Experiment: MoYLS4 with DETA/NO 100uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1442 and flgE are separated by 467 nucleotides flgE and flgD are separated by 57 nucleotides flgD and DVU1445 are separated by 12 nucleotides
DVU1442: DVU1442 - flagellin FlaG, putative (TIGR), at 1,518,983 to 1,519,357
DVU1442
DVU1443: flgE - flagellar hook protein FlgE (TIGR), at 1,519,825 to 1,521,519
flgE
DVU1444: flgD - basal-body rod modification protein FlgD (TIGR), at 1,521,577 to 1,522,374
flgD
DVU1445: DVU1445 - flagellar hook-length control domain protein (TIGR), at 1,522,387 to 1,523,982
DVU1445
Position (kb)
1519
1520
1521
1522 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1518.830 kb on - strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.907 kb on + strand at 1518.915 kb on - strand at 1519.002 kb on + strand at 1519.196 kb on + strand, within DVU1442 at 1519.214 kb on + strand, within DVU1442 at 1519.214 kb on + strand, within DVU1442 at 1519.240 kb on - strand, within DVU1442 at 1519.268 kb on - strand, within DVU1442 at 1519.303 kb on + strand, within DVU1442 at 1519.337 kb on + strand at 1519.345 kb on - strand at 1519.345 kb on - strand at 1519.345 kb on - strand at 1519.345 kb on - strand at 1519.467 kb on - strand at 1519.618 kb on - strand at 1519.699 kb on + strand at 1519.701 kb on + strand at 1519.738 kb on - strand at 1519.738 kb on - strand at 1519.831 kb on - strand at 1519.912 kb on + strand at 1519.961 kb on - strand at 1520.083 kb on - strand, within flgE at 1520.139 kb on + strand, within flgE at 1520.248 kb on + strand, within flgE at 1520.323 kb on + strand, within flgE at 1520.380 kb on + strand, within flgE at 1520.380 kb on + strand, within flgE at 1520.380 kb on + strand, within flgE at 1520.527 kb on - strand, within flgE at 1520.614 kb on - strand, within flgE at 1520.659 kb on + strand, within flgE at 1520.700 kb on + strand, within flgE at 1520.786 kb on + strand, within flgE at 1520.794 kb on - strand, within flgE at 1520.826 kb on - strand, within flgE at 1520.864 kb on + strand, within flgE at 1520.938 kb on - strand, within flgE at 1520.994 kb on - strand, within flgE at 1521.135 kb on - strand, within flgE at 1521.176 kb on + strand, within flgE at 1521.236 kb on + strand, within flgE at 1521.298 kb on + strand, within flgE at 1521.306 kb on - strand, within flgE at 1521.378 kb on - strand at 1521.422 kb on + strand at 1521.430 kb on - strand at 1521.488 kb on - strand at 1521.488 kb on - strand at 1521.488 kb on - strand at 1521.514 kb on + strand at 1521.649 kb on - strand at 1521.680 kb on - strand, within flgD at 1521.711 kb on + strand, within flgD at 1521.721 kb on - strand, within flgD at 1521.788 kb on - strand, within flgD at 1521.807 kb on + strand, within flgD at 1521.827 kb on + strand, within flgD at 1521.835 kb on - strand, within flgD at 1521.878 kb on - strand, within flgD at 1521.884 kb on + strand, within flgD at 1521.927 kb on - strand, within flgD at 1522.083 kb on - strand, within flgD at 1522.114 kb on - strand, within flgD at 1522.160 kb on - strand, within flgD at 1522.184 kb on - strand, within flgD at 1522.184 kb on - strand, within flgD at 1522.238 kb on - strand, within flgD at 1522.320 kb on - strand at 1522.398 kb on + strand at 1522.469 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DETA/NO 100uM remove 1,518,830 - +0.1 1,518,907 + -0.6 1,518,907 + +0.8 1,518,907 + +0.1 1,518,907 + +0.1 1,518,907 + -0.9 1,518,907 + -0.0 1,518,907 + +1.2 1,518,907 + +0.1 1,518,915 - +0.2 1,519,002 + +0.5 1,519,196 + DVU1442 0.57 +0.1 1,519,214 + DVU1442 0.62 -1.1 1,519,214 + DVU1442 0.62 -0.0 1,519,240 - DVU1442 0.69 -0.2 1,519,268 - DVU1442 0.76 +0.3 1,519,303 + DVU1442 0.85 -0.5 1,519,337 + +1.0 1,519,345 - +0.0 1,519,345 - -0.3 1,519,345 - -0.2 1,519,345 - -0.5 1,519,467 - +0.8 1,519,618 - -0.5 1,519,699 + -0.2 1,519,701 + -0.4 1,519,738 - -1.0 1,519,738 - +0.1 1,519,831 - +1.6 1,519,912 + -0.6 1,519,961 - -0.9 1,520,083 - flgE DVU1443 0.15 +0.6 1,520,139 + flgE DVU1443 0.19 -1.0 1,520,248 + flgE DVU1443 0.25 -0.2 1,520,323 + flgE DVU1443 0.29 -0.7 1,520,380 + flgE DVU1443 0.33 -1.0 1,520,380 + flgE DVU1443 0.33 +0.6 1,520,380 + flgE DVU1443 0.33 -0.4 1,520,527 - flgE DVU1443 0.41 -0.8 1,520,614 - flgE DVU1443 0.47 -0.5 1,520,659 + flgE DVU1443 0.49 -0.4 1,520,700 + flgE DVU1443 0.52 -0.4 1,520,786 + flgE DVU1443 0.57 -1.0 1,520,794 - flgE DVU1443 0.57 +0.6 1,520,826 - flgE DVU1443 0.59 -1.0 1,520,864 + flgE DVU1443 0.61 -3.9 1,520,938 - flgE DVU1443 0.66 -1.0 1,520,994 - flgE DVU1443 0.69 -1.1 1,521,135 - flgE DVU1443 0.77 -0.5 1,521,176 + flgE DVU1443 0.80 -0.8 1,521,236 + flgE DVU1443 0.83 +0.6 1,521,298 + flgE DVU1443 0.87 +0.6 1,521,306 - flgE DVU1443 0.87 -1.6 1,521,378 - -0.8 1,521,422 + -0.4 1,521,430 - -0.6 1,521,488 - -0.9 1,521,488 - -0.7 1,521,488 - -1.2 1,521,514 + -0.4 1,521,649 - -0.5 1,521,680 - flgD DVU1444 0.13 +0.1 1,521,711 + flgD DVU1444 0.17 -0.2 1,521,721 - flgD DVU1444 0.18 +0.1 1,521,788 - flgD DVU1444 0.26 -0.6 1,521,807 + flgD DVU1444 0.29 -1.0 1,521,827 + flgD DVU1444 0.31 -1.0 1,521,835 - flgD DVU1444 0.32 -0.1 1,521,878 - flgD DVU1444 0.38 -2.0 1,521,884 + flgD DVU1444 0.38 -1.0 1,521,927 - flgD DVU1444 0.44 -1.3 1,522,083 - flgD DVU1444 0.63 -0.3 1,522,114 - flgD DVU1444 0.67 -1.1 1,522,160 - flgD DVU1444 0.73 -1.0 1,522,184 - flgD DVU1444 0.76 -1.3 1,522,184 - flgD DVU1444 0.76 -0.9 1,522,238 - flgD DVU1444 0.83 -1.3 1,522,320 - -0.6 1,522,398 + -0.9 1,522,469 + -0.9
Or see this region's nucleotide sequence