Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3774 and Pf6N2E2_3775 are separated by 61 nucleotides Pf6N2E2_3775 and Pf6N2E2_3776 are separated by 162 nucleotides Pf6N2E2_3776 and Pf6N2E2_3777 are separated by 10 nucleotides Pf6N2E2_3777 and Pf6N2E2_3778 are separated by 262 nucleotides
Pf6N2E2_3774 - Polyhydroxyalkanoic acid synthase, at 4,397,875 to 4,399,557
_3774
Pf6N2E2_3775 - Transcriptional regulator PhaD, at 4,399,619 to 4,400,239
_3775
Pf6N2E2_3776 - Polyhydroxyalkanoate granule-associated protein PhaF, at 4,400,402 to 4,401,283
_3776
Pf6N2E2_3777 - polyhydroxyalkanoate granule-associated protein PhaI, at 4,401,294 to 4,401,716
_3777
Pf6N2E2_3778 - FIG00954300: hypothetical protein, at 4,401,979 to 4,402,254
_3778
Position (kb)
4400
4401
4402 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4399.402 kb on + strand at 4399.402 kb on + strand at 4399.510 kb on + strand at 4399.511 kb on - strand at 4399.511 kb on - strand at 4399.511 kb on - strand at 4399.511 kb on - strand at 4399.540 kb on + strand at 4399.540 kb on + strand at 4399.540 kb on + strand at 4399.540 kb on + strand at 4399.540 kb on + strand at 4399.540 kb on + strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.541 kb on - strand at 4399.607 kb on + strand at 4399.607 kb on + strand at 4399.608 kb on - strand at 4399.736 kb on + strand, within Pf6N2E2_3775 at 4399.736 kb on + strand, within Pf6N2E2_3775 at 4399.736 kb on + strand, within Pf6N2E2_3775 at 4399.736 kb on + strand, within Pf6N2E2_3775 at 4399.737 kb on - strand, within Pf6N2E2_3775 at 4399.737 kb on - strand, within Pf6N2E2_3775 at 4399.737 kb on - strand, within Pf6N2E2_3775 at 4399.737 kb on - strand, within Pf6N2E2_3775 at 4399.740 kb on - strand, within Pf6N2E2_3775 at 4399.893 kb on - strand, within Pf6N2E2_3775 at 4400.050 kb on - strand, within Pf6N2E2_3775 at 4400.099 kb on + strand, within Pf6N2E2_3775 at 4400.100 kb on - strand, within Pf6N2E2_3775 at 4400.100 kb on - strand, within Pf6N2E2_3775 at 4400.219 kb on + strand at 4400.238 kb on - strand at 4400.417 kb on - strand at 4400.417 kb on - strand at 4400.417 kb on - strand at 4401.195 kb on - strand at 4401.223 kb on - strand at 4401.223 kb on - strand at 4401.303 kb on - strand at 4401.372 kb on - strand, within Pf6N2E2_3777 at 4401.372 kb on - strand, within Pf6N2E2_3777 at 4401.372 kb on - strand, within Pf6N2E2_3777 at 4401.445 kb on - strand, within Pf6N2E2_3777 at 4401.445 kb on - strand, within Pf6N2E2_3777 at 4401.568 kb on - strand, within Pf6N2E2_3777 at 4401.568 kb on - strand, within Pf6N2E2_3777 at 4401.650 kb on - strand, within Pf6N2E2_3777 at 4401.650 kb on - strand, within Pf6N2E2_3777 at 4401.650 kb on - strand, within Pf6N2E2_3777 at 4401.780 kb on + strand at 4401.780 kb on + strand at 4401.781 kb on - strand at 4401.789 kb on + strand at 4401.789 kb on + strand at 4401.789 kb on + strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.790 kb on - strand at 4401.794 kb on - strand at 4401.807 kb on + strand at 4401.807 kb on + strand at 4401.808 kb on - strand at 4401.901 kb on - strand at 4401.908 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4401.960 kb on - strand at 4402.160 kb on + strand, within Pf6N2E2_3778 at 4402.160 kb on + strand, within Pf6N2E2_3778 at 4402.161 kb on - strand, within Pf6N2E2_3778 at 4402.165 kb on - strand, within Pf6N2E2_3778 at 4402.243 kb on + strand at 4402.245 kb on + strand at 4402.245 kb on + strand at 4402.245 kb on + strand at 4402.246 kb on - strand at 4402.246 kb on - strand at 4402.246 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 4,399,402 + +0.8 4,399,402 + -0.5 4,399,510 + -0.8 4,399,511 - +0.6 4,399,511 - +0.6 4,399,511 - -0.6 4,399,511 - +0.1 4,399,540 + +0.1 4,399,540 + +0.3 4,399,540 + +1.0 4,399,540 + -1.7 4,399,540 + +1.3 4,399,540 + +0.4 4,399,541 - -0.4 4,399,541 - +0.7 4,399,541 - -0.8 4,399,541 - -1.2 4,399,541 - -1.0 4,399,541 - -0.0 4,399,541 - +0.4 4,399,541 - +1.8 4,399,541 - -0.3 4,399,541 - +0.1 4,399,541 - +1.6 4,399,541 - +1.2 4,399,541 - -0.8 4,399,541 - +0.3 4,399,607 + +0.2 4,399,607 + -0.6 4,399,608 - -1.4 4,399,736 + Pf6N2E2_3775 0.19 -1.0 4,399,736 + Pf6N2E2_3775 0.19 -0.2 4,399,736 + Pf6N2E2_3775 0.19 -0.6 4,399,736 + Pf6N2E2_3775 0.19 -3.2 4,399,737 - Pf6N2E2_3775 0.19 -1.0 4,399,737 - Pf6N2E2_3775 0.19 +0.2 4,399,737 - Pf6N2E2_3775 0.19 +0.6 4,399,737 - Pf6N2E2_3775 0.19 +0.2 4,399,740 - Pf6N2E2_3775 0.19 -0.6 4,399,893 - Pf6N2E2_3775 0.44 -2.0 4,400,050 - Pf6N2E2_3775 0.69 -1.2 4,400,099 + Pf6N2E2_3775 0.77 -1.3 4,400,100 - Pf6N2E2_3775 0.77 +0.6 4,400,100 - Pf6N2E2_3775 0.77 +0.2 4,400,219 + +0.5 4,400,238 - -0.6 4,400,417 - -1.4 4,400,417 - -1.7 4,400,417 - -0.3 4,401,195 - +0.0 4,401,223 - +1.0 4,401,223 - +0.3 4,401,303 - +0.3 4,401,372 - Pf6N2E2_3777 0.18 +0.2 4,401,372 - Pf6N2E2_3777 0.18 -1.6 4,401,372 - Pf6N2E2_3777 0.18 -1.6 4,401,445 - Pf6N2E2_3777 0.36 +0.6 4,401,445 - Pf6N2E2_3777 0.36 -0.8 4,401,568 - Pf6N2E2_3777 0.65 -1.1 4,401,568 - Pf6N2E2_3777 0.65 -0.7 4,401,650 - Pf6N2E2_3777 0.84 +1.1 4,401,650 - Pf6N2E2_3777 0.84 +0.4 4,401,650 - Pf6N2E2_3777 0.84 -1.1 4,401,780 + -0.5 4,401,780 + +0.2 4,401,781 - +0.6 4,401,789 + +0.1 4,401,789 + -0.4 4,401,789 + -0.3 4,401,790 - +0.6 4,401,790 - +0.3 4,401,790 - +0.4 4,401,790 - -0.7 4,401,790 - -2.2 4,401,790 - +0.7 4,401,794 - +0.6 4,401,807 + +0.9 4,401,807 + +0.3 4,401,808 - +1.0 4,401,901 - +0.6 4,401,908 - -0.3 4,401,960 - -0.4 4,401,960 - -0.6 4,401,960 - -0.1 4,401,960 - -1.2 4,401,960 - -0.1 4,401,960 - -0.3 4,401,960 - -0.4 4,402,160 + Pf6N2E2_3778 0.66 -0.9 4,402,160 + Pf6N2E2_3778 0.66 -0.2 4,402,161 - Pf6N2E2_3778 0.66 +0.1 4,402,165 - Pf6N2E2_3778 0.67 +0.0 4,402,243 + +0.1 4,402,245 + +0.2 4,402,245 + +0.1 4,402,245 + +0.9 4,402,246 - -1.7 4,402,246 - +0.1 4,402,246 - -2.8
Or see this region's nucleotide sequence