Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS18185

Experiment: Temperature shift 30_to_4; with MOPS

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS18180 and PFLU_RS18185 are separated by 144 nucleotidesPFLU_RS18185 and PFLU_RS18190 are separated by 17 nucleotidesPFLU_RS18190 and PFLU_RS18195 are separated by 62 nucleotides PFLU_RS18180: PFLU_RS18180 - sugar ABC transporter ATP-binding protein, at 4,125,764 to 4,127,275 _RS18180 PFLU_RS18185: PFLU_RS18185 - SDR family oxidoreductase, at 4,127,420 to 4,128,184 _RS18185 PFLU_RS18190: PFLU_RS18190 - SDR family oxidoreductase, at 4,128,202 to 4,129,002 _RS18190 PFLU_RS18195: PFLU_RS18195 - sugar-binding transcriptional regulator, at 4,129,065 to 4,130,018 _RS18195 Position (kb) 4127 4128 4129Strain fitness (log2 ratio) -2 -1 0 1 2at 4126.725 kb on + strand, within PFLU_RS18180at 4126.725 kb on + strand, within PFLU_RS18180at 4126.725 kb on + strand, within PFLU_RS18180at 4126.725 kb on + strand, within PFLU_RS18180at 4126.725 kb on + strand, within PFLU_RS18180at 4126.726 kb on - strand, within PFLU_RS18180at 4126.726 kb on - strand, within PFLU_RS18180at 4126.726 kb on - strand, within PFLU_RS18180at 4126.726 kb on - strand, within PFLU_RS18180at 4126.808 kb on - strand, within PFLU_RS18180at 4126.857 kb on - strand, within PFLU_RS18180at 4126.857 kb on - strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.096 kb on + strand, within PFLU_RS18180at 4127.097 kb on - strand, within PFLU_RS18180at 4127.097 kb on - strand, within PFLU_RS18180at 4127.097 kb on - strand, within PFLU_RS18180at 4127.266 kb on + strandat 4127.266 kb on + strandat 4127.266 kb on + strandat 4127.266 kb on + strandat 4127.267 kb on - strandat 4127.274 kb on - strandat 4127.354 kb on - strandat 4127.354 kb on - strandat 4127.538 kb on + strand, within PFLU_RS18185at 4127.539 kb on - strand, within PFLU_RS18185at 4127.539 kb on - strand, within PFLU_RS18185at 4127.897 kb on + strand, within PFLU_RS18185at 4127.897 kb on + strand, within PFLU_RS18185at 4127.897 kb on + strand, within PFLU_RS18185at 4127.897 kb on + strand, within PFLU_RS18185at 4127.897 kb on + strand, within PFLU_RS18185at 4127.897 kb on + strand, within PFLU_RS18185at 4127.898 kb on - strand, within PFLU_RS18185at 4127.898 kb on - strand, within PFLU_RS18185at 4127.898 kb on - strand, within PFLU_RS18185at 4127.994 kb on - strand, within PFLU_RS18185at 4128.222 kb on + strandat 4128.364 kb on + strand, within PFLU_RS18190at 4128.364 kb on + strand, within PFLU_RS18190at 4128.364 kb on + strand, within PFLU_RS18190at 4128.364 kb on + strand, within PFLU_RS18190at 4128.364 kb on + strand, within PFLU_RS18190at 4128.364 kb on + strand, within PFLU_RS18190at 4128.365 kb on - strand, within PFLU_RS18190at 4128.365 kb on - strand, within PFLU_RS18190at 4128.365 kb on - strand, within PFLU_RS18190at 4128.371 kb on + strand, within PFLU_RS18190at 4128.371 kb on + strand, within PFLU_RS18190at 4128.371 kb on + strand, within PFLU_RS18190at 4128.371 kb on + strand, within PFLU_RS18190at 4128.371 kb on + strand, within PFLU_RS18190at 4128.372 kb on - strand, within PFLU_RS18190at 4128.372 kb on - strand, within PFLU_RS18190at 4128.372 kb on - strand, within PFLU_RS18190at 4128.372 kb on - strand, within PFLU_RS18190at 4128.503 kb on - strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.589 kb on + strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.590 kb on - strand, within PFLU_RS18190at 4128.624 kb on + strand, within PFLU_RS18190at 4128.624 kb on + strand, within PFLU_RS18190at 4128.624 kb on + strand, within PFLU_RS18190at 4128.624 kb on + strand, within PFLU_RS18190at 4128.624 kb on + strand, within PFLU_RS18190at 4128.625 kb on - strand, within PFLU_RS18190at 4128.658 kb on + strand, within PFLU_RS18190at 4128.659 kb on - strand, within PFLU_RS18190at 4128.659 kb on - strand, within PFLU_RS18190at 4128.659 kb on - strand, within PFLU_RS18190at 4128.670 kb on + strand, within PFLU_RS18190at 4128.735 kb on + strand, within PFLU_RS18190at 4128.736 kb on - strand, within PFLU_RS18190at 4128.736 kb on - strand, within PFLU_RS18190at 4128.790 kb on + strand, within PFLU_RS18190at 4128.790 kb on + strand, within PFLU_RS18190at 4128.790 kb on + strand, within PFLU_RS18190at 4128.791 kb on - strand, within PFLU_RS18190at 4128.838 kb on + strand, within PFLU_RS18190at 4128.952 kb on + strandat 4128.952 kb on + strandat 4129.001 kb on - strandat 4129.001 kb on - strandat 4129.001 kb on - strandat 4129.040 kb on + strandat 4129.040 kb on + strandat 4129.040 kb on + strandat 4129.040 kb on + strandat 4129.043 kb on + strandat 4129.043 kb on + strandat 4129.044 kb on - strandat 4129.044 kb on - strandat 4129.044 kb on - strandat 4129.045 kb on + strandat 4129.045 kb on + strandat 4129.046 kb on - strandat 4129.046 kb on - strandat 4129.046 kb on - strandat 4129.046 kb on - strandat 4129.046 kb on - strandat 4129.113 kb on + strandat 4129.113 kb on + strandat 4129.113 kb on + strandat 4129.116 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS
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4,126,725 + PFLU_RS18180 0.64 +0.7
4,126,725 + PFLU_RS18180 0.64 +0.7
4,126,725 + PFLU_RS18180 0.64 -0.8
4,126,725 + PFLU_RS18180 0.64 -0.8
4,126,725 + PFLU_RS18180 0.64 +0.6
4,126,726 - PFLU_RS18180 0.64 -0.6
4,126,726 - PFLU_RS18180 0.64 -0.3
4,126,726 - PFLU_RS18180 0.64 -0.3
4,126,726 - PFLU_RS18180 0.64 -0.1
4,126,808 - PFLU_RS18180 0.69 -1.2
4,126,857 - PFLU_RS18180 0.72 -2.6
4,126,857 - PFLU_RS18180 0.72 +0.5
4,127,096 + PFLU_RS18180 0.88 -0.3
4,127,096 + PFLU_RS18180 0.88 +0.0
4,127,096 + PFLU_RS18180 0.88 -0.2
4,127,096 + PFLU_RS18180 0.88 -0.1
4,127,096 + PFLU_RS18180 0.88 +0.2
4,127,096 + PFLU_RS18180 0.88 -0.3
4,127,096 + PFLU_RS18180 0.88 +1.2
4,127,096 + PFLU_RS18180 0.88 +0.3
4,127,096 + PFLU_RS18180 0.88 -0.9
4,127,097 - PFLU_RS18180 0.88 +0.0
4,127,097 - PFLU_RS18180 0.88 +1.1
4,127,097 - PFLU_RS18180 0.88 -1.6
4,127,266 + +0.9
4,127,266 + -0.9
4,127,266 + +0.3
4,127,266 + +0.6
4,127,267 - +0.6
4,127,274 - -0.8
4,127,354 - -0.8
4,127,354 - +0.0
4,127,538 + PFLU_RS18185 0.15 -1.7
4,127,539 - PFLU_RS18185 0.16 +0.1
4,127,539 - PFLU_RS18185 0.16 +0.7
4,127,897 + PFLU_RS18185 0.62 -0.4
4,127,897 + PFLU_RS18185 0.62 -0.0
4,127,897 + PFLU_RS18185 0.62 -0.1
4,127,897 + PFLU_RS18185 0.62 -1.3
4,127,897 + PFLU_RS18185 0.62 -0.0
4,127,897 + PFLU_RS18185 0.62 +0.2
4,127,898 - PFLU_RS18185 0.62 +0.5
4,127,898 - PFLU_RS18185 0.62 +0.1
4,127,898 - PFLU_RS18185 0.62 +0.1
4,127,994 - PFLU_RS18185 0.75 -0.0
4,128,222 + -1.2
4,128,364 + PFLU_RS18190 0.20 +1.2
4,128,364 + PFLU_RS18190 0.20 -1.4
4,128,364 + PFLU_RS18190 0.20 +0.7
4,128,364 + PFLU_RS18190 0.20 -0.3
4,128,364 + PFLU_RS18190 0.20 +0.3
4,128,364 + PFLU_RS18190 0.20 -0.6
4,128,365 - PFLU_RS18190 0.20 +1.2
4,128,365 - PFLU_RS18190 0.20 -2.1
4,128,365 - PFLU_RS18190 0.20 +0.2
4,128,371 + PFLU_RS18190 0.21 +0.9
4,128,371 + PFLU_RS18190 0.21 +0.2
4,128,371 + PFLU_RS18190 0.21 -0.6
4,128,371 + PFLU_RS18190 0.21 +0.7
4,128,371 + PFLU_RS18190 0.21 +0.6
4,128,372 - PFLU_RS18190 0.21 +0.3
4,128,372 - PFLU_RS18190 0.21 -1.3
4,128,372 - PFLU_RS18190 0.21 -0.9
4,128,372 - PFLU_RS18190 0.21 +0.1
4,128,503 - PFLU_RS18190 0.38 -0.2
4,128,589 + PFLU_RS18190 0.48 -0.8
4,128,589 + PFLU_RS18190 0.48 +0.1
4,128,589 + PFLU_RS18190 0.48 -0.6
4,128,589 + PFLU_RS18190 0.48 -0.0
4,128,589 + PFLU_RS18190 0.48 -1.2
4,128,589 + PFLU_RS18190 0.48 -1.1
4,128,589 + PFLU_RS18190 0.48 -1.8
4,128,589 + PFLU_RS18190 0.48 -0.3
4,128,589 + PFLU_RS18190 0.48 -0.1
4,128,589 + PFLU_RS18190 0.48 -1.3
4,128,590 - PFLU_RS18190 0.48 +0.7
4,128,590 - PFLU_RS18190 0.48 -1.3
4,128,590 - PFLU_RS18190 0.48 +1.2
4,128,590 - PFLU_RS18190 0.48 +0.5
4,128,590 - PFLU_RS18190 0.48 -0.8
4,128,590 - PFLU_RS18190 0.48 +0.0
4,128,590 - PFLU_RS18190 0.48 +0.0
4,128,590 - PFLU_RS18190 0.48 +0.1
4,128,590 - PFLU_RS18190 0.48 +0.4
4,128,590 - PFLU_RS18190 0.48 +1.8
4,128,590 - PFLU_RS18190 0.48 -1.6
4,128,590 - PFLU_RS18190 0.48 +0.5
4,128,624 + PFLU_RS18190 0.53 -1.4
4,128,624 + PFLU_RS18190 0.53 -0.5
4,128,624 + PFLU_RS18190 0.53 -0.9
4,128,624 + PFLU_RS18190 0.53 +1.2
4,128,624 + PFLU_RS18190 0.53 +0.4
4,128,625 - PFLU_RS18190 0.53 -0.3
4,128,658 + PFLU_RS18190 0.57 +1.2
4,128,659 - PFLU_RS18190 0.57 +1.0
4,128,659 - PFLU_RS18190 0.57 -0.0
4,128,659 - PFLU_RS18190 0.57 -0.2
4,128,670 + PFLU_RS18190 0.58 -0.4
4,128,735 + PFLU_RS18190 0.67 -0.9
4,128,736 - PFLU_RS18190 0.67 -0.2
4,128,736 - PFLU_RS18190 0.67 +0.2
4,128,790 + PFLU_RS18190 0.73 -0.4
4,128,790 + PFLU_RS18190 0.73 +0.8
4,128,790 + PFLU_RS18190 0.73 +0.7
4,128,791 - PFLU_RS18190 0.74 -1.5
4,128,838 + PFLU_RS18190 0.79 -0.2
4,128,952 + +1.2
4,128,952 + -1.1
4,129,001 - +0.0
4,129,001 - +0.3
4,129,001 - +1.0
4,129,040 + +0.1
4,129,040 + +0.2
4,129,040 + +1.4
4,129,040 + -1.3
4,129,043 + -0.3
4,129,043 + -0.3
4,129,044 - +0.2
4,129,044 - +0.2
4,129,044 - +0.6
4,129,045 + -0.0
4,129,045 + -0.2
4,129,046 - -0.2
4,129,046 - -0.7
4,129,046 - -0.6
4,129,046 - -0.0
4,129,046 - +1.2
4,129,113 + -0.5
4,129,113 + -0.2
4,129,113 + +0.1
4,129,116 + -0.1

Or see this region's nucleotide sequence