Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1077

Experiment: dimethyl sulfone 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1076 and Pf6N2E2_1077 overlap by 4 nucleotidesPf6N2E2_1077 and Pf6N2E2_1078 are separated by 49 nucleotides Pf6N2E2_1076 - 4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25), at 1,257,423 to 1,259,501 _1076 Pf6N2E2_1077 - Malto-oligosyltrehalose trehalohydrolase (EC 3.2.1.141), at 1,259,498 to 1,261,258 _1077 Pf6N2E2_1078 - Glycogen synthase, ADP-glucose transglucosylase (EC 2.4.1.21), at 1,261,308 to 1,262,879 _1078 Position (kb) 1259 1260 1261 1262Strain fitness (log2 ratio) -3 -2 -1 0 1at 1258.874 kb on - strand, within Pf6N2E2_1076at 1258.874 kb on - strand, within Pf6N2E2_1076at 1259.023 kb on + strand, within Pf6N2E2_1076at 1259.023 kb on + strand, within Pf6N2E2_1076at 1259.024 kb on - strand, within Pf6N2E2_1076at 1259.024 kb on - strand, within Pf6N2E2_1076at 1259.024 kb on - strand, within Pf6N2E2_1076at 1259.087 kb on - strand, within Pf6N2E2_1076at 1259.251 kb on + strand, within Pf6N2E2_1076at 1259.252 kb on - strand, within Pf6N2E2_1076at 1259.252 kb on - strand, within Pf6N2E2_1076at 1260.005 kb on - strand, within Pf6N2E2_1077at 1260.071 kb on + strand, within Pf6N2E2_1077at 1260.307 kb on - strand, within Pf6N2E2_1077at 1260.315 kb on + strand, within Pf6N2E2_1077at 1260.315 kb on + strand, within Pf6N2E2_1077at 1260.318 kb on + strand, within Pf6N2E2_1077at 1260.319 kb on - strand, within Pf6N2E2_1077at 1260.387 kb on + strand, within Pf6N2E2_1077at 1260.387 kb on + strand, within Pf6N2E2_1077at 1260.388 kb on - strand, within Pf6N2E2_1077at 1260.519 kb on - strand, within Pf6N2E2_1077at 1260.549 kb on + strand, within Pf6N2E2_1077at 1260.550 kb on - strand, within Pf6N2E2_1077at 1260.678 kb on + strand, within Pf6N2E2_1077at 1260.678 kb on + strand, within Pf6N2E2_1077at 1260.678 kb on + strand, within Pf6N2E2_1077at 1260.678 kb on + strand, within Pf6N2E2_1077at 1260.678 kb on + strand, within Pf6N2E2_1077at 1260.678 kb on + strand, within Pf6N2E2_1077at 1260.705 kb on + strand, within Pf6N2E2_1077at 1260.705 kb on + strand, within Pf6N2E2_1077at 1260.705 kb on + strand, within Pf6N2E2_1077at 1260.705 kb on + strand, within Pf6N2E2_1077at 1260.706 kb on - strand, within Pf6N2E2_1077at 1261.005 kb on + strand, within Pf6N2E2_1077at 1261.101 kb on + strandat 1261.102 kb on - strandat 1261.304 kb on + strandat 1261.305 kb on - strandat 1261.337 kb on + strandat 1261.337 kb on + strandat 1261.355 kb on - strandat 1261.355 kb on - strandat 1261.366 kb on + strandat 1261.366 kb on + strandat 1261.481 kb on - strand, within Pf6N2E2_1078at 1261.481 kb on - strand, within Pf6N2E2_1078at 1261.591 kb on + strand, within Pf6N2E2_1078at 1261.592 kb on - strand, within Pf6N2E2_1078at 1261.592 kb on - strand, within Pf6N2E2_1078at 1262.095 kb on + strand, within Pf6N2E2_1078at 1262.095 kb on + strand, within Pf6N2E2_1078at 1262.095 kb on + strand, within Pf6N2E2_1078at 1262.095 kb on + strand, within Pf6N2E2_1078at 1262.095 kb on + strand, within Pf6N2E2_1078at 1262.096 kb on - strand, within Pf6N2E2_1078at 1262.096 kb on - strand, within Pf6N2E2_1078at 1262.096 kb on - strand, within Pf6N2E2_1078at 1262.117 kb on + strand, within Pf6N2E2_1078at 1262.117 kb on + strand, within Pf6N2E2_1078at 1262.117 kb on + strand, within Pf6N2E2_1078at 1262.128 kb on + strand, within Pf6N2E2_1078at 1262.128 kb on + strand, within Pf6N2E2_1078at 1262.129 kb on - strand, within Pf6N2E2_1078at 1262.129 kb on - strand, within Pf6N2E2_1078at 1262.165 kb on - strand, within Pf6N2E2_1078

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Per-strain Table

Position Strand Gene LocusTag Fraction dimethyl sulfone 0.25 M
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1,258,874 - Pf6N2E2_1076 0.70 -0.0
1,258,874 - Pf6N2E2_1076 0.70 -0.7
1,259,023 + Pf6N2E2_1076 0.77 -0.8
1,259,023 + Pf6N2E2_1076 0.77 -0.6
1,259,024 - Pf6N2E2_1076 0.77 -1.4
1,259,024 - Pf6N2E2_1076 0.77 +0.8
1,259,024 - Pf6N2E2_1076 0.77 +0.8
1,259,087 - Pf6N2E2_1076 0.80 +0.6
1,259,251 + Pf6N2E2_1076 0.88 -1.6
1,259,252 - Pf6N2E2_1076 0.88 -1.2
1,259,252 - Pf6N2E2_1076 0.88 +0.4
1,260,005 - Pf6N2E2_1077 0.29 -0.4
1,260,071 + Pf6N2E2_1077 0.33 -0.3
1,260,307 - Pf6N2E2_1077 0.46 +1.4
1,260,315 + Pf6N2E2_1077 0.46 -0.4
1,260,315 + Pf6N2E2_1077 0.46 -0.8
1,260,318 + Pf6N2E2_1077 0.47 -0.2
1,260,319 - Pf6N2E2_1077 0.47 +0.1
1,260,387 + Pf6N2E2_1077 0.50 -0.9
1,260,387 + Pf6N2E2_1077 0.50 -0.9
1,260,388 - Pf6N2E2_1077 0.51 +0.2
1,260,519 - Pf6N2E2_1077 0.58 -3.0
1,260,549 + Pf6N2E2_1077 0.60 +1.5
1,260,550 - Pf6N2E2_1077 0.60 +0.1
1,260,678 + Pf6N2E2_1077 0.67 -1.8
1,260,678 + Pf6N2E2_1077 0.67 -1.1
1,260,678 + Pf6N2E2_1077 0.67 -0.3
1,260,678 + Pf6N2E2_1077 0.67 -1.0
1,260,678 + Pf6N2E2_1077 0.67 -1.0
1,260,678 + Pf6N2E2_1077 0.67 -0.6
1,260,705 + Pf6N2E2_1077 0.69 -0.0
1,260,705 + Pf6N2E2_1077 0.69 -0.9
1,260,705 + Pf6N2E2_1077 0.69 +0.3
1,260,705 + Pf6N2E2_1077 0.69 -0.5
1,260,706 - Pf6N2E2_1077 0.69 -0.9
1,261,005 + Pf6N2E2_1077 0.86 -1.3
1,261,101 + -0.1
1,261,102 - -0.8
1,261,304 + -0.6
1,261,305 - -0.6
1,261,337 + -1.3
1,261,337 + -0.2
1,261,355 - -1.9
1,261,355 - -2.1
1,261,366 + -1.5
1,261,366 + -3.0
1,261,481 - Pf6N2E2_1078 0.11 -1.9
1,261,481 - Pf6N2E2_1078 0.11 -1.2
1,261,591 + Pf6N2E2_1078 0.18 -1.6
1,261,592 - Pf6N2E2_1078 0.18 +0.4
1,261,592 - Pf6N2E2_1078 0.18 -2.8
1,262,095 + Pf6N2E2_1078 0.50 -2.7
1,262,095 + Pf6N2E2_1078 0.50 +0.6
1,262,095 + Pf6N2E2_1078 0.50 -2.7
1,262,095 + Pf6N2E2_1078 0.50 -0.9
1,262,095 + Pf6N2E2_1078 0.50 -2.5
1,262,096 - Pf6N2E2_1078 0.50 -0.8
1,262,096 - Pf6N2E2_1078 0.50 +0.2
1,262,096 - Pf6N2E2_1078 0.50 -0.7
1,262,117 + Pf6N2E2_1078 0.51 -0.5
1,262,117 + Pf6N2E2_1078 0.51 -2.7
1,262,117 + Pf6N2E2_1078 0.51 -2.4
1,262,128 + Pf6N2E2_1078 0.52 -0.5
1,262,128 + Pf6N2E2_1078 0.52 -1.4
1,262,129 - Pf6N2E2_1078 0.52 +1.4
1,262,129 - Pf6N2E2_1078 0.52 +1.4
1,262,165 - Pf6N2E2_1078 0.55 -1.8

Or see this region's nucleotide sequence