Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1590 and Rru_A1591 are separated by 48 nucleotides Rru_A1591 and Rru_A1592 overlap by 4 nucleotides Rru_A1592 and Rru_A1593 are separated by 183 nucleotides
Rru_A1590: Rru_A1590 - Di-trans-poly-cis-decaprenylcistransferase (NCBI), at 1,873,813 to 1,874,547
_A1590
Rru_A1591: Rru_A1591 - Phosphatidate cytidylyltransferase (NCBI), at 1,874,596 to 1,875,402
_A1591
Rru_A1592: Rru_A1592 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase (NCBI), at 1,875,399 to 1,876,601
_A1592
Rru_A1593: Rru_A1593 - Peptidase M50, putative membrane-associated zinc metallopeptidase (NCBI), at 1,876,785 to 1,877,888
_A1593
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.545 kb on + strand at 1874.545 kb on + strand at 1874.545 kb on + strand at 1874.546 kb on - strand at 1874.546 kb on - strand at 1876.627 kb on + strand at 1876.628 kb on - strand at 1876.802 kb on + strand at 1876.990 kb on - strand, within Rru_A1593 at 1877.040 kb on - strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.170 kb on - strand, within Rru_A1593 at 1877.170 kb on - strand, within Rru_A1593 at 1877.171 kb on + strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.291 kb on - strand, within Rru_A1593 at 1877.291 kb on - strand, within Rru_A1593 at 1877.291 kb on - strand, within Rru_A1593 at 1877.349 kb on - strand, within Rru_A1593 at 1877.349 kb on - strand, within Rru_A1593 at 1877.355 kb on - strand, within Rru_A1593 at 1877.429 kb on + strand, within Rru_A1593 at 1877.429 kb on + strand, within Rru_A1593 at 1877.430 kb on - strand, within Rru_A1593 at 1877.430 kb on - strand, within Rru_A1593 at 1877.559 kb on + strand, within Rru_A1593 at 1877.559 kb on + strand, within Rru_A1593 at 1877.560 kb on - strand, within Rru_A1593
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 1,874,543 + -0.9 1,874,543 + -0.1 1,874,545 + -0.4 1,874,545 + -0.0 1,874,545 + -0.5 1,874,546 - +1.1 1,874,546 - +0.4 1,876,627 + -0.0 1,876,628 - +0.0 1,876,802 + -1.4 1,876,990 - Rru_A1593 0.19 -1.3 1,877,040 - Rru_A1593 0.23 -1.1 1,877,169 + Rru_A1593 0.35 -1.8 1,877,169 + Rru_A1593 0.35 -1.4 1,877,169 + Rru_A1593 0.35 -2.4 1,877,169 + Rru_A1593 0.35 +0.8 1,877,169 + Rru_A1593 0.35 -1.1 1,877,169 + Rru_A1593 0.35 -0.9 1,877,170 - Rru_A1593 0.35 -0.5 1,877,170 - Rru_A1593 0.35 -1.3 1,877,171 + Rru_A1593 0.35 -1.0 1,877,172 - Rru_A1593 0.35 +0.8 1,877,172 - Rru_A1593 0.35 -1.2 1,877,172 - Rru_A1593 0.35 -1.2 1,877,172 - Rru_A1593 0.35 -1.3 1,877,291 - Rru_A1593 0.46 -0.8 1,877,291 - Rru_A1593 0.46 +0.8 1,877,291 - Rru_A1593 0.46 +0.8 1,877,349 - Rru_A1593 0.51 +0.8 1,877,349 - Rru_A1593 0.51 -1.9 1,877,355 - Rru_A1593 0.52 -2.8 1,877,429 + Rru_A1593 0.58 +0.8 1,877,429 + Rru_A1593 0.58 -1.7 1,877,430 - Rru_A1593 0.58 -1.7 1,877,430 - Rru_A1593 0.58 -1.0 1,877,559 + Rru_A1593 0.70 -0.9 1,877,559 + Rru_A1593 0.70 +0.8 1,877,560 - Rru_A1593 0.70 -2.2
Or see this region's nucleotide sequence