5 3,532,271 + -1.3 3,532,283 + +0.3 3,532,284 - +0.9 3,532,284 - -0.5 3,532,681 + Rru_A3066 0.42 -0.0 3,532,681 + Rru_A3066 0.42 -0.2 3,532,682 - Rru_A3066 0.42 +0.5 3,532,690 + Rru_A3066 0.44 -0.9 3,532,691 - Rru_A3066 0.44 -0.1 3,532,691 - Rru_A3066 0.44 -0.2 3,532,756 + Rru_A3066 0.55 +0.0 3,532,861 + Rru_A3066 0.72 -0.3 3,532,861 + Rru_A3066 0.72 -0.2 3,532,862 - Rru_A3066 0.73 -1.4 3,532,896 + Rru_A3066 0.78 -0.5 3,532,896 + Rru_A3066 0.78 -1.2 3,532,897 - Rru_A3066 0.78 +0.2 3,532,897 - Rru_A3066 0.78 -0.3 3,532,897 - Rru_A3066 0.78 -0.4 3,532,898 + Rru_A3066 0.79 -0.2 3,532,899 - Rru_A3066 0.79 -0.0 3,532,925 + Rru_A3066 0.83 -1.4 3,533,199 + Rru_A3067 0.27 +0.7 3,533,199 + Rru_A3067 0.27 -0.8 3,533,199 + Rru_A3067 0.27 -0.1 3,533,199 + Rru_A3067 0.27 -0.7 3,533,199 + Rru_A3067 0.27 -1.2 3,533,199 + Rru_A3067 0.27 -1.0 3,533,200 - Rru_A3067 0.27 -1.5 3,533,200 - Rru_A3067 0.27 +0.4 3,533,200 - Rru_A3067 0.27 -1.4 3,533,200 - Rru_A3067 0.27 -0.6 3,533,200 - Rru_A3067 0.27 -0.3 3,533,200 - Rru_A3067 0.27 -1.3 3,533,241 + Rru_A3067 0.34 -0.8 3,533,242 - Rru_A3067 0.34 +0.9 3,533,250 + Rru_A3067 0.35 -0.6 3,533,251 - Rru_A3067 0.36 -0.2 3,533,251 - Rru_A3067 0.36 -0.8 3,533,251 - Rru_A3067 0.36 -0.4 3,533,340 + Rru_A3067 0.51 -1.6 3,533,340 + Rru_A3067 0.51 -0.9 3,533,340 + Rru_A3067 0.51 +0.9 3,533,340 + Rru_A3067 0.51 -1.9 3,533,340 + Rru_A3067 0.51 -0.1 3,533,340 + Rru_A3067 0.51 -0.8 3,533,340 + Rru_A3067 0.51 -0.5 3,533,340 + Rru_A3067 0.51 -0.6 3,533,341 - Rru_A3067 0.51 -0.6 3,533,341 - Rru_A3067 0.51 -1.1 3,533,341 - Rru_A3067 0.51 -0.3 3,533,341 - Rru_A3067 0.51 +0.9 3,533,341 - Rru_A3067 0.51 -0.6 3,533,341 - Rru_A3067 0.51 -0.4 3,533,341 - Rru_A3067 0.51 -0.4 3,533,341 - Rru_A3067 0.51 -0.9 3,533,423 - Rru_A3067 0.64 -0.3 3,533,434 - Rru_A3067 0.66 -0.9 3,533,606 + -0.9 3,533,607 - -0.8 3,533,607 - -1.2 3,533,634 + -1.1 3,533,634 + -0.4 3,533,635 - +0.0 3,533,688 - -0.7 3,534,011 + +0.9 3,534,032 + Rru_A3069 0.10 +1.0 3,534,032 + Rru_A3069 0.10 -0.1 3,534,033 - Rru_A3069 0.10 -0.3 3,534,033 - Rru_A3069 0.10 +0.0 3,534,033 - Rru_A3069 0.10 -0.6 3,534,033 - Rru_A3069 0.10 -0.3 3,534,033 - Rru_A3069 0.10 -0.3 3,534,346 - -0.2 3,534,346 - -0.2 3,534,395 + -0.3 3,534,395 + +0.2 3,534,454 + -0.4 3,534,454 + -0.1 3,534,454 + -0.6 3,534,454 + +0.9 3,534,492 + +0.1 3,534,493 - -0.9
Or see this region's nucleotide sequence