Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_279 and Pf6N2E2_280 overlap by 24 nucleotides Pf6N2E2_280 and Pf6N2E2_281 are separated by 323 nucleotides
Pf6N2E2_279 - Isocitrate lyase (EC 4.1.3.1), at 345,874 to 347,199
_279
Pf6N2E2_280 - hypothetical protein, at 347,176 to 347,331
_280
Pf6N2E2_281 - Type II and III secretion system family protein, at 347,655 to 348,416
_281
Position (kb) 347
348 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 346.229 kb on + strand, within Pf6N2E2_279 at 346.229 kb on + strand, within Pf6N2E2_279 at 346.229 kb on + strand, within Pf6N2E2_279 at 346.230 kb on - strand, within Pf6N2E2_279 at 346.388 kb on + strand, within Pf6N2E2_279 at 346.388 kb on + strand, within Pf6N2E2_279 at 346.388 kb on + strand, within Pf6N2E2_279 at 346.388 kb on + strand, within Pf6N2E2_279 at 346.388 kb on + strand, within Pf6N2E2_279 at 346.388 kb on + strand, within Pf6N2E2_279 at 346.389 kb on - strand, within Pf6N2E2_279 at 346.389 kb on - strand, within Pf6N2E2_279 at 346.389 kb on - strand, within Pf6N2E2_279 at 346.480 kb on - strand, within Pf6N2E2_279 at 346.670 kb on + strand, within Pf6N2E2_279 at 346.728 kb on - strand, within Pf6N2E2_279 at 346.736 kb on + strand, within Pf6N2E2_279 at 346.737 kb on - strand, within Pf6N2E2_279 at 346.737 kb on - strand, within Pf6N2E2_279 at 346.737 kb on - strand, within Pf6N2E2_279 at 346.841 kb on + strand, within Pf6N2E2_279 at 346.910 kb on + strand, within Pf6N2E2_279 at 346.910 kb on + strand, within Pf6N2E2_279 at 346.910 kb on + strand, within Pf6N2E2_279 at 346.911 kb on - strand, within Pf6N2E2_279 at 346.911 kb on - strand, within Pf6N2E2_279 at 346.911 kb on - strand, within Pf6N2E2_279 at 347.205 kb on + strand, within Pf6N2E2_280 at 347.205 kb on + strand, within Pf6N2E2_280 at 347.219 kb on + strand, within Pf6N2E2_280 at 347.220 kb on - strand, within Pf6N2E2_280 at 347.266 kb on - strand, within Pf6N2E2_280 at 347.329 kb on + strand at 347.330 kb on - strand at 347.371 kb on + strand at 347.399 kb on + strand at 347.399 kb on + strand at 347.399 kb on + strand at 347.400 kb on - strand at 347.400 kb on - strand at 347.400 kb on - strand at 347.400 kb on - strand at 347.403 kb on + strand at 347.403 kb on + strand at 347.403 kb on + strand at 347.403 kb on + strand at 347.404 kb on - strand at 347.611 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.713 kb on + strand at 347.714 kb on - strand at 347.826 kb on + strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand, within Pf6N2E2_281 at 347.827 kb on - strand at 347.911 kb on + strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.912 kb on - strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.962 kb on + strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 347.963 kb on - strand, within Pf6N2E2_281 at 348.165 kb on - strand, within Pf6N2E2_281
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 346,229 + Pf6N2E2_279 0.27 +1.6 346,229 + Pf6N2E2_279 0.27 +0.9 346,229 + Pf6N2E2_279 0.27 +0.4 346,230 - Pf6N2E2_279 0.27 -1.4 346,388 + Pf6N2E2_279 0.39 -1.2 346,388 + Pf6N2E2_279 0.39 -1.3 346,388 + Pf6N2E2_279 0.39 +0.1 346,388 + Pf6N2E2_279 0.39 -2.7 346,388 + Pf6N2E2_279 0.39 -0.4 346,388 + Pf6N2E2_279 0.39 +1.8 346,389 - Pf6N2E2_279 0.39 -1.0 346,389 - Pf6N2E2_279 0.39 -1.7 346,389 - Pf6N2E2_279 0.39 +0.5 346,480 - Pf6N2E2_279 0.46 +1.2 346,670 + Pf6N2E2_279 0.60 +0.2 346,728 - Pf6N2E2_279 0.64 +0.5 346,736 + Pf6N2E2_279 0.65 -1.9 346,737 - Pf6N2E2_279 0.65 -0.2 346,737 - Pf6N2E2_279 0.65 -0.0 346,737 - Pf6N2E2_279 0.65 -0.7 346,841 + Pf6N2E2_279 0.73 -1.9 346,910 + Pf6N2E2_279 0.78 -0.4 346,910 + Pf6N2E2_279 0.78 -0.0 346,910 + Pf6N2E2_279 0.78 -1.0 346,911 - Pf6N2E2_279 0.78 -0.7 346,911 - Pf6N2E2_279 0.78 -0.9 346,911 - Pf6N2E2_279 0.78 -0.1 347,205 + Pf6N2E2_280 0.19 -1.2 347,205 + Pf6N2E2_280 0.19 -1.2 347,219 + Pf6N2E2_280 0.28 -0.7 347,220 - Pf6N2E2_280 0.28 -0.3 347,266 - Pf6N2E2_280 0.58 -1.0 347,329 + -0.5 347,330 - +0.0 347,371 + +0.7 347,399 + +0.4 347,399 + -0.9 347,399 + -0.4 347,400 - +0.0 347,400 - +0.6 347,400 - -1.2 347,400 - +1.7 347,403 + +0.0 347,403 + +2.4 347,403 + -1.5 347,403 + +1.5 347,404 - -0.2 347,611 + +0.8 347,713 + +0.6 347,713 + +0.8 347,713 + -1.7 347,714 - -0.1 347,826 + Pf6N2E2_281 0.22 +0.1 347,827 - Pf6N2E2_281 0.23 -0.7 347,827 - Pf6N2E2_281 0.23 +1.6 347,827 - +1.4 347,911 + Pf6N2E2_281 0.34 +1.6 347,912 - Pf6N2E2_281 0.34 -1.7 347,912 - Pf6N2E2_281 0.34 +0.8 347,962 + Pf6N2E2_281 0.40 +0.2 347,962 + Pf6N2E2_281 0.40 -0.7 347,962 + Pf6N2E2_281 0.40 -2.9 347,962 + Pf6N2E2_281 0.40 -1.1 347,963 - Pf6N2E2_281 0.40 +0.8 347,963 - Pf6N2E2_281 0.40 +0.2 347,963 - Pf6N2E2_281 0.40 -1.3 348,165 - Pf6N2E2_281 0.67 +1.0
Or see this region's nucleotide sequence