Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1177

Experiment: Chloride 0.5 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1175 and Pf6N2E2_1176 are separated by 73 nucleotidesPf6N2E2_1176 and Pf6N2E2_1177 are separated by 12 nucleotidesPf6N2E2_1177 and Pf6N2E2_1178 overlap by 43 nucleotides Pf6N2E2_1175 - VgrG protein, at 1,375,292 to 1,377,517 _1175 Pf6N2E2_1176 - Mll2356 protein, at 1,377,591 to 1,377,986 _1176 Pf6N2E2_1177 - putative exported protein, at 1,377,999 to 1,379,141 _1177 Pf6N2E2_1178 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 1,379,099 to 1,380,202 _1178 Position (kb) 1377 1378 1379 1380Strain fitness (log2 ratio) -3 -2 -1 0 1at 1377.197 kb on - strand, within Pf6N2E2_1175at 1377.395 kb on - strandat 1377.395 kb on - strandat 1377.515 kb on + strandat 1377.515 kb on + strandat 1377.534 kb on - strandat 1377.546 kb on + strandat 1377.683 kb on + strand, within Pf6N2E2_1176at 1377.907 kb on + strand, within Pf6N2E2_1176at 1377.907 kb on + strand, within Pf6N2E2_1176at 1377.908 kb on - strand, within Pf6N2E2_1176at 1377.908 kb on - strand, within Pf6N2E2_1176at 1377.908 kb on - strand, within Pf6N2E2_1176at 1377.908 kb on - strand, within Pf6N2E2_1176at 1377.973 kb on + strandat 1378.037 kb on + strandat 1378.037 kb on + strandat 1378.038 kb on - strandat 1378.096 kb on - strandat 1378.169 kb on - strand, within Pf6N2E2_1177at 1378.204 kb on - strand, within Pf6N2E2_1177at 1378.356 kb on + strand, within Pf6N2E2_1177at 1378.356 kb on + strand, within Pf6N2E2_1177at 1378.602 kb on + strand, within Pf6N2E2_1177at 1378.602 kb on + strand, within Pf6N2E2_1177at 1378.602 kb on + strand, within Pf6N2E2_1177at 1378.602 kb on + strand, within Pf6N2E2_1177at 1378.603 kb on - strand, within Pf6N2E2_1177at 1378.603 kb on - strand, within Pf6N2E2_1177at 1378.603 kb on - strand, within Pf6N2E2_1177at 1378.649 kb on + strand, within Pf6N2E2_1177at 1378.650 kb on - strand, within Pf6N2E2_1177at 1378.668 kb on + strand, within Pf6N2E2_1177at 1378.668 kb on + strand, within Pf6N2E2_1177at 1378.669 kb on - strand, within Pf6N2E2_1177at 1379.458 kb on + strand, within Pf6N2E2_1178at 1379.470 kb on - strand, within Pf6N2E2_1178at 1379.494 kb on - strand, within Pf6N2E2_1178at 1379.494 kb on - strand, within Pf6N2E2_1178at 1379.512 kb on - strand, within Pf6N2E2_1178at 1379.869 kb on + strand, within Pf6N2E2_1178at 1380.072 kb on + strand, within Pf6N2E2_1178at 1380.073 kb on - strand, within Pf6N2E2_1178at 1380.073 kb on - strand, within Pf6N2E2_1178at 1380.102 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 0.5 M
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1,377,197 - Pf6N2E2_1175 0.86 -0.2
1,377,395 - +1.0
1,377,395 - -0.3
1,377,515 + -1.3
1,377,515 + +0.3
1,377,534 - -0.0
1,377,546 + -1.6
1,377,683 + Pf6N2E2_1176 0.23 -3.1
1,377,907 + Pf6N2E2_1176 0.80 +0.4
1,377,907 + Pf6N2E2_1176 0.80 +0.9
1,377,908 - Pf6N2E2_1176 0.80 +0.3
1,377,908 - Pf6N2E2_1176 0.80 -0.4
1,377,908 - Pf6N2E2_1176 0.80 +1.6
1,377,908 - Pf6N2E2_1176 0.80 -0.9
1,377,973 + +0.6
1,378,037 + -0.4
1,378,037 + -1.0
1,378,038 - -1.0
1,378,096 - +0.2
1,378,169 - Pf6N2E2_1177 0.15 -2.4
1,378,204 - Pf6N2E2_1177 0.18 -1.4
1,378,356 + Pf6N2E2_1177 0.31 -1.3
1,378,356 + Pf6N2E2_1177 0.31 -1.0
1,378,602 + Pf6N2E2_1177 0.53 -0.3
1,378,602 + Pf6N2E2_1177 0.53 -1.6
1,378,602 + Pf6N2E2_1177 0.53 -0.0
1,378,602 + Pf6N2E2_1177 0.53 +1.6
1,378,603 - Pf6N2E2_1177 0.53 -1.4
1,378,603 - Pf6N2E2_1177 0.53 -1.2
1,378,603 - Pf6N2E2_1177 0.53 -0.0
1,378,649 + Pf6N2E2_1177 0.57 -0.3
1,378,650 - Pf6N2E2_1177 0.57 -1.0
1,378,668 + Pf6N2E2_1177 0.59 +1.3
1,378,668 + Pf6N2E2_1177 0.59 -0.7
1,378,669 - Pf6N2E2_1177 0.59 -0.0
1,379,458 + Pf6N2E2_1178 0.33 -0.4
1,379,470 - Pf6N2E2_1178 0.34 -0.0
1,379,494 - Pf6N2E2_1178 0.36 -0.8
1,379,494 - Pf6N2E2_1178 0.36 +0.4
1,379,512 - Pf6N2E2_1178 0.37 +0.6
1,379,869 + Pf6N2E2_1178 0.70 -1.4
1,380,072 + Pf6N2E2_1178 0.88 -1.0
1,380,073 - Pf6N2E2_1178 0.88 -0.3
1,380,073 - Pf6N2E2_1178 0.88 +0.4
1,380,102 - -1.0

Or see this region's nucleotide sequence