Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22985 and AO356_22990 are separated by 1 nucleotides AO356_22990 and AO356_22995 are separated by 192 nucleotides AO356_22995 and AO356_23000 are separated by 127 nucleotides
AO356_22985: AO356_22985 - branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit (EC 1.2.4.4) (from data) , at 5,131,547 to 5,132,605
_22985
AO356_22990: AO356_22990 - branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit (EC 1.2.4.4) (from data) , at 5,132,607 to 5,133,842
_22990
AO356_22995: AO356_22995 - ArsR family transcriptional regulator, at 5,134,035 to 5,134,523
_22995
AO356_23000: AO356_23000 - MBL fold metallo-hydrolase, at 5,134,651 to 5,135,514
_23000
Position (kb)
5132
5133
5134 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5131.869 kb on + strand, within AO356_22985 at 5131.869 kb on + strand, within AO356_22985 at 5131.870 kb on - strand, within AO356_22985 at 5131.870 kb on - strand, within AO356_22985 at 5131.884 kb on + strand, within AO356_22985 at 5131.947 kb on + strand, within AO356_22985 at 5131.947 kb on + strand, within AO356_22985 at 5131.947 kb on + strand, within AO356_22985 at 5131.947 kb on + strand, within AO356_22985 at 5131.947 kb on + strand, within AO356_22985 at 5131.948 kb on - strand, within AO356_22985 at 5131.948 kb on - strand, within AO356_22985 at 5131.948 kb on - strand, within AO356_22985 at 5131.948 kb on - strand, within AO356_22985 at 5131.948 kb on - strand, within AO356_22985 at 5132.029 kb on + strand, within AO356_22985 at 5132.031 kb on + strand, within AO356_22985 at 5132.032 kb on - strand, within AO356_22985 at 5132.190 kb on + strand, within AO356_22985 at 5132.304 kb on + strand, within AO356_22985 at 5132.304 kb on + strand, within AO356_22985 at 5132.305 kb on - strand, within AO356_22985 at 5132.305 kb on - strand, within AO356_22985 at 5132.418 kb on + strand, within AO356_22985 at 5132.418 kb on + strand, within AO356_22985 at 5132.419 kb on - strand, within AO356_22985 at 5132.584 kb on + strand at 5132.584 kb on + strand at 5132.585 kb on - strand at 5132.660 kb on - strand at 5132.660 kb on - strand at 5132.879 kb on - strand, within AO356_22990 at 5132.992 kb on + strand, within AO356_22990 at 5133.331 kb on + strand, within AO356_22990 at 5133.331 kb on + strand, within AO356_22990 at 5133.331 kb on + strand, within AO356_22990 at 5133.331 kb on + strand, within AO356_22990 at 5133.332 kb on - strand, within AO356_22990 at 5133.442 kb on + strand, within AO356_22990 at 5133.442 kb on + strand, within AO356_22990 at 5133.523 kb on + strand, within AO356_22990 at 5133.523 kb on + strand, within AO356_22990 at 5133.523 kb on + strand, within AO356_22990 at 5133.524 kb on - strand, within AO356_22990 at 5133.571 kb on + strand, within AO356_22990 at 5133.571 kb on + strand, within AO356_22990 at 5133.572 kb on - strand, within AO356_22990 at 5133.572 kb on - strand, within AO356_22990 at 5133.764 kb on - strand at 5133.764 kb on - strand at 5133.829 kb on + strand at 5133.830 kb on - strand at 5133.830 kb on - strand at 5133.851 kb on - strand at 5133.924 kb on - strand at 5133.934 kb on + strand at 5133.935 kb on - strand at 5133.966 kb on + strand at 5134.025 kb on + strand at 5134.330 kb on - strand, within AO356_22995 at 5134.354 kb on - strand, within AO356_22995 at 5134.473 kb on + strand, within AO356_22995 at 5134.473 kb on + strand, within AO356_22995 at 5134.473 kb on + strand, within AO356_22995 at 5134.589 kb on + strand at 5134.642 kb on + strand at 5134.643 kb on - strand at 5134.652 kb on + strand at 5134.652 kb on + strand at 5134.652 kb on + strand at 5134.653 kb on - strand at 5134.785 kb on + strand, within AO356_23000
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 5,131,869 + AO356_22985 0.30 +0.3 5,131,869 + AO356_22985 0.30 +0.3 5,131,870 - AO356_22985 0.31 -0.7 5,131,870 - AO356_22985 0.31 -1.6 5,131,884 + AO356_22985 0.32 +0.0 5,131,947 + AO356_22985 0.38 -2.7 5,131,947 + AO356_22985 0.38 -1.9 5,131,947 + AO356_22985 0.38 -2.2 5,131,947 + AO356_22985 0.38 -1.4 5,131,947 + AO356_22985 0.38 -1.3 5,131,948 - AO356_22985 0.38 -0.3 5,131,948 - AO356_22985 0.38 -1.4 5,131,948 - AO356_22985 0.38 -1.9 5,131,948 - AO356_22985 0.38 -0.5 5,131,948 - AO356_22985 0.38 -0.7 5,132,029 + AO356_22985 0.46 -2.7 5,132,031 + AO356_22985 0.46 +0.7 5,132,032 - AO356_22985 0.46 -0.9 5,132,190 + AO356_22985 0.61 -0.6 5,132,304 + AO356_22985 0.71 -1.2 5,132,304 + AO356_22985 0.71 -1.6 5,132,305 - AO356_22985 0.72 -2.8 5,132,305 - AO356_22985 0.72 +0.3 5,132,418 + AO356_22985 0.82 -1.4 5,132,418 + AO356_22985 0.82 -1.2 5,132,419 - AO356_22985 0.82 -0.5 5,132,584 + -2.2 5,132,584 + -1.2 5,132,585 - -1.0 5,132,660 - -0.7 5,132,660 - -0.6 5,132,879 - AO356_22990 0.22 +0.3 5,132,992 + AO356_22990 0.31 -0.4 5,133,331 + AO356_22990 0.59 -0.5 5,133,331 + AO356_22990 0.59 -2.7 5,133,331 + AO356_22990 0.59 +0.4 5,133,331 + AO356_22990 0.59 -1.9 5,133,332 - AO356_22990 0.59 +0.0 5,133,442 + AO356_22990 0.68 -1.7 5,133,442 + AO356_22990 0.68 -0.6 5,133,523 + AO356_22990 0.74 -2.0 5,133,523 + AO356_22990 0.74 -1.0 5,133,523 + AO356_22990 0.74 -1.7 5,133,524 - AO356_22990 0.74 -1.7 5,133,571 + AO356_22990 0.78 -1.3 5,133,571 + AO356_22990 0.78 -1.3 5,133,572 - AO356_22990 0.78 -1.3 5,133,572 - AO356_22990 0.78 -1.7 5,133,764 - -0.0 5,133,764 - -1.1 5,133,829 + -1.5 5,133,830 - -1.6 5,133,830 - -0.6 5,133,851 - -0.9 5,133,924 - -0.5 5,133,934 + -1.3 5,133,935 - -0.2 5,133,966 + -2.6 5,134,025 + -0.3 5,134,330 - AO356_22995 0.60 -1.2 5,134,354 - AO356_22995 0.65 -1.9 5,134,473 + AO356_22995 0.90 -0.3 5,134,473 + AO356_22995 0.90 -1.4 5,134,473 + AO356_22995 0.90 -1.5 5,134,589 + +0.1 5,134,642 + +1.3 5,134,643 - +1.1 5,134,652 + +0.0 5,134,652 + +1.3 5,134,652 + +0.3 5,134,653 - +1.1 5,134,785 + AO356_23000 0.16 -0.7
Or see this region's nucleotide sequence