Experiment: MoYLS4 with DETA/NO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0337 and DVU0338 are separated by 19 nucleotides DVU0338 and serA are separated by 29 nucleotides serA and DVU0340 are separated by 142 nucleotides DVU0340 and kdsB are separated by 9 nucleotides
DVU0337: DVU0337 - hypothetical protein (TIGR), at 382,107 to 384,077
DVU0337
DVU0338: DVU0338 - hydrolase, haloacid dehalogenase-like family (TIGR), at 384,097 to 384,738
DVU0338
DVU0339: serA - D-isomer specific 2-hydroxyacid dehydrogenase family protein (TIGR), at 384,768 to 385,673
serA
DVU0340: DVU0340 - acetyltransferase, CysE/LacA/LpxA/NodL family (TIGR), at 385,816 to 386,409
DVU0340
DVU0341: kdsB - 3-deoxy-D-manno-octulosonate cytidylyltransferase (TIGR), at 386,419 to 387,189
kdsB
Position (kb) 384
385
386 Strain fitness (log2 ratio) -2
-1
0
1 at 384.026 kb on - strand at 384.026 kb on - strand at 384.121 kb on - strand at 384.771 kb on + strand at 384.779 kb on - strand at 384.833 kb on - strand at 384.865 kb on + strand, within serA at 384.865 kb on + strand, within serA at 384.873 kb on - strand, within serA at 384.899 kb on - strand, within serA at 384.900 kb on - strand, within serA at 384.962 kb on + strand, within serA at 385.034 kb on - strand, within serA at 385.075 kb on - strand, within serA at 385.080 kb on - strand, within serA at 385.101 kb on + strand, within serA at 385.101 kb on + strand, within serA at 385.101 kb on + strand, within serA at 385.123 kb on + strand, within serA at 385.147 kb on + strand, within serA at 385.147 kb on + strand, within serA at 385.177 kb on - strand, within serA at 385.191 kb on - strand, within serA at 385.254 kb on - strand, within serA at 385.269 kb on - strand, within serA at 385.325 kb on + strand, within serA at 385.326 kb on + strand, within serA at 385.333 kb on + strand, within serA at 385.333 kb on + strand, within serA at 385.341 kb on - strand, within serA at 385.431 kb on + strand, within serA at 385.431 kb on + strand, within serA at 385.431 kb on + strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.439 kb on - strand, within serA at 385.530 kb on + strand, within serA at 385.617 kb on + strand at 385.625 kb on - strand at 385.625 kb on - strand at 385.686 kb on + strand at 385.760 kb on + strand at 385.761 kb on + strand at 385.796 kb on - strand at 385.801 kb on - strand at 385.803 kb on + strand at 385.821 kb on + strand at 385.879 kb on - strand, within DVU0340 at 385.879 kb on - strand, within DVU0340 at 385.907 kb on + strand, within DVU0340 at 385.950 kb on - strand, within DVU0340 at 386.159 kb on + strand, within DVU0340 at 386.167 kb on + strand, within DVU0340 at 386.167 kb on - strand, within DVU0340 at 386.191 kb on - strand, within DVU0340 at 386.230 kb on + strand, within DVU0340 at 386.305 kb on - strand, within DVU0340 at 386.333 kb on + strand, within DVU0340 at 386.356 kb on - strand at 386.372 kb on + strand at 386.374 kb on + strand at 386.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DETA/NO 100uM remove 384,026 - +0.0 384,026 - -0.1 384,121 - +0.1 384,771 + -0.2 384,779 - +0.3 384,833 - -1.1 384,865 + serA DVU0339 0.11 -1.0 384,865 + serA DVU0339 0.11 -1.8 384,873 - serA DVU0339 0.12 -0.9 384,899 - serA DVU0339 0.14 -0.9 384,900 - serA DVU0339 0.15 -0.9 384,962 + serA DVU0339 0.21 -1.1 385,034 - serA DVU0339 0.29 -1.0 385,075 - serA DVU0339 0.34 -2.4 385,080 - serA DVU0339 0.34 -0.1 385,101 + serA DVU0339 0.37 -0.6 385,101 + serA DVU0339 0.37 -0.7 385,101 + serA DVU0339 0.37 -1.6 385,123 + serA DVU0339 0.39 -0.7 385,147 + serA DVU0339 0.42 -2.5 385,147 + serA DVU0339 0.42 -0.4 385,177 - serA DVU0339 0.45 -1.1 385,191 - serA DVU0339 0.47 -1.0 385,254 - serA DVU0339 0.54 -1.4 385,269 - serA DVU0339 0.55 -1.3 385,325 + serA DVU0339 0.61 -1.6 385,326 + serA DVU0339 0.62 -1.2 385,333 + serA DVU0339 0.62 -2.0 385,333 + serA DVU0339 0.62 -1.5 385,341 - serA DVU0339 0.63 -1.0 385,431 + serA DVU0339 0.73 -0.4 385,431 + serA DVU0339 0.73 -1.7 385,431 + serA DVU0339 0.73 -1.1 385,439 - serA DVU0339 0.74 -1.4 385,439 - serA DVU0339 0.74 -0.1 385,439 - serA DVU0339 0.74 -0.7 385,439 - serA DVU0339 0.74 -1.3 385,439 - serA DVU0339 0.74 -0.9 385,530 + serA DVU0339 0.84 -0.6 385,617 + -1.1 385,625 - -1.6 385,625 - -1.7 385,686 + +0.3 385,760 + +0.9 385,761 + +0.0 385,796 - +0.1 385,801 - -1.2 385,803 + -0.5 385,821 + -0.7 385,879 - DVU0340 0.11 -0.2 385,879 - DVU0340 0.11 -0.3 385,907 + DVU0340 0.15 -0.5 385,950 - DVU0340 0.23 -0.6 386,159 + DVU0340 0.58 -1.0 386,167 + DVU0340 0.59 -1.4 386,167 - DVU0340 0.59 -1.4 386,191 - DVU0340 0.63 -1.4 386,230 + DVU0340 0.70 -0.4 386,305 - DVU0340 0.82 -2.4 386,333 + DVU0340 0.87 -1.4 386,356 - -0.4 386,372 + -1.9 386,374 + -0.6 386,382 - -1.1
Or see this region's nucleotide sequence