Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_421 and Pf6N2E2_422 are separated by 551 nucleotides Pf6N2E2_422 and Pf6N2E2_423 are separated by 20 nucleotides Pf6N2E2_423 and Pf6N2E2_424 are separated by 129 nucleotides
Pf6N2E2_421 - Methyl-accepting chemotaxis protein, at 488,862 to 490,013
_421
Pf6N2E2_422 - Na+ driven multidrug efflux pump, at 490,565 to 491,863
_422
Pf6N2E2_423 - Putative phosphatase YfbT, at 491,884 to 492,588
_423
Pf6N2E2_424 - Decarboxylase family protein, at 492,718 to 494,091
_424
Position (kb) 490
491
492 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 489.903 kb on + strand at 489.904 kb on - strand at 490.106 kb on + strand at 490.106 kb on + strand at 490.106 kb on + strand at 490.107 kb on - strand at 490.107 kb on - strand at 490.107 kb on - strand at 490.107 kb on - strand at 490.117 kb on - strand at 490.117 kb on - strand at 490.178 kb on + strand at 490.179 kb on - strand at 490.190 kb on + strand at 490.190 kb on + strand at 490.190 kb on + strand at 490.197 kb on + strand at 490.198 kb on - strand at 490.213 kb on - strand at 490.262 kb on + strand at 490.263 kb on - strand at 490.544 kb on + strand at 490.545 kb on - strand at 490.545 kb on - strand at 490.616 kb on + strand at 490.616 kb on + strand at 490.616 kb on + strand at 490.616 kb on + strand at 490.617 kb on - strand at 490.617 kb on - strand at 490.617 kb on - strand at 490.617 kb on - strand at 490.617 kb on - strand at 490.904 kb on + strand, within Pf6N2E2_422 at 490.904 kb on + strand, within Pf6N2E2_422 at 490.905 kb on - strand, within Pf6N2E2_422 at 490.945 kb on - strand, within Pf6N2E2_422 at 490.945 kb on - strand, within Pf6N2E2_422 at 490.945 kb on - strand, within Pf6N2E2_422 at 491.241 kb on + strand, within Pf6N2E2_422 at 491.241 kb on + strand, within Pf6N2E2_422 at 491.242 kb on - strand, within Pf6N2E2_422 at 491.312 kb on - strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.366 kb on + strand, within Pf6N2E2_422 at 491.367 kb on - strand, within Pf6N2E2_422 at 491.367 kb on - strand, within Pf6N2E2_422 at 491.367 kb on - strand, within Pf6N2E2_422 at 491.367 kb on - strand at 491.495 kb on - strand, within Pf6N2E2_422 at 491.519 kb on + strand, within Pf6N2E2_422 at 491.519 kb on + strand, within Pf6N2E2_422 at 491.686 kb on + strand, within Pf6N2E2_422 at 491.687 kb on - strand, within Pf6N2E2_422 at 491.729 kb on + strand, within Pf6N2E2_422 at 491.730 kb on - strand, within Pf6N2E2_422 at 491.786 kb on + strand at 491.787 kb on - strand at 491.817 kb on - strand at 491.819 kb on + strand at 491.820 kb on - strand at 491.861 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.877 kb on + strand at 491.878 kb on - strand at 491.878 kb on - strand at 491.878 kb on - strand at 491.878 kb on - strand at 492.279 kb on + strand, within Pf6N2E2_423 at 492.486 kb on + strand, within Pf6N2E2_423 at 492.526 kb on + strand at 492.526 kb on + strand at 492.527 kb on - strand at 492.527 kb on - strand at 492.527 kb on - strand at 492.584 kb on + strand at 492.584 kb on + strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.585 kb on - strand at 492.660 kb on + strand at 492.714 kb on + strand at 492.715 kb on - strand at 492.746 kb on + strand at 492.746 kb on + strand at 492.747 kb on - strand at 492.747 kb on - strand at 492.747 kb on - strand at 492.747 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 489,903 + +0.9 489,904 - +0.0 490,106 + +0.9 490,106 + +1.0 490,106 + -1.1 490,107 - +0.0 490,107 - -1.7 490,107 - +1.2 490,107 - -2.8 490,117 - -0.3 490,117 - -0.6 490,178 + -1.1 490,179 - +0.3 490,190 + -1.9 490,190 + -0.0 490,190 + +0.5 490,197 + -2.1 490,198 - +2.7 490,213 - +0.9 490,262 + -0.2 490,263 - -0.0 490,544 + -1.0 490,545 - +0.2 490,545 - -0.1 490,616 + -0.6 490,616 + -0.3 490,616 + +0.1 490,616 + -1.3 490,617 - -0.3 490,617 - -0.7 490,617 - -0.6 490,617 - +1.3 490,617 - -1.1 490,904 + Pf6N2E2_422 0.26 -1.2 490,904 + Pf6N2E2_422 0.26 -0.1 490,905 - Pf6N2E2_422 0.26 +1.1 490,945 - Pf6N2E2_422 0.29 -0.3 490,945 - Pf6N2E2_422 0.29 -0.5 490,945 - Pf6N2E2_422 0.29 -1.6 491,241 + Pf6N2E2_422 0.52 +0.3 491,241 + Pf6N2E2_422 0.52 -0.9 491,242 - Pf6N2E2_422 0.52 -1.1 491,312 - Pf6N2E2_422 0.58 -2.4 491,366 + Pf6N2E2_422 0.62 -0.6 491,366 + Pf6N2E2_422 0.62 -1.0 491,366 + Pf6N2E2_422 0.62 -0.6 491,366 + Pf6N2E2_422 0.62 -0.8 491,366 + Pf6N2E2_422 0.62 +0.4 491,366 + Pf6N2E2_422 0.62 -0.1 491,366 + Pf6N2E2_422 0.62 -0.9 491,366 + Pf6N2E2_422 0.62 +0.3 491,366 + Pf6N2E2_422 0.62 +0.7 491,366 + Pf6N2E2_422 0.62 +0.6 491,367 - Pf6N2E2_422 0.62 -0.0 491,367 - Pf6N2E2_422 0.62 +0.5 491,367 - Pf6N2E2_422 0.62 -1.5 491,367 - +1.7 491,495 - Pf6N2E2_422 0.72 -1.6 491,519 + Pf6N2E2_422 0.73 -2.0 491,519 + Pf6N2E2_422 0.73 -2.1 491,686 + Pf6N2E2_422 0.86 -1.3 491,687 - Pf6N2E2_422 0.86 -0.1 491,729 + Pf6N2E2_422 0.90 -1.1 491,730 - Pf6N2E2_422 0.90 +1.0 491,786 + +1.2 491,787 - +0.7 491,817 - -0.9 491,819 + +1.1 491,820 - +1.3 491,861 + -0.5 491,877 + -0.1 491,877 + +0.1 491,877 + +0.3 491,877 + +0.6 491,878 - -1.6 491,878 - +0.4 491,878 - +1.7 491,878 - +0.9 492,279 + Pf6N2E2_423 0.56 +0.2 492,486 + Pf6N2E2_423 0.85 +2.0 492,526 + +0.2 492,526 + +0.4 492,527 - +0.1 492,527 - -0.1 492,527 - +1.3 492,584 + -0.6 492,584 + -0.3 492,585 - +0.3 492,585 - +1.0 492,585 - +0.2 492,585 - +0.7 492,660 + +0.1 492,714 + +1.3 492,715 - +0.1 492,746 + +0.1 492,746 + +1.1 492,747 - +1.8 492,747 - -0.8 492,747 - -1.2 492,747 - +0.6
Or see this region's nucleotide sequence