Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0453 and Rru_A0454 are separated by 247 nucleotides Rru_A0454 and Rru_A0455 are separated by 37 nucleotides Rru_A0455 and Rru_A0456 are separated by 10 nucleotides Rru_A0456 and Rru_A0457 are separated by 1 nucleotides
Rru_A0453: Rru_A0453 - HNH endonuclease (NCBI), at 530,734 to 534,255
_A0453
Rru_A0454: Rru_A0454 - Leucyl aminopeptidase (NCBI), at 534,503 to 536,005
_A0454
Rru_A0455: Rru_A0455 - NUDIX hydrolase (NCBI), at 536,043 to 536,558
_A0455
Rru_A0456: Rru_A0456 - Predicted transcriptional regulator (NCBI), at 536,569 to 536,856
_A0456
Rru_A0457: Rru_A0457 - Protein of unknown function DUF891 (NCBI), at 536,858 to 537,154
_A0457
Position (kb) 534
535
536
537 Strain fitness (log2 ratio) -2
-1
0
1 at 533.628 kb on + strand, within Rru_A0453 at 533.629 kb on - strand, within Rru_A0453 at 533.630 kb on + strand, within Rru_A0453 at 533.630 kb on + strand, within Rru_A0453 at 533.630 kb on + strand, within Rru_A0453 at 533.630 kb on + strand, within Rru_A0453 at 533.631 kb on - strand, within Rru_A0453 at 534.373 kb on + strand at 534.912 kb on - strand, within Rru_A0454 at 534.927 kb on - strand, within Rru_A0454 at 535.013 kb on + strand, within Rru_A0454 at 535.089 kb on - strand, within Rru_A0454 at 535.245 kb on - strand, within Rru_A0454 at 535.339 kb on + strand, within Rru_A0454 at 535.575 kb on - strand, within Rru_A0454 at 535.760 kb on + strand, within Rru_A0454 at 535.970 kb on + strand at 535.970 kb on + strand at 535.971 kb on - strand at 535.971 kb on - strand at 535.971 kb on - strand at 536.003 kb on + strand at 536.004 kb on - strand at 536.004 kb on - strand at 536.079 kb on - strand at 536.193 kb on + strand, within Rru_A0455 at 536.193 kb on + strand, within Rru_A0455 at 536.193 kb on + strand, within Rru_A0455 at 536.193 kb on + strand, within Rru_A0455 at 536.194 kb on - strand, within Rru_A0455 at 536.194 kb on - strand, within Rru_A0455 at 536.194 kb on - strand, within Rru_A0455 at 536.194 kb on - strand, within Rru_A0455 at 536.194 kb on - strand, within Rru_A0455 at 536.194 kb on - strand, within Rru_A0455 at 536.316 kb on + strand, within Rru_A0455 at 536.316 kb on + strand, within Rru_A0455 at 536.316 kb on + strand, within Rru_A0455 at 536.316 kb on + strand, within Rru_A0455 at 536.317 kb on - strand, within Rru_A0455 at 536.317 kb on - strand, within Rru_A0455 at 536.317 kb on - strand, within Rru_A0455 at 536.318 kb on + strand, within Rru_A0455 at 536.751 kb on + strand, within Rru_A0456 at 536.751 kb on + strand, within Rru_A0456 at 536.751 kb on + strand, within Rru_A0456 at 536.752 kb on - strand, within Rru_A0456 at 536.752 kb on - strand, within Rru_A0456 at 536.753 kb on + strand, within Rru_A0456 at 536.753 kb on + strand, within Rru_A0456 at 536.753 kb on + strand, within Rru_A0456 at 536.753 kb on + strand, within Rru_A0456 at 536.754 kb on - strand, within Rru_A0456 at 536.754 kb on - strand, within Rru_A0456 at 536.834 kb on + strand at 536.834 kb on + strand at 536.835 kb on - strand at 536.835 kb on - strand at 536.835 kb on - strand at 536.992 kb on - strand, within Rru_A0457
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 533,628 + Rru_A0453 0.82 +0.1 533,629 - Rru_A0453 0.82 +0.1 533,630 + Rru_A0453 0.82 +0.2 533,630 + Rru_A0453 0.82 -0.2 533,630 + Rru_A0453 0.82 -0.3 533,630 + Rru_A0453 0.82 +0.4 533,631 - Rru_A0453 0.82 -1.1 534,373 + -0.5 534,912 - Rru_A0454 0.27 -1.7 534,927 - Rru_A0454 0.28 -1.7 535,013 + Rru_A0454 0.34 -0.3 535,089 - Rru_A0454 0.39 -1.1 535,245 - Rru_A0454 0.49 -2.2 535,339 + Rru_A0454 0.56 +0.2 535,575 - Rru_A0454 0.71 -1.1 535,760 + Rru_A0454 0.84 +0.1 535,970 + -1.1 535,970 + -0.8 535,971 - -2.2 535,971 - -0.3 535,971 - -0.1 536,003 + -0.1 536,004 - +0.9 536,004 - +0.2 536,079 - +0.4 536,193 + Rru_A0455 0.29 +0.5 536,193 + Rru_A0455 0.29 -0.5 536,193 + Rru_A0455 0.29 +1.4 536,193 + Rru_A0455 0.29 -0.3 536,194 - Rru_A0455 0.29 +0.9 536,194 - Rru_A0455 0.29 -0.1 536,194 - Rru_A0455 0.29 +0.5 536,194 - Rru_A0455 0.29 +0.3 536,194 - Rru_A0455 0.29 +0.2 536,194 - Rru_A0455 0.29 +0.0 536,316 + Rru_A0455 0.53 -0.1 536,316 + Rru_A0455 0.53 -0.1 536,316 + Rru_A0455 0.53 -0.1 536,316 + Rru_A0455 0.53 -0.1 536,317 - Rru_A0455 0.53 +0.9 536,317 - Rru_A0455 0.53 +0.5 536,317 - Rru_A0455 0.53 +0.1 536,318 + Rru_A0455 0.53 -0.7 536,751 + Rru_A0456 0.63 +0.9 536,751 + Rru_A0456 0.63 +0.1 536,751 + Rru_A0456 0.63 +0.4 536,752 - Rru_A0456 0.64 -0.7 536,752 - Rru_A0456 0.64 -0.5 536,753 + Rru_A0456 0.64 -1.5 536,753 + Rru_A0456 0.64 -0.2 536,753 + Rru_A0456 0.64 +0.1 536,753 + Rru_A0456 0.64 -0.1 536,754 - Rru_A0456 0.64 +0.7 536,754 - Rru_A0456 0.64 +1.6 536,834 + -1.5 536,834 + -0.6 536,835 - +0.0 536,835 - +0.0 536,835 - -0.6 536,992 - Rru_A0457 0.45 -0.5
Or see this region's nucleotide sequence