Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0931 and Rru_A0932 are separated by 293 nucleotides Rru_A0932 and Rru_A0933 are separated by 159 nucleotides
Rru_A0931: Rru_A0931 - Aldehyde dehydrogenase (NAD+) (NCBI), at 1,107,226 to 1,108,746
_A0931
Rru_A0932: Rru_A0932 - Sigma54 Specific Transcriptional Regulator containing GAF, and Fis DNA-binding domains (NCBI), at 1,109,040 to 1,111,001
_A0932
Rru_A0933: Rru_A0933 - DegT/DnrJ/EryC1/StrS aminotransferase (NCBI), at 1,111,161 to 1,112,291
_A0933
Position (kb)
1109
1110
1111
1112 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1108.301 kb on + strand, within Rru_A0931 at 1108.448 kb on + strand, within Rru_A0931 at 1108.448 kb on + strand, within Rru_A0931 at 1108.643 kb on + strand at 1108.644 kb on - strand at 1108.644 kb on - strand at 1108.686 kb on + strand at 1108.691 kb on + strand at 1108.692 kb on - strand at 1108.692 kb on - strand at 1108.740 kb on - strand at 1108.965 kb on + strand at 1108.965 kb on + strand at 1108.974 kb on - strand at 1108.974 kb on - strand at 1109.287 kb on - strand, within Rru_A0932 at 1109.287 kb on - strand, within Rru_A0932 at 1109.287 kb on - strand, within Rru_A0932 at 1109.585 kb on + strand, within Rru_A0932 at 1109.586 kb on - strand, within Rru_A0932 at 1109.586 kb on - strand, within Rru_A0932 at 1109.586 kb on - strand, within Rru_A0932 at 1109.586 kb on - strand, within Rru_A0932 at 1109.648 kb on + strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.649 kb on - strand, within Rru_A0932 at 1109.692 kb on - strand, within Rru_A0932 at 1109.692 kb on - strand, within Rru_A0932 at 1110.246 kb on + strand, within Rru_A0932 at 1110.247 kb on - strand, within Rru_A0932 at 1110.344 kb on + strand, within Rru_A0932 at 1110.507 kb on + strand, within Rru_A0932 at 1110.508 kb on - strand, within Rru_A0932 at 1110.508 kb on - strand, within Rru_A0932 at 1110.508 kb on - strand, within Rru_A0932 at 1110.508 kb on - strand, within Rru_A0932 at 1110.511 kb on - strand, within Rru_A0932 at 1110.920 kb on + strand at 1110.920 kb on + strand at 1110.921 kb on - strand at 1110.921 kb on - strand at 1110.921 kb on - strand at 1110.927 kb on + strand at 1110.927 kb on + strand at 1110.927 kb on + strand at 1110.928 kb on - strand at 1111.116 kb on + strand at 1111.125 kb on + strand at 1111.251 kb on + strand at 1111.251 kb on + strand at 1111.252 kb on - strand at 1111.252 kb on - strand at 1111.310 kb on - strand, within Rru_A0933 at 1111.410 kb on - strand, within Rru_A0933 at 1111.485 kb on + strand, within Rru_A0933 at 1111.485 kb on + strand, within Rru_A0933 at 1111.485 kb on + strand, within Rru_A0933 at 1111.485 kb on + strand, within Rru_A0933 at 1111.485 kb on + strand, within Rru_A0933 at 1111.486 kb on - strand, within Rru_A0933 at 1111.486 kb on - strand, within Rru_A0933 at 1111.486 kb on - strand, within Rru_A0933 at 1111.486 kb on - strand, within Rru_A0933 at 1111.486 kb on - strand, within Rru_A0933 at 1111.581 kb on + strand, within Rru_A0933 at 1111.582 kb on - strand, within Rru_A0933 at 1111.821 kb on + strand, within Rru_A0933 at 1111.821 kb on + strand, within Rru_A0933 at 1111.822 kb on - strand, within Rru_A0933 at 1111.839 kb on + strand, within Rru_A0933 at 1111.839 kb on + strand, within Rru_A0933 at 1111.840 kb on - strand, within Rru_A0933 at 1111.840 kb on - strand, within Rru_A0933 at 1111.862 kb on + strand, within Rru_A0933 at 1111.862 kb on + strand, within Rru_A0933 at 1111.862 kb on + strand, within Rru_A0933 at 1111.863 kb on - strand, within Rru_A0933 at 1111.960 kb on - strand, within Rru_A0933
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 1,108,301 + Rru_A0931 0.71 -0.2 1,108,448 + Rru_A0931 0.80 -1.0 1,108,448 + Rru_A0931 0.80 -1.0 1,108,643 + -0.9 1,108,644 - -1.1 1,108,644 - +1.2 1,108,686 + -2.3 1,108,691 + -1.0 1,108,692 - -0.1 1,108,692 - -1.7 1,108,740 - -1.2 1,108,965 + +1.1 1,108,965 + +0.6 1,108,974 - -0.5 1,108,974 - -0.8 1,109,287 - Rru_A0932 0.13 -1.4 1,109,287 - Rru_A0932 0.13 -0.3 1,109,287 - Rru_A0932 0.13 -0.8 1,109,585 + Rru_A0932 0.28 -1.2 1,109,586 - Rru_A0932 0.28 -0.9 1,109,586 - Rru_A0932 0.28 +0.7 1,109,586 - Rru_A0932 0.28 -1.3 1,109,586 - Rru_A0932 0.28 -1.1 1,109,648 + Rru_A0932 0.31 -1.1 1,109,649 - Rru_A0932 0.31 -1.7 1,109,649 - Rru_A0932 0.31 -1.5 1,109,649 - Rru_A0932 0.31 -1.2 1,109,649 - Rru_A0932 0.31 +0.2 1,109,649 - Rru_A0932 0.31 +0.0 1,109,649 - Rru_A0932 0.31 -0.9 1,109,649 - Rru_A0932 0.31 -1.8 1,109,692 - Rru_A0932 0.33 -1.1 1,109,692 - Rru_A0932 0.33 -1.2 1,110,246 + Rru_A0932 0.61 -0.7 1,110,247 - Rru_A0932 0.62 -0.8 1,110,344 + Rru_A0932 0.66 -1.2 1,110,507 + Rru_A0932 0.75 -1.0 1,110,508 - Rru_A0932 0.75 -1.0 1,110,508 - Rru_A0932 0.75 -1.3 1,110,508 - Rru_A0932 0.75 -1.8 1,110,508 - Rru_A0932 0.75 -2.3 1,110,511 - Rru_A0932 0.75 -1.4 1,110,920 + -0.8 1,110,920 + -1.2 1,110,921 - -0.9 1,110,921 - -0.7 1,110,921 - -1.3 1,110,927 + -2.0 1,110,927 + -0.7 1,110,927 + -2.8 1,110,928 - -0.9 1,111,116 + +0.6 1,111,125 + -0.0 1,111,251 + -0.5 1,111,251 + -0.2 1,111,252 - -0.8 1,111,252 - +0.1 1,111,310 - Rru_A0933 0.13 -1.4 1,111,410 - Rru_A0933 0.22 -0.9 1,111,485 + Rru_A0933 0.29 +0.1 1,111,485 + Rru_A0933 0.29 +0.8 1,111,485 + Rru_A0933 0.29 -0.0 1,111,485 + Rru_A0933 0.29 -0.3 1,111,485 + Rru_A0933 0.29 +0.3 1,111,486 - Rru_A0933 0.29 -0.4 1,111,486 - Rru_A0933 0.29 +0.1 1,111,486 - Rru_A0933 0.29 -0.2 1,111,486 - Rru_A0933 0.29 -0.7 1,111,486 - Rru_A0933 0.29 -0.2 1,111,581 + Rru_A0933 0.37 -0.3 1,111,582 - Rru_A0933 0.37 -0.5 1,111,821 + Rru_A0933 0.58 -0.2 1,111,821 + Rru_A0933 0.58 -0.8 1,111,822 - Rru_A0933 0.58 -0.7 1,111,839 + Rru_A0933 0.60 +0.8 1,111,839 + Rru_A0933 0.60 -0.2 1,111,840 - Rru_A0933 0.60 -0.5 1,111,840 - Rru_A0933 0.60 -0.4 1,111,862 + Rru_A0933 0.62 -0.1 1,111,862 + Rru_A0933 0.62 -0.4 1,111,862 + Rru_A0933 0.62 -0.0 1,111,863 - Rru_A0933 0.62 -0.1 1,111,960 - Rru_A0933 0.71 -0.2
Or see this region's nucleotide sequence