Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_436 and Pf6N2E2_437 are separated by 88 nucleotides Pf6N2E2_437 and Pf6N2E2_438 overlap by 11 nucleotides Pf6N2E2_438 and Pf6N2E2_439 are separated by 301 nucleotides Pf6N2E2_439 and Pf6N2E2_440 are separated by 30 nucleotides
Pf6N2E2_436 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 502,012 to 503,085
_436
Pf6N2E2_437 - Alkylated DNA repair protein AlkB, at 503,174 to 503,851
_437
Pf6N2E2_438 - hypothetical protein, at 503,841 to 504,569
_438
Pf6N2E2_439 - FIG00958580: hypothetical protein, at 504,871 to 505,041
_439
Pf6N2E2_440 - FIG00954920: hypothetical protein, at 505,072 to 505,374
_440
Position (kb) 503
504
505 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.984 kb on + strand at 502.991 kb on + strand at 502.991 kb on + strand at 502.992 kb on - strand at 503.092 kb on + strand at 503.092 kb on + strand at 503.092 kb on + strand at 503.092 kb on + strand at 503.093 kb on - strand at 503.116 kb on - strand at 503.116 kb on - strand at 503.417 kb on - strand, within Pf6N2E2_437 at 503.417 kb on - strand, within Pf6N2E2_437 at 503.452 kb on - strand, within Pf6N2E2_437 at 503.581 kb on + strand, within Pf6N2E2_437 at 503.583 kb on + strand, within Pf6N2E2_437 at 503.584 kb on - strand, within Pf6N2E2_437 at 503.584 kb on - strand, within Pf6N2E2_437 at 503.584 kb on - strand, within Pf6N2E2_437 at 503.647 kb on + strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.648 kb on - strand, within Pf6N2E2_437 at 503.650 kb on + strand, within Pf6N2E2_437 at 503.650 kb on + strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.651 kb on - strand, within Pf6N2E2_437 at 503.882 kb on + strand at 504.059 kb on + strand, within Pf6N2E2_438 at 504.122 kb on - strand, within Pf6N2E2_438 at 504.145 kb on + strand, within Pf6N2E2_438 at 504.146 kb on - strand, within Pf6N2E2_438 at 504.146 kb on - strand, within Pf6N2E2_438 at 504.161 kb on - strand, within Pf6N2E2_438 at 504.299 kb on - strand, within Pf6N2E2_438 at 504.355 kb on + strand, within Pf6N2E2_438 at 504.528 kb on + strand at 504.528 kb on + strand at 504.529 kb on - strand at 504.529 kb on - strand at 504.856 kb on + strand at 504.857 kb on - strand at 505.010 kb on + strand, within Pf6N2E2_439 at 505.010 kb on + strand, within Pf6N2E2_439 at 505.010 kb on + strand, within Pf6N2E2_439 at 505.010 kb on + strand, within Pf6N2E2_439 at 505.011 kb on - strand, within Pf6N2E2_439 at 505.011 kb on - strand, within Pf6N2E2_439 at 505.022 kb on + strand, within Pf6N2E2_439 at 505.022 kb on + strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.023 kb on - strand, within Pf6N2E2_439 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.242 kb on - strand, within Pf6N2E2_440 at 505.269 kb on + strand, within Pf6N2E2_440 at 505.440 kb on + strand at 505.441 kb on - strand at 505.457 kb on - strand at 505.457 kb on - strand at 505.457 kb on - strand at 505.459 kb on + strand at 505.491 kb on - strand at 505.491 kb on - strand at 505.511 kb on + strand at 505.512 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 502,967 + Pf6N2E2_436 0.89 +2.8 502,967 + Pf6N2E2_436 0.89 -1.0 502,967 + Pf6N2E2_436 0.89 -0.3 502,967 + Pf6N2E2_436 0.89 +0.9 502,967 + Pf6N2E2_436 0.89 -1.1 502,967 + Pf6N2E2_436 0.89 +0.5 502,967 + Pf6N2E2_436 0.89 +0.5 502,968 - Pf6N2E2_436 0.89 -1.0 502,968 - Pf6N2E2_436 0.89 -0.0 502,968 - Pf6N2E2_436 0.89 +0.3 502,984 + +0.3 502,991 + +0.7 502,991 + -1.3 502,992 - -1.0 503,092 + +0.2 503,092 + +0.4 503,092 + -0.7 503,092 + -0.9 503,093 - -0.6 503,116 - -0.5 503,116 - -1.0 503,417 - Pf6N2E2_437 0.36 -0.3 503,417 - Pf6N2E2_437 0.36 -0.6 503,452 - Pf6N2E2_437 0.41 -1.4 503,581 + Pf6N2E2_437 0.60 -0.1 503,583 + Pf6N2E2_437 0.60 +1.4 503,584 - Pf6N2E2_437 0.60 -1.0 503,584 - Pf6N2E2_437 0.60 +0.1 503,584 - Pf6N2E2_437 0.60 +0.2 503,647 + Pf6N2E2_437 0.70 -0.5 503,648 - Pf6N2E2_437 0.70 -0.2 503,648 - Pf6N2E2_437 0.70 -0.1 503,648 - Pf6N2E2_437 0.70 -2.3 503,648 - Pf6N2E2_437 0.70 -0.4 503,650 + Pf6N2E2_437 0.70 +0.1 503,650 + Pf6N2E2_437 0.70 -1.3 503,651 - Pf6N2E2_437 0.70 -0.2 503,651 - Pf6N2E2_437 0.70 -0.4 503,651 - Pf6N2E2_437 0.70 +1.5 503,651 - Pf6N2E2_437 0.70 -0.0 503,882 + +0.4 504,059 + Pf6N2E2_438 0.30 +1.8 504,122 - Pf6N2E2_438 0.39 +0.8 504,145 + Pf6N2E2_438 0.42 +0.9 504,146 - Pf6N2E2_438 0.42 -0.1 504,146 - Pf6N2E2_438 0.42 +0.3 504,161 - Pf6N2E2_438 0.44 +0.6 504,299 - Pf6N2E2_438 0.63 +0.1 504,355 + Pf6N2E2_438 0.71 -0.5 504,528 + -1.1 504,528 + -2.3 504,529 - +0.8 504,529 - +0.4 504,856 + -0.3 504,857 - +1.2 505,010 + Pf6N2E2_439 0.81 -1.0 505,010 + Pf6N2E2_439 0.81 -0.4 505,010 + Pf6N2E2_439 0.81 +1.2 505,010 + Pf6N2E2_439 0.81 +0.7 505,011 - Pf6N2E2_439 0.82 -0.4 505,011 - Pf6N2E2_439 0.82 -0.2 505,022 + Pf6N2E2_439 0.88 -0.3 505,022 + Pf6N2E2_439 0.88 +1.2 505,023 - Pf6N2E2_439 0.89 +0.7 505,023 - Pf6N2E2_439 0.89 +0.1 505,023 - Pf6N2E2_439 0.89 -1.3 505,023 - Pf6N2E2_439 0.89 +0.5 505,242 - Pf6N2E2_440 0.56 +2.7 505,242 - Pf6N2E2_440 0.56 +0.5 505,242 - Pf6N2E2_440 0.56 +1.1 505,242 - Pf6N2E2_440 0.56 -0.8 505,269 + Pf6N2E2_440 0.65 -1.3 505,440 + +1.3 505,441 - -2.5 505,457 - +2.6 505,457 - +0.0 505,457 - +0.0 505,459 + -0.3 505,491 - +1.1 505,491 - -0.9 505,511 + +0.0 505,512 - +0.7
Or see this region's nucleotide sequence