Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1809 and Rru_A1810 are separated by 4 nucleotides Rru_A1810 and Rru_A1811 are separated by 195 nucleotides Rru_A1811 and Rru_A1812 overlap by 4 nucleotides Rru_A1812 and Rru_A1813 are separated by 167 nucleotides
Rru_A1809: Rru_A1809 - Sigma-24 (FecI) (NCBI), at 2,105,982 to 2,106,527
_A1809
Rru_A1810: Rru_A1810 - hypothetical protein (NCBI), at 2,106,532 to 2,106,966
_A1810
Rru_A1811: Rru_A1811 - Beta-lactamase-like (NCBI), at 2,107,162 to 2,108,010
_A1811
Rru_A1812: Rru_A1812 - Protein of unknown function DUF62 (NCBI), at 2,108,007 to 2,108,765
_A1812
Rru_A1813: Rru_A1813 - Peptidase M20D, amidohydrolase (NCBI), at 2,108,933 to 2,110,099
_A1813
Position (kb)
2107
2108
2109 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2106.303 kb on - strand, within Rru_A1809 at 2106.331 kb on + strand, within Rru_A1809 at 2106.331 kb on + strand, within Rru_A1809 at 2106.331 kb on + strand, within Rru_A1809 at 2106.332 kb on - strand, within Rru_A1809 at 2106.332 kb on - strand, within Rru_A1809 at 2106.452 kb on - strand, within Rru_A1809 at 2106.567 kb on - strand at 2106.567 kb on - strand at 2106.567 kb on - strand at 2106.567 kb on - strand at 2106.575 kb on + strand at 2106.767 kb on + strand, within Rru_A1810 at 2106.768 kb on - strand, within Rru_A1810 at 2106.768 kb on - strand, within Rru_A1810 at 2106.852 kb on + strand, within Rru_A1810 at 2106.852 kb on + strand, within Rru_A1810 at 2106.853 kb on - strand, within Rru_A1810 at 2106.853 kb on - strand, within Rru_A1810 at 2106.966 kb on + strand at 2106.966 kb on + strand at 2106.966 kb on + strand at 2106.967 kb on - strand at 2106.967 kb on - strand at 2106.967 kb on - strand at 2107.129 kb on + strand at 2107.130 kb on - strand at 2107.346 kb on - strand, within Rru_A1811 at 2107.405 kb on - strand, within Rru_A1811 at 2107.406 kb on + strand, within Rru_A1811 at 2107.407 kb on - strand, within Rru_A1811 at 2107.419 kb on - strand, within Rru_A1811 at 2107.427 kb on + strand, within Rru_A1811 at 2107.427 kb on + strand, within Rru_A1811 at 2107.427 kb on + strand, within Rru_A1811 at 2107.428 kb on - strand, within Rru_A1811 at 2107.428 kb on - strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.499 kb on + strand, within Rru_A1811 at 2107.500 kb on - strand, within Rru_A1811 at 2107.504 kb on + strand, within Rru_A1811 at 2107.504 kb on + strand, within Rru_A1811 at 2107.504 kb on + strand, within Rru_A1811 at 2107.504 kb on + strand, within Rru_A1811 at 2107.504 kb on + strand, within Rru_A1811 at 2107.504 kb on + strand, within Rru_A1811 at 2107.505 kb on - strand, within Rru_A1811 at 2107.505 kb on - strand, within Rru_A1811 at 2107.505 kb on - strand, within Rru_A1811 at 2107.686 kb on + strand, within Rru_A1811 at 2107.687 kb on - strand, within Rru_A1811 at 2107.809 kb on + strand, within Rru_A1811 at 2107.929 kb on + strand at 2107.929 kb on + strand at 2107.929 kb on + strand at 2107.929 kb on + strand at 2107.930 kb on - strand at 2107.940 kb on + strand at 2107.948 kb on - strand at 2107.948 kb on - strand at 2107.948 kb on - strand at 2108.225 kb on - strand, within Rru_A1812 at 2108.225 kb on - strand, within Rru_A1812 at 2108.260 kb on + strand, within Rru_A1812 at 2108.260 kb on + strand, within Rru_A1812 at 2108.260 kb on + strand, within Rru_A1812 at 2108.261 kb on - strand, within Rru_A1812 at 2108.261 kb on - strand, within Rru_A1812 at 2108.261 kb on - strand, within Rru_A1812 at 2108.261 kb on - strand, within Rru_A1812 at 2108.261 kb on - strand, within Rru_A1812 at 2108.271 kb on - strand, within Rru_A1812 at 2108.272 kb on + strand, within Rru_A1812 at 2108.273 kb on - strand, within Rru_A1812 at 2108.273 kb on - strand, within Rru_A1812 at 2108.273 kb on - strand, within Rru_A1812 at 2108.481 kb on + strand, within Rru_A1812 at 2108.482 kb on - strand, within Rru_A1812 at 2108.482 kb on - strand, within Rru_A1812 at 2108.482 kb on - strand, within Rru_A1812 at 2108.720 kb on + strand at 2108.720 kb on + strand at 2108.721 kb on - strand at 2108.722 kb on + strand at 2108.722 kb on + strand at 2108.722 kb on + strand at 2108.723 kb on - strand at 2108.723 kb on - strand at 2108.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 2,106,303 - Rru_A1809 0.59 -1.2 2,106,331 + Rru_A1809 0.64 -1.5 2,106,331 + Rru_A1809 0.64 +0.8 2,106,331 + Rru_A1809 0.64 -0.2 2,106,332 - Rru_A1809 0.64 -0.2 2,106,332 - Rru_A1809 0.64 -0.1 2,106,452 - Rru_A1809 0.86 -0.6 2,106,567 - -0.0 2,106,567 - -0.7 2,106,567 - -0.9 2,106,567 - -0.3 2,106,575 + -0.6 2,106,767 + Rru_A1810 0.54 -0.1 2,106,768 - Rru_A1810 0.54 -0.1 2,106,768 - Rru_A1810 0.54 -0.2 2,106,852 + Rru_A1810 0.74 +0.5 2,106,852 + Rru_A1810 0.74 -0.4 2,106,853 - Rru_A1810 0.74 +0.1 2,106,853 - Rru_A1810 0.74 +0.8 2,106,966 + -0.6 2,106,966 + +0.0 2,106,966 + -0.1 2,106,967 - -0.4 2,106,967 - -2.4 2,106,967 - +0.1 2,107,129 + -0.4 2,107,130 - -1.2 2,107,346 - Rru_A1811 0.22 +0.1 2,107,405 - Rru_A1811 0.29 -0.4 2,107,406 + Rru_A1811 0.29 -0.0 2,107,407 - Rru_A1811 0.29 +0.8 2,107,419 - Rru_A1811 0.30 -1.0 2,107,427 + Rru_A1811 0.31 -1.0 2,107,427 + Rru_A1811 0.31 -0.7 2,107,427 + Rru_A1811 0.31 -0.9 2,107,428 - Rru_A1811 0.31 -0.4 2,107,428 - Rru_A1811 0.31 -0.1 2,107,499 + Rru_A1811 0.40 -0.4 2,107,499 + Rru_A1811 0.40 -1.2 2,107,499 + Rru_A1811 0.40 +0.8 2,107,499 + Rru_A1811 0.40 -0.5 2,107,499 + Rru_A1811 0.40 -0.4 2,107,499 + Rru_A1811 0.40 +0.8 2,107,499 + Rru_A1811 0.40 -3.0 2,107,500 - Rru_A1811 0.40 +0.8 2,107,504 + Rru_A1811 0.40 -0.2 2,107,504 + Rru_A1811 0.40 +0.5 2,107,504 + Rru_A1811 0.40 -0.4 2,107,504 + Rru_A1811 0.40 -0.5 2,107,504 + Rru_A1811 0.40 -0.8 2,107,504 + Rru_A1811 0.40 -2.0 2,107,505 - Rru_A1811 0.40 -1.1 2,107,505 - Rru_A1811 0.40 +1.1 2,107,505 - Rru_A1811 0.40 +0.3 2,107,686 + Rru_A1811 0.62 -0.7 2,107,687 - Rru_A1811 0.62 -0.6 2,107,809 + Rru_A1811 0.76 -0.8 2,107,929 + -0.8 2,107,929 + -0.4 2,107,929 + -0.9 2,107,929 + -0.4 2,107,930 - -0.3 2,107,940 + -0.3 2,107,948 - -0.5 2,107,948 - -0.0 2,107,948 - +0.3 2,108,225 - Rru_A1812 0.29 -0.8 2,108,225 - Rru_A1812 0.29 -0.3 2,108,260 + Rru_A1812 0.33 -0.2 2,108,260 + Rru_A1812 0.33 +0.1 2,108,260 + Rru_A1812 0.33 +1.4 2,108,261 - Rru_A1812 0.33 -0.6 2,108,261 - Rru_A1812 0.33 -0.3 2,108,261 - Rru_A1812 0.33 -0.4 2,108,261 - Rru_A1812 0.33 +0.5 2,108,261 - Rru_A1812 0.33 -0.2 2,108,271 - Rru_A1812 0.35 +0.8 2,108,272 + Rru_A1812 0.35 -0.3 2,108,273 - Rru_A1812 0.35 -0.2 2,108,273 - Rru_A1812 0.35 +0.8 2,108,273 - Rru_A1812 0.35 -0.4 2,108,481 + Rru_A1812 0.62 -0.8 2,108,482 - Rru_A1812 0.63 +0.1 2,108,482 - Rru_A1812 0.63 +0.7 2,108,482 - Rru_A1812 0.63 +0.8 2,108,720 + +0.0 2,108,720 + +0.2 2,108,721 - +0.8 2,108,722 + -0.8 2,108,722 + -0.5 2,108,722 + +0.2 2,108,723 - -0.3 2,108,723 - -0.2 2,108,913 + +0.1
Or see this region's nucleotide sequence