Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3297 and Pf6N2E2_3298 are separated by 85 nucleotides Pf6N2E2_3298 and Pf6N2E2_3299 are separated by 193 nucleotides
Pf6N2E2_3297 - D-galactarate permease, at 3,804,118 to 3,805,482
_3297
Pf6N2E2_3298 - 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26), at 3,805,568 to 3,807,013
_3298
Pf6N2E2_3299 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41), at 3,807,207 to 3,808,118
_3299
Position (kb)
3805
3806
3807
3808 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3804.674 kb on + strand, within Pf6N2E2_3297 at 3804.674 kb on + strand, within Pf6N2E2_3297 at 3804.675 kb on - strand, within Pf6N2E2_3297 at 3804.701 kb on + strand, within Pf6N2E2_3297 at 3804.701 kb on + strand, within Pf6N2E2_3297 at 3804.702 kb on - strand, within Pf6N2E2_3297 at 3804.702 kb on - strand, within Pf6N2E2_3297 at 3804.723 kb on - strand, within Pf6N2E2_3297 at 3804.734 kb on + strand, within Pf6N2E2_3297 at 3804.734 kb on + strand, within Pf6N2E2_3297 at 3804.735 kb on - strand, within Pf6N2E2_3297 at 3804.776 kb on + strand, within Pf6N2E2_3297 at 3804.776 kb on + strand, within Pf6N2E2_3297 at 3804.777 kb on - strand, within Pf6N2E2_3297 at 3804.777 kb on - strand, within Pf6N2E2_3297 at 3804.902 kb on + strand, within Pf6N2E2_3297 at 3804.902 kb on + strand, within Pf6N2E2_3297 at 3804.902 kb on + strand, within Pf6N2E2_3297 at 3804.903 kb on - strand, within Pf6N2E2_3297 at 3804.903 kb on - strand, within Pf6N2E2_3297 at 3804.903 kb on - strand, within Pf6N2E2_3297 at 3805.029 kb on - strand, within Pf6N2E2_3297 at 3805.029 kb on - strand, within Pf6N2E2_3297 at 3805.196 kb on + strand, within Pf6N2E2_3297 at 3805.196 kb on + strand, within Pf6N2E2_3297 at 3805.196 kb on + strand, within Pf6N2E2_3297 at 3805.197 kb on - strand, within Pf6N2E2_3297 at 3805.197 kb on - strand, within Pf6N2E2_3297 at 3805.197 kb on - strand, within Pf6N2E2_3297 at 3805.244 kb on + strand, within Pf6N2E2_3297 at 3805.305 kb on - strand, within Pf6N2E2_3297 at 3805.329 kb on + strand, within Pf6N2E2_3297 at 3805.329 kb on + strand, within Pf6N2E2_3297 at 3805.329 kb on + strand, within Pf6N2E2_3297 at 3805.329 kb on + strand, within Pf6N2E2_3297 at 3805.329 kb on + strand, within Pf6N2E2_3297 at 3805.329 kb on + strand, within Pf6N2E2_3297 at 3805.330 kb on - strand, within Pf6N2E2_3297 at 3805.330 kb on - strand, within Pf6N2E2_3297 at 3805.330 kb on - strand, within Pf6N2E2_3297 at 3805.330 kb on - strand, within Pf6N2E2_3297 at 3805.330 kb on - strand, within Pf6N2E2_3297 at 3805.330 kb on - strand, within Pf6N2E2_3297 at 3805.331 kb on + strand, within Pf6N2E2_3297 at 3805.331 kb on + strand, within Pf6N2E2_3297 at 3805.331 kb on + strand, within Pf6N2E2_3297 at 3805.331 kb on + strand, within Pf6N2E2_3297 at 3805.331 kb on + strand, within Pf6N2E2_3297 at 3805.332 kb on - strand, within Pf6N2E2_3297 at 3805.409 kb on + strand at 3805.409 kb on + strand at 3805.409 kb on + strand at 3805.409 kb on + strand at 3805.409 kb on + strand at 3805.409 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.484 kb on + strand at 3805.485 kb on - strand at 3805.490 kb on + strand at 3805.490 kb on + strand at 3805.490 kb on + strand at 3805.491 kb on - strand at 3805.491 kb on - strand at 3805.499 kb on + strand at 3805.500 kb on - strand at 3805.504 kb on - strand at 3805.504 kb on - strand at 3805.510 kb on - strand at 3805.569 kb on + strand at 3805.569 kb on + strand at 3805.570 kb on - strand at 3805.570 kb on - strand at 3805.570 kb on - strand at 3805.570 kb on - strand at 3805.570 kb on - strand at 3805.570 kb on - strand at 3805.581 kb on + strand at 3805.581 kb on + strand at 3805.582 kb on - strand at 3805.582 kb on - strand at 3805.582 kb on - strand at 3805.591 kb on + strand at 3805.602 kb on + strand at 3805.602 kb on + strand at 3805.603 kb on - strand at 3805.603 kb on - strand at 3805.603 kb on - strand at 3805.641 kb on + strand at 3805.641 kb on + strand at 3805.642 kb on - strand at 3805.678 kb on - strand at 3805.678 kb on - strand at 3805.750 kb on - strand, within Pf6N2E2_3298 at 3805.765 kb on + strand, within Pf6N2E2_3298 at 3805.824 kb on + strand, within Pf6N2E2_3298 at 3805.825 kb on - strand, within Pf6N2E2_3298 at 3805.927 kb on - strand, within Pf6N2E2_3298 at 3805.927 kb on - strand, within Pf6N2E2_3298 at 3806.001 kb on + strand, within Pf6N2E2_3298 at 3806.001 kb on + strand, within Pf6N2E2_3298 at 3806.001 kb on + strand, within Pf6N2E2_3298 at 3806.002 kb on - strand, within Pf6N2E2_3298 at 3806.640 kb on + strand, within Pf6N2E2_3298 at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.985 kb on + strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3806.986 kb on - strand at 3807.026 kb on + strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.042 kb on + strand at 3807.043 kb on - strand at 3807.043 kb on - strand at 3807.043 kb on - strand at 3807.043 kb on - strand at 3807.105 kb on + strand at 3807.166 kb on - strand at 3807.208 kb on + strand at 3807.209 kb on - strand at 3807.250 kb on + strand at 3807.250 kb on + strand at 3807.250 kb on + strand at 3807.250 kb on + strand at 3807.251 kb on - strand at 3807.251 kb on - strand at 3807.373 kb on + strand, within Pf6N2E2_3299 at 3807.374 kb on - strand, within Pf6N2E2_3299 at 3807.439 kb on + strand, within Pf6N2E2_3299 at 3807.487 kb on + strand, within Pf6N2E2_3299 at 3807.488 kb on - strand, within Pf6N2E2_3299 at 3807.512 kb on - strand, within Pf6N2E2_3299 at 3807.553 kb on + strand, within Pf6N2E2_3299 at 3807.554 kb on - strand, within Pf6N2E2_3299 at 3807.697 kb on + strand, within Pf6N2E2_3299 at 3807.698 kb on - strand, within Pf6N2E2_3299 at 3807.779 kb on - strand, within Pf6N2E2_3299 at 3807.779 kb on - strand, within Pf6N2E2_3299 at 3807.832 kb on + strand, within Pf6N2E2_3299 at 3807.833 kb on - strand, within Pf6N2E2_3299 at 3807.833 kb on - strand, within Pf6N2E2_3299 at 3807.925 kb on + strand, within Pf6N2E2_3299 at 3807.992 kb on - strand, within Pf6N2E2_3299
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 3,804,674 + Pf6N2E2_3297 0.41 +0.0 3,804,674 + Pf6N2E2_3297 0.41 -0.2 3,804,675 - Pf6N2E2_3297 0.41 -1.4 3,804,701 + Pf6N2E2_3297 0.43 +1.3 3,804,701 + Pf6N2E2_3297 0.43 +0.6 3,804,702 - Pf6N2E2_3297 0.43 +1.2 3,804,702 - Pf6N2E2_3297 0.43 -0.6 3,804,723 - Pf6N2E2_3297 0.44 -0.8 3,804,734 + Pf6N2E2_3297 0.45 -1.3 3,804,734 + Pf6N2E2_3297 0.45 -1.2 3,804,735 - Pf6N2E2_3297 0.45 -0.0 3,804,776 + Pf6N2E2_3297 0.48 -0.4 3,804,776 + Pf6N2E2_3297 0.48 +0.9 3,804,777 - Pf6N2E2_3297 0.48 -0.5 3,804,777 - Pf6N2E2_3297 0.48 -0.7 3,804,902 + Pf6N2E2_3297 0.57 +0.4 3,804,902 + Pf6N2E2_3297 0.57 -0.6 3,804,902 + Pf6N2E2_3297 0.57 -1.1 3,804,903 - Pf6N2E2_3297 0.58 +1.1 3,804,903 - Pf6N2E2_3297 0.58 +1.4 3,804,903 - Pf6N2E2_3297 0.58 -1.2 3,805,029 - Pf6N2E2_3297 0.67 +0.1 3,805,029 - Pf6N2E2_3297 0.67 +0.6 3,805,196 + Pf6N2E2_3297 0.79 -0.4 3,805,196 + Pf6N2E2_3297 0.79 -0.0 3,805,196 + Pf6N2E2_3297 0.79 +0.6 3,805,197 - Pf6N2E2_3297 0.79 -0.2 3,805,197 - Pf6N2E2_3297 0.79 -1.8 3,805,197 - Pf6N2E2_3297 0.79 +1.4 3,805,244 + Pf6N2E2_3297 0.82 +0.5 3,805,305 - Pf6N2E2_3297 0.87 -0.0 3,805,329 + Pf6N2E2_3297 0.89 -0.8 3,805,329 + Pf6N2E2_3297 0.89 +1.4 3,805,329 + Pf6N2E2_3297 0.89 +0.6 3,805,329 + Pf6N2E2_3297 0.89 +0.3 3,805,329 + Pf6N2E2_3297 0.89 +1.1 3,805,329 + Pf6N2E2_3297 0.89 -0.0 3,805,330 - Pf6N2E2_3297 0.89 -1.4 3,805,330 - Pf6N2E2_3297 0.89 -1.4 3,805,330 - Pf6N2E2_3297 0.89 -2.0 3,805,330 - Pf6N2E2_3297 0.89 +0.7 3,805,330 - Pf6N2E2_3297 0.89 +1.0 3,805,330 - Pf6N2E2_3297 0.89 -0.3 3,805,331 + Pf6N2E2_3297 0.89 -1.2 3,805,331 + Pf6N2E2_3297 0.89 -0.4 3,805,331 + Pf6N2E2_3297 0.89 -1.1 3,805,331 + Pf6N2E2_3297 0.89 +0.7 3,805,331 + Pf6N2E2_3297 0.89 +0.8 3,805,332 - Pf6N2E2_3297 0.89 -0.9 3,805,409 + +0.2 3,805,409 + -1.1 3,805,409 + -0.7 3,805,409 + +0.0 3,805,409 + -0.9 3,805,409 + +0.9 3,805,484 + -0.8 3,805,484 + +0.1 3,805,484 + -0.8 3,805,484 + -0.6 3,805,484 + +1.4 3,805,484 + +0.5 3,805,484 + +0.4 3,805,484 + +0.4 3,805,485 - -1.1 3,805,490 + +0.4 3,805,490 + -1.1 3,805,490 + +0.0 3,805,491 - +0.9 3,805,491 - +1.0 3,805,499 + -0.6 3,805,500 - +2.2 3,805,504 - +1.0 3,805,504 - -0.6 3,805,510 - +1.3 3,805,569 + -0.0 3,805,569 + +0.2 3,805,570 - -0.2 3,805,570 - -0.6 3,805,570 - -1.6 3,805,570 - -1.0 3,805,570 - -0.7 3,805,570 - +0.4 3,805,581 + -1.3 3,805,581 + -0.3 3,805,582 - +0.8 3,805,582 - +0.2 3,805,582 - +0.5 3,805,591 + +0.7 3,805,602 + +0.6 3,805,602 + +0.6 3,805,603 - +0.6 3,805,603 - +0.3 3,805,603 - +0.0 3,805,641 + +0.7 3,805,641 + -0.7 3,805,642 - -0.1 3,805,678 - -0.1 3,805,678 - +0.2 3,805,750 - Pf6N2E2_3298 0.13 +0.9 3,805,765 + Pf6N2E2_3298 0.14 -2.0 3,805,824 + Pf6N2E2_3298 0.18 -1.7 3,805,825 - Pf6N2E2_3298 0.18 +0.6 3,805,927 - Pf6N2E2_3298 0.25 +0.9 3,805,927 - Pf6N2E2_3298 0.25 +1.1 3,806,001 + Pf6N2E2_3298 0.30 +0.6 3,806,001 + Pf6N2E2_3298 0.30 +1.8 3,806,001 + Pf6N2E2_3298 0.30 -0.5 3,806,002 - Pf6N2E2_3298 0.30 +0.4 3,806,640 + Pf6N2E2_3298 0.74 +0.4 3,806,985 + -0.8 3,806,985 + -1.1 3,806,985 + -1.4 3,806,985 + +1.7 3,806,985 + +0.3 3,806,985 + -0.1 3,806,985 + -0.8 3,806,985 + -0.5 3,806,985 + +0.1 3,806,985 + -1.0 3,806,985 + +0.1 3,806,985 + -0.4 3,806,985 + -0.4 3,806,986 - -0.3 3,806,986 - +0.3 3,806,986 - -0.5 3,806,986 - -0.9 3,806,986 - +0.0 3,806,986 - -0.4 3,806,986 - -0.4 3,806,986 - +0.0 3,806,986 - +0.2 3,806,986 - +1.0 3,806,986 - +0.8 3,806,986 - +0.6 3,807,026 + -1.4 3,807,027 - +0.2 3,807,027 - -0.4 3,807,042 + +0.4 3,807,043 - +1.6 3,807,043 - +0.1 3,807,043 - +0.5 3,807,043 - +0.6 3,807,105 + +0.2 3,807,166 - +1.0 3,807,208 + -1.5 3,807,209 - -1.1 3,807,250 + -1.8 3,807,250 + -0.7 3,807,250 + +0.4 3,807,250 + +0.3 3,807,251 - -0.3 3,807,251 - +1.6 3,807,373 + Pf6N2E2_3299 0.18 -0.4 3,807,374 - Pf6N2E2_3299 0.18 +1.7 3,807,439 + Pf6N2E2_3299 0.25 -0.0 3,807,487 + Pf6N2E2_3299 0.31 +1.3 3,807,488 - Pf6N2E2_3299 0.31 -0.3 3,807,512 - Pf6N2E2_3299 0.33 +0.1 3,807,553 + Pf6N2E2_3299 0.38 -0.3 3,807,554 - Pf6N2E2_3299 0.38 +0.9 3,807,697 + Pf6N2E2_3299 0.54 +3.2 3,807,698 - Pf6N2E2_3299 0.54 -0.3 3,807,779 - Pf6N2E2_3299 0.63 -2.2 3,807,779 - Pf6N2E2_3299 0.63 -1.5 3,807,832 + Pf6N2E2_3299 0.69 +0.2 3,807,833 - Pf6N2E2_3299 0.69 +1.8 3,807,833 - Pf6N2E2_3299 0.69 -0.5 3,807,925 + Pf6N2E2_3299 0.79 -0.2 3,807,992 - Pf6N2E2_3299 0.86 +0.8
Or see this region's nucleotide sequence