Experiment: dimethyl sulfone 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2059 and Pf6N2E2_2060 are separated by 174 nucleotides Pf6N2E2_2060 and Pf6N2E2_2061 are separated by 3 nucleotides
Pf6N2E2_2059 - ChlI component of cobalt chelatase involved in B12 biosynthesis, at 2,448,304 to 2,449,308
_2059
Pf6N2E2_2060 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 2,449,483 to 2,453,256
_2060
Pf6N2E2_2061 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 2,453,260 to 2,454,324
_2061
Position (kb)
2449
2450
2451
2452
2453
2454 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2449.001 kb on + strand, within Pf6N2E2_2059 at 2449.001 kb on + strand, within Pf6N2E2_2059 at 2449.002 kb on - strand, within Pf6N2E2_2059 at 2449.002 kb on - strand, within Pf6N2E2_2059 at 2449.652 kb on + strand at 2449.653 kb on - strand at 2449.653 kb on - strand at 2449.673 kb on + strand at 2449.673 kb on + strand at 2449.757 kb on + strand at 2449.758 kb on - strand at 2449.880 kb on + strand, within Pf6N2E2_2060 at 2449.880 kb on + strand, within Pf6N2E2_2060 at 2449.881 kb on - strand, within Pf6N2E2_2060 at 2449.881 kb on - strand, within Pf6N2E2_2060 at 2449.943 kb on + strand, within Pf6N2E2_2060 at 2449.943 kb on + strand, within Pf6N2E2_2060 at 2449.943 kb on + strand, within Pf6N2E2_2060 at 2449.943 kb on + strand, within Pf6N2E2_2060 at 2449.943 kb on + strand, within Pf6N2E2_2060 at 2449.944 kb on - strand, within Pf6N2E2_2060 at 2449.944 kb on - strand, within Pf6N2E2_2060 at 2449.946 kb on + strand, within Pf6N2E2_2060 at 2449.947 kb on - strand, within Pf6N2E2_2060 at 2450.423 kb on - strand, within Pf6N2E2_2060 at 2451.163 kb on - strand, within Pf6N2E2_2060 at 2451.206 kb on + strand, within Pf6N2E2_2060 at 2451.206 kb on + strand, within Pf6N2E2_2060 at 2451.206 kb on + strand, within Pf6N2E2_2060 at 2451.206 kb on + strand, within Pf6N2E2_2060 at 2451.206 kb on + strand, within Pf6N2E2_2060 at 2451.207 kb on - strand, within Pf6N2E2_2060 at 2451.362 kb on + strand, within Pf6N2E2_2060 at 2451.362 kb on + strand, within Pf6N2E2_2060 at 2451.362 kb on + strand, within Pf6N2E2_2060 at 2451.362 kb on + strand, within Pf6N2E2_2060 at 2451.363 kb on - strand, within Pf6N2E2_2060 at 2451.363 kb on - strand, within Pf6N2E2_2060 at 2451.363 kb on - strand, within Pf6N2E2_2060 at 2451.404 kb on + strand, within Pf6N2E2_2060 at 2451.404 kb on + strand, within Pf6N2E2_2060 at 2451.405 kb on - strand, within Pf6N2E2_2060 at 2451.405 kb on - strand, within Pf6N2E2_2060 at 2451.509 kb on + strand, within Pf6N2E2_2060 at 2451.509 kb on + strand, within Pf6N2E2_2060 at 2451.509 kb on + strand, within Pf6N2E2_2060 at 2451.510 kb on - strand, within Pf6N2E2_2060 at 2451.510 kb on - strand, within Pf6N2E2_2060 at 2451.510 kb on - strand, within Pf6N2E2_2060 at 2451.632 kb on + strand, within Pf6N2E2_2060 at 2451.633 kb on - strand, within Pf6N2E2_2060 at 2451.653 kb on + strand, within Pf6N2E2_2060 at 2451.653 kb on + strand, within Pf6N2E2_2060 at 2451.698 kb on + strand, within Pf6N2E2_2060 at 2451.722 kb on + strand, within Pf6N2E2_2060 at 2452.100 kb on + strand, within Pf6N2E2_2060 at 2452.101 kb on - strand, within Pf6N2E2_2060 at 2452.101 kb on - strand, within Pf6N2E2_2060 at 2452.464 kb on + strand, within Pf6N2E2_2060 at 2452.638 kb on - strand, within Pf6N2E2_2060 at 2452.721 kb on + strand, within Pf6N2E2_2060 at 2452.721 kb on + strand, within Pf6N2E2_2060 at 2452.721 kb on + strand, within Pf6N2E2_2060 at 2452.722 kb on - strand, within Pf6N2E2_2060 at 2452.722 kb on - strand, within Pf6N2E2_2060 at 2452.764 kb on - strand, within Pf6N2E2_2060 at 2453.046 kb on - strand at 2453.046 kb on - strand at 2453.261 kb on + strand at 2453.459 kb on - strand, within Pf6N2E2_2061 at 2453.648 kb on - strand, within Pf6N2E2_2061 at 2454.021 kb on + strand, within Pf6N2E2_2061 at 2454.022 kb on - strand, within Pf6N2E2_2061 at 2454.022 kb on - strand, within Pf6N2E2_2061 at 2454.116 kb on + strand, within Pf6N2E2_2061 at 2454.116 kb on + strand, within Pf6N2E2_2061 at 2454.116 kb on + strand, within Pf6N2E2_2061 at 2454.117 kb on - strand, within Pf6N2E2_2061
Per-strain Table
Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M remove 2,449,001 + Pf6N2E2_2059 0.69 +0.8 2,449,001 + Pf6N2E2_2059 0.69 -1.0 2,449,002 - Pf6N2E2_2059 0.69 +0.1 2,449,002 - Pf6N2E2_2059 0.69 +0.1 2,449,652 + +0.7 2,449,653 - -2.3 2,449,653 - -0.2 2,449,673 + -0.2 2,449,673 + +0.1 2,449,757 + +0.3 2,449,758 - +1.2 2,449,880 + Pf6N2E2_2060 0.11 -1.2 2,449,880 + Pf6N2E2_2060 0.11 -0.6 2,449,881 - Pf6N2E2_2060 0.11 -1.6 2,449,881 - Pf6N2E2_2060 0.11 +0.7 2,449,943 + Pf6N2E2_2060 0.12 -0.9 2,449,943 + Pf6N2E2_2060 0.12 +0.2 2,449,943 + Pf6N2E2_2060 0.12 +1.7 2,449,943 + Pf6N2E2_2060 0.12 -1.8 2,449,943 + Pf6N2E2_2060 0.12 -1.1 2,449,944 - Pf6N2E2_2060 0.12 -1.5 2,449,944 - Pf6N2E2_2060 0.12 -1.9 2,449,946 + Pf6N2E2_2060 0.12 +0.1 2,449,947 - Pf6N2E2_2060 0.12 -1.5 2,450,423 - Pf6N2E2_2060 0.25 -1.3 2,451,163 - Pf6N2E2_2060 0.45 -0.2 2,451,206 + Pf6N2E2_2060 0.46 -0.3 2,451,206 + Pf6N2E2_2060 0.46 -1.7 2,451,206 + Pf6N2E2_2060 0.46 +0.1 2,451,206 + Pf6N2E2_2060 0.46 -1.3 2,451,206 + Pf6N2E2_2060 0.46 -1.8 2,451,207 - Pf6N2E2_2060 0.46 +1.1 2,451,362 + Pf6N2E2_2060 0.50 -0.2 2,451,362 + Pf6N2E2_2060 0.50 -0.3 2,451,362 + Pf6N2E2_2060 0.50 -1.3 2,451,362 + Pf6N2E2_2060 0.50 +1.4 2,451,363 - Pf6N2E2_2060 0.50 -0.2 2,451,363 - Pf6N2E2_2060 0.50 -1.0 2,451,363 - Pf6N2E2_2060 0.50 -0.6 2,451,404 + Pf6N2E2_2060 0.51 -1.7 2,451,404 + Pf6N2E2_2060 0.51 -1.8 2,451,405 - Pf6N2E2_2060 0.51 -2.3 2,451,405 - Pf6N2E2_2060 0.51 -1.9 2,451,509 + Pf6N2E2_2060 0.54 -2.4 2,451,509 + Pf6N2E2_2060 0.54 -1.5 2,451,509 + Pf6N2E2_2060 0.54 -2.6 2,451,510 - Pf6N2E2_2060 0.54 -2.0 2,451,510 - Pf6N2E2_2060 0.54 -0.9 2,451,510 - Pf6N2E2_2060 0.54 -1.3 2,451,632 + Pf6N2E2_2060 0.57 -0.5 2,451,633 - Pf6N2E2_2060 0.57 -0.6 2,451,653 + Pf6N2E2_2060 0.57 +0.2 2,451,653 + Pf6N2E2_2060 0.57 -1.5 2,451,698 + Pf6N2E2_2060 0.59 +1.0 2,451,722 + Pf6N2E2_2060 0.59 -2.8 2,452,100 + Pf6N2E2_2060 0.69 +0.4 2,452,101 - Pf6N2E2_2060 0.69 -0.3 2,452,101 - Pf6N2E2_2060 0.69 +0.3 2,452,464 + Pf6N2E2_2060 0.79 +0.5 2,452,638 - Pf6N2E2_2060 0.84 +1.4 2,452,721 + Pf6N2E2_2060 0.86 -0.4 2,452,721 + Pf6N2E2_2060 0.86 -1.1 2,452,721 + Pf6N2E2_2060 0.86 -0.4 2,452,722 - Pf6N2E2_2060 0.86 -0.3 2,452,722 - Pf6N2E2_2060 0.86 -1.6 2,452,764 - Pf6N2E2_2060 0.87 -1.1 2,453,046 - -0.6 2,453,046 - -0.1 2,453,261 + +0.1 2,453,459 - Pf6N2E2_2061 0.19 -1.1 2,453,648 - Pf6N2E2_2061 0.36 -0.1 2,454,021 + Pf6N2E2_2061 0.71 -0.4 2,454,022 - Pf6N2E2_2061 0.72 +0.2 2,454,022 - Pf6N2E2_2061 0.72 -0.3 2,454,116 + Pf6N2E2_2061 0.80 -1.5 2,454,116 + Pf6N2E2_2061 0.80 -1.9 2,454,116 + Pf6N2E2_2061 0.80 -0.7 2,454,117 - Pf6N2E2_2061 0.80 -0.6
Or see this region's nucleotide sequence