Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5383 and Pf6N2E2_5384 are separated by 26 nucleotides Pf6N2E2_5384 and Pf6N2E2_5385 overlap by 4 nucleotides
Pf6N2E2_5383 - Poly(beta-D-mannuronate) C5 epimerase precursor (EC 5.1.3.-), at 6,185,829 to 6,187,418
_5383
Pf6N2E2_5384 - outer membrane protein AlgE, at 6,187,445 to 6,188,944
_5384
Pf6N2E2_5385 - Alginate biosynthesis protein AlgK precursor, at 6,188,941 to 6,190,341
_5385
Position (kb)
6187
6188
6189 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.518 kb on - strand, within Pf6N2E2_5383 at 6186.553 kb on + strand, within Pf6N2E2_5383 at 6186.553 kb on + strand, within Pf6N2E2_5383 at 6186.554 kb on - strand, within Pf6N2E2_5383 at 6186.559 kb on + strand at 6186.559 kb on + strand, within Pf6N2E2_5383 at 6186.560 kb on - strand, within Pf6N2E2_5383 at 6186.649 kb on + strand, within Pf6N2E2_5383 at 6186.650 kb on - strand, within Pf6N2E2_5383 at 6186.659 kb on + strand, within Pf6N2E2_5383 at 6186.659 kb on + strand, within Pf6N2E2_5383 at 6186.660 kb on - strand, within Pf6N2E2_5383 at 6186.660 kb on - strand, within Pf6N2E2_5383 at 6186.677 kb on + strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.678 kb on - strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.721 kb on + strand, within Pf6N2E2_5383 at 6186.722 kb on - strand, within Pf6N2E2_5383 at 6186.722 kb on - strand, within Pf6N2E2_5383 at 6186.955 kb on + strand, within Pf6N2E2_5383 at 6186.955 kb on + strand, within Pf6N2E2_5383 at 6186.982 kb on + strand, within Pf6N2E2_5383 at 6186.982 kb on + strand, within Pf6N2E2_5383 at 6186.982 kb on + strand, within Pf6N2E2_5383 at 6186.983 kb on - strand, within Pf6N2E2_5383 at 6187.021 kb on + strand, within Pf6N2E2_5383 at 6187.021 kb on + strand, within Pf6N2E2_5383 at 6187.022 kb on - strand, within Pf6N2E2_5383 at 6187.195 kb on + strand, within Pf6N2E2_5383 at 6187.196 kb on - strand, within Pf6N2E2_5383 at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.264 kb on + strand at 6187.265 kb on - strand at 6187.265 kb on - strand at 6187.265 kb on - strand at 6187.265 kb on - strand at 6187.409 kb on - strand at 6187.409 kb on - strand at 6187.485 kb on + strand at 6187.486 kb on - strand at 6187.486 kb on - strand at 6187.506 kb on + strand at 6187.609 kb on - strand, within Pf6N2E2_5384 at 6187.609 kb on - strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.779 kb on + strand, within Pf6N2E2_5384 at 6187.780 kb on - strand, within Pf6N2E2_5384 at 6187.941 kb on + strand, within Pf6N2E2_5384 at 6187.962 kb on + strand, within Pf6N2E2_5384 at 6188.130 kb on + strand, within Pf6N2E2_5384 at 6188.131 kb on - strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.251 kb on - strand, within Pf6N2E2_5384 at 6188.698 kb on - strand, within Pf6N2E2_5384 at 6188.730 kb on + strand, within Pf6N2E2_5384 at 6188.731 kb on - strand, within Pf6N2E2_5384 at 6188.731 kb on - strand, within Pf6N2E2_5384 at 6188.944 kb on + strand at 6188.971 kb on + strand at 6189.031 kb on + strand at 6189.031 kb on + strand at 6189.031 kb on + strand at 6189.068 kb on - strand at 6189.143 kb on + strand, within Pf6N2E2_5385 at 6189.143 kb on + strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.266 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.294 kb on - strand, within Pf6N2E2_5385 at 6189.308 kb on + strand, within Pf6N2E2_5385 at 6189.309 kb on - strand, within Pf6N2E2_5385 at 6189.309 kb on - strand, within Pf6N2E2_5385 at 6189.311 kb on + strand, within Pf6N2E2_5385 at 6189.395 kb on + strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.398 kb on + strand, within Pf6N2E2_5385 at 6189.399 kb on - strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.459 kb on - strand, within Pf6N2E2_5385 at 6189.501 kb on - strand, within Pf6N2E2_5385 at 6189.719 kb on + strand, within Pf6N2E2_5385 at 6189.720 kb on - strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.741 kb on - strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 6,186,518 - Pf6N2E2_5383 0.43 +0.5 6,186,518 - Pf6N2E2_5383 0.43 +0.1 6,186,518 - Pf6N2E2_5383 0.43 +0.3 6,186,518 - Pf6N2E2_5383 0.43 +0.2 6,186,553 + Pf6N2E2_5383 0.46 +1.2 6,186,553 + Pf6N2E2_5383 0.46 +1.8 6,186,554 - Pf6N2E2_5383 0.46 -0.4 6,186,559 + +2.2 6,186,559 + Pf6N2E2_5383 0.46 +1.0 6,186,560 - Pf6N2E2_5383 0.46 -0.3 6,186,649 + Pf6N2E2_5383 0.52 +0.2 6,186,650 - Pf6N2E2_5383 0.52 +0.9 6,186,659 + Pf6N2E2_5383 0.52 +0.2 6,186,659 + Pf6N2E2_5383 0.52 +1.2 6,186,660 - Pf6N2E2_5383 0.52 +0.2 6,186,660 - Pf6N2E2_5383 0.52 -0.8 6,186,677 + Pf6N2E2_5383 0.53 +0.2 6,186,678 - Pf6N2E2_5383 0.53 -0.4 6,186,678 - Pf6N2E2_5383 0.53 -0.1 6,186,678 - Pf6N2E2_5383 0.53 +0.3 6,186,678 - Pf6N2E2_5383 0.53 +0.3 6,186,678 - Pf6N2E2_5383 0.53 +1.0 6,186,721 + Pf6N2E2_5383 0.56 +1.1 6,186,721 + Pf6N2E2_5383 0.56 +1.1 6,186,721 + Pf6N2E2_5383 0.56 +1.6 6,186,721 + Pf6N2E2_5383 0.56 -0.2 6,186,721 + Pf6N2E2_5383 0.56 +0.8 6,186,722 - Pf6N2E2_5383 0.56 -1.2 6,186,722 - Pf6N2E2_5383 0.56 -1.5 6,186,955 + Pf6N2E2_5383 0.71 -0.3 6,186,955 + Pf6N2E2_5383 0.71 +0.6 6,186,982 + Pf6N2E2_5383 0.73 +0.4 6,186,982 + Pf6N2E2_5383 0.73 -0.3 6,186,982 + Pf6N2E2_5383 0.73 -0.3 6,186,983 - Pf6N2E2_5383 0.73 -0.8 6,187,021 + Pf6N2E2_5383 0.75 +0.6 6,187,021 + Pf6N2E2_5383 0.75 -0.3 6,187,022 - Pf6N2E2_5383 0.75 -0.3 6,187,195 + Pf6N2E2_5383 0.86 +0.6 6,187,196 - Pf6N2E2_5383 0.86 -0.4 6,187,264 + +0.6 6,187,264 + -0.8 6,187,264 + +2.2 6,187,264 + +1.0 6,187,264 + -0.0 6,187,264 + +0.1 6,187,265 - -0.7 6,187,265 - +0.9 6,187,265 - -1.4 6,187,265 - -1.2 6,187,409 - -1.8 6,187,409 - -1.4 6,187,485 + -1.7 6,187,486 - -1.5 6,187,486 - -0.9 6,187,506 + -0.1 6,187,609 - Pf6N2E2_5384 0.11 -1.3 6,187,609 - Pf6N2E2_5384 0.11 -1.6 6,187,779 + Pf6N2E2_5384 0.22 +1.1 6,187,779 + Pf6N2E2_5384 0.22 +1.1 6,187,779 + Pf6N2E2_5384 0.22 +1.5 6,187,779 + Pf6N2E2_5384 0.22 +0.3 6,187,779 + Pf6N2E2_5384 0.22 +0.1 6,187,779 + Pf6N2E2_5384 0.22 +1.6 6,187,780 - Pf6N2E2_5384 0.22 +0.3 6,187,941 + Pf6N2E2_5384 0.33 +1.1 6,187,962 + Pf6N2E2_5384 0.34 -0.5 6,188,130 + Pf6N2E2_5384 0.46 -0.4 6,188,131 - Pf6N2E2_5384 0.46 +0.0 6,188,160 + Pf6N2E2_5384 0.48 +1.2 6,188,160 + Pf6N2E2_5384 0.48 +0.1 6,188,160 + Pf6N2E2_5384 0.48 +0.6 6,188,160 + Pf6N2E2_5384 0.48 +0.7 6,188,251 - Pf6N2E2_5384 0.54 -0.2 6,188,698 - Pf6N2E2_5384 0.84 -0.7 6,188,730 + Pf6N2E2_5384 0.86 +0.9 6,188,731 - Pf6N2E2_5384 0.86 +0.6 6,188,731 - Pf6N2E2_5384 0.86 -0.6 6,188,944 + +0.6 6,188,971 + +0.5 6,189,031 + +0.6 6,189,031 + -1.4 6,189,031 + +0.8 6,189,068 - +0.6 6,189,143 + Pf6N2E2_5385 0.14 +1.0 6,189,143 + Pf6N2E2_5385 0.14 +0.9 6,189,144 - Pf6N2E2_5385 0.14 +1.1 6,189,144 - Pf6N2E2_5385 0.14 +0.0 6,189,144 - Pf6N2E2_5385 0.14 +0.1 6,189,144 - Pf6N2E2_5385 0.14 -0.1 6,189,144 - Pf6N2E2_5385 0.14 +0.4 6,189,144 - Pf6N2E2_5385 0.14 +0.3 6,189,144 - Pf6N2E2_5385 0.14 -0.6 6,189,144 - Pf6N2E2_5385 0.14 +0.2 6,189,266 + Pf6N2E2_5385 0.23 +0.7 6,189,293 + Pf6N2E2_5385 0.25 -0.1 6,189,293 + Pf6N2E2_5385 0.25 +1.0 6,189,293 + Pf6N2E2_5385 0.25 +0.2 6,189,293 + Pf6N2E2_5385 0.25 +4.6 6,189,294 - Pf6N2E2_5385 0.25 -0.8 6,189,308 + Pf6N2E2_5385 0.26 +0.6 6,189,309 - Pf6N2E2_5385 0.26 -0.5 6,189,309 - Pf6N2E2_5385 0.26 +1.2 6,189,311 + Pf6N2E2_5385 0.26 +2.6 6,189,395 + Pf6N2E2_5385 0.32 +0.4 6,189,396 - Pf6N2E2_5385 0.32 -1.1 6,189,396 - Pf6N2E2_5385 0.32 +0.6 6,189,396 - Pf6N2E2_5385 0.32 -2.1 6,189,396 - Pf6N2E2_5385 0.32 -2.0 6,189,398 + Pf6N2E2_5385 0.33 -0.0 6,189,399 - Pf6N2E2_5385 0.33 -0.6 6,189,458 + Pf6N2E2_5385 0.37 +1.0 6,189,458 + Pf6N2E2_5385 0.37 -0.9 6,189,459 - Pf6N2E2_5385 0.37 -0.7 6,189,501 - Pf6N2E2_5385 0.40 -1.4 6,189,719 + Pf6N2E2_5385 0.56 +1.4 6,189,720 - Pf6N2E2_5385 0.56 -1.4 6,189,740 + Pf6N2E2_5385 0.57 +0.7 6,189,740 + Pf6N2E2_5385 0.57 +1.8 6,189,740 + Pf6N2E2_5385 0.57 -0.5 6,189,741 - Pf6N2E2_5385 0.57 +0.1 6,189,806 + Pf6N2E2_5385 0.62 +1.6 6,189,806 + Pf6N2E2_5385 0.62 -0.5 6,189,807 - Pf6N2E2_5385 0.62 +1.5 6,189,807 - Pf6N2E2_5385 0.62 -0.1 6,189,807 - Pf6N2E2_5385 0.62 -1.9 6,189,807 - Pf6N2E2_5385 0.62 -0.8 6,189,929 + Pf6N2E2_5385 0.71 +1.6 6,189,929 + Pf6N2E2_5385 0.71 -0.3 6,189,929 + Pf6N2E2_5385 0.71 +1.3 6,189,930 - Pf6N2E2_5385 0.71 -1.9 6,189,930 - Pf6N2E2_5385 0.71 -1.5
Or see this region's nucleotide sequence