Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2860 and Rru_A2861 overlap by 4 nucleotides Rru_A2861 and Rru_A2862 overlap by 4 nucleotides Rru_A2862 and Rru_A2863 are separated by 102 nucleotides Rru_A2863 and Rru_A2864 are separated by 166 nucleotides
Rru_A2860: Rru_A2860 - hypothetical protein (NCBI), at 3,294,481 to 3,295,125
_A2860
Rru_A2861: Rru_A2861 - hypothetical protein (NCBI), at 3,295,122 to 3,295,700
_A2861
Rru_A2862: Rru_A2862 - Alpha/beta hydrolase fold (NCBI), at 3,295,697 to 3,296,512
_A2862
Rru_A2863: Rru_A2863 - Transcriptional Regulator, TetR family (NCBI), at 3,296,615 to 3,297,265
_A2863
Rru_A2864: Rru_A2864 - Predicted signal transduction protein containing EFhand domain (NCBI), at 3,297,432 to 3,298,244
_A2864
Position (kb)
3295
3296
3297 Strain fitness (log2 ratio)
-1
0
1 at 3295.039 kb on + strand, within Rru_A2860 at 3295.039 kb on + strand, within Rru_A2860 at 3295.040 kb on - strand, within Rru_A2860 at 3295.631 kb on + strand, within Rru_A2861 at 3295.631 kb on + strand, within Rru_A2861 at 3295.632 kb on - strand, within Rru_A2861 at 3295.729 kb on + strand at 3295.730 kb on - strand at 3295.816 kb on - strand, within Rru_A2862 at 3295.886 kb on + strand, within Rru_A2862 at 3295.887 kb on - strand, within Rru_A2862 at 3295.946 kb on + strand, within Rru_A2862 at 3295.946 kb on + strand, within Rru_A2862 at 3295.946 kb on + strand, within Rru_A2862 at 3295.947 kb on - strand, within Rru_A2862 at 3295.947 kb on - strand, within Rru_A2862 at 3296.026 kb on + strand, within Rru_A2862 at 3296.027 kb on - strand, within Rru_A2862 at 3296.336 kb on + strand, within Rru_A2862 at 3296.336 kb on + strand, within Rru_A2862 at 3296.336 kb on + strand, within Rru_A2862 at 3296.336 kb on + strand, within Rru_A2862 at 3296.337 kb on - strand, within Rru_A2862 at 3296.337 kb on - strand, within Rru_A2862 at 3296.337 kb on - strand, within Rru_A2862 at 3296.337 kb on - strand, within Rru_A2862 at 3296.419 kb on + strand, within Rru_A2862 at 3296.419 kb on + strand, within Rru_A2862 at 3296.419 kb on + strand, within Rru_A2862 at 3296.419 kb on + strand, within Rru_A2862 at 3296.420 kb on - strand, within Rru_A2862 at 3296.420 kb on - strand, within Rru_A2862 at 3296.420 kb on - strand, within Rru_A2862 at 3296.420 kb on - strand, within Rru_A2862 at 3296.510 kb on + strand at 3296.511 kb on - strand at 3296.511 kb on - strand at 3296.617 kb on - strand at 3296.721 kb on + strand, within Rru_A2863 at 3296.721 kb on + strand, within Rru_A2863 at 3296.721 kb on + strand, within Rru_A2863 at 3296.722 kb on - strand, within Rru_A2863 at 3297.011 kb on - strand, within Rru_A2863 at 3297.011 kb on - strand, within Rru_A2863 at 3297.117 kb on + strand, within Rru_A2863 at 3297.117 kb on + strand, within Rru_A2863 at 3297.117 kb on + strand, within Rru_A2863 at 3297.118 kb on - strand, within Rru_A2863 at 3297.144 kb on + strand, within Rru_A2863 at 3297.144 kb on + strand, within Rru_A2863 at 3297.144 kb on + strand, within Rru_A2863 at 3297.144 kb on + strand, within Rru_A2863 at 3297.145 kb on - strand, within Rru_A2863 at 3297.279 kb on + strand at 3297.279 kb on + strand at 3297.279 kb on + strand at 3297.280 kb on - strand at 3297.450 kb on + strand at 3297.450 kb on + strand at 3297.450 kb on + strand at 3297.451 kb on - strand at 3297.451 kb on - strand at 3297.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 3,295,039 + Rru_A2860 0.87 +0.1 3,295,039 + Rru_A2860 0.87 +0.9 3,295,040 - Rru_A2860 0.87 +0.5 3,295,631 + Rru_A2861 0.88 -0.2 3,295,631 + Rru_A2861 0.88 +0.1 3,295,632 - Rru_A2861 0.88 -0.8 3,295,729 + +0.1 3,295,730 - -0.2 3,295,816 - Rru_A2862 0.15 -0.5 3,295,886 + Rru_A2862 0.23 -0.4 3,295,887 - Rru_A2862 0.23 -0.4 3,295,946 + Rru_A2862 0.31 +0.1 3,295,946 + Rru_A2862 0.31 -0.3 3,295,946 + Rru_A2862 0.31 -0.5 3,295,947 - Rru_A2862 0.31 -0.5 3,295,947 - Rru_A2862 0.31 -1.1 3,296,026 + Rru_A2862 0.40 -0.3 3,296,027 - Rru_A2862 0.40 -1.2 3,296,336 + Rru_A2862 0.78 -0.1 3,296,336 + Rru_A2862 0.78 +0.0 3,296,336 + Rru_A2862 0.78 -0.9 3,296,336 + Rru_A2862 0.78 -0.6 3,296,337 - Rru_A2862 0.78 +0.1 3,296,337 - Rru_A2862 0.78 -0.8 3,296,337 - Rru_A2862 0.78 -0.3 3,296,337 - Rru_A2862 0.78 +0.7 3,296,419 + Rru_A2862 0.88 -0.8 3,296,419 + Rru_A2862 0.88 -0.6 3,296,419 + Rru_A2862 0.88 -0.8 3,296,419 + Rru_A2862 0.88 -0.7 3,296,420 - Rru_A2862 0.89 -0.5 3,296,420 - Rru_A2862 0.89 -1.3 3,296,420 - Rru_A2862 0.89 +0.9 3,296,420 - Rru_A2862 0.89 -0.5 3,296,510 + +0.2 3,296,511 - -0.3 3,296,511 - +0.9 3,296,617 - -0.6 3,296,721 + Rru_A2863 0.16 +0.1 3,296,721 + Rru_A2863 0.16 +0.0 3,296,721 + Rru_A2863 0.16 -0.2 3,296,722 - Rru_A2863 0.16 -0.7 3,297,011 - Rru_A2863 0.61 -0.4 3,297,011 - Rru_A2863 0.61 +0.5 3,297,117 + Rru_A2863 0.77 -0.5 3,297,117 + Rru_A2863 0.77 +0.1 3,297,117 + Rru_A2863 0.77 +0.0 3,297,118 - Rru_A2863 0.77 -0.5 3,297,144 + Rru_A2863 0.81 -0.2 3,297,144 + Rru_A2863 0.81 -0.4 3,297,144 + Rru_A2863 0.81 -0.3 3,297,144 + Rru_A2863 0.81 -0.2 3,297,145 - Rru_A2863 0.81 +0.3 3,297,279 + -0.0 3,297,279 + -0.4 3,297,279 + +1.0 3,297,280 - -0.1 3,297,450 + -0.4 3,297,450 + -0.7 3,297,450 + -1.3 3,297,451 - +0.9 3,297,451 - +0.1 3,297,475 - -1.2
Or see this region's nucleotide sequence