Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_661 and Pf6N2E2_662 are separated by 242 nucleotides Pf6N2E2_662 and Pf6N2E2_663 are separated by 4 nucleotides Pf6N2E2_663 and Pf6N2E2_664 are separated by 342 nucleotides
Pf6N2E2_661 - DipZ protein, at 764,968 to 766,179
_661
Pf6N2E2_662 - Valyl-tRNA synthetase, at 766,422 to 766,739
_662
Pf6N2E2_663 - Transcriptional activator of acetoin dehydrogenase operon AcoR, at 766,744 to 768,609
_663
Pf6N2E2_664 - Acetoin catabolism protein X, at 768,952 to 770,025
_664
Position (kb)
766
767
768
769 Strain fitness (log2 ratio)
-2
-1
0
1 at 765.835 kb on + strand, within Pf6N2E2_661 at 765.835 kb on + strand, within Pf6N2E2_661 at 765.836 kb on - strand, within Pf6N2E2_661 at 765.868 kb on + strand, within Pf6N2E2_661 at 765.869 kb on - strand, within Pf6N2E2_661 at 765.937 kb on + strand, within Pf6N2E2_661 at 765.977 kb on + strand, within Pf6N2E2_661 at 765.998 kb on - strand, within Pf6N2E2_661 at 766.051 kb on + strand, within Pf6N2E2_661 at 766.066 kb on + strand at 766.067 kb on - strand at 766.321 kb on + strand at 766.570 kb on - strand, within Pf6N2E2_662 at 766.653 kb on + strand, within Pf6N2E2_662 at 766.653 kb on + strand, within Pf6N2E2_662 at 766.654 kb on - strand, within Pf6N2E2_662 at 766.654 kb on - strand, within Pf6N2E2_662 at 766.654 kb on - strand, within Pf6N2E2_662 at 766.698 kb on + strand, within Pf6N2E2_662 at 766.698 kb on + strand, within Pf6N2E2_662 at 766.698 kb on + strand, within Pf6N2E2_662 at 766.699 kb on - strand, within Pf6N2E2_662 at 766.701 kb on + strand, within Pf6N2E2_662 at 766.701 kb on + strand, within Pf6N2E2_662 at 766.702 kb on - strand, within Pf6N2E2_662 at 766.702 kb on - strand, within Pf6N2E2_662 at 766.738 kb on - strand at 767.192 kb on + strand, within Pf6N2E2_663 at 767.193 kb on - strand, within Pf6N2E2_663 at 767.841 kb on - strand, within Pf6N2E2_663 at 768.248 kb on + strand, within Pf6N2E2_663 at 768.609 kb on + strand at 768.610 kb on - strand at 768.649 kb on + strand at 768.887 kb on - strand at 768.887 kb on - strand at 768.890 kb on - strand at 768.904 kb on + strand at 768.905 kb on - strand at 768.905 kb on - strand at 769.432 kb on + strand, within Pf6N2E2_664 at 769.433 kb on - strand, within Pf6N2E2_664 at 769.433 kb on - strand, within Pf6N2E2_664
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 765,835 + Pf6N2E2_661 0.72 -0.1 765,835 + Pf6N2E2_661 0.72 +1.1 765,836 - Pf6N2E2_661 0.72 +0.6 765,868 + Pf6N2E2_661 0.74 +0.3 765,869 - Pf6N2E2_661 0.74 +0.0 765,937 + Pf6N2E2_661 0.80 +0.7 765,977 + Pf6N2E2_661 0.83 +1.1 765,998 - Pf6N2E2_661 0.85 +1.7 766,051 + Pf6N2E2_661 0.89 -0.6 766,066 + -0.4 766,067 - +0.4 766,321 + -0.9 766,570 - Pf6N2E2_662 0.47 -0.1 766,653 + Pf6N2E2_662 0.73 -0.1 766,653 + Pf6N2E2_662 0.73 -0.8 766,654 - Pf6N2E2_662 0.73 -0.1 766,654 - Pf6N2E2_662 0.73 +1.3 766,654 - Pf6N2E2_662 0.73 -0.1 766,698 + Pf6N2E2_662 0.87 +0.6 766,698 + Pf6N2E2_662 0.87 +0.8 766,698 + Pf6N2E2_662 0.87 -0.1 766,699 - Pf6N2E2_662 0.87 +0.7 766,701 + Pf6N2E2_662 0.88 +0.6 766,701 + Pf6N2E2_662 0.88 -0.6 766,702 - Pf6N2E2_662 0.88 -1.1 766,702 - Pf6N2E2_662 0.88 -1.1 766,738 - +0.1 767,192 + Pf6N2E2_663 0.24 -0.6 767,193 - Pf6N2E2_663 0.24 -0.7 767,841 - Pf6N2E2_663 0.59 +0.2 768,248 + Pf6N2E2_663 0.81 -2.4 768,609 + +0.7 768,610 - -0.9 768,649 + -0.2 768,887 - +0.4 768,887 - -0.5 768,890 - -0.8 768,904 + +0.9 768,905 - -0.6 768,905 - +0.7 769,432 + Pf6N2E2_664 0.45 -0.2 769,433 - Pf6N2E2_664 0.45 +0.4 769,433 - Pf6N2E2_664 0.45 +0.1
Or see this region's nucleotide sequence