Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2100 and Pf6N2E2_2101 are separated by 783 nucleotides Pf6N2E2_2101 and Pf6N2E2_2102 are separated by 62 nucleotides
Pf6N2E2_2100 - DNA topoisomerase III (EC 5.99.1.2), at 2,498,254 to 2,500,200
_2100
Pf6N2E2_2101 - hypothetical protein, at 2,500,984 to 2,501,202
_2101
Pf6N2E2_2102 - Glutaminase (EC 3.5.1.2) (from data) , at 2,501,265 to 2,502,173
_2102
Position (kb)
2500
2501
2502 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2500.204 kb on + strand at 2500.204 kb on + strand at 2500.204 kb on + strand at 2500.204 kb on + strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.205 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.408 kb on - strand at 2500.411 kb on + strand at 2500.412 kb on - strand at 2500.412 kb on - strand at 2500.425 kb on - strand at 2500.432 kb on - strand at 2500.494 kb on - strand at 2500.699 kb on + strand at 2500.700 kb on - strand at 2500.713 kb on - strand at 2500.732 kb on + strand at 2500.732 kb on + strand at 2500.732 kb on + strand at 2500.732 kb on + strand at 2500.733 kb on - strand at 2500.733 kb on - strand at 2500.733 kb on - strand at 2500.805 kb on + strand at 2500.805 kb on + strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.806 kb on - strand at 2500.809 kb on + strand at 2500.809 kb on + strand at 2500.810 kb on - strand at 2500.810 kb on - strand at 2500.868 kb on + strand at 2500.868 kb on + strand at 2500.868 kb on + strand at 2500.868 kb on + strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.869 kb on - strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.896 kb on + strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.897 kb on - strand at 2500.916 kb on + strand at 2500.917 kb on - strand at 2500.950 kb on + strand at 2500.983 kb on + strand at 2501.002 kb on + strand at 2501.062 kb on + strand, within Pf6N2E2_2101 at 2501.063 kb on - strand, within Pf6N2E2_2101 at 2501.080 kb on + strand, within Pf6N2E2_2101 at 2501.080 kb on + strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.081 kb on - strand, within Pf6N2E2_2101 at 2501.266 kb on + strand at 2501.267 kb on - strand at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.462 kb on - strand, within Pf6N2E2_2102 at 2501.617 kb on + strand, within Pf6N2E2_2102 at 2501.617 kb on + strand, within Pf6N2E2_2102 at 2501.674 kb on + strand, within Pf6N2E2_2102 at 2501.674 kb on + strand, within Pf6N2E2_2102 at 2501.675 kb on - strand, within Pf6N2E2_2102 at 2501.675 kb on - strand, within Pf6N2E2_2102 at 2501.757 kb on + strand, within Pf6N2E2_2102 at 2501.758 kb on - strand, within Pf6N2E2_2102 at 2501.847 kb on - strand, within Pf6N2E2_2102 at 2501.847 kb on - strand, within Pf6N2E2_2102 at 2501.991 kb on - strand, within Pf6N2E2_2102 at 2502.052 kb on + strand, within Pf6N2E2_2102 at 2502.173 kb on + strand at 2502.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 2,500,204 + -0.0 2,500,204 + +1.0 2,500,204 + -2.0 2,500,204 + -0.4 2,500,205 - +0.8 2,500,205 - +1.2 2,500,205 - -1.2 2,500,205 - -0.1 2,500,205 - +0.7 2,500,205 - +0.6 2,500,205 - -0.7 2,500,205 - +0.2 2,500,205 - +0.7 2,500,408 - +1.3 2,500,408 - -1.4 2,500,408 - -0.0 2,500,408 - +0.8 2,500,408 - -1.5 2,500,411 + -0.4 2,500,412 - +0.4 2,500,412 - +0.3 2,500,425 - -1.3 2,500,432 - +0.8 2,500,494 - -0.5 2,500,699 + +0.4 2,500,700 - -0.2 2,500,713 - +0.8 2,500,732 + +1.2 2,500,732 + +0.8 2,500,732 + -0.1 2,500,732 + -0.1 2,500,733 - -0.4 2,500,733 - -0.8 2,500,733 - +1.3 2,500,805 + -1.3 2,500,805 + +2.0 2,500,806 - -0.4 2,500,806 - +0.4 2,500,806 - +0.7 2,500,806 - +0.5 2,500,806 - -2.5 2,500,809 + -1.4 2,500,809 + -0.7 2,500,810 - -0.5 2,500,810 - -1.0 2,500,868 + +0.0 2,500,868 + +0.2 2,500,868 + +0.6 2,500,868 + -0.4 2,500,869 - -1.5 2,500,869 - -0.7 2,500,869 - -1.4 2,500,869 - +1.2 2,500,869 - +0.9 2,500,896 + -0.5 2,500,896 + -1.0 2,500,896 + +0.6 2,500,896 + +0.4 2,500,896 + -1.5 2,500,896 + -0.2 2,500,896 + +0.5 2,500,896 + +1.3 2,500,896 + -1.0 2,500,896 + +0.1 2,500,897 - -0.3 2,500,897 - -0.2 2,500,897 - -0.7 2,500,897 - +0.0 2,500,897 - -1.2 2,500,897 - +0.8 2,500,897 - -1.3 2,500,897 - -0.2 2,500,897 - -0.7 2,500,916 + -0.8 2,500,917 - -0.5 2,500,950 + -1.4 2,500,983 + +0.8 2,501,002 + +0.7 2,501,062 + Pf6N2E2_2101 0.36 +0.1 2,501,063 - Pf6N2E2_2101 0.36 -0.5 2,501,080 + Pf6N2E2_2101 0.44 +0.3 2,501,080 + Pf6N2E2_2101 0.44 -0.1 2,501,081 - Pf6N2E2_2101 0.44 +0.8 2,501,081 - Pf6N2E2_2101 0.44 +1.5 2,501,081 - Pf6N2E2_2101 0.44 +0.9 2,501,081 - Pf6N2E2_2101 0.44 +1.3 2,501,081 - Pf6N2E2_2101 0.44 +0.8 2,501,081 - Pf6N2E2_2101 0.44 +0.7 2,501,266 + +0.8 2,501,267 - +0.5 2,501,462 - Pf6N2E2_2102 0.22 -0.2 2,501,462 - Pf6N2E2_2102 0.22 -1.1 2,501,462 - Pf6N2E2_2102 0.22 +0.7 2,501,462 - Pf6N2E2_2102 0.22 +0.8 2,501,617 + Pf6N2E2_2102 0.39 -0.5 2,501,617 + Pf6N2E2_2102 0.39 +1.6 2,501,674 + Pf6N2E2_2102 0.45 -0.2 2,501,674 + Pf6N2E2_2102 0.45 +0.2 2,501,675 - Pf6N2E2_2102 0.45 +0.1 2,501,675 - Pf6N2E2_2102 0.45 -0.6 2,501,757 + Pf6N2E2_2102 0.54 +1.4 2,501,758 - Pf6N2E2_2102 0.54 -2.2 2,501,847 - Pf6N2E2_2102 0.64 -0.1 2,501,847 - Pf6N2E2_2102 0.64 +0.4 2,501,991 - Pf6N2E2_2102 0.80 +0.3 2,502,052 + Pf6N2E2_2102 0.87 +0.8 2,502,173 + +0.4 2,502,173 + +1.1
Or see this region's nucleotide sequence