Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_5198

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_5194 and Pf6N2E2_5195 are separated by 95 nucleotidesPf6N2E2_5195 and Pf6N2E2_5196 are separated by 7 nucleotidesPf6N2E2_5196 and Pf6N2E2_5197 overlap by 19 nucleotidesPf6N2E2_5197 and Pf6N2E2_5198 are separated by 1 nucleotidesPf6N2E2_5198 and Pf6N2E2_6155 are separated by 778 nucleotidesPf6N2E2_6155 and Pf6N2E2_5199 are separated by 103 nucleotides Pf6N2E2_5194 - Methyl-accepting chemotaxis protein, at 5,989,989 to 5,991,350 _5194 Pf6N2E2_5195 - hypothetical protein, at 5,991,446 to 5,992,000 _5195 Pf6N2E2_5196 - Transcriptional regulator, Cro/CI family, at 5,992,008 to 5,992,241 _5196 Pf6N2E2_5197 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 5,992,223 to 5,992,345 _5197 Pf6N2E2_5198 - Gamma-glutamyltranspeptidase (EC 2.3.2.2), at 5,992,347 to 5,993,582 _5198 Pf6N2E2_6155 - tRNA-Met-CAT, at 5,994,361 to 5,994,434 _6155 Pf6N2E2_5199 - Methyl-accepting chemotaxis protein, at 5,994,538 to 5,996,595 _5199 Position (kb) 5992 5993 5994Strain fitness (log2 ratio) -2 -1 0 1at 5991.349 kb on - strandat 5991.349 kb on - strandat 5991.426 kb on + strandat 5991.426 kb on + strandat 5991.427 kb on - strandat 5991.427 kb on - strandat 5991.451 kb on + strandat 5991.452 kb on - strandat 5991.470 kb on + strandat 5991.670 kb on + strand, within Pf6N2E2_5195at 5991.671 kb on - strand, within Pf6N2E2_5195at 5991.728 kb on + strand, within Pf6N2E2_5195at 5991.743 kb on + strand, within Pf6N2E2_5195at 5991.767 kb on + strand, within Pf6N2E2_5195at 5991.767 kb on + strand, within Pf6N2E2_5195at 5991.767 kb on + strand, within Pf6N2E2_5195at 5991.768 kb on - strand, within Pf6N2E2_5195at 5991.768 kb on - strand, within Pf6N2E2_5195at 5991.889 kb on + strand, within Pf6N2E2_5195at 5991.890 kb on - strand, within Pf6N2E2_5195at 5991.932 kb on - strand, within Pf6N2E2_5195at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.033 kb on + strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.034 kb on - strand, within Pf6N2E2_5196at 5992.082 kb on - strand, within Pf6N2E2_5196at 5992.133 kb on + strand, within Pf6N2E2_5196at 5992.133 kb on + strand, within Pf6N2E2_5196at 5992.133 kb on + strand, within Pf6N2E2_5196at 5992.134 kb on - strand, within Pf6N2E2_5196at 5992.134 kb on - strand, within Pf6N2E2_5196at 5992.134 kb on - strand, within Pf6N2E2_5196at 5992.134 kb on - strand, within Pf6N2E2_5196at 5992.135 kb on + strand, within Pf6N2E2_5196at 5992.135 kb on + strand, within Pf6N2E2_5196at 5992.135 kb on + strand, within Pf6N2E2_5196at 5992.135 kb on + strand, within Pf6N2E2_5196at 5992.136 kb on - strand, within Pf6N2E2_5196at 5992.136 kb on - strand, within Pf6N2E2_5196at 5992.136 kb on - strand, within Pf6N2E2_5196at 5992.136 kb on - strand, within Pf6N2E2_5196at 5992.167 kb on + strand, within Pf6N2E2_5196at 5992.167 kb on + strand, within Pf6N2E2_5196at 5992.168 kb on - strand, within Pf6N2E2_5196at 5992.168 kb on - strand, within Pf6N2E2_5196at 5992.168 kb on - strand, within Pf6N2E2_5196at 5992.168 kb on - strand, within Pf6N2E2_5196at 5992.200 kb on + strand, within Pf6N2E2_5196at 5992.201 kb on - strand, within Pf6N2E2_5196at 5992.412 kb on - strandat 5992.503 kb on + strand, within Pf6N2E2_5198at 5992.504 kb on - strand, within Pf6N2E2_5198at 5992.790 kb on + strand, within Pf6N2E2_5198at 5992.790 kb on + strand, within Pf6N2E2_5198at 5992.790 kb on + strand, within Pf6N2E2_5198at 5992.816 kb on + strand, within Pf6N2E2_5198at 5992.865 kb on + strand, within Pf6N2E2_5198at 5992.865 kb on + strand, within Pf6N2E2_5198at 5992.865 kb on + strand, within Pf6N2E2_5198at 5992.866 kb on - strand, within Pf6N2E2_5198at 5992.957 kb on + strand, within Pf6N2E2_5198at 5992.957 kb on + strand, within Pf6N2E2_5198at 5992.958 kb on - strand, within Pf6N2E2_5198at 5992.958 kb on - strand, within Pf6N2E2_5198at 5993.182 kb on + strand, within Pf6N2E2_5198at 5993.182 kb on + strand, within Pf6N2E2_5198at 5993.183 kb on - strand, within Pf6N2E2_5198at 5993.183 kb on - strand, within Pf6N2E2_5198at 5993.218 kb on + strand, within Pf6N2E2_5198at 5993.219 kb on - strand, within Pf6N2E2_5198at 5993.219 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.306 kb on - strand, within Pf6N2E2_5198at 5993.413 kb on + strand, within Pf6N2E2_5198at 5993.625 kb on + strandat 5993.702 kb on + strandat 5993.702 kb on + strandat 5993.702 kb on + strandat 5993.703 kb on - strandat 5993.703 kb on - strandat 5993.703 kb on - strandat 5993.985 kb on + strandat 5994.001 kb on + strandat 5994.002 kb on - strandat 5994.002 kb on - strandat 5994.002 kb on - strandat 5994.060 kb on + strandat 5994.060 kb on + strandat 5994.061 kb on - strandat 5994.061 kb on - strandat 5994.061 kb on - strandat 5994.096 kb on + strandat 5994.096 kb on + strandat 5994.096 kb on + strandat 5994.097 kb on - strandat 5994.196 kb on + strandat 5994.196 kb on + strandat 5994.197 kb on - strandat 5994.331 kb on - strandat 5994.490 kb on - strandat 5994.514 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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5,991,349 - -0.3
5,991,349 - +0.9
5,991,426 + -0.1
5,991,426 + -0.5
5,991,427 - +0.1
5,991,427 - -0.7
5,991,451 + -0.3
5,991,452 - -0.2
5,991,470 + +0.9
5,991,670 + Pf6N2E2_5195 0.40 +0.2
5,991,671 - Pf6N2E2_5195 0.41 +0.4
5,991,728 + Pf6N2E2_5195 0.51 +0.4
5,991,743 + Pf6N2E2_5195 0.54 +0.2
5,991,767 + Pf6N2E2_5195 0.58 +0.2
5,991,767 + Pf6N2E2_5195 0.58 -0.5
5,991,767 + Pf6N2E2_5195 0.58 +0.9
5,991,768 - Pf6N2E2_5195 0.58 -0.6
5,991,768 - Pf6N2E2_5195 0.58 +0.4
5,991,889 + Pf6N2E2_5195 0.80 +0.9
5,991,890 - Pf6N2E2_5195 0.80 -0.8
5,991,932 - Pf6N2E2_5195 0.88 +0.8
5,992,033 + Pf6N2E2_5196 0.11 +0.8
5,992,033 + Pf6N2E2_5196 0.11 +1.0
5,992,033 + Pf6N2E2_5196 0.11 +0.5
5,992,033 + Pf6N2E2_5196 0.11 +0.4
5,992,033 + Pf6N2E2_5196 0.11 +0.1
5,992,033 + Pf6N2E2_5196 0.11 +0.2
5,992,033 + Pf6N2E2_5196 0.11 +0.5
5,992,033 + Pf6N2E2_5196 0.11 -2.0
5,992,033 + Pf6N2E2_5196 0.11 +0.7
5,992,033 + Pf6N2E2_5196 0.11 -0.5
5,992,033 + Pf6N2E2_5196 0.11 -0.0
5,992,033 + Pf6N2E2_5196 0.11 -1.2
5,992,034 - Pf6N2E2_5196 0.11 -0.7
5,992,034 - Pf6N2E2_5196 0.11 +1.1
5,992,034 - Pf6N2E2_5196 0.11 +0.3
5,992,034 - Pf6N2E2_5196 0.11 -0.2
5,992,034 - Pf6N2E2_5196 0.11 -1.2
5,992,034 - Pf6N2E2_5196 0.11 +0.3
5,992,034 - Pf6N2E2_5196 0.11 +0.8
5,992,034 - Pf6N2E2_5196 0.11 +0.9
5,992,082 - Pf6N2E2_5196 0.32 -0.3
5,992,133 + Pf6N2E2_5196 0.53 -1.0
5,992,133 + Pf6N2E2_5196 0.53 -0.1
5,992,133 + Pf6N2E2_5196 0.53 -0.4
5,992,134 - Pf6N2E2_5196 0.54 -0.1
5,992,134 - Pf6N2E2_5196 0.54 +0.7
5,992,134 - Pf6N2E2_5196 0.54 -1.5
5,992,134 - Pf6N2E2_5196 0.54 -0.2
5,992,135 + Pf6N2E2_5196 0.54 +0.8
5,992,135 + Pf6N2E2_5196 0.54 -1.0
5,992,135 + Pf6N2E2_5196 0.54 +0.2
5,992,135 + Pf6N2E2_5196 0.54 +0.2
5,992,136 - Pf6N2E2_5196 0.55 -0.1
5,992,136 - Pf6N2E2_5196 0.55 -0.7
5,992,136 - Pf6N2E2_5196 0.55 -1.5
5,992,136 - Pf6N2E2_5196 0.55 -1.6
5,992,167 + Pf6N2E2_5196 0.68 -0.5
5,992,167 + Pf6N2E2_5196 0.68 -0.2
5,992,168 - Pf6N2E2_5196 0.68 +1.0
5,992,168 - Pf6N2E2_5196 0.68 -1.7
5,992,168 - Pf6N2E2_5196 0.68 -1.0
5,992,168 - Pf6N2E2_5196 0.68 +0.9
5,992,200 + Pf6N2E2_5196 0.82 -0.3
5,992,201 - Pf6N2E2_5196 0.82 -0.9
5,992,412 - -0.9
5,992,503 + Pf6N2E2_5198 0.13 -0.1
5,992,504 - Pf6N2E2_5198 0.13 +0.9
5,992,790 + Pf6N2E2_5198 0.36 +1.1
5,992,790 + Pf6N2E2_5198 0.36 -2.0
5,992,790 + Pf6N2E2_5198 0.36 -0.5
5,992,816 + Pf6N2E2_5198 0.38 +0.8
5,992,865 + Pf6N2E2_5198 0.42 +1.1
5,992,865 + Pf6N2E2_5198 0.42 -1.2
5,992,865 + Pf6N2E2_5198 0.42 +0.3
5,992,866 - Pf6N2E2_5198 0.42 +0.8
5,992,957 + Pf6N2E2_5198 0.49 +0.9
5,992,957 + Pf6N2E2_5198 0.49 -0.2
5,992,958 - Pf6N2E2_5198 0.49 -1.3
5,992,958 - Pf6N2E2_5198 0.49 +0.3
5,993,182 + Pf6N2E2_5198 0.68 +1.0
5,993,182 + Pf6N2E2_5198 0.68 +0.8
5,993,183 - Pf6N2E2_5198 0.68 -0.1
5,993,183 - Pf6N2E2_5198 0.68 +1.4
5,993,218 + Pf6N2E2_5198 0.70 +1.5
5,993,219 - Pf6N2E2_5198 0.71 +0.0
5,993,219 - Pf6N2E2_5198 0.71 -1.0
5,993,306 - Pf6N2E2_5198 0.78 +0.2
5,993,306 - Pf6N2E2_5198 0.78 +0.9
5,993,306 - Pf6N2E2_5198 0.78 +0.7
5,993,306 - Pf6N2E2_5198 0.78 -0.9
5,993,306 - Pf6N2E2_5198 0.78 -0.5
5,993,306 - Pf6N2E2_5198 0.78 -0.2
5,993,306 - Pf6N2E2_5198 0.78 +0.6
5,993,413 + Pf6N2E2_5198 0.86 +0.3
5,993,625 + +0.4
5,993,702 + +0.4
5,993,702 + -0.7
5,993,702 + +1.1
5,993,703 - -0.3
5,993,703 - -0.3
5,993,703 - -2.3
5,993,985 + -0.8
5,994,001 + +0.0
5,994,002 - +0.9
5,994,002 - -0.7
5,994,002 - +0.2
5,994,060 + +0.6
5,994,060 + -0.5
5,994,061 - -0.3
5,994,061 - +0.8
5,994,061 - -0.6
5,994,096 + -0.1
5,994,096 + +0.0
5,994,096 + +0.2
5,994,097 - +0.4
5,994,196 + +1.4
5,994,196 + -0.6
5,994,197 - +0.3
5,994,331 - +0.1
5,994,490 - +0.3
5,994,514 - +0.6

Or see this region's nucleotide sequence