Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02975 and PFLU_RS02980 overlap by 8 nucleotides PFLU_RS02980 and PFLU_RS02985 overlap by 1 nucleotides PFLU_RS02985 and PFLU_RS02990 overlap by 1 nucleotides
PFLU_RS02975: PFLU_RS02975 - precorrin-3B synthase, at 684,611 to 685,957
_RS02975
PFLU_RS02980: PFLU_RS02980 - precorrin-8X methylmutase, at 685,950 to 686,576
_RS02980
PFLU_RS02985: PFLU_RS02985 - precorrin-2 C(20)-methyltransferase, at 686,576 to 687,307
_RS02985
PFLU_RS02990: PFLU_RS02990 - precorrin-3B C(17)-methyltransferase, at 687,307 to 688,944
_RS02990
Position (kb) 686
687
688 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 685.836 kb on - strand at 685.844 kb on + strand at 685.900 kb on + strand at 685.900 kb on + strand at 685.900 kb on + strand at 685.900 kb on + strand at 685.900 kb on + strand at 685.900 kb on + strand at 685.900 kb on + strand at 685.901 kb on - strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.959 kb on + strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.960 kb on - strand at 685.983 kb on + strand at 685.984 kb on - strand at 685.984 kb on - strand at 685.984 kb on - strand at 685.984 kb on - strand at 686.373 kb on + strand, within PFLU_RS02980 at 686.374 kb on - strand, within PFLU_RS02980 at 686.374 kb on - strand, within PFLU_RS02980 at 686.374 kb on - strand, within PFLU_RS02980 at 686.374 kb on - strand, within PFLU_RS02980 at 686.574 kb on + strand at 686.611 kb on - strand at 686.611 kb on - strand at 686.682 kb on - strand, within PFLU_RS02985 at 686.682 kb on - strand, within PFLU_RS02985 at 686.682 kb on - strand, within PFLU_RS02985 at 686.682 kb on - strand, within PFLU_RS02985 at 686.707 kb on + strand, within PFLU_RS02985 at 686.708 kb on - strand, within PFLU_RS02985 at 686.787 kb on + strand, within PFLU_RS02985 at 686.787 kb on + strand, within PFLU_RS02985 at 686.788 kb on - strand, within PFLU_RS02985 at 686.788 kb on - strand, within PFLU_RS02985 at 686.807 kb on + strand, within PFLU_RS02985 at 686.808 kb on - strand, within PFLU_RS02985 at 686.831 kb on + strand, within PFLU_RS02985 at 686.832 kb on - strand, within PFLU_RS02985 at 686.915 kb on + strand, within PFLU_RS02985 at 686.915 kb on + strand, within PFLU_RS02985 at 686.916 kb on - strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.924 kb on + strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.925 kb on - strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.930 kb on + strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.931 kb on - strand, within PFLU_RS02985 at 686.958 kb on - strand, within PFLU_RS02985 at 687.029 kb on + strand, within PFLU_RS02985 at 687.147 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.191 kb on + strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.192 kb on - strand, within PFLU_RS02985 at 687.263 kb on + strand at 687.263 kb on + strand at 687.263 kb on + strand at 687.264 kb on - strand at 687.264 kb on - strand at 687.264 kb on - strand at 687.373 kb on + strand at 687.374 kb on - strand at 687.374 kb on - strand at 687.430 kb on + strand at 687.431 kb on - strand at 687.431 kb on - strand at 687.463 kb on + strand at 687.464 kb on - strand at 687.464 kb on - strand at 687.504 kb on + strand, within PFLU_RS02990 at 687.505 kb on - strand, within PFLU_RS02990 at 687.672 kb on + strand, within PFLU_RS02990 at 687.727 kb on + strand, within PFLU_RS02990 at 687.728 kb on - strand, within PFLU_RS02990 at 687.728 kb on - strand, within PFLU_RS02990 at 687.728 kb on - strand, within PFLU_RS02990 at 687.913 kb on + strand, within PFLU_RS02990 at 687.913 kb on + strand, within PFLU_RS02990 at 687.914 kb on - strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.282 kb on + strand, within PFLU_RS02990 at 688.283 kb on - strand, within PFLU_RS02990
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 685,836 - -1.6 685,844 + -1.5 685,900 + -2.2 685,900 + -0.8 685,900 + -1.6 685,900 + +0.8 685,900 + -0.5 685,900 + -2.1 685,900 + -0.1 685,901 - -0.4 685,959 + +1.0 685,959 + -0.4 685,959 + -0.4 685,959 + -2.3 685,959 + -1.5 685,959 + -1.6 685,959 + -0.5 685,959 + -2.0 685,959 + -0.6 685,959 + -0.1 685,959 + +0.8 685,959 + +1.4 685,960 - +0.1 685,960 - +0.6 685,960 - +0.7 685,960 - -1.0 685,960 - -1.0 685,960 - +0.3 685,960 - +1.2 685,960 - -2.8 685,960 - +0.3 685,960 - +0.3 685,960 - +0.4 685,960 - -0.9 685,983 + -0.9 685,984 - -1.4 685,984 - +1.4 685,984 - -0.9 685,984 - +0.1 686,373 + PFLU_RS02980 0.67 -0.6 686,374 - PFLU_RS02980 0.68 -0.0 686,374 - PFLU_RS02980 0.68 -1.5 686,374 - PFLU_RS02980 0.68 -0.2 686,374 - PFLU_RS02980 0.68 -1.0 686,574 + +0.2 686,611 - -2.8 686,611 - -0.2 686,682 - PFLU_RS02985 0.14 -0.9 686,682 - PFLU_RS02985 0.14 +1.4 686,682 - PFLU_RS02985 0.14 +0.4 686,682 - PFLU_RS02985 0.14 -0.8 686,707 + PFLU_RS02985 0.18 -0.6 686,708 - PFLU_RS02985 0.18 -0.7 686,787 + PFLU_RS02985 0.29 +0.1 686,787 + PFLU_RS02985 0.29 +1.4 686,788 - PFLU_RS02985 0.29 -1.2 686,788 - PFLU_RS02985 0.29 -0.6 686,807 + PFLU_RS02985 0.32 -0.1 686,808 - PFLU_RS02985 0.32 +0.1 686,831 + PFLU_RS02985 0.35 -0.3 686,832 - PFLU_RS02985 0.35 -0.8 686,915 + PFLU_RS02985 0.46 -1.3 686,915 + PFLU_RS02985 0.46 +0.6 686,916 - PFLU_RS02985 0.46 -0.6 686,924 + PFLU_RS02985 0.48 -0.6 686,924 + PFLU_RS02985 0.48 -1.1 686,924 + PFLU_RS02985 0.48 -0.5 686,924 + PFLU_RS02985 0.48 -0.3 686,924 + PFLU_RS02985 0.48 -0.6 686,924 + PFLU_RS02985 0.48 -0.5 686,924 + PFLU_RS02985 0.48 -1.8 686,924 + PFLU_RS02985 0.48 -0.4 686,924 + PFLU_RS02985 0.48 -0.4 686,924 + PFLU_RS02985 0.48 -1.3 686,924 + PFLU_RS02985 0.48 -0.4 686,924 + PFLU_RS02985 0.48 +0.5 686,924 + PFLU_RS02985 0.48 -0.3 686,924 + PFLU_RS02985 0.48 +0.6 686,925 - PFLU_RS02985 0.48 +0.1 686,925 - PFLU_RS02985 0.48 -0.4 686,925 - PFLU_RS02985 0.48 -0.6 686,925 - PFLU_RS02985 0.48 +1.4 686,925 - PFLU_RS02985 0.48 -2.0 686,925 - PFLU_RS02985 0.48 +1.4 686,925 - PFLU_RS02985 0.48 -2.4 686,925 - PFLU_RS02985 0.48 -0.3 686,925 - PFLU_RS02985 0.48 -1.1 686,925 - PFLU_RS02985 0.48 +0.1 686,925 - PFLU_RS02985 0.48 -1.6 686,925 - PFLU_RS02985 0.48 +0.5 686,925 - PFLU_RS02985 0.48 +1.0 686,925 - PFLU_RS02985 0.48 -0.2 686,925 - PFLU_RS02985 0.48 -0.6 686,925 - PFLU_RS02985 0.48 +1.1 686,925 - PFLU_RS02985 0.48 -0.3 686,925 - PFLU_RS02985 0.48 -0.9 686,925 - PFLU_RS02985 0.48 -1.4 686,930 + PFLU_RS02985 0.48 -1.2 686,930 + PFLU_RS02985 0.48 +1.4 686,930 + PFLU_RS02985 0.48 -0.0 686,930 + PFLU_RS02985 0.48 -0.0 686,930 + PFLU_RS02985 0.48 +0.1 686,930 + PFLU_RS02985 0.48 +0.9 686,930 + PFLU_RS02985 0.48 -1.1 686,930 + PFLU_RS02985 0.48 -2.1 686,930 + PFLU_RS02985 0.48 +0.1 686,930 + PFLU_RS02985 0.48 -1.2 686,930 + PFLU_RS02985 0.48 -1.5 686,930 + PFLU_RS02985 0.48 -0.3 686,930 + PFLU_RS02985 0.48 -0.9 686,930 + PFLU_RS02985 0.48 -1.9 686,930 + PFLU_RS02985 0.48 -1.2 686,930 + PFLU_RS02985 0.48 -2.6 686,930 + PFLU_RS02985 0.48 -0.3 686,930 + PFLU_RS02985 0.48 -1.3 686,930 + PFLU_RS02985 0.48 +0.6 686,931 - PFLU_RS02985 0.48 +1.4 686,931 - PFLU_RS02985 0.48 +0.1 686,931 - PFLU_RS02985 0.48 -0.8 686,931 - PFLU_RS02985 0.48 +0.2 686,931 - PFLU_RS02985 0.48 -0.5 686,931 - PFLU_RS02985 0.48 -1.8 686,931 - PFLU_RS02985 0.48 -1.1 686,931 - PFLU_RS02985 0.48 -1.1 686,931 - PFLU_RS02985 0.48 -0.5 686,931 - PFLU_RS02985 0.48 -0.8 686,931 - PFLU_RS02985 0.48 -0.5 686,931 - PFLU_RS02985 0.48 +0.4 686,931 - PFLU_RS02985 0.48 -0.1 686,931 - PFLU_RS02985 0.48 -1.0 686,931 - PFLU_RS02985 0.48 +0.5 686,931 - PFLU_RS02985 0.48 +0.3 686,958 - PFLU_RS02985 0.52 -1.5 687,029 + PFLU_RS02985 0.62 -0.3 687,147 + PFLU_RS02985 0.78 +0.7 687,191 + PFLU_RS02985 0.84 -1.6 687,191 + PFLU_RS02985 0.84 -1.8 687,191 + PFLU_RS02985 0.84 -0.9 687,191 + PFLU_RS02985 0.84 -1.3 687,191 + PFLU_RS02985 0.84 -0.6 687,191 + PFLU_RS02985 0.84 -0.4 687,191 + PFLU_RS02985 0.84 -1.6 687,191 + PFLU_RS02985 0.84 -1.4 687,192 - PFLU_RS02985 0.84 -0.1 687,192 - PFLU_RS02985 0.84 +0.5 687,192 - PFLU_RS02985 0.84 +0.7 687,192 - PFLU_RS02985 0.84 +0.2 687,192 - PFLU_RS02985 0.84 -0.3 687,192 - PFLU_RS02985 0.84 +0.2 687,192 - PFLU_RS02985 0.84 -0.1 687,263 + -0.8 687,263 + -1.1 687,263 + +0.0 687,264 - -0.6 687,264 - +0.2 687,264 - -0.4 687,373 + -0.6 687,374 - -0.1 687,374 - -0.0 687,430 + +0.4 687,431 - -1.7 687,431 - -1.3 687,463 + -0.3 687,464 - -0.7 687,464 - -0.1 687,504 + PFLU_RS02990 0.12 -0.7 687,505 - PFLU_RS02990 0.12 -1.5 687,672 + PFLU_RS02990 0.22 +1.4 687,727 + PFLU_RS02990 0.26 -0.9 687,728 - PFLU_RS02990 0.26 -0.8 687,728 - PFLU_RS02990 0.26 -0.5 687,728 - PFLU_RS02990 0.26 -1.0 687,913 + PFLU_RS02990 0.37 -1.3 687,913 + PFLU_RS02990 0.37 +0.4 687,914 - PFLU_RS02990 0.37 -2.0 688,282 + PFLU_RS02990 0.60 -0.6 688,282 + PFLU_RS02990 0.60 +0.4 688,282 + PFLU_RS02990 0.60 +0.4 688,282 + PFLU_RS02990 0.60 -1.2 688,282 + PFLU_RS02990 0.60 +0.7 688,282 + PFLU_RS02990 0.60 -1.1 688,283 - PFLU_RS02990 0.60 -0.5
Or see this region's nucleotide sequence