Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2512 and Pf6N2E2_2513 are separated by 42 nucleotides Pf6N2E2_2513 and Pf6N2E2_2514 are separated by 168 nucleotides
Pf6N2E2_2512 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 2,963,455 to 2,964,477
_2512
Pf6N2E2_2513 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 2,964,520 to 2,965,803
_2513
Pf6N2E2_2514 - regulator of length of O-antigen component of lipopolysaccharide chains, at 2,965,972 to 2,967,021
_2514
Position (kb)
2964
2965
2966 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2964.245 kb on - strand, within Pf6N2E2_2512 at 2964.391 kb on - strand at 2964.398 kb on - strand at 2964.654 kb on + strand, within Pf6N2E2_2513 at 2964.760 kb on - strand, within Pf6N2E2_2513 at 2964.831 kb on + strand, within Pf6N2E2_2513 at 2964.842 kb on - strand, within Pf6N2E2_2513 at 2964.869 kb on - strand, within Pf6N2E2_2513 at 2964.912 kb on - strand, within Pf6N2E2_2513 at 2964.996 kb on - strand, within Pf6N2E2_2513 at 2965.015 kb on - strand, within Pf6N2E2_2513 at 2965.196 kb on - strand, within Pf6N2E2_2513 at 2965.713 kb on - strand at 2965.862 kb on - strand at 2965.865 kb on - strand at 2965.865 kb on - strand at 2965.885 kb on - strand at 2965.885 kb on - strand at 2965.939 kb on - strand at 2965.944 kb on - strand at 2966.008 kb on - strand at 2966.057 kb on - strand at 2966.108 kb on - strand, within Pf6N2E2_2514 at 2966.305 kb on - strand, within Pf6N2E2_2514 at 2966.344 kb on - strand, within Pf6N2E2_2514 at 2966.381 kb on - strand, within Pf6N2E2_2514 at 2966.479 kb on + strand, within Pf6N2E2_2514 at 2966.480 kb on - strand, within Pf6N2E2_2514 at 2966.491 kb on - strand, within Pf6N2E2_2514 at 2966.528 kb on + strand, within Pf6N2E2_2514 at 2966.585 kb on + strand, within Pf6N2E2_2514 at 2966.586 kb on - strand, within Pf6N2E2_2514 at 2966.693 kb on - strand, within Pf6N2E2_2514
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 2,964,245 - Pf6N2E2_2512 0.77 -0.1 2,964,391 - -0.9 2,964,398 - -0.4 2,964,654 + Pf6N2E2_2513 0.10 -0.7 2,964,760 - Pf6N2E2_2513 0.19 -0.9 2,964,831 + Pf6N2E2_2513 0.24 -2.4 2,964,842 - Pf6N2E2_2513 0.25 -1.5 2,964,869 - Pf6N2E2_2513 0.27 +0.0 2,964,912 - Pf6N2E2_2513 0.31 -2.7 2,964,996 - Pf6N2E2_2513 0.37 -0.8 2,965,015 - Pf6N2E2_2513 0.39 -1.0 2,965,196 - Pf6N2E2_2513 0.53 -2.4 2,965,713 - -1.1 2,965,862 - +1.3 2,965,865 - +0.2 2,965,865 - -0.4 2,965,885 - +1.4 2,965,885 - -2.4 2,965,939 - -0.9 2,965,944 - -0.8 2,966,008 - +0.8 2,966,057 - -2.2 2,966,108 - Pf6N2E2_2514 0.13 -1.5 2,966,305 - Pf6N2E2_2514 0.32 -0.3 2,966,344 - Pf6N2E2_2514 0.35 +0.8 2,966,381 - Pf6N2E2_2514 0.39 -1.6 2,966,479 + Pf6N2E2_2514 0.48 -0.3 2,966,480 - Pf6N2E2_2514 0.48 -2.8 2,966,491 - Pf6N2E2_2514 0.49 -2.4 2,966,528 + Pf6N2E2_2514 0.53 -0.9 2,966,585 + Pf6N2E2_2514 0.58 -1.6 2,966,586 - Pf6N2E2_2514 0.58 -0.4 2,966,693 - Pf6N2E2_2514 0.69 -1.4
Or see this region's nucleotide sequence