Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_597 and Pf6N2E2_598 are separated by 16 nucleotides Pf6N2E2_598 and Pf6N2E2_599 are separated by 93 nucleotides Pf6N2E2_599 and Pf6N2E2_600 overlap by 1 nucleotides Pf6N2E2_600 and Pf6N2E2_601 overlap by 4 nucleotides
Pf6N2E2_597 - FIG00953564: hypothetical protein, at 673,002 to 673,967
_597
Pf6N2E2_598 - hypothetical protein, at 673,984 to 674,145
_598
Pf6N2E2_599 - NAD kinase (EC 2.7.1.23), at 674,239 to 675,129
_599
Pf6N2E2_600 - Diadenosine tetraphosphatase and related serine/threonine protein phosphatases, at 675,129 to 676,100
_600
Pf6N2E2_601 - GlpG protein (membrane protein of glp regulon), at 676,097 to 676,969
_601
Position (kb) 674
675
676 Strain fitness (log2 ratio) -2
-1
0
1 at 673.339 kb on + strand, within Pf6N2E2_597 at 673.378 kb on - strand, within Pf6N2E2_597 at 673.536 kb on - strand, within Pf6N2E2_597 at 673.555 kb on + strand, within Pf6N2E2_597 at 673.555 kb on + strand, within Pf6N2E2_597 at 673.556 kb on - strand, within Pf6N2E2_597 at 673.556 kb on - strand, within Pf6N2E2_597 at 673.603 kb on - strand, within Pf6N2E2_597 at 673.718 kb on - strand, within Pf6N2E2_597 at 673.718 kb on - strand, within Pf6N2E2_597 at 674.016 kb on - strand, within Pf6N2E2_598 at 674.178 kb on + strand at 674.197 kb on + strand at 675.423 kb on + strand, within Pf6N2E2_600 at 675.423 kb on + strand, within Pf6N2E2_600 at 675.424 kb on - strand at 675.526 kb on - strand, within Pf6N2E2_600 at 675.933 kb on + strand, within Pf6N2E2_600 at 675.988 kb on - strand, within Pf6N2E2_600
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 673,339 + Pf6N2E2_597 0.35 +0.6 673,378 - Pf6N2E2_597 0.39 -2.0 673,536 - Pf6N2E2_597 0.55 +0.7 673,555 + Pf6N2E2_597 0.57 +1.4 673,555 + Pf6N2E2_597 0.57 +0.9 673,556 - Pf6N2E2_597 0.57 +1.0 673,556 - Pf6N2E2_597 0.57 -0.6 673,603 - Pf6N2E2_597 0.62 +0.8 673,718 - Pf6N2E2_597 0.74 +0.0 673,718 - Pf6N2E2_597 0.74 +0.4 674,016 - Pf6N2E2_598 0.20 +1.2 674,178 + +0.5 674,197 + +1.0 675,423 + Pf6N2E2_600 0.30 +0.3 675,423 + Pf6N2E2_600 0.30 -1.6 675,424 - -0.2 675,526 - Pf6N2E2_600 0.41 -0.0 675,933 + Pf6N2E2_600 0.83 +0.3 675,988 - Pf6N2E2_600 0.88 -0.0
Or see this region's nucleotide sequence