Experiment: Low light with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1469 and Rru_A1470 are separated by 3 nucleotides Rru_A1470 and Rru_A1471 are separated by 73 nucleotides Rru_A1471 and Rru_A1472 overlap by 8 nucleotides Rru_A1472 and Rru_A1473 are separated by 104 nucleotides
Rru_A1469: Rru_A1469 - Acetyl-CoA C-acetyltransferase (NCBI), at 1,736,050 to 1,737,225
_A1469
Rru_A1470: Rru_A1470 - hypothetical protein (NCBI), at 1,737,229 to 1,737,510
_A1470
Rru_A1471: Rru_A1471 - Butyrate-acetoacetate CoA-transferase (NCBI), at 1,737,584 to 1,738,243
_A1471
Rru_A1472: Rru_A1472 - Acetate CoA-transferase (NCBI), at 1,738,236 to 1,738,898
_A1472
Rru_A1473: Rru_A1473 - hypothetical protein (NCBI), at 1,739,003 to 1,739,317
_A1473
Position (kb)
1737
1738
1739 Strain fitness (log2 ratio)
-1
0
1 at 1737.144 kb on + strand at 1737.435 kb on + strand, within Rru_A1470 at 1737.436 kb on - strand, within Rru_A1470 at 1737.559 kb on + strand at 1737.812 kb on - strand, within Rru_A1471 at 1738.143 kb on + strand, within Rru_A1471 at 1738.143 kb on + strand, within Rru_A1471 at 1738.143 kb on + strand, within Rru_A1471 at 1738.144 kb on - strand, within Rru_A1471 at 1738.144 kb on - strand, within Rru_A1471 at 1738.797 kb on - strand, within Rru_A1472 at 1738.797 kb on - strand, within Rru_A1472 at 1739.004 kb on + strand at 1739.005 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with N2 remove 1,737,144 + -0.7 1,737,435 + Rru_A1470 0.73 -0.4 1,737,436 - Rru_A1470 0.73 +0.5 1,737,559 + +0.3 1,737,812 - Rru_A1471 0.35 +0.8 1,738,143 + Rru_A1471 0.85 -0.6 1,738,143 + Rru_A1471 0.85 -0.6 1,738,143 + Rru_A1471 0.85 -0.0 1,738,144 - Rru_A1471 0.85 -0.4 1,738,144 - Rru_A1471 0.85 -0.6 1,738,797 - Rru_A1472 0.85 +0.8 1,738,797 - Rru_A1472 0.85 +0.8 1,739,004 + +0.6 1,739,005 - -0.3
Or see this region's nucleotide sequence