Experiment: Chloride 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1175 and Pf6N2E2_1176 are separated by 73 nucleotides Pf6N2E2_1176 and Pf6N2E2_1177 are separated by 12 nucleotides Pf6N2E2_1177 and Pf6N2E2_1178 overlap by 43 nucleotides
Pf6N2E2_1175 - VgrG protein, at 1,375,292 to 1,377,517
_1175
Pf6N2E2_1176 - Mll2356 protein, at 1,377,591 to 1,377,986
_1176
Pf6N2E2_1177 - putative exported protein, at 1,377,999 to 1,379,141
_1177
Pf6N2E2_1178 - 3-oxoacyl-(acyl-carrier-protein) synthase, at 1,379,099 to 1,380,202
_1178
Position (kb)
1377
1378
1379
1380 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1377.197 kb on - strand, within Pf6N2E2_1175 at 1377.395 kb on - strand at 1377.395 kb on - strand at 1377.515 kb on + strand at 1377.515 kb on + strand at 1377.534 kb on - strand at 1377.546 kb on + strand at 1377.683 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.973 kb on + strand at 1378.037 kb on + strand at 1378.037 kb on + strand at 1378.038 kb on - strand at 1378.096 kb on - strand at 1378.169 kb on - strand, within Pf6N2E2_1177 at 1378.204 kb on - strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.649 kb on + strand, within Pf6N2E2_1177 at 1378.650 kb on - strand, within Pf6N2E2_1177 at 1378.668 kb on + strand, within Pf6N2E2_1177 at 1378.668 kb on + strand, within Pf6N2E2_1177 at 1378.669 kb on - strand, within Pf6N2E2_1177 at 1379.458 kb on + strand, within Pf6N2E2_1178 at 1379.470 kb on - strand, within Pf6N2E2_1178 at 1379.494 kb on - strand, within Pf6N2E2_1178 at 1379.494 kb on - strand, within Pf6N2E2_1178 at 1379.512 kb on - strand, within Pf6N2E2_1178 at 1379.869 kb on + strand, within Pf6N2E2_1178 at 1380.072 kb on + strand, within Pf6N2E2_1178 at 1380.073 kb on - strand, within Pf6N2E2_1178 at 1380.073 kb on - strand, within Pf6N2E2_1178 at 1380.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 0.5 M remove 1,377,197 - Pf6N2E2_1175 0.86 -0.2 1,377,395 - +1.0 1,377,395 - -0.3 1,377,515 + -1.3 1,377,515 + +0.3 1,377,534 - -0.0 1,377,546 + -1.6 1,377,683 + Pf6N2E2_1176 0.23 -3.1 1,377,907 + Pf6N2E2_1176 0.80 +0.4 1,377,907 + Pf6N2E2_1176 0.80 +0.9 1,377,908 - Pf6N2E2_1176 0.80 +0.3 1,377,908 - Pf6N2E2_1176 0.80 -0.4 1,377,908 - Pf6N2E2_1176 0.80 +1.6 1,377,908 - Pf6N2E2_1176 0.80 -0.9 1,377,973 + +0.6 1,378,037 + -0.4 1,378,037 + -1.0 1,378,038 - -1.0 1,378,096 - +0.2 1,378,169 - Pf6N2E2_1177 0.15 -2.4 1,378,204 - Pf6N2E2_1177 0.18 -1.4 1,378,356 + Pf6N2E2_1177 0.31 -1.3 1,378,356 + Pf6N2E2_1177 0.31 -1.0 1,378,602 + Pf6N2E2_1177 0.53 -0.3 1,378,602 + Pf6N2E2_1177 0.53 -1.6 1,378,602 + Pf6N2E2_1177 0.53 -0.0 1,378,602 + Pf6N2E2_1177 0.53 +1.6 1,378,603 - Pf6N2E2_1177 0.53 -1.4 1,378,603 - Pf6N2E2_1177 0.53 -1.2 1,378,603 - Pf6N2E2_1177 0.53 -0.0 1,378,649 + Pf6N2E2_1177 0.57 -0.3 1,378,650 - Pf6N2E2_1177 0.57 -1.0 1,378,668 + Pf6N2E2_1177 0.59 +1.3 1,378,668 + Pf6N2E2_1177 0.59 -0.7 1,378,669 - Pf6N2E2_1177 0.59 -0.0 1,379,458 + Pf6N2E2_1178 0.33 -0.4 1,379,470 - Pf6N2E2_1178 0.34 -0.0 1,379,494 - Pf6N2E2_1178 0.36 -0.8 1,379,494 - Pf6N2E2_1178 0.36 +0.4 1,379,512 - Pf6N2E2_1178 0.37 +0.6 1,379,869 + Pf6N2E2_1178 0.70 -1.4 1,380,072 + Pf6N2E2_1178 0.88 -1.0 1,380,073 - Pf6N2E2_1178 0.88 -0.3 1,380,073 - Pf6N2E2_1178 0.88 +0.4 1,380,102 - -1.0
Or see this region's nucleotide sequence