Experiment: Chloride 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_192 and Pf6N2E2_193 are separated by 377 nucleotides Pf6N2E2_193 and Pf6N2E2_194 overlap by 36 nucleotides Pf6N2E2_194 and Pf6N2E2_195 overlap by 4 nucleotides
Pf6N2E2_192 - hypothetical protein, at 244,676 to 246,115
_192
Pf6N2E2_193 - hypothetical protein, at 246,493 to 246,870
_193
Pf6N2E2_194 - Alcohol dehydrogenase (EC 1.1.1.1), at 246,835 to 247,569
_194
Pf6N2E2_195 - hypothetical protein, at 247,566 to 249,515
_195
Position (kb) 246
247 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 245.591 kb on + strand, within Pf6N2E2_192 at 245.591 kb on + strand, within Pf6N2E2_192 at 245.591 kb on + strand, within Pf6N2E2_192 at 245.592 kb on - strand, within Pf6N2E2_192 at 245.592 kb on - strand, within Pf6N2E2_192 at 245.780 kb on - strand, within Pf6N2E2_192 at 245.833 kb on + strand, within Pf6N2E2_192 at 245.834 kb on - strand, within Pf6N2E2_192 at 245.861 kb on + strand, within Pf6N2E2_192 at 246.091 kb on - strand at 246.091 kb on - strand at 246.173 kb on + strand at 246.173 kb on + strand at 246.174 kb on - strand at 246.174 kb on - strand at 246.249 kb on - strand at 246.253 kb on + strand at 246.254 kb on - strand at 246.254 kb on - strand at 246.254 kb on - strand at 246.254 kb on - strand at 246.286 kb on + strand at 246.286 kb on + strand at 246.304 kb on + strand at 246.304 kb on + strand at 246.304 kb on + strand at 246.305 kb on - strand at 246.305 kb on - strand at 246.305 kb on - strand at 246.305 kb on - strand at 246.305 kb on - strand at 246.353 kb on - strand at 246.462 kb on + strand at 246.465 kb on + strand at 246.465 kb on + strand at 246.465 kb on + strand at 246.498 kb on + strand at 246.498 kb on + strand at 246.500 kb on + strand at 246.511 kb on + strand at 246.511 kb on + strand at 246.512 kb on - strand at 246.517 kb on - strand at 246.539 kb on + strand, within Pf6N2E2_193 at 246.539 kb on + strand, within Pf6N2E2_193 at 246.539 kb on + strand, within Pf6N2E2_193 at 246.544 kb on + strand, within Pf6N2E2_193 at 246.544 kb on + strand, within Pf6N2E2_193 at 246.772 kb on - strand, within Pf6N2E2_193 at 246.889 kb on + strand at 246.889 kb on + strand at 246.889 kb on + strand at 246.890 kb on - strand at 247.116 kb on + strand, within Pf6N2E2_194 at 247.117 kb on - strand, within Pf6N2E2_194 at 247.205 kb on + strand, within Pf6N2E2_194 at 247.215 kb on + strand, within Pf6N2E2_194 at 247.216 kb on - strand, within Pf6N2E2_194 at 247.376 kb on - strand, within Pf6N2E2_194 at 247.376 kb on - strand, within Pf6N2E2_194 at 247.376 kb on - strand, within Pf6N2E2_194 at 247.376 kb on - strand, within Pf6N2E2_194 at 247.645 kb on + strand at 247.652 kb on - strand at 247.652 kb on - strand at 247.751 kb on + strand at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.861 kb on + strand, within Pf6N2E2_195 at 247.862 kb on - strand, within Pf6N2E2_195 at 247.862 kb on - strand, within Pf6N2E2_195 at 247.862 kb on - strand, within Pf6N2E2_195 at 247.862 kb on - strand, within Pf6N2E2_195
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 0.5 M remove 245,591 + Pf6N2E2_192 0.64 -0.1 245,591 + Pf6N2E2_192 0.64 -0.9 245,591 + Pf6N2E2_192 0.64 +0.5 245,592 - Pf6N2E2_192 0.64 -2.9 245,592 - Pf6N2E2_192 0.64 +0.0 245,780 - Pf6N2E2_192 0.77 -1.0 245,833 + Pf6N2E2_192 0.80 -0.4 245,834 - Pf6N2E2_192 0.80 -1.3 245,861 + Pf6N2E2_192 0.82 +0.4 246,091 - +0.6 246,091 - -0.2 246,173 + -0.9 246,173 + +0.5 246,174 - +4.2 246,174 - -0.2 246,249 - -2.1 246,253 + -0.2 246,254 - +1.4 246,254 - +0.7 246,254 - +0.1 246,254 - -0.7 246,286 + -0.3 246,286 + -0.6 246,304 + +0.2 246,304 + +0.0 246,304 + -0.0 246,305 - +0.1 246,305 - -2.0 246,305 - +0.2 246,305 - +0.9 246,305 - +0.1 246,353 - +1.6 246,462 + +0.6 246,465 + +0.2 246,465 + +0.5 246,465 + -0.2 246,498 + -2.0 246,498 + +0.3 246,500 + -1.4 246,511 + +2.0 246,511 + -1.6 246,512 - -0.1 246,517 - -1.2 246,539 + Pf6N2E2_193 0.12 +0.3 246,539 + Pf6N2E2_193 0.12 -1.1 246,539 + Pf6N2E2_193 0.12 -1.2 246,544 + Pf6N2E2_193 0.13 -1.9 246,544 + Pf6N2E2_193 0.13 -1.6 246,772 - Pf6N2E2_193 0.74 -1.2 246,889 + +0.1 246,889 + +1.3 246,889 + -1.4 246,890 - +1.2 247,116 + Pf6N2E2_194 0.38 +0.6 247,117 - Pf6N2E2_194 0.38 +0.8 247,205 + Pf6N2E2_194 0.50 +0.7 247,215 + Pf6N2E2_194 0.52 +0.1 247,216 - Pf6N2E2_194 0.52 -1.3 247,376 - Pf6N2E2_194 0.74 +0.4 247,376 - Pf6N2E2_194 0.74 -0.3 247,376 - Pf6N2E2_194 0.74 -1.0 247,376 - Pf6N2E2_194 0.74 +0.2 247,645 + -0.6 247,652 - +0.5 247,652 - +1.9 247,751 + -0.9 247,861 + Pf6N2E2_195 0.15 -1.2 247,861 + Pf6N2E2_195 0.15 +0.1 247,861 + Pf6N2E2_195 0.15 +0.0 247,861 + Pf6N2E2_195 0.15 +1.6 247,861 + Pf6N2E2_195 0.15 +0.2 247,861 + Pf6N2E2_195 0.15 +0.0 247,861 + Pf6N2E2_195 0.15 -0.2 247,861 + Pf6N2E2_195 0.15 -0.4 247,861 + Pf6N2E2_195 0.15 +0.5 247,861 + Pf6N2E2_195 0.15 -0.6 247,862 - Pf6N2E2_195 0.15 +0.3 247,862 - Pf6N2E2_195 0.15 +0.2 247,862 - Pf6N2E2_195 0.15 +0.0 247,862 - Pf6N2E2_195 0.15 +0.4
Or see this region's nucleotide sequence