Experiment: Chloride 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1225 and Pf6N2E2_1226 overlap by 4 nucleotides Pf6N2E2_1226 and Pf6N2E2_1227 are separated by 220 nucleotides Pf6N2E2_1227 and Pf6N2E2_1228 are separated by 128 nucleotides
Pf6N2E2_1225 - DinG family ATP-dependent helicase CPE1197, at 1,432,159 to 1,434,471
_1225
Pf6N2E2_1226 - DNA damage response nuclease (from data) , at 1,434,468 to 1,436,138
_1226
Pf6N2E2_1227 - FIG00955097: hypothetical protein, at 1,436,359 to 1,436,583
_1227
Pf6N2E2_1228 - Lipase (EC 3.1.1.3), at 1,436,712 to 1,438,565
_1228
Position (kb)
1434
1435
1436
1437 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.477 kb on + strand, within Pf6N2E2_1225 at 1433.481 kb on - strand, within Pf6N2E2_1225 at 1433.481 kb on - strand, within Pf6N2E2_1225 at 1433.579 kb on + strand, within Pf6N2E2_1225 at 1433.580 kb on - strand, within Pf6N2E2_1225 at 1433.701 kb on + strand, within Pf6N2E2_1225 at 1433.702 kb on - strand, within Pf6N2E2_1225 at 1433.702 kb on - strand, within Pf6N2E2_1225 at 1433.764 kb on - strand, within Pf6N2E2_1225 at 1433.998 kb on + strand, within Pf6N2E2_1225 at 1433.999 kb on - strand, within Pf6N2E2_1225 at 1434.054 kb on + strand, within Pf6N2E2_1225 at 1434.122 kb on - strand, within Pf6N2E2_1225 at 1434.122 kb on - strand, within Pf6N2E2_1225 at 1434.514 kb on + strand at 1434.514 kb on + strand at 1434.514 kb on + strand at 1434.515 kb on - strand at 1434.515 kb on - strand at 1434.774 kb on + strand, within Pf6N2E2_1226 at 1434.852 kb on + strand, within Pf6N2E2_1226 at 1434.853 kb on - strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.018 kb on + strand, within Pf6N2E2_1226 at 1435.019 kb on - strand, within Pf6N2E2_1226 at 1435.019 kb on - strand, within Pf6N2E2_1226 at 1435.324 kb on + strand, within Pf6N2E2_1226 at 1435.324 kb on + strand, within Pf6N2E2_1226 at 1435.325 kb on - strand, within Pf6N2E2_1226 at 1435.570 kb on + strand, within Pf6N2E2_1226 at 1435.679 kb on - strand, within Pf6N2E2_1226 at 1435.679 kb on - strand, within Pf6N2E2_1226 at 1436.104 kb on + strand at 1436.104 kb on + strand at 1436.107 kb on + strand at 1436.107 kb on + strand at 1436.108 kb on - strand at 1436.108 kb on - strand at 1436.191 kb on + strand at 1436.229 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.231 kb on + strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.232 kb on - strand at 1436.235 kb on + strand at 1436.235 kb on + strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.236 kb on - strand at 1436.352 kb on + strand at 1436.461 kb on + strand, within Pf6N2E2_1227 at 1436.652 kb on + strand at 1436.652 kb on + strand at 1436.653 kb on - strand at 1436.658 kb on + strand at 1436.659 kb on - strand at 1436.665 kb on + strand at 1436.665 kb on + strand at 1436.666 kb on - strand at 1436.821 kb on + strand at 1436.821 kb on + strand at 1436.822 kb on - strand at 1436.830 kb on + strand at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.106 kb on + strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228 at 1437.107 kb on - strand, within Pf6N2E2_1228
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 0.5 M remove 1,433,477 + Pf6N2E2_1225 0.57 -0.8 1,433,477 + Pf6N2E2_1225 0.57 -1.1 1,433,477 + Pf6N2E2_1225 0.57 +1.1 1,433,481 - Pf6N2E2_1225 0.57 -1.1 1,433,481 - Pf6N2E2_1225 0.57 +0.5 1,433,579 + Pf6N2E2_1225 0.61 -1.3 1,433,580 - Pf6N2E2_1225 0.61 -0.1 1,433,701 + Pf6N2E2_1225 0.67 -0.1 1,433,702 - Pf6N2E2_1225 0.67 -0.2 1,433,702 - Pf6N2E2_1225 0.67 +0.2 1,433,764 - Pf6N2E2_1225 0.69 +0.1 1,433,998 + Pf6N2E2_1225 0.80 +0.2 1,433,999 - Pf6N2E2_1225 0.80 -1.6 1,434,054 + Pf6N2E2_1225 0.82 -0.2 1,434,122 - Pf6N2E2_1225 0.85 +0.3 1,434,122 - Pf6N2E2_1225 0.85 +1.5 1,434,514 + +0.4 1,434,514 + -0.5 1,434,514 + +0.5 1,434,515 - +0.0 1,434,515 - -0.4 1,434,774 + Pf6N2E2_1226 0.18 -0.7 1,434,852 + Pf6N2E2_1226 0.23 -1.3 1,434,853 - Pf6N2E2_1226 0.23 -2.3 1,435,018 + Pf6N2E2_1226 0.33 -1.5 1,435,018 + Pf6N2E2_1226 0.33 +0.7 1,435,018 + Pf6N2E2_1226 0.33 -0.6 1,435,018 + Pf6N2E2_1226 0.33 -2.0 1,435,018 + Pf6N2E2_1226 0.33 +0.1 1,435,019 - Pf6N2E2_1226 0.33 -0.8 1,435,019 - Pf6N2E2_1226 0.33 +1.8 1,435,324 + Pf6N2E2_1226 0.51 -0.9 1,435,324 + Pf6N2E2_1226 0.51 -2.1 1,435,325 - Pf6N2E2_1226 0.51 +1.5 1,435,570 + Pf6N2E2_1226 0.66 +1.7 1,435,679 - Pf6N2E2_1226 0.72 -1.8 1,435,679 - Pf6N2E2_1226 0.72 -0.1 1,436,104 + +2.5 1,436,104 + +0.1 1,436,107 + +1.5 1,436,107 + +0.1 1,436,108 - +0.1 1,436,108 - -0.5 1,436,191 + +0.5 1,436,229 + +0.9 1,436,231 + +1.6 1,436,231 + +0.5 1,436,231 + +0.3 1,436,232 - +0.2 1,436,232 - -0.8 1,436,232 - -0.4 1,436,235 + +0.5 1,436,235 + +0.2 1,436,236 - +1.7 1,436,236 - -0.6 1,436,236 - +1.6 1,436,352 + -0.8 1,436,461 + Pf6N2E2_1227 0.45 +0.8 1,436,652 + -0.5 1,436,652 + -0.8 1,436,653 - +0.5 1,436,658 + +2.1 1,436,659 - +1.4 1,436,665 + -0.7 1,436,665 + -1.1 1,436,666 - -0.9 1,436,821 + +0.5 1,436,821 + +2.0 1,436,822 - -1.3 1,436,830 + -1.9 1,437,106 + Pf6N2E2_1228 0.21 -0.7 1,437,106 + Pf6N2E2_1228 0.21 -0.1 1,437,106 + Pf6N2E2_1228 0.21 +1.0 1,437,107 - Pf6N2E2_1228 0.21 -0.8 1,437,107 - Pf6N2E2_1228 0.21 -0.2 1,437,107 - Pf6N2E2_1228 0.21 +0.1
Or see this region's nucleotide sequence