Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_864 and Pf6N2E2_865 are separated by 3 nucleotides Pf6N2E2_865 and Pf6N2E2_866 are separated by 22 nucleotides Pf6N2E2_866 and Pf6N2E2_867 are separated by 275 nucleotides
Pf6N2E2_864 - 2-keto-4-pentenoate hydratase (EC 4.2.1.80), at 1,012,725 to 1,013,525
_864
Pf6N2E2_865 - Fumarylacetoacetate hydrolase family protein, at 1,013,529 to 1,014,422
_865
Pf6N2E2_866 - hypothetical protein, at 1,014,445 to 1,015,140
_866
Pf6N2E2_867 - PhaK-like protein, at 1,015,416 to 1,016,696
_867
Position (kb)
1014
1015
1016 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1013.537 kb on - strand at 1013.537 kb on - strand at 1013.767 kb on - strand, within Pf6N2E2_865 at 1014.195 kb on - strand, within Pf6N2E2_865 at 1014.204 kb on + strand, within Pf6N2E2_865 at 1014.366 kb on + strand at 1014.366 kb on + strand at 1014.367 kb on - strand at 1014.367 kb on - strand at 1014.444 kb on + strand at 1014.468 kb on - strand at 1014.468 kb on - strand at 1014.514 kb on + strand at 1014.514 kb on + strand at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.515 kb on - strand, within Pf6N2E2_866 at 1014.611 kb on - strand, within Pf6N2E2_866 at 1014.611 kb on - strand, within Pf6N2E2_866 at 1014.622 kb on + strand, within Pf6N2E2_866 at 1014.622 kb on + strand, within Pf6N2E2_866 at 1014.622 kb on + strand, within Pf6N2E2_866 at 1014.661 kb on + strand, within Pf6N2E2_866 at 1014.661 kb on + strand, within Pf6N2E2_866 at 1014.661 kb on + strand, within Pf6N2E2_866 at 1014.751 kb on + strand, within Pf6N2E2_866 at 1014.751 kb on + strand, within Pf6N2E2_866 at 1014.778 kb on + strand, within Pf6N2E2_866 at 1014.819 kb on + strand, within Pf6N2E2_866 at 1014.819 kb on + strand, within Pf6N2E2_866 at 1014.819 kb on + strand, within Pf6N2E2_866 at 1014.820 kb on - strand, within Pf6N2E2_866 at 1014.820 kb on - strand, within Pf6N2E2_866 at 1014.879 kb on + strand, within Pf6N2E2_866 at 1014.882 kb on + strand, within Pf6N2E2_866 at 1014.882 kb on + strand, within Pf6N2E2_866 at 1014.883 kb on - strand, within Pf6N2E2_866 at 1015.009 kb on + strand, within Pf6N2E2_866 at 1015.010 kb on - strand, within Pf6N2E2_866 at 1015.093 kb on + strand at 1015.116 kb on + strand at 1015.210 kb on + strand at 1015.210 kb on + strand at 1015.210 kb on + strand at 1015.269 kb on - strand at 1015.277 kb on - strand at 1015.394 kb on + strand at 1015.572 kb on + strand, within Pf6N2E2_867 at 1015.572 kb on + strand, within Pf6N2E2_867 at 1015.708 kb on - strand, within Pf6N2E2_867 at 1015.708 kb on - strand, within Pf6N2E2_867
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 1,013,537 - +1.3 1,013,537 - +1.5 1,013,767 - Pf6N2E2_865 0.27 -0.7 1,014,195 - Pf6N2E2_865 0.74 -0.3 1,014,204 + Pf6N2E2_865 0.76 +1.9 1,014,366 + +0.2 1,014,366 + -0.7 1,014,367 - -1.9 1,014,367 - -0.8 1,014,444 + -0.6 1,014,468 - -0.3 1,014,468 - -1.1 1,014,514 + -1.1 1,014,514 + +0.8 1,014,515 - Pf6N2E2_866 0.10 -0.8 1,014,515 - Pf6N2E2_866 0.10 -0.1 1,014,515 - Pf6N2E2_866 0.10 -1.1 1,014,515 - Pf6N2E2_866 0.10 -1.5 1,014,611 - Pf6N2E2_866 0.24 +0.2 1,014,611 - Pf6N2E2_866 0.24 +2.3 1,014,622 + Pf6N2E2_866 0.25 -0.8 1,014,622 + Pf6N2E2_866 0.25 +0.5 1,014,622 + Pf6N2E2_866 0.25 +0.1 1,014,661 + Pf6N2E2_866 0.31 +0.1 1,014,661 + Pf6N2E2_866 0.31 -2.0 1,014,661 + Pf6N2E2_866 0.31 +0.1 1,014,751 + Pf6N2E2_866 0.44 +1.1 1,014,751 + Pf6N2E2_866 0.44 -2.2 1,014,778 + Pf6N2E2_866 0.48 -1.2 1,014,819 + Pf6N2E2_866 0.54 +0.3 1,014,819 + Pf6N2E2_866 0.54 -0.5 1,014,819 + Pf6N2E2_866 0.54 -0.3 1,014,820 - Pf6N2E2_866 0.54 -0.2 1,014,820 - Pf6N2E2_866 0.54 -0.8 1,014,879 + Pf6N2E2_866 0.62 +0.1 1,014,882 + Pf6N2E2_866 0.63 -0.3 1,014,882 + Pf6N2E2_866 0.63 +1.1 1,014,883 - Pf6N2E2_866 0.63 -0.8 1,015,009 + Pf6N2E2_866 0.81 -1.7 1,015,010 - Pf6N2E2_866 0.81 -1.7 1,015,093 + +0.5 1,015,116 + +2.5 1,015,210 + -0.6 1,015,210 + +0.1 1,015,210 + -0.3 1,015,269 - -0.1 1,015,277 - -1.1 1,015,394 + +0.7 1,015,572 + Pf6N2E2_867 0.12 -1.1 1,015,572 + Pf6N2E2_867 0.12 +0.5 1,015,708 - Pf6N2E2_867 0.23 -0.1 1,015,708 - Pf6N2E2_867 0.23 +0.6
Or see this region's nucleotide sequence