Experiment: Low light with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2322 and Rru_A2323 are separated by 138 nucleotides Rru_A2323 and Rru_A2324 are separated by 52 nucleotides Rru_A2324 and Rru_A2325 are separated by 35 nucleotides
Rru_A2322: Rru_A2322 - CheW protein (NCBI), at 2,691,896 to 2,693,497
_A2322
Rru_A2323: Rru_A2323 - Two component CheB methylesterase (NCBI), at 2,693,636 to 2,694,748
_A2323
Rru_A2324: Rru_A2324 - PBS lyase HEAT-like repeat (NCBI), at 2,694,801 to 2,695,349
_A2324
Rru_A2325: Rru_A2325 - MCP methyltransferase, CheR-type (NCBI), at 2,695,385 to 2,696,215
_A2325
Position (kb)
2693
2694
2695 Strain fitness (log2 ratio)
-2
-1
0
1 at 2693.021 kb on + strand, within Rru_A2322 at 2693.081 kb on + strand, within Rru_A2322 at 2693.081 kb on + strand, within Rru_A2322 at 2693.270 kb on + strand, within Rru_A2322 at 2693.270 kb on + strand, within Rru_A2322 at 2693.495 kb on + strand at 2693.495 kb on + strand at 2693.526 kb on + strand at 2693.559 kb on + strand at 2693.622 kb on - strand at 2694.028 kb on + strand, within Rru_A2323 at 2694.029 kb on - strand, within Rru_A2323 at 2694.297 kb on + strand, within Rru_A2323 at 2694.297 kb on + strand, within Rru_A2323 at 2694.297 kb on + strand, within Rru_A2323 at 2694.297 kb on + strand, within Rru_A2323 at 2694.297 kb on + strand, within Rru_A2323 at 2694.297 kb on + strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.298 kb on - strand, within Rru_A2323 at 2694.357 kb on - strand, within Rru_A2323 at 2694.440 kb on + strand, within Rru_A2323 at 2694.440 kb on + strand, within Rru_A2323 at 2694.441 kb on - strand, within Rru_A2323 at 2694.441 kb on - strand, within Rru_A2323 at 2694.441 kb on - strand, within Rru_A2323 at 2694.728 kb on + strand at 2694.728 kb on + strand at 2694.728 kb on + strand at 2694.728 kb on + strand at 2694.729 kb on - strand at 2694.729 kb on - strand at 2695.196 kb on + strand, within Rru_A2324 at 2695.197 kb on - strand, within Rru_A2324 at 2695.197 kb on - strand, within Rru_A2324 at 2695.197 kb on - strand, within Rru_A2324 at 2695.431 kb on - strand at 2695.556 kb on - strand, within Rru_A2325 at 2695.610 kb on + strand, within Rru_A2325 at 2695.611 kb on - strand, within Rru_A2325 at 2695.628 kb on + strand, within Rru_A2325 at 2695.629 kb on - strand, within Rru_A2325
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with N2 remove 2,693,021 + Rru_A2322 0.70 -0.4 2,693,081 + Rru_A2322 0.74 +0.1 2,693,081 + Rru_A2322 0.74 -1.1 2,693,270 + Rru_A2322 0.86 +0.1 2,693,270 + Rru_A2322 0.86 +0.8 2,693,495 + +0.0 2,693,495 + +0.3 2,693,526 + +0.2 2,693,559 + -0.8 2,693,622 - +0.0 2,694,028 + Rru_A2323 0.35 +0.1 2,694,029 - Rru_A2323 0.35 +0.1 2,694,297 + Rru_A2323 0.59 +0.2 2,694,297 + Rru_A2323 0.59 +0.2 2,694,297 + Rru_A2323 0.59 -0.1 2,694,297 + Rru_A2323 0.59 -1.4 2,694,297 + Rru_A2323 0.59 -0.2 2,694,297 + Rru_A2323 0.59 -0.1 2,694,298 - Rru_A2323 0.59 -0.1 2,694,298 - Rru_A2323 0.59 -0.3 2,694,298 - Rru_A2323 0.59 -0.3 2,694,298 - Rru_A2323 0.59 -0.2 2,694,298 - Rru_A2323 0.59 -0.1 2,694,298 - Rru_A2323 0.59 -0.3 2,694,298 - Rru_A2323 0.59 +0.3 2,694,298 - Rru_A2323 0.59 -0.0 2,694,357 - Rru_A2323 0.65 +0.4 2,694,440 + Rru_A2323 0.72 +0.4 2,694,440 + Rru_A2323 0.72 -1.0 2,694,441 - Rru_A2323 0.72 +0.1 2,694,441 - Rru_A2323 0.72 +0.8 2,694,441 - Rru_A2323 0.72 -0.2 2,694,728 + -2.5 2,694,728 + -0.4 2,694,728 + -0.2 2,694,728 + -0.1 2,694,729 - +0.0 2,694,729 - +1.2 2,695,196 + Rru_A2324 0.72 +0.2 2,695,197 - Rru_A2324 0.72 -0.2 2,695,197 - Rru_A2324 0.72 -0.8 2,695,197 - Rru_A2324 0.72 +0.0 2,695,431 - -0.1 2,695,556 - Rru_A2325 0.21 +0.1 2,695,610 + Rru_A2325 0.27 -0.2 2,695,611 - Rru_A2325 0.27 -0.9 2,695,628 + Rru_A2325 0.29 -0.1 2,695,629 - Rru_A2325 0.29 +0.8
Or see this region's nucleotide sequence