Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0152 and Rru_A0153 are separated by 973 nucleotides Rru_A0153 and Rru_A0154 are separated by 5 nucleotides
Rru_A0152: Rru_A0152 - hypothetical protein (NCBI), at 176,479 to 176,895
_A0152
Rru_A0153: Rru_A0153 - Endonuclease III/Nth (NCBI), at 177,869 to 178,582
_A0153
Rru_A0154: Rru_A0154 - Dihydrodipicolinate reductase (NCBI), at 178,588 to 179,385
_A0154
Position (kb) 177
178
179 Strain fitness (log2 ratio) -2
-1
0
1 at 176.894 kb on - strand at 177.003 kb on + strand at 177.089 kb on + strand at 177.104 kb on - strand at 177.108 kb on - strand at 177.154 kb on + strand at 177.264 kb on - strand at 177.265 kb on + strand at 177.367 kb on - strand at 177.404 kb on - strand at 177.448 kb on - strand at 177.448 kb on - strand at 177.478 kb on - strand at 177.737 kb on + strand at 177.737 kb on + strand at 177.755 kb on + strand at 177.757 kb on + strand at 177.843 kb on + strand at 177.850 kb on + strand at 177.850 kb on + strand at 177.851 kb on - strand at 177.872 kb on + strand at 177.873 kb on - strand at 178.087 kb on + strand, within Rru_A0153 at 178.087 kb on + strand, within Rru_A0153 at 178.087 kb on + strand, within Rru_A0153 at 178.088 kb on - strand, within Rru_A0153 at 178.284 kb on + strand, within Rru_A0153 at 178.284 kb on + strand, within Rru_A0153 at 178.284 kb on + strand, within Rru_A0153 at 178.285 kb on - strand, within Rru_A0153 at 178.285 kb on - strand, within Rru_A0153 at 178.285 kb on - strand, within Rru_A0153 at 178.431 kb on + strand, within Rru_A0153 at 178.432 kb on - strand, within Rru_A0153 at 179.488 kb on + strand at 179.489 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 176,894 - -0.6 177,003 + -1.8 177,089 + +0.7 177,104 - +0.7 177,108 - -0.1 177,154 + -0.3 177,264 - +0.4 177,265 + -0.2 177,367 - +0.9 177,404 - -0.6 177,448 - +0.3 177,448 - -0.2 177,478 - +0.0 177,737 + +0.8 177,737 + -0.2 177,755 + -0.2 177,757 + -0.5 177,843 + +0.1 177,850 + +0.9 177,850 + -0.4 177,851 - -0.1 177,872 + -0.9 177,873 - -0.3 178,087 + Rru_A0153 0.31 -0.7 178,087 + Rru_A0153 0.31 -1.4 178,087 + Rru_A0153 0.31 -1.3 178,088 - Rru_A0153 0.31 -0.4 178,284 + Rru_A0153 0.58 -0.2 178,284 + Rru_A0153 0.58 -0.6 178,284 + Rru_A0153 0.58 -0.6 178,285 - Rru_A0153 0.58 -0.7 178,285 - Rru_A0153 0.58 -1.1 178,285 - Rru_A0153 0.58 -1.4 178,431 + Rru_A0153 0.79 -0.4 178,432 - Rru_A0153 0.79 +0.9 179,488 + +0.4 179,489 - -0.3
Or see this region's nucleotide sequence