Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01095 and PFLU_RS01100 are separated by 195 nucleotides PFLU_RS01100 and PFLU_RS30340 overlap by 20 nucleotides PFLU_RS30340 and PFLU_RS01110 are separated by 318 nucleotides
PFLU_RS01095: PFLU_RS01095 - ribbon-helix-helix protein, CopG family, at 242,916 to 243,188
_RS01095
PFLU_RS01100: PFLU_RS01100 - recombinase family protein, at 243,384 to 243,950
_RS01100
PFLU_RS30340: PFLU_RS30340 - hypothetical protein, at 243,931 to 244,169
_RS30340
PFLU_RS01110: PFLU_RS01110 - AAA family ATPase, at 244,488 to 245,112
_RS01110
Position (kb) 243
244
245 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 242.975 kb on + strand, within PFLU_RS01095 at 242.975 kb on + strand, within PFLU_RS01095 at 242.975 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.079 kb on + strand, within PFLU_RS01095 at 243.080 kb on - strand, within PFLU_RS01095 at 243.080 kb on - strand, within PFLU_RS01095 at 243.080 kb on - strand, within PFLU_RS01095 at 243.080 kb on - strand, within PFLU_RS01095 at 243.157 kb on - strand, within PFLU_RS01095 at 243.188 kb on + strand at 243.188 kb on + strand at 243.188 kb on + strand at 243.201 kb on - strand at 243.201 kb on - strand at 243.201 kb on - strand at 243.203 kb on + strand at 243.256 kb on + strand at 243.256 kb on + strand at 243.256 kb on + strand at 243.257 kb on - strand at 243.257 kb on - strand at 243.257 kb on - strand at 243.257 kb on - strand at 243.257 kb on - strand at 243.257 kb on - strand at 243.257 kb on - strand at 243.308 kb on + strand at 243.308 kb on + strand at 243.308 kb on + strand at 243.308 kb on + strand at 243.308 kb on + strand at 243.308 kb on + strand at 243.308 kb on + strand at 243.309 kb on - strand at 243.309 kb on - strand at 243.309 kb on - strand at 243.309 kb on - strand at 243.309 kb on - strand at 243.309 kb on - strand at 243.314 kb on + strand at 243.315 kb on - strand at 243.315 kb on - strand at 243.349 kb on + strand at 243.349 kb on + strand at 243.350 kb on - strand at 243.350 kb on - strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.369 kb on + strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.370 kb on - strand at 243.371 kb on + strand at 243.371 kb on + strand at 243.371 kb on + strand at 243.371 kb on + strand at 243.371 kb on + strand at 243.371 kb on + strand at 243.371 kb on + strand at 243.372 kb on - strand at 243.372 kb on - strand at 243.372 kb on - strand at 243.372 kb on - strand at 243.372 kb on - strand at 243.372 kb on - strand at 243.372 kb on - strand at 243.511 kb on + strand, within PFLU_RS01100 at 243.512 kb on - strand, within PFLU_RS01100 at 243.539 kb on - strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.551 kb on + strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.552 kb on - strand, within PFLU_RS01100 at 243.556 kb on + strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.557 kb on - strand, within PFLU_RS01100 at 243.574 kb on - strand, within PFLU_RS01100 at 243.685 kb on + strand, within PFLU_RS01100 at 243.781 kb on - strand, within PFLU_RS01100 at 243.781 kb on - strand, within PFLU_RS01100 at 243.792 kb on + strand, within PFLU_RS01100 at 243.838 kb on - strand, within PFLU_RS01100 at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.903 kb on + strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.904 kb on - strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.909 kb on + strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.910 kb on - strand at 243.948 kb on + strand at 243.949 kb on - strand at 243.949 kb on - strand at 244.026 kb on - strand, within PFLU_RS30340 at 244.094 kb on + strand, within PFLU_RS30340 at 244.100 kb on + strand, within PFLU_RS30340 at 244.101 kb on - strand, within PFLU_RS30340 at 244.101 kb on - strand, within PFLU_RS30340 at 244.101 kb on - strand, within PFLU_RS30340 at 244.133 kb on + strand, within PFLU_RS30340 at 244.134 kb on - strand, within PFLU_RS30340 at 244.134 kb on - strand, within PFLU_RS30340 at 244.134 kb on - strand, within PFLU_RS30340 at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.172 kb on + strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.173 kb on - strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.252 kb on + strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.253 kb on - strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.279 kb on + strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.280 kb on - strand at 244.349 kb on + strand at 244.349 kb on + strand at 244.350 kb on - strand at 244.350 kb on - strand at 244.350 kb on - strand at 244.350 kb on - strand at 244.391 kb on - strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.405 kb on + strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.406 kb on - strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.493 kb on + strand at 244.494 kb on - strand at 244.494 kb on - strand at 244.494 kb on - strand at 244.642 kb on + strand, within PFLU_RS01110 at 244.694 kb on - strand, within PFLU_RS01110 at 244.831 kb on - strand, within PFLU_RS01110 at 244.836 kb on - strand, within PFLU_RS01110 at 244.836 kb on - strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.008 kb on + strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.009 kb on - strand, within PFLU_RS01110 at 245.013 kb on + strand, within PFLU_RS01110 at 245.088 kb on - strand at 245.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 242,975 + PFLU_RS01095 0.22 -0.0 242,975 + PFLU_RS01095 0.22 +0.5 242,975 + PFLU_RS01095 0.22 -0.4 243,079 + PFLU_RS01095 0.60 +1.8 243,079 + PFLU_RS01095 0.60 +1.8 243,079 + PFLU_RS01095 0.60 +2.8 243,079 + PFLU_RS01095 0.60 +0.8 243,079 + PFLU_RS01095 0.60 -1.2 243,079 + PFLU_RS01095 0.60 -0.2 243,079 + PFLU_RS01095 0.60 -0.3 243,080 - PFLU_RS01095 0.60 -0.9 243,080 - PFLU_RS01095 0.60 -0.2 243,080 - PFLU_RS01095 0.60 -2.1 243,080 - PFLU_RS01095 0.60 -0.2 243,157 - PFLU_RS01095 0.88 +0.8 243,188 + -0.5 243,188 + -1.2 243,188 + +0.2 243,201 - +0.0 243,201 - +0.1 243,201 - -0.0 243,203 + -2.0 243,256 + -0.8 243,256 + -0.9 243,256 + -0.8 243,257 - +0.2 243,257 - -1.3 243,257 - -0.8 243,257 - +0.5 243,257 - +0.4 243,257 - -0.7 243,257 - +0.6 243,308 + +0.8 243,308 + +0.3 243,308 + -0.3 243,308 + -0.8 243,308 + +0.4 243,308 + -0.3 243,308 + +0.1 243,309 - -0.9 243,309 - -0.5 243,309 - -0.7 243,309 - +1.5 243,309 - +0.1 243,309 - -0.2 243,314 + +0.3 243,315 - -1.8 243,315 - -0.2 243,349 + -0.2 243,349 + -0.7 243,350 - +0.5 243,350 - +1.1 243,369 + -1.3 243,369 + +0.5 243,369 + +0.8 243,369 + +0.6 243,369 + -0.2 243,369 + +0.5 243,369 + -0.2 243,369 + -0.9 243,369 + -0.2 243,369 + +1.5 243,369 + +0.7 243,369 + +0.1 243,369 + -0.5 243,369 + +1.0 243,369 + -0.5 243,369 + +1.2 243,369 + +1.5 243,369 + -0.2 243,369 + -0.2 243,369 + +0.2 243,370 - +0.5 243,370 - +0.5 243,370 - +0.2 243,370 - +0.2 243,370 - -0.9 243,370 - -1.5 243,370 - -0.1 243,370 - +0.1 243,370 - -0.1 243,370 - -0.0 243,370 - +0.2 243,370 - +0.2 243,370 - +0.1 243,370 - -0.2 243,370 - -0.1 243,370 - +0.3 243,370 - +0.2 243,370 - +0.8 243,370 - +0.1 243,370 - +0.3 243,370 - -2.3 243,370 - +1.0 243,371 + -0.1 243,371 + -1.0 243,371 + -0.4 243,371 + +1.1 243,371 + -0.8 243,371 + -0.3 243,371 + +0.1 243,372 - -0.0 243,372 - -0.1 243,372 - -0.8 243,372 - +0.8 243,372 - +0.7 243,372 - -0.4 243,372 - +0.4 243,511 + PFLU_RS01100 0.22 -0.4 243,512 - PFLU_RS01100 0.23 +0.4 243,539 - PFLU_RS01100 0.27 -0.5 243,551 + PFLU_RS01100 0.29 +0.2 243,551 + PFLU_RS01100 0.29 +0.8 243,551 + PFLU_RS01100 0.29 +0.1 243,551 + PFLU_RS01100 0.29 +0.7 243,551 + PFLU_RS01100 0.29 -1.1 243,551 + PFLU_RS01100 0.29 +0.6 243,551 + PFLU_RS01100 0.29 +0.2 243,551 + PFLU_RS01100 0.29 +0.2 243,551 + PFLU_RS01100 0.29 +1.6 243,551 + PFLU_RS01100 0.29 -0.4 243,551 + PFLU_RS01100 0.29 -0.0 243,551 + PFLU_RS01100 0.29 +0.1 243,552 - PFLU_RS01100 0.30 -1.3 243,552 - PFLU_RS01100 0.30 +0.4 243,552 - PFLU_RS01100 0.30 +0.7 243,552 - PFLU_RS01100 0.30 -0.2 243,552 - PFLU_RS01100 0.30 -1.3 243,552 - PFLU_RS01100 0.30 +0.6 243,552 - PFLU_RS01100 0.30 +0.1 243,552 - PFLU_RS01100 0.30 -0.0 243,552 - PFLU_RS01100 0.30 -2.1 243,552 - PFLU_RS01100 0.30 -1.1 243,552 - PFLU_RS01100 0.30 +0.0 243,552 - PFLU_RS01100 0.30 +0.7 243,556 + PFLU_RS01100 0.30 -1.9 243,557 - PFLU_RS01100 0.31 -1.1 243,557 - PFLU_RS01100 0.31 -0.2 243,557 - PFLU_RS01100 0.31 +0.1 243,557 - PFLU_RS01100 0.31 +0.4 243,557 - PFLU_RS01100 0.31 +0.6 243,574 - PFLU_RS01100 0.34 -1.0 243,685 + PFLU_RS01100 0.53 +0.2 243,781 - PFLU_RS01100 0.70 -0.0 243,781 - PFLU_RS01100 0.70 -0.3 243,792 + PFLU_RS01100 0.72 +0.5 243,838 - PFLU_RS01100 0.80 +0.6 243,903 + -0.2 243,903 + -1.5 243,903 + -1.5 243,903 + +0.5 243,903 + +0.4 243,904 - +0.3 243,904 - -0.7 243,904 - -0.5 243,904 - +1.8 243,904 - +1.1 243,904 - -0.4 243,904 - +0.2 243,904 - -0.2 243,909 + +0.2 243,909 + +0.8 243,909 + +0.7 243,909 + +0.2 243,909 + -0.2 243,909 + +0.1 243,909 + -0.0 243,909 + -1.2 243,909 + -0.2 243,909 + +0.7 243,909 + -0.1 243,909 + +1.0 243,909 + +0.1 243,910 - +1.1 243,910 - -0.9 243,910 - -0.3 243,910 - -0.7 243,910 - +0.8 243,910 - +0.3 243,910 - +0.9 243,910 - -1.7 243,910 - -0.5 243,910 - +0.1 243,910 - +0.3 243,948 + +1.0 243,949 - -0.4 243,949 - +0.7 244,026 - PFLU_RS30340 0.40 -1.1 244,094 + PFLU_RS30340 0.68 +0.5 244,100 + PFLU_RS30340 0.71 +0.6 244,101 - PFLU_RS30340 0.71 +0.6 244,101 - PFLU_RS30340 0.71 -0.3 244,101 - PFLU_RS30340 0.71 -0.2 244,133 + PFLU_RS30340 0.85 -0.9 244,134 - PFLU_RS30340 0.85 +0.2 244,134 - PFLU_RS30340 0.85 -1.0 244,134 - PFLU_RS30340 0.85 -0.8 244,172 + +1.0 244,172 + -0.5 244,172 + -0.2 244,172 + +0.3 244,172 + +0.5 244,172 + -0.1 244,172 + -0.2 244,172 + -0.1 244,172 + -0.8 244,172 + +0.1 244,172 + -0.6 244,173 - -1.0 244,173 - +0.2 244,173 - -0.0 244,173 - +0.2 244,173 - -0.1 244,173 - -0.6 244,173 - -0.5 244,173 - +0.1 244,173 - -0.9 244,252 + -0.1 244,252 + +0.6 244,252 + +0.2 244,252 + -0.8 244,252 + -0.4 244,252 + +0.1 244,252 + -0.2 244,252 + -1.1 244,252 + +1.1 244,252 + -0.9 244,252 + +1.2 244,252 + +0.2 244,252 + +1.1 244,252 + +1.1 244,252 + -0.2 244,252 + +1.1 244,252 + -0.6 244,252 + -0.2 244,252 + +0.1 244,252 + -0.5 244,252 + +1.4 244,252 + +1.3 244,252 + -0.7 244,253 - -0.2 244,253 - +0.6 244,253 - +0.3 244,253 - -2.1 244,253 - +0.3 244,253 - -0.4 244,253 - -0.3 244,253 - +0.3 244,279 + +0.1 244,279 + +0.5 244,279 + -1.0 244,279 + -0.2 244,279 + -0.0 244,279 + +0.6 244,279 + -0.6 244,279 + -0.3 244,279 + -0.7 244,280 - -2.4 244,280 - -0.3 244,280 - +0.7 244,280 - -0.8 244,280 - -0.9 244,349 + -0.8 244,349 + +0.2 244,350 - +1.3 244,350 - +1.4 244,350 - -0.7 244,350 - +0.3 244,391 - -0.5 244,405 + +0.4 244,405 + +0.6 244,405 + -0.8 244,405 + +0.3 244,405 + -0.2 244,405 + -1.4 244,405 + +0.6 244,405 + -0.1 244,405 + +1.3 244,405 + -0.4 244,405 + +0.4 244,405 + +1.8 244,405 + +0.5 244,405 + -0.3 244,405 + -0.0 244,405 + +0.1 244,405 + +0.8 244,406 - -1.0 244,406 - +0.6 244,406 - -0.4 244,406 - +0.8 244,406 - +1.8 244,406 - -0.1 244,406 - +0.3 244,406 - +0.2 244,406 - -0.1 244,406 - +1.0 244,406 - -0.9 244,406 - +0.2 244,406 - -0.4 244,406 - +0.3 244,406 - -0.7 244,493 + +0.3 244,493 + +0.2 244,493 + -1.7 244,493 + -0.0 244,493 + +0.1 244,493 + +0.6 244,494 - +0.2 244,494 - +1.2 244,494 - +0.8 244,642 + PFLU_RS01110 0.25 +1.3 244,694 - PFLU_RS01110 0.33 +1.1 244,831 - PFLU_RS01110 0.55 -0.2 244,836 - PFLU_RS01110 0.56 +0.8 244,836 - PFLU_RS01110 0.56 +0.2 245,008 + PFLU_RS01110 0.83 -0.6 245,008 + PFLU_RS01110 0.83 +0.2 245,008 + PFLU_RS01110 0.83 +0.6 245,008 + PFLU_RS01110 0.83 -1.4 245,008 + PFLU_RS01110 0.83 +0.2 245,008 + PFLU_RS01110 0.83 -2.0 245,009 - PFLU_RS01110 0.83 +0.0 245,009 - PFLU_RS01110 0.83 -1.0 245,009 - PFLU_RS01110 0.83 +0.9 245,009 - PFLU_RS01110 0.83 +0.8 245,013 + PFLU_RS01110 0.84 -0.5 245,088 - +0.5 245,088 - -0.6
Or see this region's nucleotide sequence