Experiment: nitrate 0.125 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4 and Pf6N2E2_5 are separated by 11 nucleotides Pf6N2E2_5 and Pf6N2E2_6 overlap by 4 nucleotides
Pf6N2E2_4 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 14,454 to 20,954
_4
Pf6N2E2_5 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 20,966 to 27,997
_5
Pf6N2E2_6 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 27,994 to 31,221
_6
Position (kb) 20
21
22
23
24
25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1
2 at 20.028 kb on + strand, within Pf6N2E2_4 at 20.028 kb on + strand, within Pf6N2E2_4 at 20.029 kb on - strand, within Pf6N2E2_4 at 20.029 kb on - strand, within Pf6N2E2_4 at 20.038 kb on - strand, within Pf6N2E2_4 at 20.139 kb on + strand, within Pf6N2E2_4 at 20.139 kb on + strand, within Pf6N2E2_4 at 20.139 kb on + strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.140 kb on - strand, within Pf6N2E2_4 at 20.193 kb on + strand, within Pf6N2E2_4 at 20.194 kb on - strand, within Pf6N2E2_4 at 20.479 kb on - strand at 20.479 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.581 kb on - strand at 20.692 kb on + strand at 20.692 kb on + strand at 20.693 kb on - strand at 21.210 kb on + strand at 21.417 kb on - strand at 21.536 kb on + strand at 21.980 kb on - strand, within Pf6N2E2_5 at 22.020 kb on - strand, within Pf6N2E2_5 at 22.232 kb on + strand, within Pf6N2E2_5 at 22.233 kb on - strand, within Pf6N2E2_5 at 22.233 kb on - strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.376 kb on + strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.377 kb on - strand, within Pf6N2E2_5 at 22.442 kb on + strand, within Pf6N2E2_5 at 22.442 kb on + strand, within Pf6N2E2_5 at 22.442 kb on + strand, within Pf6N2E2_5 at 22.443 kb on - strand, within Pf6N2E2_5 at 22.757 kb on + strand, within Pf6N2E2_5 at 22.758 kb on - strand, within Pf6N2E2_5 at 22.871 kb on + strand, within Pf6N2E2_5 at 23.538 kb on - strand, within Pf6N2E2_5 at 23.538 kb on - strand, within Pf6N2E2_5 at 23.801 kb on + strand, within Pf6N2E2_5 at 23.802 kb on - strand, within Pf6N2E2_5 at 23.802 kb on - strand, within Pf6N2E2_5 at 23.991 kb on - strand, within Pf6N2E2_5 at 23.991 kb on - strand, within Pf6N2E2_5 at 23.991 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.162 kb on - strand, within Pf6N2E2_5 at 24.170 kb on + strand, within Pf6N2E2_5 at 24.171 kb on - strand, within Pf6N2E2_5 at 24.257 kb on + strand, within Pf6N2E2_5 at 24.584 kb on + strand, within Pf6N2E2_5 at 24.584 kb on + strand, within Pf6N2E2_5 at 24.585 kb on - strand, within Pf6N2E2_5 at 24.585 kb on - strand, within Pf6N2E2_5 at 24.585 kb on - strand, within Pf6N2E2_5 at 24.691 kb on + strand, within Pf6N2E2_5 at 24.692 kb on - strand, within Pf6N2E2_5 at 24.698 kb on - strand, within Pf6N2E2_5 at 24.750 kb on - strand, within Pf6N2E2_5 at 25.136 kb on + strand, within Pf6N2E2_5 at 25.136 kb on + strand, within Pf6N2E2_5 at 25.136 kb on + strand, within Pf6N2E2_5 at 25.137 kb on - strand, within Pf6N2E2_5 at 25.137 kb on - strand, within Pf6N2E2_5 at 25.137 kb on - strand, within Pf6N2E2_5 at 25.303 kb on - strand, within Pf6N2E2_5 at 25.311 kb on + strand, within Pf6N2E2_5 at 25.311 kb on + strand, within Pf6N2E2_5 at 25.406 kb on + strand, within Pf6N2E2_5 at 25.613 kb on + strand, within Pf6N2E2_5 at 25.718 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.937 kb on + strand, within Pf6N2E2_5 at 25.938 kb on - strand, within Pf6N2E2_5 at 26.060 kb on + strand, within Pf6N2E2_5 at 26.061 kb on - strand, within Pf6N2E2_5 at 26.063 kb on + strand, within Pf6N2E2_5 at 26.063 kb on + strand, within Pf6N2E2_5 at 26.151 kb on - strand, within Pf6N2E2_5 at 26.204 kb on + strand, within Pf6N2E2_5 at 26.205 kb on - strand, within Pf6N2E2_5 at 26.205 kb on - strand, within Pf6N2E2_5 at 26.205 kb on - strand, within Pf6N2E2_5 at 26.291 kb on + strand, within Pf6N2E2_5 at 26.291 kb on + strand, within Pf6N2E2_5 at 26.291 kb on + strand, within Pf6N2E2_5 at 26.614 kb on - strand, within Pf6N2E2_5 at 26.614 kb on - strand, within Pf6N2E2_5 at 26.681 kb on - strand, within Pf6N2E2_5 at 27.233 kb on + strand, within Pf6N2E2_5 at 27.234 kb on - strand, within Pf6N2E2_5 at 27.323 kb on + strand at 27.323 kb on + strand at 27.324 kb on - strand at 27.324 kb on - strand at 27.383 kb on + strand at 27.656 kb on + strand at 27.675 kb on + strand at 27.676 kb on - strand at 27.728 kb on + strand at 27.728 kb on + strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.731 kb on + strand at 27.732 kb on - strand at 27.785 kb on + strand at 27.785 kb on + strand at 27.785 kb on + strand at 27.786 kb on - strand at 27.910 kb on + strand at 27.910 kb on + strand at 27.910 kb on + strand at 27.911 kb on - strand at 27.911 kb on - strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.915 kb on - strand at 28.170 kb on - strand at 28.373 kb on + strand, within Pf6N2E2_6 at 28.373 kb on + strand, within Pf6N2E2_6 at 28.373 kb on + strand, within Pf6N2E2_6 at 28.376 kb on - strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.467 kb on - strand, within Pf6N2E2_6 at 28.477 kb on + strand, within Pf6N2E2_6 at 28.477 kb on + strand, within Pf6N2E2_6 at 28.478 kb on - strand, within Pf6N2E2_6 at 28.478 kb on - strand, within Pf6N2E2_6 at 28.529 kb on - strand, within Pf6N2E2_6 at 28.586 kb on - strand, within Pf6N2E2_6 at 28.858 kb on + strand, within Pf6N2E2_6 at 28.858 kb on + strand, within Pf6N2E2_6 at 28.859 kb on - strand, within Pf6N2E2_6
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 0.125 M remove 20,028 + Pf6N2E2_4 0.86 +0.4 20,028 + Pf6N2E2_4 0.86 +0.4 20,029 - Pf6N2E2_4 0.86 +0.8 20,029 - Pf6N2E2_4 0.86 +0.6 20,038 - Pf6N2E2_4 0.86 +0.3 20,139 + Pf6N2E2_4 0.87 +1.2 20,139 + Pf6N2E2_4 0.87 +2.0 20,139 + Pf6N2E2_4 0.87 +0.6 20,140 - Pf6N2E2_4 0.87 -0.3 20,140 - Pf6N2E2_4 0.87 +0.7 20,140 - Pf6N2E2_4 0.87 +0.4 20,140 - Pf6N2E2_4 0.87 -1.7 20,140 - Pf6N2E2_4 0.87 -0.6 20,193 + Pf6N2E2_4 0.88 +0.1 20,194 - Pf6N2E2_4 0.88 -0.7 20,479 - +1.1 20,479 - +1.5 20,581 - -1.2 20,581 - -1.0 20,581 - +0.1 20,581 - +0.2 20,581 - +2.0 20,692 + -0.3 20,692 + -0.8 20,693 - +0.1 21,210 + +0.2 21,417 - -0.5 21,536 + -0.8 21,980 - Pf6N2E2_5 0.14 +0.7 22,020 - Pf6N2E2_5 0.15 -0.2 22,232 + Pf6N2E2_5 0.18 -0.9 22,233 - Pf6N2E2_5 0.18 +0.6 22,233 - Pf6N2E2_5 0.18 -0.7 22,376 + Pf6N2E2_5 0.20 -0.5 22,376 + Pf6N2E2_5 0.20 +0.0 22,376 + Pf6N2E2_5 0.20 +0.1 22,376 + Pf6N2E2_5 0.20 -0.1 22,376 + Pf6N2E2_5 0.20 -1.1 22,377 - Pf6N2E2_5 0.20 +1.3 22,377 - Pf6N2E2_5 0.20 +1.5 22,377 - Pf6N2E2_5 0.20 +0.3 22,377 - Pf6N2E2_5 0.20 -0.4 22,377 - Pf6N2E2_5 0.20 -1.1 22,442 + Pf6N2E2_5 0.21 +0.3 22,442 + Pf6N2E2_5 0.21 -1.5 22,442 + Pf6N2E2_5 0.21 -0.2 22,443 - Pf6N2E2_5 0.21 +0.1 22,757 + Pf6N2E2_5 0.25 +0.4 22,758 - Pf6N2E2_5 0.25 -0.8 22,871 + Pf6N2E2_5 0.27 -0.3 23,538 - Pf6N2E2_5 0.37 -0.8 23,538 - Pf6N2E2_5 0.37 -0.0 23,801 + Pf6N2E2_5 0.40 -1.9 23,802 - Pf6N2E2_5 0.40 -0.3 23,802 - Pf6N2E2_5 0.40 +1.7 23,991 - Pf6N2E2_5 0.43 +0.2 23,991 - Pf6N2E2_5 0.43 -0.8 23,991 - Pf6N2E2_5 0.43 -0.4 24,162 - Pf6N2E2_5 0.45 -0.5 24,162 - Pf6N2E2_5 0.45 -1.3 24,162 - Pf6N2E2_5 0.45 +1.7 24,162 - Pf6N2E2_5 0.45 +1.0 24,170 + Pf6N2E2_5 0.46 +0.4 24,171 - Pf6N2E2_5 0.46 -1.3 24,257 + Pf6N2E2_5 0.47 +1.4 24,584 + Pf6N2E2_5 0.51 -0.3 24,584 + Pf6N2E2_5 0.51 +0.5 24,585 - Pf6N2E2_5 0.51 +1.0 24,585 - Pf6N2E2_5 0.51 +0.2 24,585 - Pf6N2E2_5 0.51 +0.5 24,691 + Pf6N2E2_5 0.53 -1.3 24,692 - Pf6N2E2_5 0.53 +0.4 24,698 - Pf6N2E2_5 0.53 -1.2 24,750 - Pf6N2E2_5 0.54 -0.0 25,136 + Pf6N2E2_5 0.59 -0.2 25,136 + Pf6N2E2_5 0.59 +0.4 25,136 + Pf6N2E2_5 0.59 -1.5 25,137 - Pf6N2E2_5 0.59 -1.5 25,137 - Pf6N2E2_5 0.59 +0.4 25,137 - Pf6N2E2_5 0.59 +0.8 25,303 - Pf6N2E2_5 0.62 -0.3 25,311 + Pf6N2E2_5 0.62 +0.1 25,311 + Pf6N2E2_5 0.62 -0.0 25,406 + Pf6N2E2_5 0.63 +1.2 25,613 + Pf6N2E2_5 0.66 -0.7 25,718 + Pf6N2E2_5 0.68 +0.1 25,937 + Pf6N2E2_5 0.71 -1.6 25,937 + Pf6N2E2_5 0.71 +0.5 25,937 + Pf6N2E2_5 0.71 +0.6 25,937 + Pf6N2E2_5 0.71 +0.4 25,937 + Pf6N2E2_5 0.71 +0.2 25,937 + Pf6N2E2_5 0.71 +0.4 25,937 + Pf6N2E2_5 0.71 +0.1 25,937 + Pf6N2E2_5 0.71 +1.3 25,937 + Pf6N2E2_5 0.71 +0.2 25,937 + Pf6N2E2_5 0.71 +0.7 25,938 - Pf6N2E2_5 0.71 +1.9 26,060 + Pf6N2E2_5 0.72 -1.6 26,061 - Pf6N2E2_5 0.72 +0.1 26,063 + Pf6N2E2_5 0.72 -0.6 26,063 + Pf6N2E2_5 0.72 +0.4 26,151 - Pf6N2E2_5 0.74 -0.8 26,204 + Pf6N2E2_5 0.74 +0.9 26,205 - Pf6N2E2_5 0.75 +0.4 26,205 - Pf6N2E2_5 0.75 +0.7 26,205 - Pf6N2E2_5 0.75 +0.7 26,291 + Pf6N2E2_5 0.76 +0.5 26,291 + Pf6N2E2_5 0.76 +0.2 26,291 + Pf6N2E2_5 0.76 +0.4 26,614 - Pf6N2E2_5 0.80 -1.7 26,614 - Pf6N2E2_5 0.80 -1.5 26,681 - Pf6N2E2_5 0.81 -0.7 27,233 + Pf6N2E2_5 0.89 +0.4 27,234 - Pf6N2E2_5 0.89 -0.9 27,323 + -0.3 27,323 + +0.8 27,324 - +0.1 27,324 - -1.3 27,383 + +0.7 27,656 + +0.4 27,675 + -0.8 27,676 - +1.5 27,728 + -0.3 27,728 + +0.0 27,729 - +0.7 27,729 - -0.7 27,729 - +0.7 27,729 - +0.7 27,729 - -0.3 27,731 + +0.4 27,732 - -0.8 27,785 + +1.4 27,785 + +0.6 27,785 + -0.2 27,786 - +0.2 27,910 + +0.7 27,910 + +0.1 27,910 + -0.2 27,911 - -0.6 27,911 - +0.8 27,914 + +1.4 27,914 + -0.3 27,914 + +0.1 27,914 + -1.9 27,914 + +0.6 27,914 + +0.2 27,914 + +0.9 27,914 + -2.7 27,915 - -1.6 28,170 - +0.7 28,373 + Pf6N2E2_6 0.12 -1.3 28,373 + Pf6N2E2_6 0.12 +1.3 28,373 + Pf6N2E2_6 0.12 -0.3 28,376 - Pf6N2E2_6 0.12 -1.9 28,466 + Pf6N2E2_6 0.15 +1.2 28,466 + Pf6N2E2_6 0.15 -2.3 28,466 + Pf6N2E2_6 0.15 +0.1 28,467 - Pf6N2E2_6 0.15 -0.9 28,477 + Pf6N2E2_6 0.15 +1.8 28,477 + Pf6N2E2_6 0.15 +0.1 28,478 - Pf6N2E2_6 0.15 +1.7 28,478 - Pf6N2E2_6 0.15 -1.0 28,529 - Pf6N2E2_6 0.17 -0.5 28,586 - Pf6N2E2_6 0.18 +0.1 28,858 + Pf6N2E2_6 0.27 -0.3 28,858 + Pf6N2E2_6 0.27 -1.0 28,859 - Pf6N2E2_6 0.27 +0.7
Or see this region's nucleotide sequence