Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3534 and Pf6N2E2_3535 are separated by 390 nucleotides Pf6N2E2_3535 and Pf6N2E2_3536 overlap by 8 nucleotides
Pf6N2E2_3534 - Cobalamin biosynthesis protein CobG, at 4,124,063 to 4,125,331
_3534
Pf6N2E2_3535 - Cobalt-precorrin-6y C5-methyltransferase (EC 2.1.1.-) / Cobalt-precorrin-6y C15-methyltransferase [decarboxylating] (EC 2.1.1.-), at 4,125,722 to 4,126,933
_3535
Pf6N2E2_3536 - Cobalt-precorrin-6 synthase, anaerobic, at 4,126,926 to 4,128,023
_3536
Position (kb) 4125
4126
4127 Strain fitness (log2 ratio) -2
-1
0
1 at 4125.035 kb on - strand, within Pf6N2E2_3534 at 4125.353 kb on + strand at 4125.353 kb on + strand at 4125.354 kb on - strand at 4125.354 kb on - strand at 4125.395 kb on - strand at 4125.430 kb on + strand at 4125.430 kb on + strand at 4125.431 kb on - strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.506 kb on + strand at 4125.507 kb on - strand at 4125.507 kb on - strand at 4125.638 kb on + strand at 4125.639 kb on - strand at 4125.639 kb on - strand at 4125.639 kb on - strand at 4125.682 kb on - strand at 4125.741 kb on + strand at 4125.962 kb on + strand, within Pf6N2E2_3535 at 4125.962 kb on + strand, within Pf6N2E2_3535 at 4125.962 kb on + strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4125.963 kb on - strand, within Pf6N2E2_3535 at 4126.128 kb on + strand, within Pf6N2E2_3535 at 4126.128 kb on + strand, within Pf6N2E2_3535 at 4126.128 kb on + strand, within Pf6N2E2_3535 at 4126.356 kb on + strand, within Pf6N2E2_3535 at 4126.534 kb on + strand, within Pf6N2E2_3535 at 4126.534 kb on + strand, within Pf6N2E2_3535 at 4126.534 kb on + strand, within Pf6N2E2_3535 at 4126.535 kb on - strand, within Pf6N2E2_3535 at 4126.535 kb on - strand, within Pf6N2E2_3535 at 4126.700 kb on - strand, within Pf6N2E2_3535 at 4127.060 kb on - strand, within Pf6N2E2_3536 at 4127.060 kb on - strand, within Pf6N2E2_3536 at 4127.218 kb on + strand, within Pf6N2E2_3536 at 4127.218 kb on + strand, within Pf6N2E2_3536 at 4127.218 kb on + strand, within Pf6N2E2_3536 at 4127.219 kb on - strand, within Pf6N2E2_3536 at 4127.356 kb on - strand, within Pf6N2E2_3536 at 4127.499 kb on + strand, within Pf6N2E2_3536 at 4127.499 kb on + strand, within Pf6N2E2_3536 at 4127.499 kb on + strand, within Pf6N2E2_3536 at 4127.500 kb on - strand, within Pf6N2E2_3536 at 4127.500 kb on - strand, within Pf6N2E2_3536 at 4127.500 kb on - strand, within Pf6N2E2_3536 at 4127.500 kb on - strand, within Pf6N2E2_3536 at 4127.604 kb on + strand, within Pf6N2E2_3536 at 4127.605 kb on - strand, within Pf6N2E2_3536 at 4127.605 kb on - strand, within Pf6N2E2_3536
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 4,125,035 - Pf6N2E2_3534 0.77 -0.9 4,125,353 + -0.8 4,125,353 + +1.3 4,125,354 - -0.4 4,125,354 - -1.7 4,125,395 - +0.7 4,125,430 + -2.4 4,125,430 + +1.3 4,125,431 - -0.4 4,125,506 + -0.5 4,125,506 + +0.1 4,125,506 + -1.1 4,125,506 + +0.2 4,125,506 + -1.1 4,125,506 + +0.3 4,125,507 - +1.6 4,125,507 - +0.5 4,125,638 + -0.1 4,125,639 - +1.0 4,125,639 - +0.7 4,125,639 - +0.8 4,125,682 - +0.6 4,125,741 + +0.7 4,125,962 + Pf6N2E2_3535 0.20 -2.6 4,125,962 + Pf6N2E2_3535 0.20 -0.8 4,125,962 + Pf6N2E2_3535 0.20 -0.9 4,125,963 - Pf6N2E2_3535 0.20 +1.4 4,125,963 - Pf6N2E2_3535 0.20 -0.9 4,125,963 - Pf6N2E2_3535 0.20 -0.5 4,125,963 - Pf6N2E2_3535 0.20 -1.6 4,125,963 - Pf6N2E2_3535 0.20 -1.9 4,126,128 + Pf6N2E2_3535 0.33 -0.6 4,126,128 + Pf6N2E2_3535 0.33 +0.6 4,126,128 + Pf6N2E2_3535 0.33 -2.6 4,126,356 + Pf6N2E2_3535 0.52 +0.5 4,126,534 + Pf6N2E2_3535 0.67 -1.3 4,126,534 + Pf6N2E2_3535 0.67 -1.0 4,126,534 + Pf6N2E2_3535 0.67 -2.1 4,126,535 - Pf6N2E2_3535 0.67 -1.0 4,126,535 - Pf6N2E2_3535 0.67 -1.3 4,126,700 - Pf6N2E2_3535 0.81 -0.2 4,127,060 - Pf6N2E2_3536 0.12 -0.1 4,127,060 - Pf6N2E2_3536 0.12 -1.2 4,127,218 + Pf6N2E2_3536 0.27 -1.9 4,127,218 + Pf6N2E2_3536 0.27 -1.1 4,127,218 + Pf6N2E2_3536 0.27 -0.4 4,127,219 - Pf6N2E2_3536 0.27 -0.4 4,127,356 - Pf6N2E2_3536 0.39 +0.2 4,127,499 + Pf6N2E2_3536 0.52 +0.1 4,127,499 + Pf6N2E2_3536 0.52 +1.0 4,127,499 + Pf6N2E2_3536 0.52 +1.6 4,127,500 - Pf6N2E2_3536 0.52 -0.5 4,127,500 - Pf6N2E2_3536 0.52 +0.7 4,127,500 - Pf6N2E2_3536 0.52 -1.5 4,127,500 - Pf6N2E2_3536 0.52 +0.4 4,127,604 + Pf6N2E2_3536 0.62 -0.7 4,127,605 - Pf6N2E2_3536 0.62 -2.6 4,127,605 - Pf6N2E2_3536 0.62 -1.0
Or see this region's nucleotide sequence