Experiment: Low light with N2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0074 and Rru_A0075 overlap by 4 nucleotides Rru_A0075 and Rru_A0076 are separated by 155 nucleotides Rru_A0076 and Rru_A0077 are separated by 177 nucleotides
Rru_A0074: Rru_A0074 - Helicase-like (NCBI), at 85,794 to 88,994
_A0074
Rru_A0075: Rru_A0075 - RNA-binding S4 (NCBI), at 88,991 to 89,425
_A0075
Rru_A0076: Rru_A0076 - Protein of unknown function DUF1332 (NCBI), at 89,581 to 90,036
_A0076
Rru_A0077: Rru_A0077 - 4Fe-4S ferredoxin, iron-sulfur binding (NCBI), at 90,214 to 90,552
_A0077
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-2
-1
0
1 at 88.029 kb on - strand, within Rru_A0074 at 88.029 kb on - strand, within Rru_A0074 at 88.029 kb on - strand, within Rru_A0074 at 88.029 kb on - strand, within Rru_A0074 at 88.029 kb on - strand, within Rru_A0074 at 88.576 kb on - strand, within Rru_A0074 at 88.867 kb on - strand at 88.911 kb on + strand at 88.911 kb on + strand at 88.912 kb on - strand at 88.955 kb on + strand at 88.956 kb on - strand at 88.972 kb on + strand at 88.972 kb on + strand at 88.973 kb on - strand at 89.074 kb on + strand, within Rru_A0075 at 89.074 kb on + strand, within Rru_A0075 at 89.075 kb on - strand, within Rru_A0075 at 89.087 kb on + strand, within Rru_A0075 at 89.088 kb on - strand, within Rru_A0075 at 89.294 kb on + strand, within Rru_A0075 at 89.295 kb on - strand, within Rru_A0075 at 89.423 kb on + strand at 89.467 kb on + strand at 89.467 kb on + strand at 89.468 kb on - strand at 89.468 kb on - strand at 89.580 kb on + strand at 89.580 kb on + strand at 89.580 kb on + strand at 89.580 kb on + strand at 89.580 kb on + strand at 89.580 kb on + strand at 89.581 kb on - strand at 89.828 kb on - strand, within Rru_A0076 at 89.863 kb on + strand, within Rru_A0076 at 89.908 kb on + strand, within Rru_A0076 at 89.908 kb on + strand, within Rru_A0076 at 89.909 kb on - strand, within Rru_A0076 at 89.909 kb on - strand, within Rru_A0076 at 89.909 kb on - strand, within Rru_A0076 at 89.971 kb on + strand, within Rru_A0076 at 89.972 kb on - strand, within Rru_A0076 at 89.972 kb on - strand, within Rru_A0076 at 89.972 kb on - strand, within Rru_A0076 at 89.972 kb on - strand, within Rru_A0076 at 89.972 kb on - strand, within Rru_A0076 at 90.004 kb on - strand at 90.037 kb on + strand at 90.037 kb on + strand at 90.037 kb on + strand at 90.037 kb on + strand at 90.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Low light with N2 remove 88,029 - Rru_A0074 0.70 +0.9 88,029 - Rru_A0074 0.70 +0.9 88,029 - Rru_A0074 0.70 -0.2 88,029 - Rru_A0074 0.70 +0.4 88,029 - Rru_A0074 0.70 -0.9 88,576 - Rru_A0074 0.87 +0.6 88,867 - +0.2 88,911 + -0.2 88,911 + +0.0 88,912 - +0.9 88,955 + -0.1 88,956 - +0.9 88,972 + +0.9 88,972 + -0.6 88,973 - -0.3 89,074 + Rru_A0075 0.19 -0.7 89,074 + Rru_A0075 0.19 -1.1 89,075 - Rru_A0075 0.19 -0.2 89,087 + Rru_A0075 0.22 -1.0 89,088 - Rru_A0075 0.22 -1.3 89,294 + Rru_A0075 0.70 +0.1 89,295 - Rru_A0075 0.70 +0.9 89,423 + -0.2 89,467 + -0.1 89,467 + -0.2 89,468 - +0.9 89,468 - -0.2 89,580 + -0.7 89,580 + +0.2 89,580 + -0.4 89,580 + +0.2 89,580 + +0.2 89,580 + -2.3 89,581 - -0.3 89,828 - Rru_A0076 0.54 -0.2 89,863 + Rru_A0076 0.62 -0.1 89,908 + Rru_A0076 0.72 +1.3 89,908 + Rru_A0076 0.72 +0.9 89,909 - Rru_A0076 0.72 +0.5 89,909 - Rru_A0076 0.72 -0.4 89,909 - Rru_A0076 0.72 +0.4 89,971 + Rru_A0076 0.86 -0.2 89,972 - Rru_A0076 0.86 +0.9 89,972 - Rru_A0076 0.86 -0.1 89,972 - Rru_A0076 0.86 +0.2 89,972 - Rru_A0076 0.86 -0.4 89,972 - Rru_A0076 0.86 +0.2 90,004 - +0.9 90,037 + -0.0 90,037 + +0.9 90,037 + -0.1 90,037 + +0.3 90,037 + -0.1
Or see this region's nucleotide sequence