Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3515 and Pf6N2E2_3516 are separated by 13 nucleotides Pf6N2E2_3516 and Pf6N2E2_3517 are separated by 147 nucleotides Pf6N2E2_3517 and Pf6N2E2_3518 are separated by 38 nucleotides Pf6N2E2_3518 and Pf6N2E2_3519 are separated by 83 nucleotides
Pf6N2E2_3515 - Probable transmembrane protein, at 4,099,860 to 4,100,942
_3515
Pf6N2E2_3516 - Probable transmembrane protein, at 4,100,956 to 4,101,090
_3516
Pf6N2E2_3517 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 4,101,238 to 4,102,245
_3517
Pf6N2E2_3518 - DNA-binding protein Fis, at 4,102,284 to 4,102,562
_3518
Pf6N2E2_3519 - IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3), at 4,102,646 to 4,104,253
_3519
Position (kb)
4101
4102
4103 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.256 kb on + strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.603 kb on - strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.635 kb on + strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.636 kb on - strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.723 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.821 kb on + strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.822 kb on - strand, within Pf6N2E2_3515 at 4100.923 kb on + strand at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.022 kb on + strand, within Pf6N2E2_3516 at 4101.023 kb on - strand, within Pf6N2E2_3516 at 4101.129 kb on + strand at 4101.129 kb on + strand at 4101.130 kb on - strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.237 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.262 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.264 kb on + strand at 4101.775 kb on + strand, within Pf6N2E2_3517 at 4101.775 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.017 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.043 kb on + strand, within Pf6N2E2_3517 at 4102.073 kb on + strand, within Pf6N2E2_3517 at 4102.161 kb on + strand at 4102.207 kb on + strand at 4102.207 kb on + strand at 4102.207 kb on + strand at 4102.264 kb on + strand at 4102.560 kb on + strand at 4102.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 4,100,256 + Pf6N2E2_3515 0.37 +0.7 4,100,256 + Pf6N2E2_3515 0.37 -0.9 4,100,603 - Pf6N2E2_3515 0.69 -1.0 4,100,603 - Pf6N2E2_3515 0.69 -0.2 4,100,635 + Pf6N2E2_3515 0.72 +0.1 4,100,635 + Pf6N2E2_3515 0.72 -0.4 4,100,635 + Pf6N2E2_3515 0.72 -1.7 4,100,635 + Pf6N2E2_3515 0.72 +0.5 4,100,636 - Pf6N2E2_3515 0.72 -1.6 4,100,636 - Pf6N2E2_3515 0.72 -0.7 4,100,636 - Pf6N2E2_3515 0.72 -0.3 4,100,636 - Pf6N2E2_3515 0.72 -1.6 4,100,723 + Pf6N2E2_3515 0.80 -0.6 4,100,723 + Pf6N2E2_3515 0.80 -1.5 4,100,821 + Pf6N2E2_3515 0.89 -0.8 4,100,821 + Pf6N2E2_3515 0.89 +0.3 4,100,821 + Pf6N2E2_3515 0.89 -1.9 4,100,821 + Pf6N2E2_3515 0.89 -0.1 4,100,822 - Pf6N2E2_3515 0.89 -1.5 4,100,822 - Pf6N2E2_3515 0.89 +2.2 4,100,822 - Pf6N2E2_3515 0.89 +0.4 4,100,822 - Pf6N2E2_3515 0.89 -1.8 4,100,822 - Pf6N2E2_3515 0.89 -2.6 4,100,923 + -1.5 4,101,022 + Pf6N2E2_3516 0.49 -1.5 4,101,022 + Pf6N2E2_3516 0.49 -1.9 4,101,023 - Pf6N2E2_3516 0.50 +0.3 4,101,129 + +0.1 4,101,129 + +0.5 4,101,130 - +1.5 4,101,237 + -0.1 4,101,237 + -2.5 4,101,237 + -0.4 4,101,237 + -0.7 4,101,237 + +0.9 4,101,262 + +1.5 4,101,262 + -0.6 4,101,262 + +0.7 4,101,262 + +1.1 4,101,264 + +1.3 4,101,264 + +0.9 4,101,264 + +0.2 4,101,264 + -0.4 4,101,264 + -2.2 4,101,264 + +0.8 4,101,264 + +0.7 4,101,264 + +0.5 4,101,264 + +0.6 4,101,264 + -0.0 4,101,264 + -0.6 4,101,264 + +1.7 4,101,264 + +0.7 4,101,264 + -1.1 4,101,264 + +0.3 4,101,264 + +0.5 4,101,264 + +0.4 4,101,264 + +0.3 4,101,775 + Pf6N2E2_3517 0.53 -0.7 4,101,775 + Pf6N2E2_3517 0.53 -0.3 4,102,017 + Pf6N2E2_3517 0.77 -1.1 4,102,017 + Pf6N2E2_3517 0.77 -0.8 4,102,017 + Pf6N2E2_3517 0.77 +0.4 4,102,017 + Pf6N2E2_3517 0.77 -1.2 4,102,017 + Pf6N2E2_3517 0.77 -2.0 4,102,017 + Pf6N2E2_3517 0.77 +0.5 4,102,043 + Pf6N2E2_3517 0.80 -1.6 4,102,043 + Pf6N2E2_3517 0.80 -1.4 4,102,043 + Pf6N2E2_3517 0.80 -1.9 4,102,043 + Pf6N2E2_3517 0.80 -0.2 4,102,073 + Pf6N2E2_3517 0.83 -0.8 4,102,161 + -0.8 4,102,207 + +0.2 4,102,207 + -0.4 4,102,207 + -0.7 4,102,264 + -0.8 4,102,560 + +0.8 4,102,560 + -0.2
Or see this region's nucleotide sequence