Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2086 and Pf6N2E2_2087 are separated by 7 nucleotides Pf6N2E2_2087 and Pf6N2E2_2088 overlap by 1 nucleotides Pf6N2E2_2088 and Pf6N2E2_2089 are separated by 80 nucleotides
Pf6N2E2_2086 - DNA polymerase-like protein PA0670, at 2,481,739 to 2,483,160
_2086
Pf6N2E2_2087 - RecA/RadA recombinase, at 2,483,168 to 2,483,800
_2087
Pf6N2E2_2088 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 2,483,800 to 2,484,417
_2088
Pf6N2E2_2089 - Transcriptional regulator, AraC family, at 2,484,498 to 2,485,541
_2089
Position (kb)
2483
2484
2485 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2482.858 kb on - strand, within Pf6N2E2_2086 at 2482.858 kb on - strand, within Pf6N2E2_2086 at 2482.858 kb on - strand, within Pf6N2E2_2086 at 2482.947 kb on - strand, within Pf6N2E2_2086 at 2483.074 kb on + strand at 2483.075 kb on - strand at 2483.145 kb on + strand at 2483.145 kb on + strand at 2483.146 kb on - strand at 2483.837 kb on - strand at 2484.065 kb on + strand, within Pf6N2E2_2088 at 2484.065 kb on + strand, within Pf6N2E2_2088 at 2484.998 kb on - strand, within Pf6N2E2_2089 at 2484.998 kb on - strand, within Pf6N2E2_2089 at 2484.998 kb on - strand, within Pf6N2E2_2089 at 2484.998 kb on - strand, within Pf6N2E2_2089 at 2485.066 kb on + strand, within Pf6N2E2_2089 at 2485.067 kb on - strand, within Pf6N2E2_2089 at 2485.067 kb on - strand, within Pf6N2E2_2089 at 2485.067 kb on - strand, within Pf6N2E2_2089 at 2485.254 kb on + strand, within Pf6N2E2_2089 at 2485.255 kb on - strand, within Pf6N2E2_2089 at 2485.255 kb on - strand, within Pf6N2E2_2089
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 2,482,858 - Pf6N2E2_2086 0.79 -2.3 2,482,858 - Pf6N2E2_2086 0.79 -1.4 2,482,858 - Pf6N2E2_2086 0.79 +1.2 2,482,947 - Pf6N2E2_2086 0.85 -0.4 2,483,074 + +0.4 2,483,075 - -0.0 2,483,145 + -1.2 2,483,145 + -2.2 2,483,146 - +0.4 2,483,837 - +0.4 2,484,065 + Pf6N2E2_2088 0.43 -1.2 2,484,065 + Pf6N2E2_2088 0.43 -2.2 2,484,998 - Pf6N2E2_2089 0.48 +1.2 2,484,998 - Pf6N2E2_2089 0.48 -0.6 2,484,998 - Pf6N2E2_2089 0.48 +1.8 2,484,998 - Pf6N2E2_2089 0.48 +0.3 2,485,066 + Pf6N2E2_2089 0.54 -1.1 2,485,067 - Pf6N2E2_2089 0.55 -0.8 2,485,067 - Pf6N2E2_2089 0.55 +0.2 2,485,067 - Pf6N2E2_2089 0.55 -0.1 2,485,254 + Pf6N2E2_2089 0.72 +1.6 2,485,255 - Pf6N2E2_2089 0.73 +0.2 2,485,255 - Pf6N2E2_2089 0.73 -1.1
Or see this region's nucleotide sequence