Experiment: Temperature shift 30_to_4; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS01165 and PFLU_RS01170 are separated by 40 nucleotides PFLU_RS01170 and PFLU_RS01175 overlap by 11 nucleotides PFLU_RS01175 and PFLU_RS01180 overlap by 4 nucleotides PFLU_RS01180 and PFLU_RS01185 are separated by 81 nucleotides
PFLU_RS01165: PFLU_RS01165 - class I SAM-dependent methyltransferase, at 258,809 to 259,435
_RS01165
PFLU_RS01170: PFLU_RS01170 - ABC transporter permease, at 259,476 to 260,120
_RS01170
PFLU_RS01175: PFLU_RS01175 - ATP-binding cassette domain-containing protein, at 260,110 to 261,225
_RS01175
PFLU_RS01180: PFLU_RS01180 - methionine ABC transporter substrate-binding protein, at 261,222 to 262,037
_RS01180
PFLU_RS01185: PFLU_RS01185 - LLM class flavin-dependent oxidoreductase, at 262,119 to 263,471
_RS01185
Position (kb) 260
261
262 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 259.209 kb on - strand, within PFLU_RS01165 at 259.209 kb on - strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.300 kb on + strand, within PFLU_RS01165 at 259.380 kb on + strand at 259.380 kb on + strand at 259.380 kb on + strand at 259.380 kb on + strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.381 kb on - strand at 259.426 kb on + strand at 259.427 kb on - strand at 259.427 kb on - strand at 259.470 kb on + strand at 259.470 kb on + strand at 259.471 kb on - strand at 259.471 kb on - strand at 259.471 kb on - strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.472 kb on + strand at 259.473 kb on - strand at 259.473 kb on - strand at 259.574 kb on + strand, within PFLU_RS01170 at 259.588 kb on + strand, within PFLU_RS01170 at 259.600 kb on + strand, within PFLU_RS01170 at 259.863 kb on + strand, within PFLU_RS01170 at 259.870 kb on + strand, within PFLU_RS01170 at 259.870 kb on + strand, within PFLU_RS01170 at 259.870 kb on + strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.871 kb on - strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.874 kb on + strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.875 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 259.991 kb on - strand, within PFLU_RS01170 at 260.103 kb on + strand at 260.104 kb on - strand at 260.104 kb on - strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.120 kb on + strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.121 kb on - strand at 260.338 kb on + strand, within PFLU_RS01175 at 260.338 kb on + strand, within PFLU_RS01175 at 260.338 kb on + strand, within PFLU_RS01175 at 260.339 kb on - strand, within PFLU_RS01175 at 260.339 kb on - strand, within PFLU_RS01175 at 260.339 kb on - strand, within PFLU_RS01175 at 260.377 kb on + strand, within PFLU_RS01175 at 260.378 kb on - strand, within PFLU_RS01175 at 260.378 kb on - strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.973 kb on + strand, within PFLU_RS01175 at 260.974 kb on - strand, within PFLU_RS01175 at 260.974 kb on - strand, within PFLU_RS01175 at 260.974 kb on - strand, within PFLU_RS01175 at 261.104 kb on + strand, within PFLU_RS01175 at 261.104 kb on + strand, within PFLU_RS01175 at 261.104 kb on + strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.105 kb on - strand, within PFLU_RS01175 at 261.123 kb on + strand at 261.124 kb on - strand at 261.231 kb on - strand at 261.250 kb on + strand at 261.250 kb on + strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.251 kb on - strand at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.304 kb on + strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.305 kb on - strand, within PFLU_RS01180 at 261.373 kb on + strand, within PFLU_RS01180 at 261.373 kb on + strand, within PFLU_RS01180 at 261.373 kb on + strand, within PFLU_RS01180 at 261.374 kb on - strand, within PFLU_RS01180 at 261.441 kb on - strand, within PFLU_RS01180 at 261.442 kb on + strand, within PFLU_RS01180 at 261.442 kb on + strand, within PFLU_RS01180 at 261.442 kb on + strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.443 kb on - strand, within PFLU_RS01180 at 261.478 kb on + strand, within PFLU_RS01180 at 261.479 kb on - strand, within PFLU_RS01180 at 261.562 kb on + strand, within PFLU_RS01180 at 261.562 kb on + strand, within PFLU_RS01180 at 261.562 kb on + strand, within PFLU_RS01180 at 261.563 kb on - strand, within PFLU_RS01180 at 261.563 kb on - strand, within PFLU_RS01180 at 261.563 kb on - strand, within PFLU_RS01180 at 261.567 kb on + strand, within PFLU_RS01180 at 261.567 kb on + strand, within PFLU_RS01180 at 261.567 kb on + strand, within PFLU_RS01180 at 261.568 kb on - strand, within PFLU_RS01180 at 261.568 kb on - strand, within PFLU_RS01180 at 261.568 kb on - strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.688 kb on + strand, within PFLU_RS01180 at 261.689 kb on - strand, within PFLU_RS01180 at 261.689 kb on - strand, within PFLU_RS01180 at 261.689 kb on - strand, within PFLU_RS01180 at 261.700 kb on + strand, within PFLU_RS01180 at 261.701 kb on - strand, within PFLU_RS01180 at 261.701 kb on - strand, within PFLU_RS01180 at 261.796 kb on + strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 261.797 kb on - strand, within PFLU_RS01180 at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.054 kb on + strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.055 kb on - strand at 262.205 kb on - strand at 262.216 kb on + strand at 262.216 kb on + strand at 262.216 kb on + strand at 262.216 kb on + strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand at 262.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 30_to_4; with MOPS remove 259,209 - PFLU_RS01165 0.64 -0.7 259,209 - PFLU_RS01165 0.64 +0.1 259,300 + PFLU_RS01165 0.78 -1.2 259,300 + PFLU_RS01165 0.78 -0.8 259,300 + PFLU_RS01165 0.78 -0.4 259,300 + PFLU_RS01165 0.78 -1.5 259,380 + +0.8 259,380 + -2.2 259,380 + -0.8 259,380 + +0.1 259,381 - +1.4 259,381 - +0.0 259,381 - +0.1 259,381 - +1.1 259,381 - +0.6 259,426 + +1.0 259,427 - -0.8 259,427 - +1.3 259,470 + +0.4 259,470 + -1.0 259,471 - +0.4 259,471 - -0.5 259,471 - +1.0 259,472 + -0.5 259,472 + +0.4 259,472 + +0.8 259,472 + +0.5 259,472 + +1.4 259,472 + +0.8 259,472 + +0.0 259,473 - -0.0 259,473 - +0.0 259,574 + PFLU_RS01170 0.15 -1.2 259,588 + PFLU_RS01170 0.17 -1.4 259,600 + PFLU_RS01170 0.19 +0.2 259,863 + PFLU_RS01170 0.60 -0.4 259,870 + PFLU_RS01170 0.61 -0.4 259,870 + PFLU_RS01170 0.61 +0.6 259,870 + PFLU_RS01170 0.61 -1.2 259,871 - PFLU_RS01170 0.61 -0.1 259,871 - PFLU_RS01170 0.61 -0.5 259,871 - PFLU_RS01170 0.61 +0.4 259,871 - PFLU_RS01170 0.61 -0.3 259,874 + PFLU_RS01170 0.62 +1.0 259,874 + PFLU_RS01170 0.62 -0.5 259,874 + PFLU_RS01170 0.62 -0.5 259,874 + PFLU_RS01170 0.62 +0.8 259,874 + PFLU_RS01170 0.62 +0.7 259,875 - PFLU_RS01170 0.62 -0.8 259,875 - PFLU_RS01170 0.62 +0.3 259,875 - PFLU_RS01170 0.62 +0.8 259,875 - PFLU_RS01170 0.62 +0.7 259,875 - PFLU_RS01170 0.62 -0.5 259,875 - PFLU_RS01170 0.62 -0.7 259,875 - PFLU_RS01170 0.62 +0.2 259,991 - PFLU_RS01170 0.80 +1.0 259,991 - PFLU_RS01170 0.80 +0.5 259,991 - PFLU_RS01170 0.80 -2.6 259,991 - PFLU_RS01170 0.80 -0.4 259,991 - PFLU_RS01170 0.80 -0.1 260,103 + -0.6 260,104 - +1.3 260,104 - -0.5 260,120 + -0.3 260,120 + +0.2 260,120 + +1.6 260,120 + -0.6 260,121 - +2.8 260,121 - -0.0 260,121 - -0.4 260,121 - +0.0 260,121 - -0.3 260,121 - +0.1 260,121 - +1.1 260,121 - +0.1 260,338 + PFLU_RS01175 0.20 -0.4 260,338 + PFLU_RS01175 0.20 -0.3 260,338 + PFLU_RS01175 0.20 -2.5 260,339 - PFLU_RS01175 0.21 -1.7 260,339 - PFLU_RS01175 0.21 +2.1 260,339 - PFLU_RS01175 0.21 -0.9 260,377 + PFLU_RS01175 0.24 -0.3 260,378 - PFLU_RS01175 0.24 -0.3 260,378 - PFLU_RS01175 0.24 -0.2 260,973 + PFLU_RS01175 0.77 +1.3 260,973 + PFLU_RS01175 0.77 +0.0 260,973 + PFLU_RS01175 0.77 -0.0 260,973 + PFLU_RS01175 0.77 +0.1 260,973 + PFLU_RS01175 0.77 -0.5 260,973 + PFLU_RS01175 0.77 +0.7 260,973 + PFLU_RS01175 0.77 -0.7 260,974 - PFLU_RS01175 0.77 +0.5 260,974 - PFLU_RS01175 0.77 +0.4 260,974 - PFLU_RS01175 0.77 -1.2 261,104 + PFLU_RS01175 0.89 -1.0 261,104 + PFLU_RS01175 0.89 +0.6 261,104 + PFLU_RS01175 0.89 -1.0 261,105 - PFLU_RS01175 0.89 +0.3 261,105 - PFLU_RS01175 0.89 -0.1 261,105 - PFLU_RS01175 0.89 +0.1 261,105 - PFLU_RS01175 0.89 -1.1 261,105 - PFLU_RS01175 0.89 +1.1 261,105 - PFLU_RS01175 0.89 +0.1 261,105 - PFLU_RS01175 0.89 -0.4 261,123 + +0.5 261,124 - +0.0 261,231 - +0.5 261,250 + -0.5 261,250 + -1.7 261,251 - -0.5 261,251 - -0.9 261,251 - -0.1 261,251 - -0.6 261,251 - +0.6 261,304 + PFLU_RS01180 0.10 +1.0 261,304 + PFLU_RS01180 0.10 -1.8 261,304 + PFLU_RS01180 0.10 -0.4 261,304 + PFLU_RS01180 0.10 -1.2 261,304 + PFLU_RS01180 0.10 +0.4 261,304 + PFLU_RS01180 0.10 +0.7 261,304 + PFLU_RS01180 0.10 -0.6 261,304 + PFLU_RS01180 0.10 -0.2 261,305 - PFLU_RS01180 0.10 +0.3 261,305 - PFLU_RS01180 0.10 +0.4 261,305 - PFLU_RS01180 0.10 -0.1 261,305 - PFLU_RS01180 0.10 -0.1 261,305 - PFLU_RS01180 0.10 -0.1 261,373 + PFLU_RS01180 0.19 -1.4 261,373 + PFLU_RS01180 0.19 -1.5 261,373 + PFLU_RS01180 0.19 -1.3 261,374 - PFLU_RS01180 0.19 -0.9 261,441 - PFLU_RS01180 0.27 +0.3 261,442 + PFLU_RS01180 0.27 -0.2 261,442 + PFLU_RS01180 0.27 -0.1 261,442 + PFLU_RS01180 0.27 -0.1 261,443 - PFLU_RS01180 0.27 +0.7 261,443 - PFLU_RS01180 0.27 -0.1 261,443 - PFLU_RS01180 0.27 +0.3 261,443 - PFLU_RS01180 0.27 -1.2 261,443 - PFLU_RS01180 0.27 -0.7 261,443 - PFLU_RS01180 0.27 -2.3 261,443 - PFLU_RS01180 0.27 -0.5 261,478 + PFLU_RS01180 0.31 +0.8 261,479 - PFLU_RS01180 0.31 -0.5 261,562 + PFLU_RS01180 0.42 +1.2 261,562 + PFLU_RS01180 0.42 -0.6 261,562 + PFLU_RS01180 0.42 +0.5 261,563 - PFLU_RS01180 0.42 -0.5 261,563 - PFLU_RS01180 0.42 +0.5 261,563 - PFLU_RS01180 0.42 -2.0 261,567 + PFLU_RS01180 0.42 -0.5 261,567 + PFLU_RS01180 0.42 -0.2 261,567 + PFLU_RS01180 0.42 -0.5 261,568 - PFLU_RS01180 0.42 -0.4 261,568 - PFLU_RS01180 0.42 -0.1 261,568 - PFLU_RS01180 0.42 -0.1 261,688 + PFLU_RS01180 0.57 +0.6 261,688 + PFLU_RS01180 0.57 -0.2 261,688 + PFLU_RS01180 0.57 -0.2 261,688 + PFLU_RS01180 0.57 +0.2 261,688 + PFLU_RS01180 0.57 +0.3 261,688 + PFLU_RS01180 0.57 -0.5 261,688 + PFLU_RS01180 0.57 +1.4 261,689 - PFLU_RS01180 0.57 +0.2 261,689 - PFLU_RS01180 0.57 -0.2 261,689 - PFLU_RS01180 0.57 +0.2 261,700 + PFLU_RS01180 0.59 +0.1 261,701 - PFLU_RS01180 0.59 -0.1 261,701 - PFLU_RS01180 0.59 +0.4 261,796 + PFLU_RS01180 0.70 +0.3 261,797 - PFLU_RS01180 0.70 -0.8 261,797 - PFLU_RS01180 0.70 -0.2 261,797 - PFLU_RS01180 0.70 -0.2 261,797 - PFLU_RS01180 0.70 +1.8 261,797 - PFLU_RS01180 0.70 +1.4 261,797 - PFLU_RS01180 0.70 +0.9 262,054 + -1.0 262,054 + -1.6 262,054 + +1.2 262,054 + -0.5 262,054 + +0.5 262,054 + -0.9 262,054 + -1.8 262,054 + +0.1 262,055 - +1.1 262,055 - +1.0 262,055 - +1.1 262,055 - +1.1 262,055 - -0.8 262,055 - +0.6 262,055 - -1.0 262,055 - +1.9 262,055 - +0.2 262,055 - -1.2 262,205 - +1.1 262,216 + +0.2 262,216 + +0.3 262,216 + -0.2 262,216 + -0.3 262,217 - -0.3 262,217 - +0.5 262,217 - -0.8 262,217 - -0.1 262,217 - -2.0 262,217 - +1.0 262,217 - +0.2 262,217 - +0.3
Or see this region's nucleotide sequence