Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides Pf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides Pf6N2E2_351 and Pf6N2E2_352 are separated by 203 nucleotides
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Position (kb) 416
417
418 Strain fitness (log2 ratio) -2
-1
0
1
2 at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand at 416.131 kb on + strand, within Pf6N2E2_350 at 416.192 kb on - strand, within Pf6N2E2_350 at 416.231 kb on - strand at 416.231 kb on - strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.297 kb on - strand at 416.323 kb on + strand at 416.449 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.585 kb on - strand, within Pf6N2E2_351 at 417.212 kb on - strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.330 kb on + strand, within Pf6N2E2_351 at 417.531 kb on + strand, within Pf6N2E2_351 at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.701 kb on + strand at 417.701 kb on + strand at 417.712 kb on + strand at 417.712 kb on + strand at 417.713 kb on - strand at 417.713 kb on - strand at 417.759 kb on + strand at 417.760 kb on - strand at 417.760 kb on - strand at 417.781 kb on + strand at 417.818 kb on + strand at 417.819 kb on - strand at 417.819 kb on - strand at 418.075 kb on - strand, within Pf6N2E2_352 at 418.075 kb on - strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.317 kb on + strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.335 kb on - strand, within Pf6N2E2_352 at 418.496 kb on + strand, within Pf6N2E2_352 at 418.497 kb on - strand, within Pf6N2E2_352 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 415,347 + Pf6N2E2_349 0.42 +0.2 415,515 + Pf6N2E2_349 0.65 +0.6 415,516 - Pf6N2E2_349 0.65 +0.2 415,542 - Pf6N2E2_349 0.68 -2.0 415,660 + Pf6N2E2_349 0.84 +0.4 415,660 + Pf6N2E2_349 0.84 -0.4 415,772 + +0.7 415,773 - +0.4 415,806 + -1.3 415,807 - +2.2 415,807 - +0.7 415,807 - -2.0 415,807 - +0.6 415,865 + -1.1 416,131 + Pf6N2E2_350 0.74 -0.8 416,192 - Pf6N2E2_350 0.90 -1.3 416,231 - +1.1 416,231 - +0.1 416,272 + -0.2 416,272 + -0.7 416,272 + -0.0 416,297 - -1.1 416,323 + +1.0 416,449 + Pf6N2E2_351 0.15 -0.6 416,584 + Pf6N2E2_351 0.24 +1.7 416,584 + Pf6N2E2_351 0.24 +0.7 416,585 - Pf6N2E2_351 0.24 +0.6 417,212 - Pf6N2E2_351 0.67 -0.1 417,301 + Pf6N2E2_351 0.73 +0.4 417,301 + Pf6N2E2_351 0.73 +1.7 417,301 + Pf6N2E2_351 0.73 -0.8 417,302 - Pf6N2E2_351 0.73 -2.1 417,302 - Pf6N2E2_351 0.73 -0.6 417,330 + Pf6N2E2_351 0.75 +0.6 417,531 + Pf6N2E2_351 0.89 -0.3 417,640 + +0.0 417,640 + -1.1 417,640 + -1.1 417,640 + -0.8 417,641 - -1.4 417,641 - -1.0 417,641 - +0.3 417,641 - -0.5 417,641 - +0.7 417,701 + +0.4 417,701 + -0.4 417,712 + +0.3 417,712 + +0.3 417,713 - +1.3 417,713 - -2.1 417,759 + +0.1 417,760 - -1.3 417,760 - -0.1 417,781 + -1.2 417,818 + -0.3 417,819 - +0.7 417,819 - -0.2 418,075 - Pf6N2E2_352 0.16 +1.0 418,075 - Pf6N2E2_352 0.16 +0.7 418,131 + Pf6N2E2_352 0.20 +0.3 418,131 + Pf6N2E2_352 0.20 -0.3 418,132 - Pf6N2E2_352 0.20 -0.4 418,132 - Pf6N2E2_352 0.20 +0.5 418,132 - Pf6N2E2_352 0.20 -1.7 418,317 + Pf6N2E2_352 0.36 +1.1 418,318 - Pf6N2E2_352 0.36 +1.1 418,318 - Pf6N2E2_352 0.36 -2.1 418,335 - Pf6N2E2_352 0.38 +0.7 418,496 + Pf6N2E2_352 0.51 +2.5 418,497 - Pf6N2E2_352 0.51 +0.5 418,653 + Pf6N2E2_352 0.65 -0.4 418,654 - Pf6N2E2_352 0.65 -0.5
Or see this region's nucleotide sequence