Experiment: inner cut, LB soft agar motility assay
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_0106 and Ga0059261_0107 are separated by 100 nucleotides Ga0059261_0107 and Ga0059261_0108 are separated by 107 nucleotides Ga0059261_0108 and Ga0059261_0109 are separated by 38 nucleotides Ga0059261_0109 and Ga0059261_0110 are separated by 104 nucleotides
Ga0059261_0106: Ga0059261_0106 - Outer membrane receptor proteins, mostly Fe transport, at 101,060 to 103,765
_0106
Ga0059261_0107: Ga0059261_0107 - 2OG-Fe(II) oxygenase superfamily, at 103,866 to 104,510
_0107
Ga0059261_0108: Ga0059261_0108 - hypothetical protein, at 104,618 to 105,661
_0108
Ga0059261_0109: Ga0059261_0109 - tRNA, at 105,700 to 105,776
_0109
Ga0059261_0110: Ga0059261_0110 - Glutamate dehydrogenase/leucine dehydrogenase, at 105,881 to 106,933
_0110
Position (kb) 104
105
106 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 103.667 kb on + strand at 103.667 kb on + strand at 103.667 kb on + strand at 103.676 kb on + strand at 103.676 kb on + strand at 103.677 kb on - strand at 103.706 kb on + strand at 103.706 kb on + strand at 103.707 kb on - strand at 103.707 kb on - strand at 103.707 kb on - strand at 103.784 kb on + strand at 103.867 kb on + strand at 103.867 kb on + strand at 103.867 kb on + strand at 103.868 kb on - strand at 103.868 kb on - strand at 103.868 kb on - strand at 103.906 kb on + strand at 103.906 kb on + strand at 103.906 kb on + strand at 103.906 kb on + strand at 103.906 kb on + strand at 103.906 kb on + strand at 103.907 kb on - strand at 103.907 kb on - strand at 103.907 kb on - strand at 103.907 kb on - strand at 103.907 kb on - strand at 104.071 kb on + strand, within Ga0059261_0107 at 104.071 kb on + strand, within Ga0059261_0107 at 104.071 kb on + strand, within Ga0059261_0107 at 104.071 kb on + strand, within Ga0059261_0107 at 104.071 kb on + strand, within Ga0059261_0107 at 104.072 kb on - strand, within Ga0059261_0107 at 104.072 kb on - strand, within Ga0059261_0107 at 104.072 kb on - strand, within Ga0059261_0107 at 104.147 kb on - strand, within Ga0059261_0107 at 104.147 kb on - strand, within Ga0059261_0107 at 104.153 kb on + strand, within Ga0059261_0107 at 104.153 kb on + strand at 104.182 kb on + strand, within Ga0059261_0107 at 104.183 kb on - strand, within Ga0059261_0107 at 104.183 kb on - strand, within Ga0059261_0107 at 104.321 kb on + strand, within Ga0059261_0107 at 104.322 kb on - strand, within Ga0059261_0107 at 104.322 kb on - strand, within Ga0059261_0107 at 104.322 kb on - strand, within Ga0059261_0107 at 104.322 kb on - strand, within Ga0059261_0107 at 104.322 kb on - strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.323 kb on + strand, within Ga0059261_0107 at 104.324 kb on - strand, within Ga0059261_0107 at 104.324 kb on - strand, within Ga0059261_0107 at 104.324 kb on - strand, within Ga0059261_0107 at 104.492 kb on - strand at 104.518 kb on - strand at 104.518 kb on - strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.554 kb on + strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.555 kb on - strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.556 kb on + strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.557 kb on - strand at 104.574 kb on + strand at 104.574 kb on + strand at 104.575 kb on - strand at 104.606 kb on + strand at 104.606 kb on + strand at 104.606 kb on + strand at 104.606 kb on + strand at 104.606 kb on + strand at 104.606 kb on + strand at 104.606 kb on + strand at 104.607 kb on - strand at 104.812 kb on + strand, within Ga0059261_0108 at 104.812 kb on + strand, within Ga0059261_0108 at 104.812 kb on + strand, within Ga0059261_0108 at 104.813 kb on - strand, within Ga0059261_0108 at 104.813 kb on - strand, within Ga0059261_0108 at 104.813 kb on - strand, within Ga0059261_0108 at 104.813 kb on - strand, within Ga0059261_0108 at 104.813 kb on - strand, within Ga0059261_0108 at 104.813 kb on - strand, within Ga0059261_0108 at 105.005 kb on + strand at 105.005 kb on + strand at 105.005 kb on + strand, within Ga0059261_0108 at 105.006 kb on - strand, within Ga0059261_0108 at 105.006 kb on - strand, within Ga0059261_0108 at 105.006 kb on - strand, within Ga0059261_0108 at 105.006 kb on - strand, within Ga0059261_0108 at 105.351 kb on - strand, within Ga0059261_0108 at 105.351 kb on - strand, within Ga0059261_0108 at 105.389 kb on + strand, within Ga0059261_0108 at 105.389 kb on + strand, within Ga0059261_0108 at 105.410 kb on + strand, within Ga0059261_0108 at 105.410 kb on + strand, within Ga0059261_0108 at 105.410 kb on + strand, within Ga0059261_0108 at 105.410 kb on + strand, within Ga0059261_0108 at 105.411 kb on - strand, within Ga0059261_0108 at 105.411 kb on - strand, within Ga0059261_0108 at 105.411 kb on - strand, within Ga0059261_0108 at 105.411 kb on - strand, within Ga0059261_0108 at 105.526 kb on + strand, within Ga0059261_0108 at 105.622 kb on + strand at 105.623 kb on - strand at 105.643 kb on + strand at 105.790 kb on + strand at 105.790 kb on + strand at 105.790 kb on + strand at 105.790 kb on + strand at 105.791 kb on - strand at 105.791 kb on - strand at 105.792 kb on + strand at 105.792 kb on + strand at 105.792 kb on + strand at 105.792 kb on + strand at 105.792 kb on + strand at 105.793 kb on - strand at 105.793 kb on - strand at 105.793 kb on - strand at 105.829 kb on + strand at 105.837 kb on + strand at 105.837 kb on + strand at 105.838 kb on - strand at 105.880 kb on + strand at 105.880 kb on + strand at 105.880 kb on + strand at 105.880 kb on + strand at 105.880 kb on + strand at 105.881 kb on - strand at 105.881 kb on - strand at 105.881 kb on - strand at 105.881 kb on - strand at 105.881 kb on - strand at 105.881 kb on - strand at 105.881 kb on - strand at 105.881 kb on - strand at 106.025 kb on + strand, within Ga0059261_0110 at 106.025 kb on + strand, within Ga0059261_0110 at 106.025 kb on + strand, within Ga0059261_0110 at 106.026 kb on - strand, within Ga0059261_0110 at 106.214 kb on + strand, within Ga0059261_0110 at 106.214 kb on + strand, within Ga0059261_0110 at 106.214 kb on + strand, within Ga0059261_0110 at 106.214 kb on + strand, within Ga0059261_0110 at 106.214 kb on + strand, within Ga0059261_0110 at 106.215 kb on - strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.352 kb on + strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand, within Ga0059261_0110 at 106.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction inner cut, LB soft agar motility assay remove 103,667 + +0.7 103,667 + -0.8 103,667 + -0.9 103,676 + -1.3 103,676 + +2.7 103,677 - -0.0 103,706 + +0.1 103,706 + -0.7 103,707 - -0.6 103,707 - +2.3 103,707 - -0.7 103,784 + -1.8 103,867 + +0.0 103,867 + -0.7 103,867 + +1.1 103,868 - -2.5 103,868 - -0.2 103,868 - +0.9 103,906 + +1.6 103,906 + -0.6 103,906 + +0.5 103,906 + -1.8 103,906 + -1.0 103,906 + -0.2 103,907 - -0.0 103,907 - +1.0 103,907 - -0.6 103,907 - +1.1 103,907 - -1.0 104,071 + Ga0059261_0107 0.32 +0.4 104,071 + Ga0059261_0107 0.32 -2.0 104,071 + Ga0059261_0107 0.32 -0.8 104,071 + Ga0059261_0107 0.32 +0.7 104,071 + Ga0059261_0107 0.32 -0.4 104,072 - Ga0059261_0107 0.32 -0.7 104,072 - Ga0059261_0107 0.32 +2.2 104,072 - Ga0059261_0107 0.32 -0.2 104,147 - Ga0059261_0107 0.44 +0.1 104,147 - Ga0059261_0107 0.44 +0.1 104,153 + Ga0059261_0107 0.44 +0.8 104,153 + +0.5 104,182 + Ga0059261_0107 0.49 -0.4 104,183 - Ga0059261_0107 0.49 -3.3 104,183 - Ga0059261_0107 0.49 +2.1 104,321 + Ga0059261_0107 0.71 -1.9 104,322 - Ga0059261_0107 0.71 +2.1 104,322 - Ga0059261_0107 0.71 +0.7 104,322 - Ga0059261_0107 0.71 +5.3 104,322 - Ga0059261_0107 0.71 +1.5 104,322 - Ga0059261_0107 0.71 +1.5 104,323 + Ga0059261_0107 0.71 +0.1 104,323 + Ga0059261_0107 0.71 -1.3 104,323 + Ga0059261_0107 0.71 +0.3 104,323 + Ga0059261_0107 0.71 -0.0 104,323 + Ga0059261_0107 0.71 +0.7 104,323 + Ga0059261_0107 0.71 -1.3 104,323 + Ga0059261_0107 0.71 -0.8 104,323 + Ga0059261_0107 0.71 -0.2 104,323 + Ga0059261_0107 0.71 -0.7 104,324 - Ga0059261_0107 0.71 -1.9 104,324 - Ga0059261_0107 0.71 -0.5 104,324 - Ga0059261_0107 0.71 -0.9 104,492 - -0.8 104,518 - -2.4 104,518 - +0.1 104,554 + +0.8 104,554 + +0.1 104,554 + -1.7 104,554 + -0.2 104,554 + -0.7 104,554 + -0.4 104,554 + +3.8 104,554 + +1.7 104,554 + +1.4 104,554 + +0.0 104,555 - +1.1 104,555 - -1.4 104,555 - +0.2 104,555 - -1.3 104,555 - -0.3 104,555 - -1.4 104,555 - -0.3 104,555 - +0.1 104,555 - -0.8 104,556 + +0.5 104,556 + +0.9 104,556 + +0.2 104,556 + -0.2 104,556 + -0.5 104,556 + +1.8 104,556 + -0.9 104,556 + +0.7 104,556 + -1.1 104,556 + +0.1 104,556 + -1.2 104,557 - -1.6 104,557 - +0.1 104,557 - +1.3 104,557 - -1.2 104,557 - +1.2 104,557 - -0.0 104,557 - -1.8 104,557 - +1.7 104,557 - -0.2 104,557 - -0.7 104,574 + +3.5 104,574 + -0.9 104,575 - +0.1 104,606 + +0.8 104,606 + +1.1 104,606 + +0.3 104,606 + +2.1 104,606 + -0.2 104,606 + +0.6 104,606 + -0.6 104,607 - +3.5 104,812 + Ga0059261_0108 0.19 +0.9 104,812 + Ga0059261_0108 0.19 +0.8 104,812 + Ga0059261_0108 0.19 -1.6 104,813 - Ga0059261_0108 0.19 +1.1 104,813 - Ga0059261_0108 0.19 +0.5 104,813 - Ga0059261_0108 0.19 -2.3 104,813 - Ga0059261_0108 0.19 -0.3 104,813 - Ga0059261_0108 0.19 -1.3 104,813 - Ga0059261_0108 0.19 +0.9 105,005 + +2.1 105,005 + +0.5 105,005 + Ga0059261_0108 0.37 -0.5 105,006 - Ga0059261_0108 0.37 -2.1 105,006 - Ga0059261_0108 0.37 -1.3 105,006 - Ga0059261_0108 0.37 +0.4 105,006 - Ga0059261_0108 0.37 -0.3 105,351 - Ga0059261_0108 0.70 +0.2 105,351 - Ga0059261_0108 0.70 +1.2 105,389 + Ga0059261_0108 0.74 +0.3 105,389 + Ga0059261_0108 0.74 +1.8 105,410 + Ga0059261_0108 0.76 +0.8 105,410 + Ga0059261_0108 0.76 +0.8 105,410 + Ga0059261_0108 0.76 +1.3 105,410 + Ga0059261_0108 0.76 -1.5 105,411 - Ga0059261_0108 0.76 +1.6 105,411 - Ga0059261_0108 0.76 -1.6 105,411 - Ga0059261_0108 0.76 +3.6 105,411 - Ga0059261_0108 0.76 +0.3 105,526 + Ga0059261_0108 0.87 +0.4 105,622 + -0.3 105,623 - -0.2 105,643 + +1.1 105,790 + -2.0 105,790 + -0.3 105,790 + +0.1 105,790 + +0.2 105,791 - +0.3 105,791 - -0.6 105,792 + -0.6 105,792 + -0.6 105,792 + -1.3 105,792 + +0.3 105,792 + -1.0 105,793 - -0.2 105,793 - -1.7 105,793 - -1.2 105,829 + +2.5 105,837 + +0.3 105,837 + +0.4 105,838 - +0.1 105,880 + +0.4 105,880 + -3.8 105,880 + +1.1 105,880 + +0.1 105,880 + +0.1 105,881 - -0.5 105,881 - +0.5 105,881 - +0.3 105,881 - +0.4 105,881 - -0.6 105,881 - +1.4 105,881 - +1.4 105,881 - -1.7 106,025 + Ga0059261_0110 0.14 +2.7 106,025 + Ga0059261_0110 0.14 -1.0 106,025 + Ga0059261_0110 0.14 +1.1 106,026 - Ga0059261_0110 0.14 +0.2 106,214 + Ga0059261_0110 0.32 +0.1 106,214 + Ga0059261_0110 0.32 +0.3 106,214 + Ga0059261_0110 0.32 -0.9 106,214 + Ga0059261_0110 0.32 -2.9 106,214 + Ga0059261_0110 0.32 +0.3 106,215 - Ga0059261_0110 0.32 +0.0 106,352 + Ga0059261_0110 0.45 -0.3 106,352 + Ga0059261_0110 0.45 +1.5 106,352 + Ga0059261_0110 0.45 +0.8 106,352 + Ga0059261_0110 0.45 +1.1 106,352 + Ga0059261_0110 0.45 +0.8 106,352 + Ga0059261_0110 0.45 -0.3 106,352 + Ga0059261_0110 0.45 -1.0 106,352 + Ga0059261_0110 0.45 -0.3 106,353 - Ga0059261_0110 0.45 -1.0 106,353 - Ga0059261_0110 0.45 -2.3 106,353 - Ga0059261_0110 0.45 -1.3 106,353 - Ga0059261_0110 0.45 +3.8 106,353 - Ga0059261_0110 0.45 +1.7 106,353 - Ga0059261_0110 0.45 -1.1 106,353 - Ga0059261_0110 0.45 +2.4 106,353 - Ga0059261_0110 0.45 -0.8 106,353 - +0.5
Or see this region's nucleotide sequence