Experiment: m.b. Aluminum chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0028 and Dshi_0029 are separated by 85 nucleotides Dshi_0029 and Dshi_0030 are separated by 11 nucleotides Dshi_0030 and Dshi_0031 are separated by 35 nucleotides
Dshi_0028: Dshi_0028 - A/G-specific adenine glycosylase (RefSeq), at 31,815 to 32,909
_0028
Dshi_0029: Dshi_0029 - protein of unknown function DUF1159 (RefSeq), at 32,995 to 33,507
_0029
Dshi_0030: Dshi_0030 - thiol-disulfide oxidoreductase D, putative (RefSeq), at 33,519 to 34,190
_0030
Dshi_0031: Dshi_0031 - tetraacyldisaccharide 4'-kinase (RefSeq), at 34,226 to 35,239
_0031
Position (kb) 33
34
35 Strain fitness (log2 ratio) -2
-1
0
1 at 32.520 kb on - strand, within Dshi_0028 at 32.629 kb on + strand, within Dshi_0028 at 32.678 kb on + strand, within Dshi_0028 at 32.949 kb on + strand at 32.956 kb on + strand at 32.961 kb on + strand at 33.586 kb on - strand at 33.601 kb on - strand, within Dshi_0030 at 33.717 kb on + strand, within Dshi_0030 at 33.759 kb on - strand, within Dshi_0030 at 33.853 kb on + strand, within Dshi_0030 at 33.854 kb on + strand, within Dshi_0030 at 33.863 kb on - strand, within Dshi_0030 at 34.060 kb on + strand, within Dshi_0030 at 34.071 kb on - strand, within Dshi_0030
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Aluminum chloride 0.5 mM remove 32,520 - Dshi_0028 0.64 +1.1 32,629 + Dshi_0028 0.74 -1.1 32,678 + Dshi_0028 0.79 +0.9 32,949 + -0.2 32,956 + -0.7 32,961 + -1.3 33,586 - -1.6 33,601 - Dshi_0030 0.12 -0.2 33,717 + Dshi_0030 0.29 -2.2 33,759 - Dshi_0030 0.36 -0.6 33,853 + Dshi_0030 0.50 -0.5 33,854 + Dshi_0030 0.50 -0.5 33,863 - Dshi_0030 0.51 -1.7 34,060 + Dshi_0030 0.81 -0.4 34,071 - Dshi_0030 0.82 -2.1
Or see this region's nucleotide sequence