Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_0541 and Ga0059261_0542 are separated by 86 nucleotides Ga0059261_0542 and Ga0059261_0543 overlap by 4 nucleotides
Ga0059261_0541: Ga0059261_0541 - Outer membrane receptor proteins, mostly Fe transport, at 547,003 to 549,501
_0541
Ga0059261_0542: Ga0059261_0542 - type I secretion membrane fusion protein, HlyD family, at 549,588 to 550,799
_0542
Ga0059261_0543: Ga0059261_0543 - type I secretion system ATPase, LssB family, at 550,796 to 552,961
_0543
Position (kb) 549
550
551 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 548.599 kb on + strand, within Ga0059261_0541 at 548.600 kb on - strand, within Ga0059261_0541 at 548.600 kb on - strand, within Ga0059261_0541 at 548.600 kb on - strand, within Ga0059261_0541 at 548.600 kb on - strand, within Ga0059261_0541 at 548.600 kb on - strand, within Ga0059261_0541 at 548.616 kb on + strand, within Ga0059261_0541 at 548.616 kb on + strand, within Ga0059261_0541 at 548.616 kb on + strand, within Ga0059261_0541 at 548.617 kb on - strand at 548.726 kb on - strand, within Ga0059261_0541 at 548.770 kb on + strand, within Ga0059261_0541 at 548.770 kb on + strand, within Ga0059261_0541 at 548.770 kb on + strand, within Ga0059261_0541 at 548.771 kb on - strand, within Ga0059261_0541 at 548.771 kb on - strand, within Ga0059261_0541 at 548.833 kb on + strand, within Ga0059261_0541 at 548.833 kb on + strand, within Ga0059261_0541 at 548.833 kb on + strand, within Ga0059261_0541 at 548.834 kb on - strand, within Ga0059261_0541 at 548.851 kb on + strand, within Ga0059261_0541 at 548.851 kb on + strand, within Ga0059261_0541 at 548.852 kb on - strand, within Ga0059261_0541 at 548.857 kb on + strand, within Ga0059261_0541 at 548.857 kb on + strand, within Ga0059261_0541 at 548.858 kb on - strand, within Ga0059261_0541 at 548.858 kb on - strand, within Ga0059261_0541 at 548.858 kb on - strand, within Ga0059261_0541 at 548.858 kb on - strand, within Ga0059261_0541 at 548.858 kb on - strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.914 kb on + strand, within Ga0059261_0541 at 548.915 kb on - strand, within Ga0059261_0541 at 548.915 kb on - strand at 548.915 kb on - strand, within Ga0059261_0541 at 548.915 kb on - strand, within Ga0059261_0541 at 548.915 kb on - strand, within Ga0059261_0541 at 548.915 kb on - strand, within Ga0059261_0541 at 548.916 kb on + strand, within Ga0059261_0541 at 548.916 kb on + strand, within Ga0059261_0541 at 548.916 kb on + strand, within Ga0059261_0541 at 548.916 kb on + strand, within Ga0059261_0541 at 548.916 kb on + strand, within Ga0059261_0541 at 548.917 kb on - strand, within Ga0059261_0541 at 548.917 kb on - strand, within Ga0059261_0541 at 548.917 kb on - strand, within Ga0059261_0541 at 548.997 kb on + strand, within Ga0059261_0541 at 548.997 kb on + strand, within Ga0059261_0541 at 548.997 kb on + strand, within Ga0059261_0541 at 548.997 kb on + strand, within Ga0059261_0541 at 548.997 kb on + strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 548.998 kb on - strand, within Ga0059261_0541 at 549.001 kb on + strand, within Ga0059261_0541 at 549.001 kb on + strand, within Ga0059261_0541 at 549.001 kb on + strand, within Ga0059261_0541 at 549.001 kb on + strand at 549.001 kb on + strand, within Ga0059261_0541 at 549.001 kb on + strand, within Ga0059261_0541 at 549.002 kb on - strand, within Ga0059261_0541 at 549.002 kb on - strand, within Ga0059261_0541 at 549.002 kb on - strand, within Ga0059261_0541 at 549.066 kb on + strand, within Ga0059261_0541 at 549.067 kb on - strand, within Ga0059261_0541 at 549.196 kb on + strand, within Ga0059261_0541 at 549.196 kb on + strand, within Ga0059261_0541 at 549.196 kb on + strand, within Ga0059261_0541 at 549.226 kb on + strand, within Ga0059261_0541 at 549.226 kb on + strand, within Ga0059261_0541 at 549.227 kb on - strand, within Ga0059261_0541 at 549.227 kb on - strand, within Ga0059261_0541 at 549.227 kb on - strand, within Ga0059261_0541 at 549.247 kb on + strand, within Ga0059261_0541 at 549.314 kb on - strand at 549.314 kb on - strand at 549.314 kb on - strand at 549.314 kb on - strand at 549.314 kb on - strand at 549.314 kb on - strand at 549.340 kb on + strand at 549.341 kb on - strand at 549.355 kb on + strand at 549.355 kb on + strand at 549.355 kb on + strand at 549.355 kb on + strand at 549.357 kb on + strand at 549.357 kb on + strand at 549.357 kb on + strand at 549.357 kb on + strand at 549.357 kb on + strand at 549.358 kb on - strand at 549.358 kb on - strand at 549.358 kb on - strand at 549.358 kb on - strand at 549.381 kb on + strand at 549.382 kb on - strand at 549.382 kb on - strand at 549.382 kb on - strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.445 kb on + strand at 549.446 kb on - strand at 549.446 kb on - strand at 549.447 kb on + strand at 549.447 kb on + strand at 549.448 kb on - strand at 549.468 kb on + strand at 549.468 kb on + strand at 549.468 kb on + strand at 549.468 kb on + strand at 549.468 kb on + strand at 549.469 kb on - strand at 549.469 kb on - strand at 549.500 kb on - strand at 549.500 kb on - strand at 549.500 kb on - strand at 549.500 kb on - strand at 549.590 kb on - strand at 549.590 kb on - strand at 549.590 kb on - strand at 549.590 kb on - strand at 549.637 kb on + strand at 549.637 kb on + strand at 549.638 kb on - strand at 549.638 kb on - strand at 549.645 kb on + strand at 549.646 kb on - strand at 549.646 kb on - strand at 549.646 kb on - strand at 549.722 kb on + strand, within Ga0059261_0542 at 549.723 kb on - strand, within Ga0059261_0542 at 549.761 kb on - strand, within Ga0059261_0542 at 549.763 kb on + strand, within Ga0059261_0542 at 549.763 kb on + strand, within Ga0059261_0542 at 549.763 kb on + strand, within Ga0059261_0542 at 549.763 kb on + strand, within Ga0059261_0542 at 549.763 kb on + strand, within Ga0059261_0542 at 549.764 kb on - strand, within Ga0059261_0542 at 549.764 kb on - strand, within Ga0059261_0542 at 549.764 kb on - strand, within Ga0059261_0542 at 549.797 kb on + strand, within Ga0059261_0542 at 549.838 kb on + strand, within Ga0059261_0542 at 549.838 kb on + strand, within Ga0059261_0542 at 549.853 kb on + strand at 549.853 kb on + strand, within Ga0059261_0542 at 549.854 kb on - strand, within Ga0059261_0542 at 549.854 kb on - strand, within Ga0059261_0542 at 549.854 kb on - strand, within Ga0059261_0542 at 549.854 kb on - strand, within Ga0059261_0542 at 550.120 kb on + strand, within Ga0059261_0542 at 550.120 kb on + strand, within Ga0059261_0542 at 550.121 kb on - strand, within Ga0059261_0542 at 550.121 kb on - strand, within Ga0059261_0542 at 550.397 kb on + strand, within Ga0059261_0542 at 550.398 kb on - strand, within Ga0059261_0542 at 550.398 kb on - strand, within Ga0059261_0542 at 550.398 kb on - strand, within Ga0059261_0542 at 550.398 kb on - strand, within Ga0059261_0542 at 550.607 kb on + strand, within Ga0059261_0542 at 550.608 kb on - strand, within Ga0059261_0542 at 550.644 kb on - strand, within Ga0059261_0542 at 550.652 kb on - strand, within Ga0059261_0542 at 550.652 kb on - strand at 550.652 kb on - strand at 550.738 kb on + strand at 550.738 kb on + strand at 550.738 kb on + strand at 550.738 kb on + strand at 550.739 kb on - strand at 550.739 kb on - strand at 550.984 kb on + strand at 551.056 kb on + strand, within Ga0059261_0543 at 551.057 kb on - strand, within Ga0059261_0543 at 551.057 kb on - strand, within Ga0059261_0543 at 551.292 kb on + strand, within Ga0059261_0543 at 551.293 kb on - strand, within Ga0059261_0543 at 551.293 kb on - strand, within Ga0059261_0543 at 551.293 kb on - strand, within Ga0059261_0543 at 551.293 kb on - strand, within Ga0059261_0543 at 551.502 kb on + strand, within Ga0059261_0543 at 551.502 kb on + strand, within Ga0059261_0543 at 551.503 kb on - strand, within Ga0059261_0543 at 551.503 kb on - strand, within Ga0059261_0543 at 551.503 kb on - strand, within Ga0059261_0543 at 551.529 kb on + strand, within Ga0059261_0543 at 551.529 kb on + strand, within Ga0059261_0543 at 551.529 kb on + strand, within Ga0059261_0543 at 551.530 kb on - strand, within Ga0059261_0543 at 551.530 kb on - strand, within Ga0059261_0543 at 551.530 kb on - strand, within Ga0059261_0543 at 551.530 kb on - strand, within Ga0059261_0543 at 551.530 kb on - strand, within Ga0059261_0543 at 551.770 kb on + strand at 551.770 kb on + strand, within Ga0059261_0543 at 551.770 kb on + strand, within Ga0059261_0543 at 551.770 kb on + strand, within Ga0059261_0543 at 551.770 kb on + strand, within Ga0059261_0543 at 551.770 kb on + strand, within Ga0059261_0543 at 551.770 kb on + strand, within Ga0059261_0543 at 551.771 kb on - strand, within Ga0059261_0543 at 551.771 kb on - strand, within Ga0059261_0543 at 551.772 kb on + strand, within Ga0059261_0543 at 551.772 kb on + strand at 551.773 kb on - strand, within Ga0059261_0543 at 551.773 kb on - strand, within Ga0059261_0543 at 551.773 kb on - strand, within Ga0059261_0543
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 548,599 + Ga0059261_0541 0.64 +0.9 548,600 - Ga0059261_0541 0.64 +0.3 548,600 - Ga0059261_0541 0.64 -1.0 548,600 - Ga0059261_0541 0.64 +0.2 548,600 - Ga0059261_0541 0.64 +0.1 548,600 - Ga0059261_0541 0.64 +0.7 548,616 + Ga0059261_0541 0.65 +0.2 548,616 + Ga0059261_0541 0.65 +2.2 548,616 + Ga0059261_0541 0.65 +0.7 548,617 - +1.0 548,726 - Ga0059261_0541 0.69 +2.8 548,770 + Ga0059261_0541 0.71 +0.9 548,770 + Ga0059261_0541 0.71 -0.5 548,770 + Ga0059261_0541 0.71 -0.3 548,771 - Ga0059261_0541 0.71 -1.4 548,771 - Ga0059261_0541 0.71 +1.8 548,833 + Ga0059261_0541 0.73 -0.6 548,833 + Ga0059261_0541 0.73 -0.3 548,833 + Ga0059261_0541 0.73 +1.0 548,834 - Ga0059261_0541 0.73 +1.4 548,851 + Ga0059261_0541 0.74 -1.1 548,851 + Ga0059261_0541 0.74 +0.1 548,852 - Ga0059261_0541 0.74 -0.6 548,857 + Ga0059261_0541 0.74 -0.6 548,857 + Ga0059261_0541 0.74 -1.7 548,858 - Ga0059261_0541 0.74 -1.8 548,858 - Ga0059261_0541 0.74 -0.2 548,858 - Ga0059261_0541 0.74 -1.1 548,858 - Ga0059261_0541 0.74 -1.7 548,858 - Ga0059261_0541 0.74 -0.9 548,914 + Ga0059261_0541 0.76 -0.9 548,914 + Ga0059261_0541 0.76 +0.7 548,914 + Ga0059261_0541 0.76 -1.1 548,914 + Ga0059261_0541 0.76 +0.2 548,914 + Ga0059261_0541 0.76 +1.0 548,914 + Ga0059261_0541 0.76 -0.3 548,914 + Ga0059261_0541 0.76 +0.8 548,915 - Ga0059261_0541 0.77 -0.9 548,915 - -0.3 548,915 - Ga0059261_0541 0.77 +0.9 548,915 - Ga0059261_0541 0.77 -0.5 548,915 - Ga0059261_0541 0.77 -1.7 548,915 - Ga0059261_0541 0.77 -0.7 548,916 + Ga0059261_0541 0.77 +0.2 548,916 + Ga0059261_0541 0.77 -1.1 548,916 + Ga0059261_0541 0.77 -0.4 548,916 + Ga0059261_0541 0.77 +0.1 548,916 + Ga0059261_0541 0.77 +1.2 548,917 - Ga0059261_0541 0.77 -0.8 548,917 - Ga0059261_0541 0.77 -1.6 548,917 - Ga0059261_0541 0.77 -0.8 548,997 + Ga0059261_0541 0.80 +2.0 548,997 + Ga0059261_0541 0.80 -0.9 548,997 + Ga0059261_0541 0.80 +0.0 548,997 + Ga0059261_0541 0.80 +0.2 548,997 + Ga0059261_0541 0.80 +0.0 548,998 - Ga0059261_0541 0.80 -0.6 548,998 - Ga0059261_0541 0.80 +3.0 548,998 - Ga0059261_0541 0.80 -0.6 548,998 - Ga0059261_0541 0.80 +1.2 548,998 - Ga0059261_0541 0.80 -1.7 548,998 - Ga0059261_0541 0.80 +0.7 548,998 - Ga0059261_0541 0.80 -0.1 548,998 - Ga0059261_0541 0.80 -0.6 548,998 - Ga0059261_0541 0.80 +1.1 548,998 - Ga0059261_0541 0.80 +2.4 549,001 + Ga0059261_0541 0.80 +0.5 549,001 + Ga0059261_0541 0.80 +0.3 549,001 + Ga0059261_0541 0.80 -0.2 549,001 + +1.0 549,001 + Ga0059261_0541 0.80 -0.2 549,001 + Ga0059261_0541 0.80 -0.7 549,002 - Ga0059261_0541 0.80 +1.4 549,002 - Ga0059261_0541 0.80 +0.0 549,002 - Ga0059261_0541 0.80 -0.4 549,066 + Ga0059261_0541 0.83 -2.0 549,067 - Ga0059261_0541 0.83 +0.1 549,196 + Ga0059261_0541 0.88 +1.8 549,196 + Ga0059261_0541 0.88 +1.3 549,196 + Ga0059261_0541 0.88 -1.7 549,226 + Ga0059261_0541 0.89 -0.1 549,226 + Ga0059261_0541 0.89 +0.4 549,227 - Ga0059261_0541 0.89 -1.3 549,227 - Ga0059261_0541 0.89 +1.9 549,227 - Ga0059261_0541 0.89 +0.5 549,247 + Ga0059261_0541 0.90 -0.2 549,314 - -2.6 549,314 - -0.2 549,314 - -1.5 549,314 - +1.2 549,314 - +0.2 549,314 - -1.7 549,340 + -1.0 549,341 - +2.6 549,355 + +0.0 549,355 + +0.3 549,355 + -1.5 549,355 + -0.5 549,357 + +3.7 549,357 + +0.3 549,357 + +1.0 549,357 + -1.1 549,357 + -0.2 549,358 - +1.2 549,358 - +1.4 549,358 - +3.0 549,358 - -3.1 549,381 + -1.9 549,382 - -1.8 549,382 - +0.0 549,382 - +0.1 549,445 + -1.4 549,445 + -0.0 549,445 + +3.8 549,445 + +0.8 549,445 + -1.1 549,445 + -1.0 549,445 + +0.4 549,445 + -2.1 549,445 + -0.3 549,445 + +3.2 549,445 + -0.3 549,446 - +1.3 549,446 - +0.2 549,447 + +1.6 549,447 + +2.0 549,448 - +2.5 549,468 + -0.4 549,468 + +2.0 549,468 + -1.2 549,468 + -1.7 549,468 + +2.4 549,469 - +2.2 549,469 - +1.5 549,500 - -3.0 549,500 - +0.7 549,500 - +3.5 549,500 - -0.8 549,590 - +2.8 549,590 - -0.7 549,590 - -0.2 549,590 - +0.5 549,637 + +3.4 549,637 + +0.6 549,638 - -1.5 549,638 - -0.5 549,645 + +1.7 549,646 - +0.3 549,646 - +1.0 549,646 - -1.2 549,722 + Ga0059261_0542 0.11 +1.9 549,723 - Ga0059261_0542 0.11 +0.0 549,761 - Ga0059261_0542 0.14 -1.2 549,763 + Ga0059261_0542 0.14 -0.2 549,763 + Ga0059261_0542 0.14 +1.8 549,763 + Ga0059261_0542 0.14 -0.0 549,763 + Ga0059261_0542 0.14 +1.3 549,763 + Ga0059261_0542 0.14 -2.4 549,764 - Ga0059261_0542 0.15 -1.1 549,764 - Ga0059261_0542 0.15 -1.1 549,764 - Ga0059261_0542 0.15 -0.5 549,797 + Ga0059261_0542 0.17 +0.2 549,838 + Ga0059261_0542 0.21 +0.4 549,838 + Ga0059261_0542 0.21 -0.7 549,853 + +2.0 549,853 + Ga0059261_0542 0.22 -0.3 549,854 - Ga0059261_0542 0.22 +0.2 549,854 - Ga0059261_0542 0.22 -0.7 549,854 - Ga0059261_0542 0.22 +3.6 549,854 - Ga0059261_0542 0.22 -0.5 550,120 + Ga0059261_0542 0.44 +0.2 550,120 + Ga0059261_0542 0.44 -1.7 550,121 - Ga0059261_0542 0.44 -1.1 550,121 - Ga0059261_0542 0.44 +3.2 550,397 + Ga0059261_0542 0.67 -1.1 550,398 - Ga0059261_0542 0.67 +0.0 550,398 - Ga0059261_0542 0.67 -1.0 550,398 - Ga0059261_0542 0.67 -0.6 550,398 - Ga0059261_0542 0.67 +0.7 550,607 + Ga0059261_0542 0.84 +0.5 550,608 - Ga0059261_0542 0.84 -0.6 550,644 - Ga0059261_0542 0.87 +0.5 550,652 - Ga0059261_0542 0.88 +3.0 550,652 - -0.8 550,652 - +1.0 550,738 + -0.3 550,738 + +1.7 550,738 + -1.0 550,738 + -0.6 550,739 - +1.6 550,739 - -1.0 550,984 + +0.1 551,056 + Ga0059261_0543 0.12 -0.5 551,057 - Ga0059261_0543 0.12 -0.2 551,057 - Ga0059261_0543 0.12 -1.9 551,292 + Ga0059261_0543 0.23 -1.0 551,293 - Ga0059261_0543 0.23 -1.4 551,293 - Ga0059261_0543 0.23 +0.1 551,293 - Ga0059261_0543 0.23 +0.6 551,293 - Ga0059261_0543 0.23 +2.0 551,502 + Ga0059261_0543 0.33 -0.3 551,502 + Ga0059261_0543 0.33 -1.2 551,503 - Ga0059261_0543 0.33 +0.5 551,503 - Ga0059261_0543 0.33 -0.7 551,503 - Ga0059261_0543 0.33 +1.5 551,529 + Ga0059261_0543 0.34 -0.3 551,529 + Ga0059261_0543 0.34 +1.0 551,529 + Ga0059261_0543 0.34 +0.6 551,530 - Ga0059261_0543 0.34 -1.9 551,530 - Ga0059261_0543 0.34 +0.1 551,530 - Ga0059261_0543 0.34 -0.5 551,530 - Ga0059261_0543 0.34 -1.1 551,530 - Ga0059261_0543 0.34 -0.6 551,770 + +4.5 551,770 + Ga0059261_0543 0.45 -1.2 551,770 + Ga0059261_0543 0.45 +0.9 551,770 + Ga0059261_0543 0.45 +1.2 551,770 + Ga0059261_0543 0.45 +0.5 551,770 + Ga0059261_0543 0.45 -1.3 551,770 + Ga0059261_0543 0.45 +2.6 551,771 - Ga0059261_0543 0.45 -0.7 551,771 - Ga0059261_0543 0.45 +0.7 551,772 + Ga0059261_0543 0.45 -0.8 551,772 + +1.5 551,773 - Ga0059261_0543 0.45 +0.6 551,773 - Ga0059261_0543 0.45 +0.8 551,773 - Ga0059261_0543 0.45 +0.5
Or see this region's nucleotide sequence