Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2799 and Rru_A2800 are separated by 249 nucleotides Rru_A2800 and Rru_A2801 are separated by 74 nucleotides Rru_A2801 and Rru_A2802 are separated by 196 nucleotides Rru_A2802 and Rru_A2803 overlap by 4 nucleotides Rru_A2803 and Rru_A2804 overlap by 20 nucleotides
Rru_A2799: Rru_A2799 - hypothetical protein (NCBI), at 3,232,972 to 3,233,301
_A2799
Rru_A2800: Rru_A2800 - hypothetical protein (NCBI), at 3,233,551 to 3,234,003
_A2800
Rru_A2801: Rru_A2801 - thiol-disulfide interchange protein (NCBI), at 3,234,078 to 3,234,539
_A2801
Rru_A2802: Rru_A2802 - Cytochrome c, class I (NCBI), at 3,234,736 to 3,234,984
_A2802
Rru_A2803: Rru_A2803 - putative thioredoxin (NCBI), at 3,234,981 to 3,235,499
_A2803
Rru_A2804: Rru_A2804 - ABC transporter component (NCBI), at 3,235,480 to 3,236,211
_A2804
Position (kb)
3234
3235 Strain fitness (log2 ratio)
-1
0
1 at 3233.296 kb on - strand at 3233.296 kb on - strand at 3233.316 kb on + strand at 3233.316 kb on + strand at 3233.317 kb on - strand at 3233.317 kb on - strand at 3233.363 kb on + strand at 3233.364 kb on - strand at 3233.534 kb on - strand at 3233.716 kb on + strand, within Rru_A2800 at 3233.774 kb on - strand, within Rru_A2800 at 3233.774 kb on - strand, within Rru_A2800 at 3233.904 kb on + strand, within Rru_A2800 at 3233.908 kb on + strand, within Rru_A2800 at 3233.909 kb on - strand, within Rru_A2800 at 3233.909 kb on - strand, within Rru_A2800 at 3234.079 kb on + strand at 3234.079 kb on + strand at 3234.080 kb on - strand at 3234.080 kb on - strand at 3234.080 kb on - strand at 3234.080 kb on - strand at 3234.587 kb on + strand at 3234.587 kb on + strand at 3234.587 kb on + strand at 3234.587 kb on + strand at 3234.588 kb on - strand at 3235.099 kb on + strand, within Rru_A2803 at 3235.100 kb on - strand, within Rru_A2803 at 3235.100 kb on - strand, within Rru_A2803 at 3235.100 kb on - strand, within Rru_A2803 at 3235.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 3,233,296 - -0.6 3,233,296 - +0.3 3,233,316 + +0.9 3,233,316 + -0.3 3,233,317 - +0.1 3,233,317 - -0.2 3,233,363 + +0.3 3,233,364 - -0.3 3,233,534 - -0.5 3,233,716 + Rru_A2800 0.36 -0.6 3,233,774 - Rru_A2800 0.49 -1.0 3,233,774 - Rru_A2800 0.49 -0.2 3,233,904 + Rru_A2800 0.78 -0.9 3,233,908 + Rru_A2800 0.79 -1.1 3,233,909 - Rru_A2800 0.79 -0.1 3,233,909 - Rru_A2800 0.79 -0.8 3,234,079 + -1.0 3,234,079 + -0.1 3,234,080 - +0.1 3,234,080 - +0.2 3,234,080 - +0.1 3,234,080 - -1.0 3,234,587 + -0.0 3,234,587 + +0.9 3,234,587 + -0.2 3,234,587 + -0.3 3,234,588 - -0.7 3,235,099 + Rru_A2803 0.23 -0.9 3,235,100 - Rru_A2803 0.23 -0.3 3,235,100 - Rru_A2803 0.23 -0.4 3,235,100 - Rru_A2803 0.23 -0.5 3,235,482 - +0.3
Or see this region's nucleotide sequence