Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_815

Experiment: dimethyl sulfone 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_812 and Pf6N2E2_813 are separated by 77 nucleotidesPf6N2E2_813 and Pf6N2E2_814 are separated by 212 nucleotidesPf6N2E2_814 and Pf6N2E2_815 overlap by 30 nucleotidesPf6N2E2_815 and Pf6N2E2_816 overlap by 1 nucleotidesPf6N2E2_816 and Pf6N2E2_817 overlap by 4 nucleotides Pf6N2E2_812 - Glycosyltransferase, at 944,886 to 946,178 _812 Pf6N2E2_813 - Response regulator, at 946,256 to 946,561 _813 Pf6N2E2_814 - hypothetical protein, at 946,774 to 946,917 _814 Pf6N2E2_815 - Signal transduction histidine kinase, at 946,888 to 950,370 _815 Pf6N2E2_816 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 950,370 to 951,185 _816 Pf6N2E2_817 - Protein-glutamate methylesterase (EC 3.1.1.61), at 951,182 to 951,769 _817 Position (kb) 946 947 948 949 950 951Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 946.138 kb on + strandat 946.138 kb on + strandat 946.138 kb on + strandat 946.138 kb on + strandat 946.139 kb on - strandat 946.342 kb on + strand, within Pf6N2E2_813at 946.343 kb on - strand, within Pf6N2E2_813at 946.527 kb on + strand, within Pf6N2E2_813at 946.527 kb on + strand, within Pf6N2E2_813at 946.527 kb on + strand, within Pf6N2E2_813at 946.527 kb on + strand, within Pf6N2E2_813at 946.527 kb on + strand, within Pf6N2E2_813at 946.627 kb on + strandat 946.628 kb on - strandat 946.628 kb on - strandat 946.628 kb on - strandat 946.628 kb on - strandat 946.628 kb on - strandat 946.628 kb on - strandat 946.628 kb on - strandat 946.690 kb on + strandat 946.690 kb on + strandat 946.690 kb on + strandat 946.691 kb on - strandat 946.724 kb on - strandat 946.737 kb on + strandat 946.737 kb on + strandat 946.738 kb on - strandat 946.739 kb on + strandat 946.740 kb on - strandat 946.742 kb on + strandat 946.775 kb on + strandat 946.776 kb on - strandat 946.782 kb on + strandat 946.819 kb on + strand, within Pf6N2E2_814at 946.819 kb on + strand, within Pf6N2E2_814at 946.819 kb on + strand, within Pf6N2E2_814at 946.819 kb on + strand, within Pf6N2E2_814at 946.820 kb on - strand, within Pf6N2E2_814at 946.820 kb on - strand, within Pf6N2E2_814at 946.820 kb on - strand, within Pf6N2E2_814at 946.820 kb on - strand, within Pf6N2E2_814at 946.944 kb on + strandat 946.944 kb on + strandat 946.944 kb on + strandat 947.005 kb on + strandat 947.005 kb on + strandat 947.005 kb on + strandat 947.006 kb on - strandat 947.146 kb on + strandat 947.184 kb on + strandat 947.185 kb on - strandat 947.185 kb on - strandat 947.240 kb on + strand, within Pf6N2E2_815at 947.240 kb on + strand, within Pf6N2E2_815at 947.240 kb on + strand, within Pf6N2E2_815at 947.241 kb on - strand, within Pf6N2E2_815at 947.241 kb on - strand, within Pf6N2E2_815at 947.241 kb on - strand, within Pf6N2E2_815at 947.241 kb on - strand, within Pf6N2E2_815at 947.270 kb on - strand, within Pf6N2E2_815at 947.270 kb on - strand, within Pf6N2E2_815at 947.458 kb on + strand, within Pf6N2E2_815at 947.458 kb on + strand, within Pf6N2E2_815at 947.459 kb on - strand, within Pf6N2E2_815at 947.497 kb on + strand, within Pf6N2E2_815at 947.498 kb on - strand, within Pf6N2E2_815at 947.498 kb on - strand, within Pf6N2E2_815at 947.536 kb on + strand, within Pf6N2E2_815at 947.536 kb on + strand, within Pf6N2E2_815at 947.537 kb on - strand, within Pf6N2E2_815at 947.537 kb on - strand, within Pf6N2E2_815at 947.537 kb on - strand, within Pf6N2E2_815at 947.537 kb on - strand, within Pf6N2E2_815at 947.668 kb on - strand, within Pf6N2E2_815at 947.668 kb on - strand, within Pf6N2E2_815at 947.689 kb on + strand, within Pf6N2E2_815at 947.690 kb on - strand, within Pf6N2E2_815at 947.716 kb on + strand, within Pf6N2E2_815at 947.758 kb on + strand, within Pf6N2E2_815at 947.758 kb on + strand, within Pf6N2E2_815at 947.759 kb on - strand, within Pf6N2E2_815at 947.759 kb on - strand, within Pf6N2E2_815at 947.814 kb on + strand, within Pf6N2E2_815at 947.815 kb on - strand, within Pf6N2E2_815at 947.864 kb on + strand, within Pf6N2E2_815at 948.033 kb on + strand, within Pf6N2E2_815at 948.481 kb on + strand, within Pf6N2E2_815at 948.481 kb on + strand, within Pf6N2E2_815at 948.520 kb on - strand, within Pf6N2E2_815at 948.659 kb on + strand, within Pf6N2E2_815at 948.685 kb on + strand, within Pf6N2E2_815at 948.685 kb on + strand, within Pf6N2E2_815at 948.685 kb on + strand, within Pf6N2E2_815at 948.686 kb on - strand, within Pf6N2E2_815at 948.686 kb on - strand, within Pf6N2E2_815at 949.002 kb on + strand, within Pf6N2E2_815at 949.024 kb on + strand, within Pf6N2E2_815at 949.024 kb on + strand, within Pf6N2E2_815at 949.025 kb on - strand, within Pf6N2E2_815at 949.025 kb on - strand, within Pf6N2E2_815at 949.793 kb on - strand, within Pf6N2E2_815at 949.840 kb on + strand, within Pf6N2E2_815at 949.841 kb on - strand, within Pf6N2E2_815at 949.841 kb on - strand, within Pf6N2E2_815at 950.150 kb on + strandat 950.150 kb on + strandat 950.150 kb on + strandat 950.150 kb on + strandat 950.150 kb on + strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.151 kb on - strandat 950.227 kb on - strandat 950.427 kb on + strandat 950.427 kb on + strandat 950.428 kb on - strandat 950.428 kb on - strandat 950.433 kb on + strandat 950.658 kb on + strand, within Pf6N2E2_816at 950.659 kb on - strand, within Pf6N2E2_816at 950.709 kb on + strandat 950.710 kb on - strand, within Pf6N2E2_816at 950.764 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.835 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.839 kb on - strand, within Pf6N2E2_816at 950.887 kb on - strand, within Pf6N2E2_816at 950.887 kb on - strand, within Pf6N2E2_816at 950.887 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 950.890 kb on - strand, within Pf6N2E2_816at 951.019 kb on + strandat 951.022 kb on - strand, within Pf6N2E2_816at 951.022 kb on - strand, within Pf6N2E2_816at 951.022 kb on - strand, within Pf6N2E2_816at 951.022 kb on - strand, within Pf6N2E2_816at 951.315 kb on + strand, within Pf6N2E2_817at 951.316 kb on - strand, within Pf6N2E2_817

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Per-strain Table

Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M
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946,138 + -2.2
946,138 + +0.7
946,138 + +0.9
946,138 + +0.9
946,139 - +1.9
946,342 + Pf6N2E2_813 0.28 +3.1
946,343 - Pf6N2E2_813 0.28 +3.6
946,527 + Pf6N2E2_813 0.89 +2.3
946,527 + Pf6N2E2_813 0.89 +2.0
946,527 + Pf6N2E2_813 0.89 +2.9
946,527 + Pf6N2E2_813 0.89 +2.5
946,527 + Pf6N2E2_813 0.89 +2.9
946,627 + +2.8
946,628 - +2.8
946,628 - +3.3
946,628 - +4.2
946,628 - +5.3
946,628 - +3.7
946,628 - +2.0
946,628 - +3.7
946,690 + +2.9
946,690 + +2.3
946,690 + +1.6
946,691 - +1.0
946,724 - +1.8
946,737 + +2.8
946,737 + +0.7
946,738 - +1.0
946,739 + +2.5
946,740 - +1.4
946,742 + +2.8
946,775 + -1.2
946,776 - -0.5
946,782 + +1.8
946,819 + Pf6N2E2_814 0.31 -0.3
946,819 + Pf6N2E2_814 0.31 +1.3
946,819 + Pf6N2E2_814 0.31 -0.0
946,819 + Pf6N2E2_814 0.31 +0.2
946,820 - Pf6N2E2_814 0.32 -1.6
946,820 - Pf6N2E2_814 0.32 +0.4
946,820 - Pf6N2E2_814 0.32 -1.3
946,820 - Pf6N2E2_814 0.32 +0.3
946,944 + +2.3
946,944 + +1.5
946,944 + +2.2
947,005 + +0.4
947,005 + +0.5
947,005 + +0.4
947,006 - -1.6
947,146 + +1.1
947,184 + +1.7
947,185 - +0.2
947,185 - +1.7
947,240 + Pf6N2E2_815 0.10 -0.0
947,240 + Pf6N2E2_815 0.10 +0.7
947,240 + Pf6N2E2_815 0.10 +0.3
947,241 - Pf6N2E2_815 0.10 +0.9
947,241 - Pf6N2E2_815 0.10 -0.3
947,241 - Pf6N2E2_815 0.10 +0.1
947,241 - Pf6N2E2_815 0.10 -0.3
947,270 - Pf6N2E2_815 0.11 -0.0
947,270 - Pf6N2E2_815 0.11 +1.0
947,458 + Pf6N2E2_815 0.16 -0.4
947,458 + Pf6N2E2_815 0.16 +0.5
947,459 - Pf6N2E2_815 0.16 +1.1
947,497 + Pf6N2E2_815 0.17 -0.8
947,498 - Pf6N2E2_815 0.18 +1.0
947,498 - Pf6N2E2_815 0.18 +0.2
947,536 + Pf6N2E2_815 0.19 +2.0
947,536 + Pf6N2E2_815 0.19 +0.7
947,537 - Pf6N2E2_815 0.19 +0.1
947,537 - Pf6N2E2_815 0.19 +1.4
947,537 - Pf6N2E2_815 0.19 +1.3
947,537 - Pf6N2E2_815 0.19 +1.5
947,668 - Pf6N2E2_815 0.22 -0.4
947,668 - Pf6N2E2_815 0.22 -1.3
947,689 + Pf6N2E2_815 0.23 +1.5
947,690 - Pf6N2E2_815 0.23 +1.4
947,716 + Pf6N2E2_815 0.24 +0.2
947,758 + Pf6N2E2_815 0.25 +0.9
947,758 + Pf6N2E2_815 0.25 +0.6
947,759 - Pf6N2E2_815 0.25 +2.0
947,759 - Pf6N2E2_815 0.25 +1.0
947,814 + Pf6N2E2_815 0.27 +1.8
947,815 - Pf6N2E2_815 0.27 +2.3
947,864 + Pf6N2E2_815 0.28 +1.3
948,033 + Pf6N2E2_815 0.33 +2.1
948,481 + Pf6N2E2_815 0.46 -0.2
948,481 + Pf6N2E2_815 0.46 +2.7
948,520 - Pf6N2E2_815 0.47 +1.5
948,659 + Pf6N2E2_815 0.51 +0.7
948,685 + Pf6N2E2_815 0.52 -0.5
948,685 + Pf6N2E2_815 0.52 -1.3
948,685 + Pf6N2E2_815 0.52 -0.0
948,686 - Pf6N2E2_815 0.52 +0.7
948,686 - Pf6N2E2_815 0.52 +1.4
949,002 + Pf6N2E2_815 0.61 -0.9
949,024 + Pf6N2E2_815 0.61 -1.6
949,024 + Pf6N2E2_815 0.61 -0.3
949,025 - Pf6N2E2_815 0.61 +0.7
949,025 - Pf6N2E2_815 0.61 +1.1
949,793 - Pf6N2E2_815 0.83 +0.8
949,840 + Pf6N2E2_815 0.85 -0.6
949,841 - Pf6N2E2_815 0.85 +0.1
949,841 - Pf6N2E2_815 0.85 -0.5
950,150 + -2.5
950,150 + -2.4
950,150 + -1.4
950,150 + -1.5
950,150 + -1.3
950,151 - +1.0
950,151 - +1.1
950,151 - +0.9
950,151 - +1.8
950,151 - -0.5
950,227 - -0.0
950,427 + -0.5
950,427 + -1.0
950,428 - -1.9
950,428 - -0.5
950,433 + -0.5
950,658 + Pf6N2E2_816 0.35 -1.9
950,659 - Pf6N2E2_816 0.35 -1.0
950,709 + +0.5
950,710 - Pf6N2E2_816 0.42 -0.1
950,764 - Pf6N2E2_816 0.48 -1.9
950,835 - Pf6N2E2_816 0.57 +1.0
950,835 - Pf6N2E2_816 0.57 +0.8
950,835 - Pf6N2E2_816 0.57 +0.3
950,835 - Pf6N2E2_816 0.57 +1.1
950,839 - Pf6N2E2_816 0.57 +1.0
950,839 - Pf6N2E2_816 0.57 -0.0
950,839 - Pf6N2E2_816 0.57 -0.0
950,839 - Pf6N2E2_816 0.57 +0.3
950,839 - Pf6N2E2_816 0.57 +0.0
950,839 - Pf6N2E2_816 0.57 -1.1
950,887 - Pf6N2E2_816 0.63 +0.3
950,887 - Pf6N2E2_816 0.63 -0.6
950,887 - Pf6N2E2_816 0.63 -1.0
950,890 - Pf6N2E2_816 0.64 +0.5
950,890 - Pf6N2E2_816 0.64 -0.1
950,890 - Pf6N2E2_816 0.64 -1.3
950,890 - Pf6N2E2_816 0.64 +0.3
950,890 - Pf6N2E2_816 0.64 -0.0
950,890 - Pf6N2E2_816 0.64 +1.0
950,890 - Pf6N2E2_816 0.64 +2.1
950,890 - Pf6N2E2_816 0.64 -0.8
951,019 + +1.5
951,022 - Pf6N2E2_816 0.80 +1.2
951,022 - Pf6N2E2_816 0.80 -1.0
951,022 - Pf6N2E2_816 0.80 +1.8
951,022 - Pf6N2E2_816 0.80 +1.5
951,315 + Pf6N2E2_817 0.23 -0.5
951,316 - Pf6N2E2_817 0.23 +0.4

Or see this region's nucleotide sequence