Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_09795 and AO356_09800 are separated by 9 nucleotides AO356_09800 and AO356_09805 are separated by 42 nucleotides AO356_09805 and AO356_09810 overlap by 1 nucleotides AO356_09810 and AO356_09815 overlap by 1 nucleotides
AO356_09795: AO356_09795 - imidazole glycerol phosphate synthase subunit HisF, at 2,220,811 to 2,221,581
_09795
AO356_09800: AO356_09800 - 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) , at 2,221,591 to 2,222,328
_09800
AO356_09805: AO356_09805 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase, at 2,222,371 to 2,222,625
_09805
AO356_09810: AO356_09810 - imidazole glycerol phosphate synthase subunit HisH, at 2,222,625 to 2,223,263
_09810
AO356_09815: AO356_09815 - imidazoleglycerol-phosphate dehydratase, at 2,223,263 to 2,223,856
_09815
Position (kb)
2222
2223 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2221.386 kb on + strand, within AO356_09795 at 2221.387 kb on - strand, within AO356_09795 at 2221.602 kb on - strand at 2221.649 kb on + strand at 2221.649 kb on + strand at 2221.833 kb on - strand, within AO356_09800 at 2221.888 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.030 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.051 kb on + strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.052 kb on - strand, within AO356_09800 at 2222.284 kb on + strand at 2222.284 kb on + strand at 2222.284 kb on + strand at 2222.284 kb on + strand at 2222.285 kb on - strand at 2222.345 kb on + strand at 2222.345 kb on + strand at 2222.346 kb on - strand at 2222.346 kb on - strand at 2222.346 kb on - strand at 2222.351 kb on + strand at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.460 kb on - strand, within AO356_09805 at 2222.626 kb on + strand at 2222.626 kb on + strand at 2222.627 kb on - strand at 2222.678 kb on + strand at 2222.797 kb on + strand, within AO356_09810 at 2222.797 kb on + strand, within AO356_09810 at 2222.797 kb on + strand, within AO356_09810 at 2222.797 kb on + strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.798 kb on - strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.800 kb on + strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.801 kb on - strand, within AO356_09810 at 2222.816 kb on - strand, within AO356_09810 at 2222.816 kb on - strand, within AO356_09810 at 2223.264 kb on + strand at 2223.489 kb on + strand, within AO356_09815 at 2223.490 kb on - strand, within AO356_09815 at 2223.490 kb on - strand, within AO356_09815 at 2223.490 kb on - strand, within AO356_09815 at 2223.567 kb on + strand, within AO356_09815 at 2223.568 kb on - strand, within AO356_09815 at 2223.568 kb on - strand, within AO356_09815 at 2223.568 kb on - strand, within AO356_09815 at 2223.568 kb on - strand, within AO356_09815 at 2223.568 kb on - strand, within AO356_09815 at 2223.616 kb on + strand, within AO356_09815
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 2,221,386 + AO356_09795 0.75 -1.8 2,221,387 - AO356_09795 0.75 -2.8 2,221,602 - +0.1 2,221,649 + -2.0 2,221,649 + -1.2 2,221,833 - AO356_09800 0.33 -2.0 2,221,888 + AO356_09800 0.40 -2.9 2,222,030 + AO356_09800 0.59 -2.0 2,222,030 + AO356_09800 0.59 -2.1 2,222,030 + AO356_09800 0.59 -1.7 2,222,030 + AO356_09800 0.59 -3.0 2,222,030 + AO356_09800 0.59 -2.5 2,222,051 + AO356_09800 0.62 -0.9 2,222,051 + AO356_09800 0.62 -2.5 2,222,051 + AO356_09800 0.62 -2.0 2,222,051 + AO356_09800 0.62 +0.1 2,222,051 + AO356_09800 0.62 -1.3 2,222,052 - AO356_09800 0.62 +0.1 2,222,052 - AO356_09800 0.62 -2.1 2,222,052 - AO356_09800 0.62 -1.2 2,222,052 - AO356_09800 0.62 -1.8 2,222,284 + -2.0 2,222,284 + -1.9 2,222,284 + -2.6 2,222,284 + -2.5 2,222,285 - -1.2 2,222,345 + -1.6 2,222,345 + -1.7 2,222,346 - -0.3 2,222,346 - +0.4 2,222,346 - +0.3 2,222,351 + -1.7 2,222,460 - AO356_09805 0.35 +0.0 2,222,460 - AO356_09805 0.35 +0.8 2,222,460 - AO356_09805 0.35 -1.5 2,222,460 - AO356_09805 0.35 +0.0 2,222,460 - AO356_09805 0.35 +0.5 2,222,626 + -2.9 2,222,626 + -1.7 2,222,627 - -0.2 2,222,678 + -1.3 2,222,797 + AO356_09810 0.27 -2.5 2,222,797 + AO356_09810 0.27 -2.1 2,222,797 + AO356_09810 0.27 -2.2 2,222,797 + AO356_09810 0.27 -1.9 2,222,798 - AO356_09810 0.27 -3.0 2,222,798 - AO356_09810 0.27 -1.2 2,222,798 - AO356_09810 0.27 -1.5 2,222,798 - AO356_09810 0.27 -2.0 2,222,798 - AO356_09810 0.27 -1.5 2,222,800 + AO356_09810 0.27 -2.3 2,222,800 + AO356_09810 0.27 -2.0 2,222,800 + AO356_09810 0.27 -2.0 2,222,800 + AO356_09810 0.27 -2.4 2,222,800 + AO356_09810 0.27 -1.8 2,222,800 + AO356_09810 0.27 -1.5 2,222,800 + AO356_09810 0.27 -1.9 2,222,801 - AO356_09810 0.28 -3.0 2,222,801 - AO356_09810 0.28 -3.0 2,222,801 - AO356_09810 0.28 -2.4 2,222,801 - AO356_09810 0.28 -0.9 2,222,816 - AO356_09810 0.30 -2.6 2,222,816 - AO356_09810 0.30 -0.2 2,223,264 + -2.6 2,223,489 + AO356_09815 0.38 -0.9 2,223,490 - AO356_09815 0.38 -3.0 2,223,490 - AO356_09815 0.38 -2.5 2,223,490 - AO356_09815 0.38 -1.3 2,223,567 + AO356_09815 0.51 -2.5 2,223,568 - AO356_09815 0.51 -3.5 2,223,568 - AO356_09815 0.51 -3.0 2,223,568 - AO356_09815 0.51 -3.2 2,223,568 - AO356_09815 0.51 -3.5 2,223,568 - AO356_09815 0.51 -1.0 2,223,616 + AO356_09815 0.59 -0.9
Or see this region's nucleotide sequence