Experiment: Low light with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1614 and Rru_A1615 are separated by 27 nucleotides Rru_A1615 and Rru_A1616 are separated by 61 nucleotides Rru_A1616 and Rru_A1617 are separated by 335 nucleotides
Rru_A1614: Rru_A1614 - hypothetical protein (NCBI), at 1,908,169 to 1,908,468
_A1614
Rru_A1615: Rru_A1615 - 3-mercaptopyruvate sulfurtransferase (NCBI), at 1,908,496 to 1,909,353
_A1615
Rru_A1616: Rru_A1616 - Pyridoxal-5'-phosphate-dependent enzyme, beta subunit (NCBI), at 1,909,415 to 1,910,398
_A1616
Rru_A1617: Rru_A1617 - Protein of unknown function DUF1289 (NCBI), at 1,910,734 to 1,911,645
_A1617
Position (kb)
1909
1910
1911 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1908.467 kb on - strand at 1908.467 kb on - strand at 1908.521 kb on - strand at 1908.551 kb on + strand at 1908.551 kb on + strand at 1908.552 kb on - strand at 1908.734 kb on + strand, within Rru_A1615 at 1908.734 kb on + strand, within Rru_A1615 at 1908.734 kb on + strand, within Rru_A1615 at 1908.760 kb on - strand, within Rru_A1615 at 1908.760 kb on - strand, within Rru_A1615 at 1908.891 kb on + strand, within Rru_A1615 at 1908.891 kb on + strand, within Rru_A1615 at 1908.891 kb on + strand, within Rru_A1615 at 1909.071 kb on - strand, within Rru_A1615 at 1909.210 kb on - strand, within Rru_A1615 at 1909.224 kb on - strand, within Rru_A1615 at 1909.262 kb on + strand, within Rru_A1615 at 1909.262 kb on + strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.541 kb on - strand, within Rru_A1616 at 1909.589 kb on + strand, within Rru_A1616 at 1909.590 kb on - strand, within Rru_A1616 at 1909.765 kb on + strand, within Rru_A1616 at 1909.765 kb on + strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.767 kb on + strand, within Rru_A1616 at 1909.768 kb on - strand, within Rru_A1616 at 1909.768 kb on - strand, within Rru_A1616 at 1909.954 kb on + strand, within Rru_A1616 at 1910.019 kb on + strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.035 kb on + strand, within Rru_A1616 at 1910.036 kb on - strand, within Rru_A1616 at 1910.036 kb on - strand, within Rru_A1616 at 1910.038 kb on - strand, within Rru_A1616 at 1910.038 kb on - strand, within Rru_A1616 at 1910.038 kb on - strand, within Rru_A1616 at 1910.350 kb on + strand at 1910.350 kb on + strand at 1910.351 kb on - strand at 1910.571 kb on + strand at 1910.572 kb on - strand at 1910.572 kb on - strand at 1910.572 kb on - strand at 1910.572 kb on - strand at 1910.583 kb on + strand at 1910.584 kb on - strand at 1910.612 kb on + strand at 1910.613 kb on - strand at 1910.613 kb on - strand at 1910.704 kb on + strand at 1910.761 kb on - strand at 1911.193 kb on + strand, within Rru_A1617 at 1911.193 kb on + strand, within Rru_A1617 at 1911.193 kb on + strand, within Rru_A1617 at 1911.194 kb on - strand, within Rru_A1617
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with N2 remove 1,908,467 - -0.2 1,908,467 - +0.8 1,908,521 - +0.8 1,908,551 + +0.6 1,908,551 + +0.2 1,908,552 - +2.3 1,908,734 + Rru_A1615 0.28 -0.2 1,908,734 + Rru_A1615 0.28 -0.3 1,908,734 + Rru_A1615 0.28 -0.2 1,908,760 - Rru_A1615 0.31 +0.8 1,908,760 - Rru_A1615 0.31 +0.8 1,908,891 + Rru_A1615 0.46 -1.3 1,908,891 + Rru_A1615 0.46 -0.1 1,908,891 + Rru_A1615 0.46 -0.5 1,909,071 - Rru_A1615 0.67 -0.0 1,909,210 - Rru_A1615 0.83 -0.1 1,909,224 - Rru_A1615 0.85 +0.6 1,909,262 + Rru_A1615 0.89 -0.3 1,909,262 + Rru_A1615 0.89 -0.2 1,909,263 - Rru_A1615 0.89 -0.3 1,909,263 - Rru_A1615 0.89 +0.8 1,909,263 - Rru_A1615 0.89 -0.0 1,909,263 - Rru_A1615 0.89 -0.0 1,909,263 - Rru_A1615 0.89 +0.5 1,909,263 - Rru_A1615 0.89 -0.3 1,909,263 - Rru_A1615 0.89 +0.9 1,909,377 + -0.5 1,909,377 + -0.2 1,909,377 + -0.4 1,909,377 + +1.5 1,909,377 + +0.4 1,909,377 + -0.1 1,909,378 - +0.0 1,909,378 - +0.3 1,909,378 - -0.8 1,909,378 - -0.2 1,909,378 - -0.7 1,909,378 - +0.8 1,909,541 - Rru_A1616 0.13 -0.4 1,909,589 + Rru_A1616 0.18 -0.6 1,909,590 - Rru_A1616 0.18 +0.1 1,909,765 + Rru_A1616 0.36 -0.1 1,909,765 + Rru_A1616 0.36 +0.8 1,909,766 - Rru_A1616 0.36 -0.3 1,909,766 - Rru_A1616 0.36 -0.0 1,909,766 - Rru_A1616 0.36 -0.3 1,909,766 - Rru_A1616 0.36 +0.1 1,909,766 - Rru_A1616 0.36 +0.1 1,909,766 - Rru_A1616 0.36 +0.6 1,909,767 + Rru_A1616 0.36 -0.4 1,909,768 - Rru_A1616 0.36 -0.4 1,909,768 - Rru_A1616 0.36 -0.1 1,909,954 + Rru_A1616 0.55 +0.8 1,910,019 + Rru_A1616 0.61 -0.2 1,910,020 - Rru_A1616 0.61 -0.0 1,910,020 - Rru_A1616 0.61 -0.2 1,910,020 - Rru_A1616 0.61 -1.1 1,910,020 - Rru_A1616 0.61 -0.2 1,910,020 - Rru_A1616 0.61 -0.4 1,910,035 + Rru_A1616 0.63 +0.2 1,910,036 - Rru_A1616 0.63 +1.3 1,910,036 - Rru_A1616 0.63 +0.2 1,910,038 - Rru_A1616 0.63 -0.3 1,910,038 - Rru_A1616 0.63 -0.6 1,910,038 - Rru_A1616 0.63 -0.0 1,910,350 + +0.8 1,910,350 + +0.8 1,910,351 - -0.1 1,910,571 + -0.4 1,910,572 - -1.2 1,910,572 - +0.1 1,910,572 - -0.8 1,910,572 - -1.9 1,910,583 + +0.1 1,910,584 - -0.1 1,910,612 + -0.4 1,910,613 - +1.9 1,910,613 - +0.8 1,910,704 + -0.2 1,910,761 - -0.3 1,911,193 + Rru_A1617 0.50 +0.8 1,911,193 + Rru_A1617 0.50 -1.1 1,911,193 + Rru_A1617 0.50 -2.0 1,911,194 - Rru_A1617 0.50 +0.2
Or see this region's nucleotide sequence