Experiment: Low light with ammonium chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0109 and Rru_A0110 are separated by 11 nucleotides Rru_A0110 and Rru_A0111 overlap by 4 nucleotides Rru_A0111 and Rru_A0112 overlap by 4 nucleotides
Rru_A0109: Rru_A0109 - type II and III secretion system protein (NCBI), at 127,711 to 129,354
_A0109
Rru_A0110: Rru_A0110 - hypothetical protein (NCBI), at 129,366 to 130,115
_A0110
Rru_A0111: Rru_A0111 - hypothetical protein (NCBI), at 130,112 to 130,702
_A0111
Rru_A0112: Rru_A0112 - hypothetical protein (NCBI), at 130,699 to 131,421
_A0112
Position (kb)
129
130
131 Strain fitness (log2 ratio)
-1
0
1
2 at 128.720 kb on + strand, within Rru_A0109 at 128.732 kb on + strand, within Rru_A0109 at 128.732 kb on + strand, within Rru_A0109 at 128.732 kb on + strand, within Rru_A0109 at 128.732 kb on + strand, within Rru_A0109 at 128.733 kb on - strand, within Rru_A0109 at 128.733 kb on - strand, within Rru_A0109 at 128.836 kb on + strand, within Rru_A0109 at 128.836 kb on + strand, within Rru_A0109 at 128.836 kb on + strand, within Rru_A0109 at 128.837 kb on - strand, within Rru_A0109 at 128.837 kb on - strand, within Rru_A0109 at 128.837 kb on - strand, within Rru_A0109 at 128.890 kb on + strand, within Rru_A0109 at 128.890 kb on + strand, within Rru_A0109 at 128.890 kb on + strand, within Rru_A0109 at 128.891 kb on - strand, within Rru_A0109 at 128.891 kb on - strand, within Rru_A0109 at 128.891 kb on - strand, within Rru_A0109 at 128.907 kb on + strand, within Rru_A0109 at 128.907 kb on + strand, within Rru_A0109 at 128.907 kb on + strand, within Rru_A0109 at 129.060 kb on + strand, within Rru_A0109 at 129.061 kb on - strand, within Rru_A0109 at 129.061 kb on - strand, within Rru_A0109 at 129.061 kb on - strand, within Rru_A0109 at 129.288 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.322 kb on + strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.323 kb on - strand at 129.352 kb on + strand at 129.352 kb on + strand at 129.352 kb on + strand at 129.684 kb on - strand, within Rru_A0110 at 129.855 kb on + strand, within Rru_A0110 at 129.856 kb on - strand, within Rru_A0110 at 129.856 kb on - strand, within Rru_A0110 at 129.856 kb on - strand, within Rru_A0110
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with ammonium chloride remove 128,720 + Rru_A0109 0.61 +0.1 128,732 + Rru_A0109 0.62 -0.4 128,732 + Rru_A0109 0.62 -1.2 128,732 + Rru_A0109 0.62 -0.3 128,732 + Rru_A0109 0.62 -1.4 128,733 - Rru_A0109 0.62 -0.5 128,733 - Rru_A0109 0.62 -1.3 128,836 + Rru_A0109 0.68 -0.1 128,836 + Rru_A0109 0.68 +0.4 128,836 + Rru_A0109 0.68 -0.1 128,837 - Rru_A0109 0.68 +0.9 128,837 - Rru_A0109 0.68 -0.5 128,837 - Rru_A0109 0.68 +0.1 128,890 + Rru_A0109 0.72 -0.1 128,890 + Rru_A0109 0.72 +0.8 128,890 + Rru_A0109 0.72 -0.1 128,891 - Rru_A0109 0.72 -0.4 128,891 - Rru_A0109 0.72 +0.9 128,891 - Rru_A0109 0.72 +0.2 128,907 + Rru_A0109 0.73 +0.2 128,907 + Rru_A0109 0.73 -0.2 128,907 + Rru_A0109 0.73 -0.7 129,060 + Rru_A0109 0.82 +0.1 129,061 - Rru_A0109 0.82 +0.9 129,061 - Rru_A0109 0.82 +0.3 129,061 - Rru_A0109 0.82 +0.3 129,288 + -0.4 129,322 + -0.3 129,322 + -0.9 129,322 + -0.6 129,322 + +0.9 129,322 + -0.5 129,322 + +2.0 129,322 + -0.9 129,322 + -0.2 129,323 - -0.3 129,323 - -0.6 129,323 - +0.1 129,323 - +0.9 129,323 - -1.1 129,323 - -0.5 129,323 - +0.7 129,323 - -0.5 129,352 + -0.1 129,352 + -0.0 129,352 + +0.3 129,684 - Rru_A0110 0.42 -0.7 129,855 + Rru_A0110 0.65 +0.0 129,856 - Rru_A0110 0.65 -0.6 129,856 - Rru_A0110 0.65 -1.4 129,856 - Rru_A0110 0.65 +0.9
Or see this region's nucleotide sequence