Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS14070

Experiment: Temperature shift 30_to_4; with MOPS

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Temperature shift 30_to_4; with MOPS
3,150,575	+		PFLU_RS14060	0.74	+1.5
3,150,575	+		PFLU_RS14060	0.74	-0.6
3,150,575	+		PFLU_RS14060	0.74	-0.9
3,150,576	-		PFLU_RS14060	0.75	-0.5
3,150,576	-		PFLU_RS14060	0.75	+0.6
3,150,590	+		PFLU_RS14060	0.79	-0.1
3,150,590	+		PFLU_RS14060	0.79	-0.1
3,150,590	+		PFLU_RS14060	0.79	+1.5
3,150,590	+		PFLU_RS14060	0.79	+1.4
3,150,591	-		PFLU_RS14060	0.79	+0.7
3,150,591	-		PFLU_RS14060	0.79	+0.7
3,150,591	-		PFLU_RS14060	0.79	+0.8
3,150,591	-		PFLU_RS14060	0.79	+1.3
3,150,591	-		PFLU_RS14060	0.79	+1.1
3,150,620	+		PFLU_RS14060	0.88	-0.6
3,150,620	+		PFLU_RS14060	0.88	-0.1
3,150,621	-		PFLU_RS14060	0.88	+0.1
3,150,680	+				+0.5
3,150,680	+				+1.5
3,150,681	-				+0.2
3,150,681	-				+0.9
3,150,681	-				-0.2
3,150,747	+				-0.2
3,150,748	-		PFLU_RS14065	0.10	+0.4
3,150,760	-		PFLU_RS14065	0.12	-0.2
3,150,760	-		PFLU_RS14065	0.12	-0.4
3,150,813	+		PFLU_RS14065	0.19	-0.2
3,150,813	+		PFLU_RS14065	0.19	-0.4
3,150,813	+		PFLU_RS14065	0.19	-0.5
3,150,814	-		PFLU_RS14065	0.19	-0.3
3,150,814	-		PFLU_RS14065	0.19	-0.5
3,150,814	-		PFLU_RS14065	0.19	+2.1
3,150,814	-		PFLU_RS14065	0.19	+1.2
3,150,870	+		PFLU_RS14065	0.26	-0.5
3,150,870	+		PFLU_RS14065	0.26	+1.4
3,150,871	-		PFLU_RS14065	0.26	+0.5
3,150,871	-		PFLU_RS14065	0.26	-0.7
3,151,001	+		PFLU_RS14065	0.44	+0.1
3,151,001	+		PFLU_RS14065	0.44	-1.9
3,151,001	+		PFLU_RS14065	0.44	-1.3
3,151,001	+		PFLU_RS14065	0.44	+0.2
3,151,001	+		PFLU_RS14065	0.44	+0.4
3,151,001	+		PFLU_RS14065	0.44	+0.6
3,151,001	+		PFLU_RS14065	0.44	-0.7
3,151,001	+		PFLU_RS14065	0.44	-0.1
3,151,002	-		PFLU_RS14065	0.44	+0.3
3,151,002	-		PFLU_RS14065	0.44	-0.2
3,151,002	-		PFLU_RS14065	0.44	+1.1
3,151,002	-		PFLU_RS14065	0.44	+0.1
3,151,002	-		PFLU_RS14065	0.44	-0.3
3,151,002	-		PFLU_RS14065	0.44	+2.1
3,151,002	-		PFLU_RS14065	0.44	-0.1
3,151,002	-		PFLU_RS14065	0.44	+0.3
3,151,038	+		PFLU_RS14065	0.49	+0.3
3,151,039	-		PFLU_RS14065	0.49	+1.1
3,151,110	+		PFLU_RS14065	0.58	-0.7
3,151,339	+		PFLU_RS14065	0.89	-0.3
3,151,403	-				+0.3
3,151,526	+		PFLU_RS14070	0.13	+0.7
3,151,526	+		PFLU_RS14070	0.13	+1.5
3,151,526	+		PFLU_RS14070	0.13	-0.0
3,151,526	+		PFLU_RS14070	0.13	+1.3
3,151,589	+		PFLU_RS14070	0.21	-0.5
3,151,590	-		PFLU_RS14070	0.21	-0.4
3,151,590	-		PFLU_RS14070	0.21	+0.1
3,151,826	+		PFLU_RS14070	0.52	+0.4
3,151,827	-		PFLU_RS14070	0.52	-0.7
3,151,827	-		PFLU_RS14070	0.52	+0.1
3,151,919	+		PFLU_RS14070	0.64	+1.1
3,151,944	+		PFLU_RS14070	0.68	+1.8
3,151,944	+		PFLU_RS14070	0.68	-0.0
3,151,975	+		PFLU_RS14070	0.72	-1.5
3,152,019	-		PFLU_RS14070	0.77	+1.7
3,152,019	-		PFLU_RS14070	0.77	+1.3
3,152,019	-		PFLU_RS14070	0.77	+0.3
3,152,019	-		PFLU_RS14070	0.77	-0.3
3,152,096	+		PFLU_RS14070	0.87	-0.1
3,152,116	+				+1.7
3,152,122	-				-0.4
3,152,216	+				+0.1
3,152,216	+				+1.2
3,152,216	+				+0.7
3,152,216	+				+0.4
3,152,216	+				+0.8
3,152,216	+				+0.5
3,152,216	+				-0.5
3,152,216	+				+0.3
3,152,216	+				-0.5
3,152,216	+				-0.7
3,152,216	+				+0.4
3,152,217	-				+0.1
3,152,217	-				+1.7
3,152,217	-				-1.5
3,152,217	-				-0.8
3,152,217	-				-0.5
3,152,217	-				-0.2
3,152,217	-				-1.5
3,152,217	-				-0.5
3,152,217	-				-0.9
3,152,236	-				+0.8
3,152,257	+		PFLU_RS14075	0.16	+0.1
3,152,257	+		PFLU_RS14075	0.16	-0.9
3,152,258	-		PFLU_RS14075	0.17	+0.5
3,152,258	-		PFLU_RS14075	0.17	-1.5
3,152,261	+		PFLU_RS14075	0.18	-0.9
3,152,262	-		PFLU_RS14075	0.18	+0.8
3,152,311	+		PFLU_RS14075	0.38	+0.2
3,152,312	-		PFLU_RS14075	0.39	+0.3
3,152,388	+		PFLU_RS14075	0.70	+1.1
3,152,388	+		PFLU_RS14075	0.70	+1.9
3,152,389	-		PFLU_RS14075	0.70	+1.7
3,152,389	-		PFLU_RS14075	0.70	+0.1
3,152,389	-		PFLU_RS14075	0.70	+0.5
3,152,438	+				-2.3
3,152,460	+				+0.7
3,152,460	+				+1.3
3,152,461	-				-0.6
3,152,461	-				-0.5
3,152,518	+		PFLU_RS30790	0.30	+0.4
3,152,633	+		PFLU_RS30790	0.81	-0.6
3,152,634	-		PFLU_RS30790	0.81	-0.4
3,152,649	+		PFLU_RS30790	0.88	-0.3
3,152,657	+				+0.6
3,152,658	-				-0.5
3,152,680	+				-0.3
3,152,680	+				+0.6
3,152,727	+				-1.1
3,152,728	-				-0.4
3,152,748	+				-1.3
3,152,749	-				+0.2
3,152,749	-				+1.5
3,152,799	+				-1.4
3,152,799	+				-0.3
3,152,799	+				+1.1
3,152,800	-				+2.1
3,152,865	+		PFLU_RS14080	0.11	+1.1
3,152,866	-		PFLU_RS14080	0.11	+0.3
3,152,912	-		PFLU_RS14080	0.15	+1.7
3,152,922	-		PFLU_RS14080	0.16	+0.8
3,152,922	-		PFLU_RS14080	0.16	-0.3
3,152,922	-		PFLU_RS14080	0.16	+0.1
3,152,937	+		PFLU_RS14080	0.18	+0.1
3,152,937	+		PFLU_RS14080	0.18	+0.2
3,152,938	-		PFLU_RS14080	0.18	-1.1
3,152,947	+		PFLU_RS14080	0.18	+0.7
3,152,947	+		PFLU_RS14080	0.18	+0.7
3,152,947	+		PFLU_RS14080	0.18	+3.1
3,152,948	-		PFLU_RS14080	0.19	+0.1
3,152,948	-		PFLU_RS14080	0.19	+0.1
3,152,948	-		PFLU_RS14080	0.19	-0.0
3,152,948	-		PFLU_RS14080	0.19	-2.3
3,152,956	+		PFLU_RS14080	0.19	-0.9
3,152,956	+		PFLU_RS14080	0.19	-1.3
3,152,956	+		PFLU_RS14080	0.19	+0.7
3,152,957	-		PFLU_RS14080	0.19	-0.2
3,152,957	-		PFLU_RS14080	0.19	-1.5
3,152,960	+		PFLU_RS14080	0.20	-0.3
3,152,960	+		PFLU_RS14080	0.20	-0.5
3,152,960	+		PFLU_RS14080	0.20	+0.1
3,152,960	+		PFLU_RS14080	0.20	+1.6
3,152,975	+		PFLU_RS14080	0.21	+1.5
3,152,975	+		PFLU_RS14080	0.21	+0.1
3,152,975	+		PFLU_RS14080	0.21	-2.4
3,152,975	+		PFLU_RS14080	0.21	+0.1
3,152,975	+		PFLU_RS14080	0.21	+0.3
3,152,976	-		PFLU_RS14080	0.21	-0.3
3,152,976	-		PFLU_RS14080	0.21	-0.9
3,152,976	-		PFLU_RS14080	0.21	-0.3
3,152,976	-		PFLU_RS14080	0.21	-1.1
3,152,976	-		PFLU_RS14080	0.21	+0.1
3,152,976	-		PFLU_RS14080	0.21	+0.7
3,152,976	-		PFLU_RS14080	0.21	+0.4
3,152,976	-		PFLU_RS14080	0.21	+1.7
3,153,001	+		PFLU_RS14080	0.23	+1.1
3,153,001	+		PFLU_RS14080	0.23	+0.7
3,153,002	-		PFLU_RS14080	0.24	-0.3
3,153,002	-		PFLU_RS14080	0.24	+1.1
3,153,004	+		PFLU_RS14080	0.24	+0.5
3,153,005	-		PFLU_RS14080	0.24	-0.1
3,153,005	-		PFLU_RS14080	0.24	-0.6
3,153,118	+		PFLU_RS14080	0.34	-0.4
3,153,118	+		PFLU_RS14080	0.34	-1.3